• 제목/요약/키워드: germination experiment

검색결과 528건 처리시간 0.02초

온도 , 광 및 Gibberellic Acid 처리가 참꽃나무의 종자발아에 미치는 영향 (The effects of temperature, light and gibberellic acid on seed germination of Rhododendron weyrichii Max.)

  • 황환주
    • 한국자원식물학회지
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    • 제8권1호
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    • pp.41-46
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    • 1995
  • 조경용(造景用) 및 분재용(栽用) 화목(花木)으로 개발가치가 매우 높은 참꽃나무의 종자발아특성(種子發芽特性)에 관하여 실험한 결과는 다음과 같다. 1. 항온조건(恒溫條件)에서의 발아적온(發芽適溫)은 $20^{\circ}C$이었고 $25^{\circ}C$ 이상의 온도에서는 발아가 심하게 억제되었다. 한편 $15^{\circ}C$에서는 초기발아노(初期發芽努)는 저조하였지만 최종발아율은 $20^{\circ}C$에서와 거의 비슷하였다. 2. 야간온도(夜間溫度)를 $15^{\circ}C$로 고정한 변온처리(變溫處理)를 통하여 $25^{\circ}C$$30^{\circ}C$의 고온처리구(高溫處理區)에서 항온처리에 비하여 발아율이 현저하게 높아겼다. 3. 황색(黃色) 및 적색광(赤色光) 처리구(處理區)에서는 대조구(對照區)에 비하여 유의성(有意性)있게 발아율이 증가하였고, 녹색광(綠色光) 처리구(處理區)에서는 발아율이 현저하게 억제되었다. 또한 암처리구(暗處理區)에서는 전혀 발아하지 못하여 참꽃나무 종자는 절대적(絶對的) 광발아성(光發芽性) 종자(種子)임이 확인되었다. 4. 암조건(暗條件)에서도 $50{\sim}100ppm$ 농도(濃度)의 GA처리에 의하여 발아(發芽)가 가능하여 GA의 광대체효과(光對替效果)가 확인되었다.

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시호 종자의 특성 및 발아촉진 방법 연구 (Seed Characteristics and Accelerating Method of Germination in Bupleurum falcatum)

  • 이은일;김석현
    • 한국작물학회지
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    • 제41권3호
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    • pp.384-394
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    • 1996
  • 시호 종자의 발아율을 증가시키기 위한 방법과 종자의 특징을 연구하기 위하여 본 실험을 수행하였다. 시호의 발아에 유리한 온도는 2$0^{\circ}C$이며, 호르몬 처리나 기타 물리화학적 처리에 의해 발아율은 크게 증가되지 못했는데, 15$^{\circ}C$에서 발아시킬 경우 50~200 ppm의 GA$_3$을 처리했을 때 발아율이 2배 이상 증가하였다. 또한 같은 온도에서 $10^{-2}$ ~$10^{-3}$M의 KNO$_3$을 처리한 경우에도 발아율은 3배 이상 증가하였다. 그러나 2$0^{\circ}C$ 이상에서는 강력한 저해 효과를 나타내었다. 시호의 leachate를 상추의 종자에 처리했을 경우 발아율의 변화가 거의 없었으므로 시호의 종자에는 발아저해제가 거의 없는 것으로 생각된다. 해부현미경과 주사전자현미경으로 종자의 배와 주공을 관찰한 결과 배가 있는 것과 없는 것의 비율이 거의 50/50이며 주공 자체에는 문제가 없으므로 시호의 종자 발아율이 낮은 것은 근본적으로 배가 결여된 종자가 많기 때문인 것으로 생각된다.

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파종전후 종자에 가해지는 광질, $GA_3$ 및 온도에 따른 담배의 발아율 (Effect of Light Quality,$GA_3$ and Temperature as Treatments Before or During Germination on Tobacco Seed Germinability)

  • 강진호
    • 한국자원식물학회지
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    • 제11권2호
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    • pp.124-130
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    • 1998
  • It failed occasionally to take a reasonable emergence rate since tobacco (Nicotiana tabacum L.) seeds were planted on late Jan. showing lowest temperature. This experiment was done to measure the effect of GA3 (concentration ; period) , light quality (red ; white ; dark) during or after its treatment, daily irradiation hours( 0 ; 8 ; 12 ;16) and germination termperature (20 or 10 $^{\circ}C$ ocnstant ; 20/1$0^{\circ}C$ alternating) on the germination rate. Red and white light given during grmination showed no differences between the other daily irradiation hours except that 8 hours red light delayed germination although their 12 hours irradiation had the gratest rate. The rate was increased with increased concentration to GA3 0.01 mM or increased imbibition period to 3 days although the rate of cv. NC 82 was less than that of cv. Burley 21 in the case of dark imbibition of GA3 but daily 12 hours irradiation during germination. Light quality forced during GA3 imbibition eliminated such effect of GA3 shown in the darkness so that only light quality pretreatment and termperature during germinition were affected on the rate. The germination rate of thecultivars was decreased in the order of red, white light, darkness meaning that it was highly influenced by the light quality during GA3 treatment. Regardless of GA3 or light quality treatment,on the other hand, the rate was greater in 20 $^{\circ}C$ constat than 1$0^{\circ}C$ constant and 20/1$0^{\circ}C$ alternating germination temperature having similar germinative patterns.

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GA3와 냉습저장, 배병의 제거가 금낭화의 발아에 미치는 영향 (Effects of GA3, Moist-Chilling Storage and Removal of Funiculus on the Germination of Dicentra spectabilis)

  • 김홍열;정재동
    • 원예과학기술지
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    • 제19권1호
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    • pp.78-80
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    • 2001
  • 금낭화의 실생번식방법을 확립하기 위하여 $GA_3(100mg{\cdot}L^{-1})$와 냉습저장($3^{\circ}C$) 그리고 배병의 제거가 금낭화의 발아에 미치는 영향을 조사하였다. $GA_3$를 처리하지 않은 종자는 90일간의 냉습저장에도 발아를 하지 않았다. 그러나 $GA_3$처리는 냉습저장에 관계없이 금낭화의 발아를 촉진하였다. 배병을 제거한 경우 $GA_3$처리에 의한 발아촉진효과가 감소하는 경향을 나타내었다. $GA_3$처리 후 냉습저장에 의해서 또한 냉습저장 기간이 길어질수록 발아일수는 단축되었으며 발아속도는 촉진되었다. 이상의 결과로부터 $GA_3$처리는 금낭화의 발아촉진에 효과적이며 냉습저장처리는 $GA_3$의 효과에 상승적인 작용을 하는 것을 알 수 있었다.

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염생식물 칠면초(Suaeda japonica)의 종자발아와 유묘생장에 종자 보관기간과 토양구성이 미치는 영향 (Effects of Seed Preservation Periods and Soil Composition on the Seed Germination and Seedling Growth of Halophyte Suaeda japonica)

  • 곽용성;김시은;오지철;박서경;최한길
    • Ocean and Polar Research
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    • 제44권4호
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    • pp.311-317
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    • 2022
  • Seed germination and seedling growth of a halophyte, Suaeda japonica were examined to measure the effects of seed preservation periods and various soil compositions in the mudflat of Saemangeum, Korea. Seed germination declined from 74.30% to 44.30% as the preservation period increased from 4 months to 12 months. After 14 days of seeding, seed germination was lowest in sandy soil (SS) at 8.67% and highest in earthworm cast+leaf mold (EC+LM) at 67.33%, showing 4~5 times higher than SS. The average lengths of seedlings were 12.20 mm in SS and 42.20 mm in EC+LM, respectively. These results suggest that seed germination and seedling growth enhanced in the soil that has more organic matter. In the experiment mixing Saemangeum soil (SMS), seed germination was from 4.00% in SMS to 88.00% in the soil of SMS+EC+LM after 14 days. Similar growth patterns were observed in the length of seedlings from 5.00 mm in SMS to 49.60 mm in SMS+EC+LM. The present results indicate that a population of Suaeda japonica could be established by stimulating seed germination and seedling growth by the addition of organic matters such as EC, LM, and EC+LM in the Saemangeum mud flat.

한국(韓國) 자생(自生) 새우난초 종자(種子)의 무균발아(無菌發芽) (Asymbiotic Germination of Korean Native Calanthe Species)

  • 김창길;정재동
    • Current Research on Agriculture and Life Sciences
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    • 제15권
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    • pp.47-52
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    • 1997
  • 새우난초, 금새우난초 및 이들 교잡종자(交雜種子)의 기내(機內) 무균발아(無菌發芽)에 적합(適合)한 기내(機內) 배양조건(培養條件)을 구명(究明)하기 위하여 수분후(受粉後) 일수(日數), 기본배지(基本培地), 초음파(超音波) 처리효과(處理效果) 실험(實驗)을 수행(遂行)하였다. 수분후(受粉後) 100일(日)된 새우란 종자(種子)를 MS배지에 파종(播種)하였을 때 발아율(發芽率)이 가장 양호(良好)하였으며 초음파(超音波) 처리시간별(處理時間別)로는 10분간(分間) 처리(處理)한 다음 파종(播種)하였을 때 발아소요일수(發芽所要日數)는 단축(短縮)되고 발아후(發芽後) 유묘(幼苗)의 생육(生育)도 양호(良好)한 경향(傾向)이었다.

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칡의 대량 생산이용 및 생리활성물질에 관한 연구 I. 진한황산 처리가 칡 종자의 발아 및 생육에 미치는 영향 (Studies on Mass Production , Utilization and Bioactive Substance of Kudzu I. The effect of concentrated sulfate on germination rate and growth chracte in kudzu)

  • 정대수;김대진
    • 한국초지조사료학회지
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    • 제13권3호
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    • pp.190-194
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    • 1993
  • This experiment was conducted to investigate the effects of concentrated sulfate on the germination rate, growth rate and the productivity of weight of kudzu (Pueraria thwnbergiana Bentham). The germination rate of kudzu seed by one or two treatment retreatment with the concentrated sulfate for minutes of 5. 10, 15 and 20 were determined respectively. The results obtained were summarized as follows; 1. In the time of concentrated sulfate treatment, the germination rate was the highest in the once treatment for 20 minute. and the retreatment was higher than the once treatment in the germination rate. 2. The changes of plant length by growth stage was grown gradually until for 60 day and rapidly after for 60 day. The growth rate was the highest in treatment for 15 minute than in other treatments. 3. The stem diameter. stem weight. fresh weight and root weight by the treatment with sulfate 15 minute as the treatment with once were high degree of productivity. 4. The number of branch. number of nodes and root weight by the growth stage were higher growth rate before for 90 day than after for 90 day, however stem weight and fresh weight were high degree of productivity after for 60 day.

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Exploitation of the biologically active substances in germinating Mung bean and Buckwheat seeds

  • Back, Jong-Oh;Lee, Sook-Young;Hwang, Eun-Joo;Boo, Hee-Ock;Pyo, Byoung-Sik
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2003년도 춘계 학술발표대회
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    • pp.103-103
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    • 2003
  • This study was carried out to investigate of the biologically active components in germinating Mung bean(Phaseolus radiata L.) and Buckwheat (Fagopyrum esculentum Moench) seeds. During the initial germination, germination ratio of 24 hours pre-soaking Mung bean and Buckwheat seeds were higher about 2∼3% than that of non-soaking. This experiment also was peformed to observe cytotoxic effect of the germinating seeds(germination length : 2, 5, 10mm) extracts against cancer cell lines including human lung carcinoma(Calu-6), human breast adenocarcinoma(MCT-7), human great intestine carcinoma(Caco-2) and human leukemia carcinoma(AML-2/WT). The growth of the cancer cells in medium containing Mung bean and Buckwheat extracts were significantly inhibited degree in proportion to the length of germination seeds, Especially, the results show that a significant shrinkage of Calu-6 cells was observed when the cells were exposed into extract of 10mm germination seeds in germinating Mung bean and Buckwheat seeds.

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