• 농업과학연구
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• 제47권3호
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• pp.693-705
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• 2020

Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna^® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.

• 한국어병학회지
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• 제29권1호
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• pp.7-12
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• 2016

In a previous study on the parasites of cultured rock bream (Oplegnathus fasciatus), we reported the discovery of a new species, Ceratomyxa oplegnathus, obtained from the gallbladder. In the present study, we found another Ceratomyxa species, C. sparusaurati, also from the gallbladder of rock bream. The morphological and ultrastructural characteristics of C. sparusaurati were investigated using light and transmission electron microscopy (TEM).

• 한국동물학회지
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• 제8권2호
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• pp.15-18
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• 1965

Fatty acid components of the Chestnut gall wasp (Dryocosmus kuriphilus YASUMATSU) were analyzed by the method of gas chromatogrphy at various developmental stages. 1. The fatty acids identified at each stage are as follows : Larva stage : acetic acid , benzoic acid and capric acid. Prepupa stage : acetic acid, benzoic acid and capric acid. Pupa stage : acetic acid, benzoic acid and capric acid. 2. Through the development of the wasp, there were large amount of acetic acid.

• 미생물학회지
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• 제4권2호
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• pp.5-10
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• 1966

The relationships between tumor score and peroxidase activities of tomato stems infected with Agrobacterium tumefaciens strain A6Kl, B6, T372 11BNV6, 11BV7 and wounded stem as a control were examined in relation to crown gall tumor development on purpose to study the lignification of tumor tissue which is affected to the development of crown gall tumor. As the previous paper has been mentioned the fact that the induction of tumor tissues were inhibited or limited in the lignified stem of host plant. It was presumed that the activities of peroxidase related to the development of lignification were decreased during the period of tumor development. But the experimental result in this experiment shows that the peroxidase activities of crown gall tumor-tissues infected with the A. tumefaciens strains which are already known as virulent are increasing during four weeks, however, in the strain 11BNV6 and wound the peroxidase activities are decreasing on the second week after the inoculation of the bacteria strains. These results could be explained on the basis of that possible regulatory agents of lignification which were accumulated in tumor tissues, IAA, ascorbic acid, glutathion(GSH) and caffeic acid esters, were postulated to act as antioxidants which has been suggested by Stafford. Total nitrogen contents in relation to crown gall tumor development were determined for the detection of protein synthesis related to the enzyme activities which are increasing in the time of plant growth. Generally six groups are contained the largest amount of nitrogen on the second week after the inoculation of the bacterium. Comparing to the tumor score, it is presumed that the all of enzyme activities including peroxidase in tumor tissues are increasing from the second week through the third week after the inoculation of bacterium and the protein synthesis is stimulated under the most appropriated temperature during the above periods.

• Applied Biological Chemistry
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• 제23권3호
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• pp.189-197
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• 1980

소나무의 monoterpene조성(組成)가 솔잎혹파리에 대(對)한 저항성(抵抗性) 관계(關係)를 구명(究明)하기 위(爲)하여 솔잎혹파리 저항성(抵抗性) 소나무 5개(5個) 수종(樹種)가 감수성(感受性)소나무 2개(2個) 수종(樹種), 그리고 감수성(感受性) 해송(海松)가운데 피해목(被害木)과 선발목(選拔木)의 침엽(針葉)을 GLC로 분석(分析)하여 monoterpenes의 조성(組成)을 근거(根據)로 저항성(抵抗性) 또는 감수성(感受性) 수종(樹種)을 구별(區別)할 수는 없었다. 그러나 적송수종내(赤松樹種內)의 피해목(被害木)과 선발목간(選拔木間)에는 limonene, ${\alpha}-pinene$, myrcene 및 ${\beta}-phellandrene$의 농도(濃度)아 조성비(組成比)에 차이(差異)가 인정(認定)되였다. 적송(赤松)의 선발목(選拔木)에서 limonene의 농도(濃度)는 솔잎혹파리 산란이후(産卵以後) 점차(漸次) 증가(增加)하였으나 피해목(被害木)에서는 증가(增加)하지 않았다. 적송(赤松)의 선발목(選拔木)에서 솔잎혹파리에 대(對)한 내충성분은limonene으로 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2923-2928
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• 2015

Background: Gallbladder carcinoma (GBC) has been stated as an Indian disease, with the highest number of cases being reported from certain districts of northeast India, which has an ethnically distinct population. Unfortunately there are no scientific reports on the underlying molecular mechanisms associated with the pathogenesis of the disease from this region. Aim: The present study evaluated the role of differential expression of human telomerase reverse transcriptase (hTERT) in the development of gall bladder anomalies. Materials and Methods: Blood and tissue samples were collected from patients undergoing routine surgical resection for clinically proven cases of gallbladder disease {cholelithiasis (CL, n=50), cholecystitis (CS, n=40) and GBC (n=30) along with adjacent histopathologically proved non-neoplastic controls (n=15)} with informed consent. Whole blood was also collected from age and sex matched healthy controls (n=25) for comparative analysis. Differential hTERT mRNA expression was evaluated by semi-quantitative rt-PCR and real-time PCR based analysis using ${\beta}$-actin as an internal control. Evaluation of differential hTERT protein expression was studied by Western blot analysis and immunoflourescence. Statistical analysis for differential expression and co-relation was performed by SPSSv13.0 software. Results: Gallbladder anomalies were mostly prevalent in females. The hTERT mRNA and protein expression increased gradiently from normal

• Journal of Acupuncture Research
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• 제22권5호
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• pp.29-36
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• 2005

Objectives : This study is performed to understand the interrelation between 'Foot soyang muscle of the Gall bladder channel' and 'muscular system' on the basis of the link between meridian muscle theory and myofascial pain syndrome. Methods : We have researched some of oriental medical books about meridian muscle theory and western medical books about anatomical muscular system. Results & Conclusion : 1. Myofascial pain syndrome is the medical treatment which finds the start point of the pain in fascia and then treats it on the basis of object and concrete anatomical theory, so its application is needed for objectification of the oriental medicine. 2. There is a wide difference between myofascial pain syndrome and meridian muscle theory in that the former explains each muscle individually, while the latter classifies muscles systematically in the view of organism. 3. Foot soyang muscle contains Dorsal interosseous m, Extensor digitorum longus m, Musculus peroneus brevis, longus and, tertius, lliotibial tract, Vastus lateralis m, Gluteus m, Aximus m, Piriformis m, Tensor fasciae latae m, Gluteus minimus m, Obliquus internus & externus abdominis m, External & Internal intercostal m, Serratus anterior m, Pectoralis major m, Sternocleidomastoid m, Auricularis posterior m, Temporalis m, Masseter m, Orbicularis oculi m etc. on the basis of function and the nature of a disease reflected in muscle. 4. Foot soyang muscle keeps the balance of left md right of the body on the outside, while the Gall bladder keeps the balance of the JangBuKiHyeul(臟腑氣血) on the inside.

• 식물병연구
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• 제12권3호
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• pp.284-286
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• 2006

경남 진주시 정촌면 강주연못, 금산면 가정집 정원수로 심은 영산홍 잎에 떡병이 심하게 발생하였다. 병징은 잎에 발생하여 흰색의 가루같은 자실층이 생기고 이상비대를 한다. 병반부에서 자실체가 잎 앞, 뒷면으로 노출되고 흰색의 전형적인 떡모양으로 된다. 담자기는 무색, 곤봉 모양의 원주형이며 $1{\sim}5$개 정도의 작은 자루모양을 하고 있으며 크기는 $8{\sim}35{\times}3{\sim}6{\mu}m$였다. 담자포자는 무색, 단포이며 장타원형 모양의 원주형으로 아랫부분은 약간 굽었으며 크기는 $10{\sim}22{\times}3{\sim}5{\mu}m$였다. 영산홍에서 병원성을 검정하였다. 잎에 발생한 병징과 병원균의 균학적 특징을 조사한 결과, 이 병을 Exobasidium japonicum Shirai에 의한 영산홍 떡병으로 명명하여 국내에 처음 보고한다.

• 한국임상수의학회지
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• 제14권1호
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• pp.11-18
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• 1997

This study was performed to make fundamental data of ultrasonographic diagnosis for liver and duodenum disease in native Korean cattle. Ultrasonographic appearance of liver, caudal vena cava, portal vein, gall bladdr and duodenum according to the growth were determined from 6 to 13 months by monthly examinations in 9 native Korean cattle. The caudal vena cava was determined at 11th intercostal space by use of ultrasonography. The portal vein and gallbladder were determined at 10th intercostal space by use oof ultrasonography. The liver were determined at 12, 11 and 10 th intercostal space by use of ultrasonography. The liver, caudal vena cava, portal cein, gallbladder and duodenum were examinde in standing position. Ultrasonograms were obtainde with 3.5 or 5.0-MHz convex and 5.0-MHz sector transducer. The diameter of vena cava at the 6, 7, 8, 9, 10, 11, 12 and 13 months old age were 18.4, 20.6, 22.4, 25.1, 26.9, 27.3, 28.5 and 29.4 mm, respectively. The diameter of portal vein at the 6, 7, 8, 9, 10, 11, 12 and 13 months old age were 19.5, 20.6, 22.3, 24.5, 26.9, 28.1, 30.7 and 31.5 mm, respectively. The diameter of gall bladder at the 6, 7, 8, 9, 10, 11, 12 and 13 months old age were 63.2, 72.2, 75.1, 78.6, 80.5, 82.3, 84.4 and 91.1 mm, respectively. Cranial part of duodenum was identified near gall bladder by moving of hyperechoic ingesta. Descending duodenum paralleled with vertebrae is seen in the right upper flank and was adjacent to the abdominal wall, and was enveloped in the hyperechoic greater omentum, differentiating it from the jejunum and ileum. The diameter of cranial duodenum at the 6, 7, 8, 9, 10, 11, 12 and 13 months old age were 23.3, 27.9, 29.9, 32.2, 34.4, 34.5, 35.1 and 36.5 mm, respectively. The diameter of descending duodenum revealed smaller diameter than that of cranial duodenum. Calculi in gallbladder were observed by ultrasonography.

• 한국균학회지
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• 제43권4호
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• pp.286-289
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• 2015

순활물기생체인 Plasmodiophora brassicae는 병원성 검정을 위해서 반드시 뿌리혹을 장기적으로 보관하는 것이 매우 중요하기 때문에 그동안 병원성을 유지하는 것이 관건이다. 특히 기존의 방법은 100년 이상 사용되어온 저장법으로 개선이 필요하여 그 효율적인 방법을 밝히고자 하였다. 이 결과 장기적으로 병원성의 저하를 최소화하며 장기간 뿌리혹을 저장할 수 있는 방법은 $-70^{\circ}C$ 냉동고에 보관하는 것으로 확인되었고, 저장 조건은 뿌리혹을 그대로 보관하거나 뿌리혹을 갈아 균질화한 후 여러 겹의 거즈에 거른 것이 6가지 저장조건 중에 가장 효과적인 저장법으로 밝혀졌다.