The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Optimal Storage Condition of Clubroot Pathogen, Plasmodiophora brassicae for Artificial Inoculation

배추뿌리혹병균(Plasmodiophora brassicae)의 인공접종을 위한 효율적인 저장조건

  • Yang, Seul Gi (Department of Applied Biology, Chungnam National University) ;
  • Park, Ju Young (Department of Applied Biology, Chungnam National University) ;
  • Seo, Mun Won (Department of Applied Biology, Chungnam National University) ;
  • Kim, Hong Gi (Department of Applied Biology, Chungnam National University)
  • 양슬기 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 박주영 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 서문원 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김홍기 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2015.08.04
  • Accepted : 2015.11.30
  • Published : 2015.12.31
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Abstract

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is a severe soilborne disease of Brassicaceae. Storage of clubroot gall is important for studies on pathogenicity and race identification. As the current storage method has been used for more than 100 years, a new storage method should be developed and the most efficient way maintaining pathogenicity should be determined. Effects of storage conditions with different storage periods on pathogenicity in galls of kimchi cabbage were examined in a greenhouse. The experiments were performed under six conditions and four temperatures in order to determine the most effective storage conditions for maintenance of pathogenicity. The most effective conditions for clubroot gall storage was the storage of whole gall at $-70^{\circ}C$ or storage of filtrate at the same temperature through eight layers of gauze after homogenization of the galls.

순활물기생체인 Plasmodiophora brassicae는 병원성 검정을 위해서 반드시 뿌리혹을 장기적으로 보관하는 것이 매우 중요하기 때문에 그동안 병원성을 유지하는 것이 관건이다. 특히 기존의 방법은 100년 이상 사용되어온 저장법으로 개선이 필요하여 그 효율적인 방법을 밝히고자 하였다. 이 결과 장기적으로 병원성의 저하를 최소화하며 장기간 뿌리혹을 저장할 수 있는 방법은 $-70^{\circ}C$ 냉동고에 보관하는 것으로 확인되었고, 저장 조건은 뿌리혹을 그대로 보관하거나 뿌리혹을 갈아 균질화한 후 여러 겹의 거즈에 거른 것이 6가지 저장조건 중에 가장 효과적인 저장법으로 밝혀졌다.

Keywords

References

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