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http://dx.doi.org/10.7314/APJCP.2015.16.7.2923

Prognostic Relevance of Human Telomerase Reverse Transcriptase (hTERT) Expression in Patients with Gall Bladder Disease and Carcinoma  

Deblakshmi, Raj Kumari (Department of Biotechnology, Gauhati University)
Deka, Manab (Department of Biotechnology, Gauhati University)
Saikia, Anjan Kumar (Medicinal and Gastroenterology Unit, Central Hospital NF Railway)
Sharma, Bir Kumar (Department of General Surgery, Regional Institute of Medical Sciences)
Singh, Nidhi (Department of Biotechnology, Gauhati University)
Das, NN (Department of HPB and Liver Transplant Surgery, Downtown Hospital)
Bose, Sujoy (Department of Biotechnology, Gauhati University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.7, 2015 , pp. 2923-2928 More about this Journal
Abstract
Background: Gallbladder carcinoma (GBC) has been stated as an Indian disease, with the highest number of cases being reported from certain districts of northeast India, which has an ethnically distinct population. Unfortunately there are no scientific reports on the underlying molecular mechanisms associated with the pathogenesis of the disease from this region. Aim: The present study evaluated the role of differential expression of human telomerase reverse transcriptase (hTERT) in the development of gall bladder anomalies. Materials and Methods: Blood and tissue samples were collected from patients undergoing routine surgical resection for clinically proven cases of gallbladder disease {cholelithiasis (CL, n=50), cholecystitis (CS, n=40) and GBC (n=30) along with adjacent histopathologically proved non-neoplastic controls (n=15)} with informed consent. Whole blood was also collected from age and sex matched healthy controls (n=25) for comparative analysis. Differential hTERT mRNA expression was evaluated by semi-quantitative rt-PCR and real-time PCR based analysis using ${\beta}$-actin as an internal control. Evaluation of differential hTERT protein expression was studied by Western blot analysis and immunoflourescence. Statistical analysis for differential expression and co-relation was performed by SPSSv13.0 software. Results: Gallbladder anomalies were mostly prevalent in females. The hTERT mRNA and protein expression increased gradiently from normal
Keywords
Gall bladder carcinoma; cholelithiasis; cholecystitis; human telomerase reverse transcriptase; PCR;
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1 Baez S, Tsuchiya Y, Calvo A, et al (2010). Genetic variants involved in gallstone formation and capsaicin metabolism, and the risk of gallbladder cancer in Chilean women. World J Gastroenterol, 16, 372-8.   DOI
2 Baichoo E, Boardman LA (2014). Toward a molecular classification of colorectal cancer :the role of telomere length. Front Oncol. 4, 158.
3 Blasco MA, Hahn WC (2003). Evolving views of telomerase and cancer. Trends Cell Biol. 13, 289-4.   DOI
4 Cerni C (2000). Telomeres, telomerase, and myc. An update. Mutat Res, 462, 31-7.   DOI
5 Cesare AJ, Reddel RR (2010). Alternative lengthening of telomeres. Models, mechanisms, and implications. Nat Rev Genet, 11, 319-30.   DOI
6 Cheng YB, Guo LP, Yao P, et al (2014). Telomerase and hTERT: Can they serve as markers for gastric cancer diagnosis? World J Gastroenterol, 20, 6615-9   DOI
7 David L, Nahrwold DL, Dawes LG (1997). Biliary neoplasms in surgery In: Greenfield LJ (ed) Scientific Principles and Practice, 2nd Edn. Philadelphia Raven, pp 1056-6.
8 Feng J, Funk WW, Wang SS, et al (1984). The RNA component of human telomerase. Science, 269, 1236-41.
9 Fujita Y, Fujikane T, Fujiuchi S, et al (2003). The diagnostic and prognostic relevance of human telomerase reverse transcriptase mRNA expression detected in situ in patients with non small cell lung carcinoma. Cancer, 98, 1008-3.   DOI
10 Fukui T, Nonomura N, Tokizane T, et al (2001). Clinical evaluation of human telomerase catalytic subunit in bladder washings from patients with bladder cancer, Mol Urol, 5, 19-3.   DOI
11 Greider CW, Blackburn EH (1985). Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell, 43, 405-3.   DOI
12 Greider CW (1991). Telomeres. Curr Opin Cell Biol, 3, 444-1.   DOI
13 Hiyama E, Gollahon L, Kataoka T, et al (1996). Telomerase activity in human breast tumors. J Natl Cancer Inst, 17, 116-2.
14 Hiyama E, Hiyama K (2003). Telomerase as tumor marker. Cancer Lett, 194, 221-3.   DOI
15 Hsing AW, Gao YT, Han TQ, et al (2007). Gallstones and the risk of biliary tract cancer: a population-based study in China. Br J Cancer, 97, 1577-2.   DOI
16 Kim K H, Kaminker P, Campisi J (2002). Telomerase, aging and cancer: in search of a happy ending. Oncogene, 21, 503-1.   DOI
17 Ito H, Kyo S, Kanaya T, et al (1998). Detection of human telomerase reverse transcriptase messenger RNA in voided urine samples as a useful diagnostic tool for bladder cancer, Clin Cancer Res, 4, 2807-10.
18 Kapoor V K, McMichael AJ (2003). Gallbladder cancer: An 'Indian' disease. National Med J India, 16, 209-2.
19 Kawahara R, Odo M, Kinoshita H, (2007). Analysis of hTERT mRNA expression in biliary tract and pancreatic cancer. J Hepatobiliary Pancreat Surg, 14189-3.   DOI
20 Kyo S, Kanaya T, Takakura M, Tanaka M, Inoue, M (1999). Human telomerase reverse transcriptase as a critical determinant of telomerase activity in normal and malignant endometrial tissues. Int J Cancer, 80, 60-3.   DOI
21 Lerma E, Mora J (2005). Telomerase activity in "suspicious" thyroid cytology. Cancer Cancer Cytopathol, 105, 492-7.
22 Li W, Li L, Liu Z, et al (2008). Expression of the full-length telomerase reverse transcriptase (hTERT) transcript in both malignant and normal gastric tissues. Cancer Lett, 260, 28-6.   DOI
23 Luzar B, Poljak M, Cor A, Klopcic U, Ferlan-Marolt V (2005). Expression of human telomerase catalytic protein in gallbladder carcinogenesis. J Clin Pathol, 58, 820-5.   DOI
24 Malik A Imitiaz (2004). Gall bladder cancer: current status. Expert Opin Pharmacother, 5, 1271-7.   DOI
25 Miura N, Nakamura H, Sato R, et al (2006). Clinical usefulness of serum telomerase reverse transcriptase (hTERT) mRNA and epidermal growth factor receptor (EGFR) mRNA as a novel tumor marker for lung cancer. Cancer Sci, 97, 1366-3.   DOI
26 NCRP:Consolidated Report on Population Based Cancer Registries (2011-2012). National Cancer Registry Programme (Indian Council of Medical Research), Bangalore.
27 Miura N, Maeda Y, Kanbe T, et al (2005). Serum human telomerase reverse transcriptase messenger RNA as a novel tumor marker for hepatocellular carcinoma. Clin Cancer Res, 11, 3205-9.   DOI
28 NakayamaJ, TaharaH, Tahara E, et al (1998). Telomerase activation by hTERT in human normal fibroblasts and hepatocellular carcinomas. Nat Genet, 18, 65-68.   DOI
29 Nandakumar A, Gupta PC, Gangadharan P, Visweswara RN, Parkin DM (2005). Geographic pathology revisited: development of an atlas of cancer in India. Int J Cancer, 116, 740-4.   DOI
30 Hamdani NH, Qadri SK, AggarwallaRB, et al (2012). Clinicopathological study of gall bladder carcinoma with special reference to gallstones: our 8-year experience from Eastern India. Asian Pac J Cancer Prev, 13, 5613-7   DOI   ScienceOn
31 O'Sullivan JN, Bronner MP, Brentnall TA, et al (2002). Chromosomal instability in ulcerative colitisis related to telomere shortening. NatGenet, 32, 280-4.
32 Ohuchida K, Mizumoto K, Ogura Y, et al (2005). Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger rna levels in pancreatic juice samples for the diagnosis of pancreatic cancer. Clin Cancer Res, 11, 2285-2.   DOI
33 Poole J C, Andrews LG.Tollefsbol TO (2001). Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT). Gene, 269, 1-12.   DOI
34 Randi G, Franceschi S, La Vecchia C (2006). Gallbladder cancer worldwide: geographical distribution and risk factors. Int J Cancer, 118, 1591-2.   DOI
35 Shukla VK, Chauhan VS, Kumar M (2006). Telomerase activation - one step on the road to carcinoma of the gall bladder. Anticancer Res, 26, 4761-6.
36 Ryhu M (1995). Telomeres, telomerase, immortality. J Natl Cancer Inst, 87, 885-4.
37 Sanjeev KM, Tewari M, Kumar M, MK, Shukla HS (2011). Expression pattern of tumor endothelial marker 8 protein in gallbladder carcinomas. Asian Pac J Cancer Prev, 12, 507-2
38 Shay JW(1997). Telomerase in human development and cancer. J Cell Physiol, 173, 266-70.   DOI
39 Takakura M, Kyo S, Kanaya T, et al (1998). Expression of human telomerase subunits and correlation with telomerase activity in cervical cancer. Cancer Res, 58, 1558-1.
40 Tazuma S, Kajiyama G (2001). Carcinogenesis of malignant lesions of the gall bladder. The impact of chronic inflammation and gallstones. Langenbecks Arch Surg, 386, 224-9.   DOI
41 Uchida N, Tsutsui K, Ezaki T, Fukuma H, Kobara H, et al (2003). Combination of assay human telomerase reverse transcriptase mRNA and cytology using bile obtained by endoscopic transpapillary catheterization into the gallbladder for diagnosis of gallbladder carcinoma. Am J Gastroenterol, 98, 2415-9.   DOI
42 Koyanagi Y, Kobayashi D, Yajima T et al (2000). Telomerase activity is down regulated via decreases in hTERT mRNA but not TEP1 mRNA or hTERC during the differentiation of leukemic cells, Anticancer Res, 20, 773-8
43 Zou S Zhang (2000). Relative risk factors analysis of 3922 cases of gall bladder cancer. Zhonghau Wai Ke Zhi, 11, 805-8.