• Reproductive and Developmental Biology
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• 제35권1호
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• pp.17-22
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• 2011

This study was designed to determine the effect of electric field strength, duration and fusion buffer in fusion parameters on the rate of membrane fusion between the somatic cell and cytoplast for Korean cattle (HanWoo) somatic cell nuclear transfer (SCNT) procedure. Following electrofusion, effect of 5 or $10\;{\mu}M$ $Ca^{2+}$-ionophore of activation treatment on subsequent development was also evaluated. Cell fusion rates were significantly increased from 23.1% at 20 V/mm to 59.7% at 26 V/mm and 52.9% at 27 V/mm (p<0.05). Due to higher cytoplasmic membrane rupture or cellular lysis, overall efficiency was decreased when the strength was increased to 30 V/mm (18.5%) and 40 V/mm (6.3%) and the fusion rate was also decreased when the strength was at 25 V/mm or below. The optimal duration of electric stimulation was significantly higher in $25\;{\mu}s$ than 20 and $30\;{\mu}s$ (18.5% versus 9.3% and 6.3%, respectively, p<0.05). Two nonelectrolyte fusion buffers, Zimmermann's (0.28 M sucrose) and 0.28 M mannitol solution for cell fusion, were used for donor cell and ooplast fusion and the fusion rate was significantly higher in Zimmermann's cell fusion buffer than in 0.28 M mannitol (91.1% versus 48.4%, respectively, p<0.05). The cleavage and blastocyst formation rates of SCNT bovine embryos activated by $5\;{\mu}M$ $Ca^{2+}$-ionophore was significantly higher than the rates of the embryos activated with $10\;{\mu}M$ of $Ca^{2+}$-ionophore (70.0% versus 42.9% and 22.5% versus 14.3%, respectively; p<0.05). This result is the reverse to that of parthenotes which shows significantly higher cleavage and blastocyst rates in $10\;{\mu}M$ $Ca^{2+}$-ionophore than $5\;{\mu}M$ counterpart (65.6% versus 40.3% and 19.5% versus 9.7%, respectively; p<0.05). In conclusion, SCNT couplet fusion by single pulse of 26 V/mm for $25\;{\mu}s$ in Zimmermann's fusion buffer followed by artificial activation with $5\;{\mu}M$ $Ca^{2+}$-ionophore are suggested as optimal fusion and activation methods in Korean cattle SCNT protocol.

• Journal of Korean Neurosurgical Society
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• 제48권5호
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• pp.412-418
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• 2010

Objective : To evaluate the clinical and radiological outcomes of lumbar interbody fusion and its correlation with various factors (e.g., age, comorbidities, fusion level, bone quality) in patients over and under 65 years of age who underwent lumbar fusion surgery for degenerative lumbar disease. Methods : One-hundred-thirty-three patients with lumbar degenerative disease underwent lumbar fusion surgery between June 2006 and June 2007 and were followed for more than one year. Forty-eight (361%) were older than 65 years of age (group A) and 85 (63.9%) were under 65 years of age (group B). Diagnosis, comorbidities, length of hospital stay, and perioperative complications were recorded. The analysis of clinical outcomes was based on the visual analogue scale (VAS). Radiological results were evaluated using plain radiographs. Clinical outcomes, radiological outcomes, length of hospital stay, and complication rates were analyzed in relation to lumbar fusion level, the number of comorbidities, bone mineral density (BMD), and age. Results : The mean age of the patients was 61.2 years (range, 33-86 years) and the mean BMD was -2.2 (range, -4.8 to -2.8). The mean length of hospital stay was 15.0 days (range, 5-60 days) and the mean follow-up was 23.0 months (range, 18-30 months). Eighty-five (64.0%) patients had more than one preoperative comorbidities. Perioperative complications occurred in 27 of 133 patients (20.3%). The incidence of overall complication was 22.9% in group A, and 18.8% in group B but there was no statistical difference between the two groups. The mean VAS scores for the back and leg were significantly decreased in both groups (p < 0.05), and bony fusion was achieved in 125 of 133 patients (94.0%). There was no significant difference in bony union rates between groups A and B (91.7% in group A vs. 95.3% in group B, p = 0.398). In group A. perioperative complications were more common with the increase in fusion level (p = 0.027). Perioperative complications in both groups A (p = 0.035) and B (p = 0.044) increased with an increasing number of comorbidities. Conclusion : Elderly patients with comorbidities are at a high risk for complications and adverse outcomes after lumbar spine surgery. In our study, clinical outcomes, fusion rates, and perioperative complication rates in older patients were comparable with those in younger populations. The number of comorbidities and the extent of fusion level were significant factors in predicting the occurrence of postoperative complications. However, proper perioperative general supportive care with a thorough fusion strategy during the operation could improve the overall postoperative outcomes in lumbar fusion surgery for elderly patients.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.81-89
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• 2004

Tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and lymphotoxin-$\alpha$ (LT-$\alpha$, TNF-$\beta$) can initiate and perpetuate human diseases such as multiple sclerosis (MS), rheumatoid arthritis (RA), and insulin-dependent diabetes mellitus (IDDM). TNFs can be blocked by the use of soluble TNF receptors. However, since monomeric soluble receptors generally exhibit low affinity or function as agonists, the use of monomeric soluble receptors has been limited in the case of cytokines such as TNF-$\alpha$, TNF-$\alpha$, interleukin (IL)-1, IL-4, IL-6, and IL-13, which have adapted to a multi component receptor system. For these reasons, very high-affinity inhibitors were created for the purpose of a TNFs antagonist to bind the TNFR and trigger cellular signal by using the multistep polymerase chain reaction method. First, recombinant simple TNFR-Ig fusion proteins were constructed from the cDNA sequences encoding the extracellular domain of the human p55 TNFR (CD120a) and the human p75 TNFR (CD120b), which were linked to hinge and constant regions of human $IgG_1$ heavy chain, respectively using complementary primers (CP) encoding the complementary sequences. Then, concatameric TNFR-Ig fusion proteins were constructed using recombinant PCR and a complementary primer base of recombinant simple TNFR-Ig fusion proteins. For high level expression of recombinant fusion proteins, Chinese hamster ovary (CHO) cells were used with a retroviral expression system. The transfected cells produced the simple concatameric TNFR-Ig fusion proteins capable of binding TNF and inactivating it. These soluble versions of simple concantameric TNFR-Ig fusion proteins gave rise to multiple forms such as simple dimers and concatameric homodimers. Simple TNFR-1g fusion proteins were shown to have much more reduced TNF inhibitory activity than concatameric TNFR-Ig fusion proteins. Concatameric TNFR-Ig fusion proteins showed higher affinity than simple TNFR-Ig fusion proteins in a receptor inhibitor binding assay (RIBA). Additionally, concatameric TNFR-Ig fusion proteins were shown to have a progressive effect as a TNF inhibitor compared to the simple TNFR-Ig fusion proteins and conventional TNFR-Fc in cytotoxicity assays, and showed the same results for collagen induced arthritis (CIA) in mice in vivo.

• 한국진공학회지
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• 제16권5호
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• pp.388-396
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• 2007

KSTAR (Korea Superconducting Tokamak Advanced Research) 전류인입선(CL; Current Lead)은 4.5 K의 저온에서 운전되는 초전도 버스라인과 300 K의 실온에서 운전되는 MPS (Magnet Power Supply)를 전기적으로 연결하는 장치이다. 초기 플라즈마 발생시험을 위하여 TF (Toroidal Field) 및 PF (Poloidal Field) 리드박스에 전류인입선이 설치된다. TF 자석용 CL은 17.5 kA급 4 개의 CL에 최대 35 kA의 DC 전류가 인가되며, PF 자석용은 13 kA급 14 개의 CL에 350초간 $20\;{\sim}\;26\;kA$의 펄스 전류가 인가된다. 각각의 전류인입선은 TF 및 PF 자석에 전류를 인가하기 위한 버스라인이 연결되어 있으며, 전류인입선을 통해 초전도 버스라인으로 전달되는 전도열 및 전류인가시 발생되는 주울(Joule) 열을 차단하기 위한 헬륨냉매 제어시스템이 KSTAR 주장치와는 별도로 설치되어 있다. 리드박스 내 외부의 배관 및 제어시스템 설치완료 후 고진공 배기, 헬륨 누설검사, 전류인입선 유량 검사 및 액체질소 냉각시험을 실시하여 장치의 성능검증을 완료하였다.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2004년도 춘계학술발표회논문집
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• pp.383-388
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• 2004

The fusion of a different kind sensor is fusion of the obtained data by the respective independent technology. This is a important technology for the construction of 3D spatial information. particularly, information is variously realized by the fusion of LiDAR and mobile scanning system and digital map, fusion of LiDAR data and high resolution, LiDAR etc. This study is to generate union DEM and digital ortho image by the fusion of LiDAR data and high resolution image and monitor precisely topology, building, trees etc in urban areas using the union DEM and digital ortho image. using only the LiDAR data has some problems because it needs manual linearization and subjective reconstruction.

• Bulletin of the Korean Chemical Society
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• 제31권3호
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• pp.742-745
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• 2010

• Journal of Plant Biotechnology
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• 제41권2호
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• pp.65-72
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• 2014

Plant cells from which the cell walls have been enzymatically or mechanically removed are called protoplasts. The protoplasts are theoretically totipotent and can be used as sources of somatic cell fusion in practical breeding programs. Wild Solanum species have often been used as sources of important agricultural traits including diverse disease resistance. However, they cannot often be directly applied to breeding programs due to their sexual incompatibility with S. tuberosum. Somatic hybridization via protoplast fusion is one of the ideal methods to overcome this limitation and to introgress certain traits into S. tuberosum. This technique has still widely been used in potato since the first fusion was reported in 1970s. Therefore, this review highlights general perspectives of protoplast fusion and discusses the application of protoplast fusion in potato breeding.

• 센서학회지
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• 제26권1호
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• pp.1-6
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• 2017

The advanced driver assistant system (ADAS) requires the collection of a large amount of information including road conditions, environment, vehicle status, condition of the driver, and other useful data. In this regard, large-scale sensor networks can be an appropriate solution since they have been designed for this purpose. Recent advances in sensor network technology have enabled the management and monitoring of large-scale tasks such as the monitoring of road surface temperature on a highway. In this paper, we consider the estimation and fusion problems of the large-scale sensor networks used in the ADAS. Hierarchical fusion architecture is proposed for an arbitrary topology of the large-scale sensor network. A robust cluster estimator is proposed to achieve robustness of the network against outliers or failure of sensors. Lastly, a robust hierarchical data fusion scheme is proposed for the communication channel between the clusters and fusion center, considering the non-Gaussian channel noise, which is typical in communication systems.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제4권1호
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• pp.63-69
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• 2004

In this paper, we propose a general framework for sensor data fusion applied to control systems. Since many kinds of disturbances are introduced to a control system, it is necessary to rely on multisensor data fusion to improve control performance in spite of the disturbances. Multisensor data fusion for a control system is considered a sequence of making decisions for a combination of sensor data to make a proper control input in uncertain conditions of disturbance effects on sensors. The proposed method is applied to a typical control system of a flexible link system in which reduction of oscillation is obtained using a photo sensor at the tip of the link. But the control performance depends heavily on the environmental light conditions. To overcome the light disturbance difficulties, an accelerometer is used in addition to the existing photo sensor. Improvement of control performance is possible by utilizing multisensor data fusion for various output responses to show the feasibility of the proposed method in this paper.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제10권4호
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• pp.281-286
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• 2010

We propose a space and time based sensor fusion method and a robust landmark detecting algorithm based on sensor fusion for mobile robot navigation. To fully utilize the information from the sensors, first, this paper proposes a new sensor-fusion technique where the data sets for the previous moments are properly transformed and fused into the current data sets to enable an accurate measurement. Exploration of an unknown environment is an important task for the new generation of mobile robots. The mobile robots may navigate by means of a number of monitoring systems such as the sonar-sensing system or the visual-sensing system. The newly proposed, STSF (Space and Time Sensor Fusion) scheme is applied to landmark recognition for mobile robot navigation in an unstructured environment as well as structured environment, and the experimental results demonstrate the performances of the landmark recognition.