Browse > Article
http://dx.doi.org/10.5010/JPB.2014.41.2.65

Potato breeding via protoplast fusion  

Cho, Kwang-Soo (Highland of Agriculture Research Center, National Institute of Crop Science, RDA)
Park, Tae-Ho (Department of Horticulture and Institute of Life and Environment, Daegu University)
Publication Information
Journal of Plant Biotechnology / v.41, no.2, 2014 , pp. 65-72 More about this Journal
Abstract
Plant cells from which the cell walls have been enzymatically or mechanically removed are called protoplasts. The protoplasts are theoretically totipotent and can be used as sources of somatic cell fusion in practical breeding programs. Wild Solanum species have often been used as sources of important agricultural traits including diverse disease resistance. However, they cannot often be directly applied to breeding programs due to their sexual incompatibility with S. tuberosum. Somatic hybridization via protoplast fusion is one of the ideal methods to overcome this limitation and to introgress certain traits into S. tuberosum. This technique has still widely been used in potato since the first fusion was reported in 1970s. Therefore, this review highlights general perspectives of protoplast fusion and discusses the application of protoplast fusion in potato breeding.
Keywords
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Ahn YK, Kim HY, Choi, HS, Kim K-T, Park HG (2001a) Production of interspecific somatic hybrids between the cultivated potato (Solanum tuberosum) and the wild species (S. brevidens). J Korean Soc Hort Sci 24:420-424
2 Ahn YK, Kang JC, Kim HY, Lee SD, Park HG (2001b) Resistance to blackleg and tuber soft rot in interspecific somatic hybrids between S. brevidens and S. tuberosum (‘Superior’, ‘Dejima, and dihaploid of ‘Superior’). J Korean Soc Hort Sci 24: 430-434
3 Ahn Y-K, Ghislain M, Choi H-S, Park H-G (2002) Selection of somatic hybrids of potato using molecular marker and chloroplast number. Korean J Breed 34:188-194
4 Ahn YK, Park T-H (2013) Resistance to common scab developed by somatic hybrids between Solanum brevidens and Solanum tuberosum. Acta Agric Scand Sect B-Soil & Plant Sci 63: 595-603
5 Cai X, Liu J, Xie C (2004) Mesophyll protoplast fusion of Solanum tuberosum and Solanum chacoense and their somatic hybrid analysis. Acta Hort Sinica 31:623-626
6 Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R (2007) Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plstome and improved tolerance to salinity. Plant Cell Tiss Organ Cult 91: 179-189   DOI
7 Binding H, Nehls R, Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones of Solanum tuberosum L. Physiol Plant 43:52-54   DOI
8 Carlson PS, Smith HH, Dearing RD (1972) Parasexual interspecific plant hybridization. Proc Natl Acad Sci USA 69:2292-2294   DOI   ScienceOn
9 Cardi T, Amborsio FD, Consoli D, Puite KJ, Ramulu KS (1993) Production of somatic hybrids between frost-tolerant Solanum commersonii and S. tuberosum: characterization of hybrid plants. Theor Appl Genet 87:193-200
10 Chase SS (1963) Analytical breeding scheme in Solanum tuberosum L.- A scheme utilizing parthenotes and other diploid stocks. Can J Genet Cytol 5:359-363   DOI
11 Chen Q, Li HY, Shi YZ, Beasley D, Bizimungu B, Goettel MS (2008) Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools. Can J Plant Sci 88:611-619   DOI   ScienceOn
12 Chen L, Guo X, Xie C, He L, Cai X, Tian L, Song B, Liu J (2013) Nuclear and cytoplasmic genome components of Solanum tuberosum + S. chacoense somatic hybrids and three SSR alleles related to bacterial wilt resistance. Theor Appl Genet 126:1861-1872   DOI   ScienceOn
13 Espejo R, Cipriani G, Rosel G, Golmirzaie A, Roca W (2008) Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L. subsp. tuberosum and the wild species Solanum circaeifolium Bitter. Rev Peru Biol 15:73-78
14 Cho HM, Kim-Lee HY, Om YH, Kim JK (1997) Influence of endosperm balance number (EBN) in interploidal and interspecific crosses between Solanum tuberosum dihaploids and wild species. Korean J Breed 29:154-161
15 Eeckhaut T, van Laere K, de Riek J, van Huylenbroeck J (2006) Overcoming interspecific barriers in ornamental plant breeding. In: Teixeira da Silva J (ed), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Global Science Books, London, pp 540-551
16 Eeckhaut T, Lakshmanan PS, Deryckere D, van Bockstaele E, van Huylenbroeck J (2013) Progress in plant protoplast research. Planta 238:991-1003   DOI   ScienceOn
17 Fock I, Collonnier C, Lavergne D, Vaniet S, Ambroise A, Luisetti J, Kodja H, Sihachakr D (2007) Evaluation of somatic hybrids of potato with Solanum stenotomum after a long-term in vitro conservation. Plant Physiol Biochem 45:209-215   DOI   ScienceOn
18 Frei U, Stattmann M, Lőssel and Wenzel G (1998) Aspects of fusion combining ability of dihaploid Solanum tuberosum L.: Influence of the cytoplasm. Potato Res 41:155-162   DOI
19 Gamborg OL (2002) Plant tissue culture. Biotechnology. Milestones. In Vitro Cell Dev Biol Plant 38:84-92   DOI   ScienceOn
20 Gancle A, Grimplet J, Sauvage F, Ollitrault P, Brillouet J (2006) Pre-dominant expression of diploid mandarin leaf proteome in two citrus mandarin-derived somatic allotetraploid hybrids. J Agr Food Chem 54:6212-6218   DOI   ScienceOn
21 Greplová M, Polzerová, Vlastníková H (2008) Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum. Acta Physiol Plant 30:787-796   DOI
22 Jiang L, Cai Y, Xia G, Xiang F (2012) Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization. Protoplasma 249:737-745   DOI   ScienceOn
23 Helgeson JP, Kaberlach GT (1999) Somatic hybrids of Solanum tuberosum and related species. In: Altman A, Ziv M, Izhar S (eds), Plant biotechnology and in vitro biology in the 21st century, Kluwer, Dordrecht, pp 151-154
24 Iovene M, Aversano R, Savarese S, Caruso I, Di Matteo A, Cardi T, Frusciante L, Carputo D (2012) Interspecific somatic hybrids between Solanum bulbocastanum and S. tuberosum and their haploidization for potato breeding. Bio Plant 56: 1-8   DOI   ScienceOn
25 Jansky S (2000) Breeding for disease resistance in potato. In: Janick J (ed), Plant breeding reviews, Wiley, New York, pp 69-155
26 Kim SR, Ahn YK, Kim TG, Kang HS, Song SW, Kim BC, Kang SG (2013) Breeding of a new cultivar ‘Jeseo’ with resistance to common scab. Kor J Breed Sci 45:468-473   DOI
27 Kim-Lee H, Moon JS, Hong YJ, Kim MS, Cho HM (2005) Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. Amer J of Potato Res 82:129-137   DOI   ScienceOn
28 Lightbourn GJ, Veilleux RE (2007) Production and evaluation of somatic hybrids derived from monoploid potato. Amer J of Potato Res 84:425-435   DOI
29 Lakshmanan PS, Eeckhaut T, Deryckere D, van Bockstaele E, van Huylenbroeck J (2013) Asymmetric somatic plant hybridization: Status and applications. Amer J Plant Sci 4:1-10
30 Lian Y, Lin G, Zhao X, Lim H (2011) Production and genetic characterization of somatic hybrids between leaf mustard (Brassica juncea) and broccoli (Brassica oleracea). In Vitro Cell Dev Plant 47:289-296   DOI   ScienceOn
31 Liu J, Xu X, Deng X (2005) Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tiss Org Cult 82:19-44   DOI
32 Olivares-Fuster O, Duran-Vila N, Navarro L (2005) Electrochemical protoplast fusion in Citrus. Plant Cell Rep 24:112-119   DOI   ScienceOn
33 Liu C, Li S, Wang M, Xia G (2012) A transcriptomic analysis reveals the nature of salinity tolerance of a wheat introgression line. Plant Mol Biol 78:159-169   DOI   ScienceOn
34 Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Comm 43:203-218   DOI
35 Nouri-Ellouz O, Gargouri-Bouzid R, Sihachakr D, Triki MA, Ducreux G, Drira N, Lakhoua L (2006) Production of potato intraspecific somatic hybrids with improved tolerance to PVY and Pythium aphanidermatum. J Plant Physiol 163:1321-1332   DOI   ScienceOn
36 Ortiz R, Ehlenfeldt MK (1992) The importance of endosperm balance number in potato breeding and the evolution of tuber-bearing Solanum species. Euphytica 1992:105-113
37 Razdan M (2003) Somatic hybridization. In: Razdan M (ed), Introduction to plant tissue culture, Science Publishers, Enfield, pp 147-175
38 Patel D, Power J, Anthony P, Badakshi F, Heslop-Harrison J, Davey M (2011) Somatic hybrid plants of Nicotiana x sanderae + N. debneyi with fungal resistance to Peronospora tabacina. Ann Bot 108:809-819   DOI   ScienceOn
39 Polzerova H, Patzak J, Greplová M (2011) Early characterization of somatic hybrids from symmetric protoplast electrofusion of Solanum pinnatisectum Dun. and Solanum tuberosum L. Plant Cell Tiss Organ Cult 104:163-170   DOI
40 Przetakiewicz J, Nadolska-Orczyk A, Kuć D, Orczyk W (2007) Tetraploid somatic hybrids of potato (Solanum tuberosum L.) obtained from diploid breeding lines. Cell Mol Biol Lett 12: 253-267   DOI   ScienceOn
41 Rokka V-M (2009) Potato haploids and breeding. In: Touraev A, Forster BP, Jain SM (eds), Advances in haploid production in higher plants, Springer Science + Business Media B.V., Dordrecht, pp 199-208
42 Shi YZ, Chen Q, Li HY, Beasley D, Lynch DR (2006) Somatic hybridization between Solanum tuberosum and S. cardiophyllum. Can J Plant Sci 86:539-545   DOI   ScienceOn
43 Rokka V-M, Laurila J, Tauriainen A, Laakso I, Larkka J, Metzler M, Pietilä (2005) Glycoalkaloid aglycone accumulations associated with infection by Clavibacter michiganensis ssp. sepedonicus in potato species Solanum acaule and Solanum tuberosum and their interspecific somatic hybrids. Plant Cell Rep 23:683-691   DOI
44 Sarkar D, Tiwari J, Sharma S, Sharma Poonam S, Gopal J, Singh B, Luthra S, Pandey S, Pattanayak D (2011) Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun. Plant Cell Tiss Org Cult 107:427-440   DOI
45 Tek AL, Stevenson WR, Helgeson JP, Jiang J (2004) Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato. Theor Appl Genet 109: 249-254
46 Smyda P, Jakuczun H, Dębski K, Śliwka J, Thieme R, Nachtigall M, Wasilewicz-Flis I, Zimnoch-Guzowska E (2013) Development of somatic hybrids Solanum x michoacanum Bitter. (Rydb.) (+) S. tuberosum L. and autofused 4x S. x michoacanum plants as potential sources of late blight resistance for potato breeding. Plant Cell Rep 32:1231-1241   DOI   ScienceOn
47 Szczerbakowa A, Tarwacka J, Oskiera M, Jakuczun H, Wielgat B (2010) Somatic hybridization between the diploids of S. x michoacanum and S. tuberosum. Acta Physiol Plant 32: 867-873   DOI
48 Tarwacka J, Polkowska-Kowalczyk L, Kolano B, Śliwka J, Wielgat B (2013) Interspecific somatic hybrids Solanum villosum (+) S. tuberosum, resistance to Phytophthora infestans. J Plant Physiol 170:1541-1548   DOI   ScienceOn
49 The Potato Genome Sequencing Consortium (2011) Genome sequence and analysis of the tuber crop potato. Nature 475: 189-197   DOI   ScienceOn
50 Trabelsi S, Gargouri-Bouzid R, Vedel F, Nato A, Lakhoua L, Drira N (2005) Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Plant Cell Tiss Org Cult 83:1-11   DOI
51 Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T (2008) Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet 116:691-700   DOI
52 Wang GX, Tang Y, Yan H, Sheng XG, Hao WW, Zhang L, Lu K, Liu F (2011a) Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials. Plant Cell Reports 30:1811-1821   DOI   ScienceOn
53 Thieme R, Rakosy-Tican E, Nachtigall M, Schubert J, Hammann T, Antonova O, Gavrilenko T, Heimbach U, Thieme T (2010) Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. and two commercial potato cultivars. Plant Cell Rep 29:1187-1201   DOI
54 Tiwari JK, Poonam, Sarkar D, Pandey SK, Gopal J, Kumar SR (2010) Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tiss Organ Cult 103:175-187   DOI
55 Wang L, Pan Z, Guo W (2010) Proteomic anlaysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo. Plant Cell Tiss Org Cult 103:165-174   DOI
56 Wang J, Zhao C, Liu C, Xia G, Xiang F (2011b) Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization BioMedCentral Plant Biol 11:71   DOI   ScienceOn
57 Wang M, Peng Z, Hong S, Zhi D, Xia G (2012) Hybrid inflorescences derived from gamma-fusion of Arabidopsis thaliana with Bupleurum scorzonerifolium. Protoplasma 249: 197-205   DOI
58 Xia G (2009) Progress of chromosome engineering mediated by asymmetric somatic hybridization. J Genet Genomics 36: 547-556   DOI   ScienceOn
59 Xu XY, Hu ZY, Li JF, Liu JH, Deng XX (2007) Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis: Regeneration and characterization of hybrid shoots. Plant Cell Rep 26:1263-1273   DOI
60 Yu Y, Li Z, Wang P, Xiang F (2012a) Genetic and biochemical characterization of somatic hybrids between Bupleurum scorzonerifolium and Gentianopsis paludosa. Protoplasma 249:1029-1035   DOI   ScienceOn
61 Yu X, Chu B, Liu R, Sun J, Brian J, Wang H, Shuijin Z, Sun Y (2012b) Characteristics of fertile somatic hybrids of G. hirsutim L. and G. trilobum generated via protoplast fusion. Theor Appl Genet 125:1503-1516   DOI   ScienceOn
62 Yu Y, Ye W, He L, Cai X, Liu T, Liu J (2013) Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. Plant Cell Rep 32:1687-1701   DOI   ScienceOn
63 Wang X, Zhou J, Yang Y, Yu F, Chen J, Yu C, Wang F, Cheng Y, Yan C, Chen J (2013) Transcriptome analysis of a progeny of somatic hybrids of cultivated rice (Oryza sativa L.) and wild rice (Oryza meyeriana L.) with high resistance to bacterial blight. J Phyto Pathol 161:324-334
64 Boltowicz D, Szczerbakowa A, Wielgat B (2005) RAPD analysis of the interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum. Cell Mol Biol Lett 10:151-162
65 Shepard JP, Totten RE (1977) Mesophyll cell protoplasts of potato. Isolation, proliferation, and plant regeneration. Plant Physiol 60:313-316   DOI   ScienceOn