• Proceedings of the Korea Concrete Institute Conference
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• 2005.05b
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• pp.225-228
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• 2005

This paper was accomplished for analyzing the reason of the above deterioration happened on the deck of concrete bridge. The bridge was constructed at 660m above the sea level having more freezing and snowing days. Therefore, it is placed on the particular condition sprinkling $CaCl_2$ enough for keeping up with moderate traffic condition. When it is considered to the former condition, the bridge can be assumed to potentialities for combined deterioration with freezing-thawing under sprinkling deicing chemical. Core specimens were gathered from the concrete deck for clearing the reason of the above deterioration exactly, and it is used for various tests for measuring the compressive strength, elastic modulus, content of $Cl^-$, freezing-thawing at the fresh and salt water. As a result of freezing-thawing test, the specimen at the fresh water has over 90$\%$ of durability factor, but another specimen at 1$\%$ of salt water has 0$\%$ of durability factor at 140 cycles of the freezing-thawing. The result means that frost damage is sccelerated at the salt water. Therefore, the deterioration of the concrete deck is estimated to be occured by combined effects of freezing-thawing and chloride ion attack.

• Journal of Korean Society of Forest Science
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• v.103 no.4
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• pp.528-536
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• 2014

The aim of this study was to investigate the damage characteristics induced by winter freezing of evergreen broad-leaved trees distributed in urban area of warm temperate forest zone, which are gaining increased interest recently as climate change. The results of the study indicated that, as for 'stem injury', Camellia japonica Linne (59.6%) had the weakest damage, while Ternstroemia gymnanthera Sprague (83.3%) had the most severe damage. By the visual evaluation of freezing injury according to the characteristics of plantations environment, the observations of single planting (p<0.01) in planting method had weaker damage than those of group planting, the observations of under tree (p<0.001), plain (p<0.001), and organic matter plenty (p<0.05) in plantations characteristics had significant differences with relatively weaker damage than the observations of open space, slop, and scarcity, respectively. Tree height (-0.432) and crown width (-0.470) among growth factors were negatively correlated with the severity of damage, respectively. Therefore, the selection of tree species is vital for the successful creation of these urban forested area by making considerations of planting environment, and further research on evergreen broad-leaved trees is needed in this aspect.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.25-28
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• 2008

Generally, reinforced concrete structures exposed to the outside temperature are affected by freezing and thawing process during winter and early spring. These freezing and thawing process can lead to the reduction in durability of concrete as cracking or surface spalling. This paper is to study the hysteretic behavior of RC beams exposed to freezing and thawing under cyclic loadings. To compare the difference in hysteretic behavior of RC Beams, limited tests were conducted under different types of damage and freezing and thawing cycles. For this purpose, six specimens were tested. It is thought that experimental results will be used as basic data to evaluate hysteretic behavior of RC beams exposed to freezing and thawing.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1999.10c
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• pp.294-300
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• 1999

After researching the physical properties of the concrete included Rice Husk Ash(RHA concrete) and workability of fresh concrete admixed RHA, we have tested durability of RHA-concrete against freeaing and thawing in the winter using rapid freezing and thawing test method(KS F 2456) . There aretwo hypotheses to explain the failure mechanism of a freezing and thawing action. First, the hydraulic pressure in the pores of freezing concrete make an internal stress of concrete structures outbreaking micro crack in the face of concrete, Second, Frost action causing damage to cement paste repeatedly come from soil frost action, freezing water in the capillaries. Initial Relative Dynamic Modulus of Elasticity (DME) was biggest in cae of unit binder weight 600kgf/㎥ and relative dynamic modulus of elasticity increased until 300cycles. In general , initial relative DME was proportional to unit binder weight . Relative DME was decreased in proportion to unit binder weight in the case of 300, 400, 500kgf/㎥ , but relative DME fo the others remained more than 90% until 300 cycles. It was not good effect of intermixed RHA to concrete in case of below unit binder weight 300kgf/㎥ and the resistance of freezing and thawing was not good either.

• Journal of the Korean Ceramic Society
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• v.31 no.9
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• pp.949-956
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• 1994

In order to investigate the relationship between pore size distribution and freezing-thawing resistance of mortars, polymer-cement mortars were prepared by using styrene-butadiene rubber latex, ethylene-vinyl acetate emulsion and polyacrylic ester emulsion with various polymer-cement ratios at constant flow. From the results of the test, polymer-cement mortars had a good pore size distribution for freezing-thawing resistance compared with unmodified mortars because of having a small pore volume in the pore radius range of 103~104 $\AA$ affecting on the frost damage. And the freezing-thawing resistance of polymer-cement mortars was improved with increasing polymer-cement ratio.

• Clinical and Experimental Reproductive Medicine
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• v.50 no.4
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• pp.213-222
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• 2023

Cryopreservation is an option for the preservation of pre- or post-pubertal female or male fertility. This technique not only is beneficial for human clinical applications, but also plays a crucial role in the breeding of livestock and endangered species. Unfortunately, frozen germ cells, including oocytes, sperm, embryos, and spermatogonial stem cells, are subject to cryoinjury. As a result, various cryoprotective agents and freezing techniques have been developed to mitigate this damage. Despite extensive research aimed at reducing apoptotic cell death during freezing, a low survival rate and impaired cell function are still observed after freeze-thawing. In recent decades, several cell death pathways other than apoptosis have been identified. However, the relationship between these pathways and cryoinjury is not yet fully understood, although necroptosis and autophagy appear to be linked to cryoinjury. Therefore, gaining a deeper understanding of the molecular mechanisms of cryoinjury could aid in the development of new strategies to enhance the effectiveness of the freezing of reproductive tissues. In this review, we focus on the pathways through which cryoinjury leads to cell death and propose novel approaches to enhance freezing efficacy based on signaling molecules.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.3
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• pp.79-87
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• 2011

Concrete incorporating high volume blast-furnace slag placed in cold weather regions might be in danger of initial frost damage because dependently on the mix proportions, the setting and the hardening would be remarkably delayed. Therefore, this study investigated to effect of the degree of frost on the strength development and the resistance to freezing and thawing of the concrete incorporating blast-furnace slag when being subjected to freeze at early age. As the experimental results, the concrete incorporating blast-furnace slag attacked by initial frost damage showed the remarkable reduction of both the compressive strength development and the resistance to freezing and thawing. Especially, the resistance to freezing-thawing of the concrete incorporating high volume blast-furnace slag became much lower than that of the normal concrete.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• Proceedings of the Korea Concrete Institute Conference
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• 1996.04a
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• pp.48-51
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• 1996

Recently, frost damage of storage tank for admixture agent caused by low temperature in winter and quality deterioration of admixture agent ofen happened. But, many problems are caused because of no applicable regulations of admixture agent facility and so on. Therefore, this study presents the reference data about using and quality control of admixture agent in practice and the judgement data about working out a counter plan of suitable heat insulation of injecion equipment of admixture agent exposed at outside in winter by measuring the freezing point of admixture agent for concrete.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.7
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• pp.1055-1061
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• 2014

Innovative freezing technology is currently applied to preserve foodstuffs for long-term storage. Generally, the quality of frozen food is closely related to the types of freezing and thawing processes. In this study, we characterized the physicochemical properties of onions depending on freezing rate. When onions were frozen at $-40^{\circ}C$, freezing rates were 0.1, 0.5, and $0.7^{\circ}C/min$ depending on air-blast quick freezer mode. Onions were thawed by microwave irradiation at 400 W. Hardness of onion dramatically decreased after freezing and thawing compared with blanched onion. However, the fastest freezing rate did not affect hardness. Thawing loss of onion decreased with a faster freezing rate. For morphological observation, onion frozen at a faster rate showed a smaller ice-crystal size. Vitamin C content decreased upon blanching or freezing, but there was no significant difference according to freezing rate. Although free sugar content also decreased upon blanching and freezing, its highest content was at $0.7^{\circ}C/min$ freezing. Among organic acids, malic acid content was highest at $0.7^{\circ}C/min$ freezing. Based on this study, it could be suggested that a faster freezing rate is effective to improve frozen food quality in accordance with preventing tissue damage or minimizing destruction of nutrients.