• Asian-Australasian Journal of Animal Sciences
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• v.13 no.2
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• pp.188-191
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• 2000

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

• Journal of Elementary Mathematics Education in Korea
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• v.19 no.4
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• pp.563-588
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• 2015

In elementary school, didactical transposition is inevitable due to several reasons. In mathematics, addition and multiplication are taught as binary operations, subtraction and division are taught as unary operations. But in elementary school, we try to teach all the four operations as binary operations by didactical transposition. In 'Mastering' the concepts of the four operations, the way of concept introduction is dealt importantantly. So it is different from understanding the four operations. In this study, we analyzed the four operations of natural numbers and fractions from two perspectives: concept understanding (how to introduce concepts and how to choose an operation) and connection between the operations. As a result, following implications were obtained. In division of fractions, students attempted a connection with multiplication of fractions right away without choosing an operation, based on the situation. Also, to understand division of fractions itself, integrate division of fractions presented from the second semester of the fifth grade to the first semester of the sixth grade are needed. In addition, this result can be useful in the future textbook development.

• Korean journal of food and cookery science
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• v.14 no.5
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• pp.577-583
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• 1998

Methanol extracts of Commelina communis, a Korean wild edible plant showing hypoglycemic effect on diebetic rats were fractionated systematically by hexane, chloroform, butanol, and water, and these four fractions were administered orally to streptozotocin-induced diabetic rats to evaluate the hypoglycemic effect. The butanol and water fractions of Commelina communis lowered blood glucose levels of diebetic rats at a dose of 500 mg/kg/day. Administration of each fraction except that of chloroform increased the plasma protein levels. Liver protein levels were significantly higher in the butanol- and water fraction-fed groups. The results suggest that the butanol fraction of Commelina communis has hypoglycemic effect on diabetic rats and the intake of Commelina communis may be beneficial for the management of diabetes mellitus.

• Journal of Nutrition and Health
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• v.21 no.4
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• pp.260-267
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• 1988

Forteen healthy women, with normal serum cholesterol levels, were supplemented with two egg yolks daily for the 1st week and four egg yolks for the 2nd week to their customary diets for 2 weeks period. Serum cholesterol, triglyceride, phospholipid and lipoprotein fractions were determined. Serum cholesterol and phospholipid levels were not affected by the supplementation of egg yolks. However, triglyceride level was significantly increased by the supplementation of four egg yolks at the end of the 1st week, but the level remained normal range. The serum lipoprotein pattern was influenced by the supplementation of egg yolks : HDL fraction significantly decreased, while LDL and VLDL fractions significantly increased.

• YAKHAK HOEJI
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• v.27 no.3
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• pp.221-227
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• 1983

New lectins, lymphoagglutinating lectins from mung beans (MBLA) have been isolated and purified. Mung beans crude extracts were made with 0.15M NaCl and these were purified through anionic exchange chromatography. Four fractions were obtained from DEAE Sephadex A-50 by salt gradients elution. Lectin activity, enzyme activity, protein assay, identification of purity by polyacrylamide gel electrphoresis and immunochemical studies were carried out with these four fractions. Through these results, it can be suggested that 0.2M fraction is newly found potent MBLA. There were some relationships with MBLA and L-PHA but no similarities were observed between MBLA and E-PHA.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.227-232
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• 2000

Fresh pine needles were collected and extracted with 95% methanol and the extract was concentrated to determine its antimicrobial activity. The methanol extract had a considerable inhibitory effect on the tested bacteria, such as Esherichia coli 0157;H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus. The methanol extract of pine needles was further fractionated to chloroform, ethylacetate, butanal, and water fractions. Among these four fractions, the butanol and water fractions, which showed a relatively strong inhibitory effect on all of the tested bacteria, were purified and the minimum ingibitory concentration (MIC) was determined for each microorganism. The MIC raged between 25 mg/ml and 45 mg/ml depending on the microorganism. the purified active fractions were applied to sterilized milk as a model food system to define the antimicrobial effectiveness and it was found that the antimicrobial activities in the water fractions were stronger than those in the butanol fractions.

• Journal of Life Science
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• v.19 no.5
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• pp.607-614
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• 2009

This study was carried out to investigate the anticarcinogenic and antioxidative activities of Hemicentrotus pulacherrimus (HP). HP was extracted with methanol (HPM), which was then further fractionated into four sub-fractions by using the solvent partition method, affording methanol (HPMM), hexane (HPMH), butanol (HPMB) and aqueous (HPMA) soluble fractions. We determined the anticarcinogenic activities of these four fractions in four kinds of cancer cell lines, such as HepG2, HT29, MCF-7 and B16-F10, by MTT assay. Among various fractions from HPM, the HPMH showed the strongest growth inhibition effect. We also determined the inductive effect on quinone reductase (QR) of HP fractions. HPMB fraction exhibited strong inductive effects in HepG2 cells at a level of 90 ${\mu}g/ml$, showing inductive indexes of 2.26 compared to the control value of 1.0. The antioxidant activities of fractions from HP were also investigated by measuring the scavenging activities of HP against reactive oxygen speicies (ROS), peroxynitrite (ONOO-) and NO. Among the various solvent fractions, HPMH fractions displayed marked antioxidative activities.

• Journal of Ginseng Research
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• v.9 no.2
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• pp.204-212
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• 1985

Lipid and fatty acid compositions of the bound lipids in Panax quinquefolium (Korea, Japan and China), Panax quinquefolium (America, Canada) and Panax notoginseng (China) were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. The could lipid contents in various ginsengs were 0.29 to 0.48%, in which neutral lipid fractions were 63.6 to 67.3%, glycolipid fractions 21.9 to 25.7% and phospholipid fractions 7.7 to 12.4%. The content compositions of neutral lipid fractions were lower and those of glycolipid and phospholipid fractions were higher in the bound lipids than in the free lipids from the various ginseng. The major components were fatty acids, diglycerides and free sterols in neutral lipid fractions, monogalactosyl diglyceride, sterol glucoside and esterified steryl g1ycoside in glycolipid fractions and phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidic acid in phosphoipid fractions. Seventeen fatty acids were analyzed in the four bound lipid fractions from the various ginsengs and main fatty acids were linoleic acid, palmitic acid and oleic acid. Total saturated fatty acid and palmitic acid contents were higher and total unsaturated fatty acid and linoleic acid contents lower in the total bound lipids than in the total free lipids from the various ginsengs.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1249-1253
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• 2006

The fractions of Capsosiphon fulvescens were studied to verify the anticancer and immunostimulating activity. The fractions from the ethanol extract of C. fulvescens were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, methanol, butanol and H$_2$O. The cytotoxic effects of C. fulvescens fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2)and rat glioma cell line (C6) were investigated. Ethyl ether fraction of C. fulvescens showed the highest cytotoxicity against all four cell lines tested. In addition, H$_2$O fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of C. fulvescens fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of C. fulvescens extract stimulated NO production with concentration dependant manner. These results suggest that C. fulvescens may be a useful candidate for a natural antitumor and immune-stimulating agent.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.8
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• pp.1228-1233
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• 1999

A study was conducted on four maize cultivars to determine the dry matter and fibre digestibility as influenced by advancing plant age. Samples of maize cultivars Akbar, Neelum, UM-81 and IZ-31 were harvested at weekly intervals/ growth stages. The samples of morphological fractions such as leaf and stem were also collected at various growth stages. Whole mixed fodder and different fractions of maize plant were analysed for their chemical composition and in vitro digestibility. The results showed that in vitro dry matter digestibility (IVDMD) of whole maize plant, leaf and stem decreased significantly with advancing stage of maturity. Digestibility of NDF, ADF, hemicellulose and cellulose decreased significantly in all plant parts with advancing plant age/growth stages. Maximum values for the digestibility of dry matter and various cell wall constituents were observed in leaf, followed by whole plant and stem fractions. Cultivars were observed to have significant effect of IVDMD and digestibility of NDF, ADF and cellulose in all plant fractions. The results indicated that digestibility of maize fodder was affected by stage of maturity and cultivars. However, maturity had a greater effect on digestibility in all plant fractions than did cultivars. Dry matter contents were found to be significantly and negatively correlated with IVDMD of whole plant and its leaf and stem fractions. Based on correlations, regression equations were computed to predict IVDMD.