• Polymer(Korea)
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• v.36 no.2
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• pp.177-181
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• 2012

This study presents the preparation of double emulsions in a poly(dimethylsiloxane) (PDMS)-based microfluidic device. To improve the wettability of hydrophilic continuous phase onto a hydrophobic PDMS microchannel, the surface was modified with 3-(trimethoxysilyl) propyl methacrylate (TPM) and then sequentially reacted with acrylic acid monomer solution, which produced selective covalent bonding between acrylic acids and methacrylate groups. For the proof of selective surface modification, tolonium chloride solution was used to identify the modified region and we confirmed that the approach was successfully performed. When water containing 0.5% w/w sodium dodecyl sulfate and 1% w/w Span80 with hexadecane were loaded into the selectively modified microfluidic channels, we can produce stable double emulsion. Based on the spreading coefficients, we predict the morphology of double emulsions. Our proposed method efficiently produces monodisperse double emulsions having 48.5 ${\mu}m$(CV:1.6%) core and 65.1 ${\mu}m$ (CV:1.6%) shell. Furthermore, the multiple emulsions having different numbers of core were easily prepared by simple control of flow rates.

• Journal of Korea Water Resources Association
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• v.36 no.2
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• pp.285-300
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• 2003

An event-based, kinematic, infiltration-excess, and distributed rainfall-runoff model using weather radar and Geographic Information System(GIS) was developed to acknowledge and account lot the spatial variability and uncertainty of several parameters relevant to storm surface runoff and surface flow The developed model is compatible with raster GIS and spatially and temporally varied rainfall data. To calibrate the model, Monte Carlo simulation and a likelihood measure are utilized; allowing for a range of possible system responses from the calibrated model. Using rain gauge adjusted radar-rainfall estimates, the developed model was applied and evaluated to a limited number of historical events for the Ralston Creek and Goldsmith Gulch basins within the Denver Urban Drainage and Flood Control District (UDFCD) that contain mixed land use classifications. While based on a limited number of Monte Carlo simulations and considered flood events, Nash and Sutcliffe efficiency score ranges of -0.19∼0.95 / -0.75∼0.81 were obtained from the calibrated models for the Ralston Creek and Goldsmith Gulch basins, based on a comparison of observed and simulated hydrographs. For the Ralston Creek and Goldsmith Gulch basins, Nash and Sutcliffe efficiency scores of 0.88/0.10, 0.14/0.71, and 0.99/0.95 for runoff volume, peak discharge, and time to peak, respectively, were obtained from the model.

• Journal of Life Science
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• v.16 no.4
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• pp.589-597
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• 2006

Genistein, a natural isoflavonoid phytoestrogen, is a strong inhibitor of protein tyrosine kinase and DNA topoisomerase activities. There are several studies documenting molecular alterations leading to cell cycle arrest and induction of apoptosis by genistein as a chemopreventive agent in a variety of cancer cell lines; however, its mechanism of action and its molecular targets on human bladder carcinoma and leukemic cells remain unclear. In the present study, we have addressed the mechanism of action by which genistein suppressed the proliferation of T24 bladder carcinoma and U937 leukemic cells. Genistein significantly inhibited the cell growth and induced morphological changes, and induced the G2/M arrest of the cell cycle in both T24 and U937 cells with a relatively stronger cytotoxicity in U937. The G2/M arrest in T24 cells was associated with the inhibition of cyclin A, cyclin B1 and Cdc25C protein expression without alteration of tumor suppressor p53 and cyclin-dependent kinase (Cdk) inhibitor p21(WAF1/CIP1). However, the inhibitory effects of genistein on the cell growth of U937 cells were connected with a marked inhibition of cyclin B1 and an induction of Cdk inhibitor p21 proteins by p53-independent manner. These data suggest that genistein may exert a strong anticancer effect and additional studies will be needed to evaluate the different mechanisms between T24 and U937 cells.

• Molecules and Cells
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• v.40 no.3
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• pp.222-229
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• 2017

Adipose-derived stem cells (ADSCs) were previously considered to have an anti-inflammatory effect, and Interleukin-$1{\beta}$ ($IL-1{\beta}$) was found to be a pro-inflammatory factor in chondrocytes, but the mechanism underlying ADSCs and $IL-1{\beta}$ is unclear. In this study, we investigate whether P2X7 receptor (P2X7R) signalling, regulated by microRNA 373 (miR-373), was involved in the ADSCs and $IL-1{\beta}$ mediated inflammation in osteoarthritis (OA). Chondrocytes were collected from 20 OA patients and 20 control participants, and ADSCs were collected from patients who had undergone abdominal surgery. The typical surface molecules of ASDCs were detected by flow cytometry. The level of nitric oxide (NO) was determined by Griess reagent. Concentrations of prostaglandin E2 (PGE2), interleukin 6 (IL-6), matrix metallopeptidase 3 (MMP-3) were detected by enzyme-linked immunosorbent assay (ELISA). The expressions of IL-6, MMP-3, miR-373 and P2X7R were determined by real-time polymerase chain reaction (PCR), and Western blot was used to detect the protein expression of P2X7R. The typical potential characters of ADSCs were verified. In chondrocytes or OA tissues, the miR-373 expression level was decreased, but the P2X7R expression was increased. $IL-1{\beta}$ stimulation increased the level of inflammatory factors in OA chondrocytes, and ADSCs co-cultured with $IL-1{\beta}$-stimulated chondrocytes decreased the inflammation. OA chondrocytes transfected with the miR-373 inhibitor increased the inflammation level. The miR-373 mimic suppressed the inflammation by targeting P2X7R and regulated its expression, while its effect was reversed by overexpression of P2X7R. $IL-1{\beta}$ induced inflammation in OA chondrocytes, while ADSCs seemed to inhibit the expression of P2X7R that was regulated by miR-373 and involved in the anti-inflammatory process in OA.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2007.11a
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• pp.938-942
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• 2007

The importance of process improvement under construction has arisen from recent issue, lower productivity in the construction site. The various 3D modeling program is utilized in the procedure of construction as an alternative solution. However, it's still shortage of the consideration about a specific technical application. The purpose of the study in this paper is helpful to improve the productivity of construction site using 3D realization of constructing place as one of extensive modeling technologies, which leads to not only efficient management of construction site allowing people to check the real time situation in the place but also the revitalization of information flow about building process control and prgress, Therefore, I research into modeling algorithm and extensive construction site realization technology. 3D realization of building place would reduce the safety concerns by providing the real time information about construction site, and it could help to access easily to similar project through collecting and appling the database of sites. Furthermore it can be an opportunity to develop the procedure of production in construction industry and to upgrade the image of this field.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.3
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• pp.679-685
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• 2004

Although the electrophoretic deposition method has the advantage of simple processing procedure, less fabrication facilities, and easier control for deposition thickness and wire length, providing economical and technical merits, it also has the disadvantages of cracking and porosity phenomena, requiring an improved processing method for higher particle density and constant particle orientation. we have developed an optimization method to increase the particle density and to unify its orientation, and have performed a study to overcome the cracking and porosity problems in the fabricated superconductor. In order to improve the surface uniformity and the conduction properties of the fabricated YBCO thick films, a system that applies alternate voltage vertically has been developed for the first time and applied to the electrophoretic deposition process. The applied alternate electric field caused a force to be exerted on each YBCO particle and resulted in a rotation of the particle in the direction of applied electric field, accomplishing a uniform particle orientation. We name this process as the shaky-aligned electrophoretic deposition method. For commercial utilization and efficiency, in this dissertation, alternating voltage of 60 Hz and 25 ∼ 120 V/cm was proposed to apply it as a subsidiary source for shaky-flow deposition so that the fabricated thin film showed uniform surface morphology with less voids and cracks and Tc,zero of 90 K and the critical current density of 3419 A/$cm^2$.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.305-315
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• 2016

Due to the change from small volume production to small quantity batch production systems, individual companies have been attempting to produce a wide range of operating strategies, maximize their productivity, and minimize their WIP level by operating with the proper cycle time to defend their market share. In particular, using a complex workflow and process sequence in the manufacturing line has some drawbacks when it comes to designing the production strategy by applying analytical models, such as mathematical models and queueing theory. For this purpose, this paper uses three heuristic algorithms to solve the job release problem at the bottleneck workstation, product mix problem in multi-purpose machine(s), and batch size and sequence in batch machine(s). To verify the effectiveness of the proposed methods, a simulation analysis was performed. The experimental results demonstrated that the combined application of the proposed methods showed positive effects on the reduction of the cycle time and WIP level, and improvement of the throughput.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.4
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• pp.44-50
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• 2016

A study to apply phase change material(PCM) to rapid hardening cement paste forming semi-rigid pavement was carried out. The characteristics fresh and hardened paste were evaluated through the experiment for a total of 6 mixtures according to the cement type and the substitution of phase change material for acrylate. The fluidity by substituting phase change material for acrylate satisfied the target flow time of 10 to 13 seconds. In case of setting time, it was possible to secure the performance of rapid hardening cement by substituting phase change material, and if the substitution ratio over 60%, the initial set occurred 1 to 2 minutes faster than other mixtures. In case of compressive strength and bond strength, it showed similar strength characteristics with the plain mixture, and it satisfied both the target compressive and bonding strength of 36MPa and 2MPa. The mixture substituting phase change material showed higher resistance to chloride ion penetration than the mixture only using acrylate and the OPC level was insufficient. From the results of physical and mechanical performances of semi-rigid pavement cement paste, the phase change material substitution rate of 20% was effective in the range of this study.

• KIPS Transactions on Computer and Communication Systems
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• v.3 no.8
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• pp.261-270
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• 2014

As Internet traffic increases recently, much effort has been put on improving the performance of a web server. In addition to hardware side solutions such as replacement by high-end hardware or expansion of the number of servers, there are software side solutions to improve performance. Recent studies on these software side solutions have been actively performed. In this paper, we identify performance degradation problems occurring in a conventional TCP networking reception process and propose a way to solve them. We improve performance by combining three kinds of existing methods for Linux Networking Performance Improvement and two kinds of newly proposed methods in this paper. The three existing methods include 1) an allocation method of a packet flow to a core in a multi-core environment, 2) ITR(Interrupt Throttle Rate) method to control excessive interrupt requests, and 3) sk_buff data structure recycling. The two newly proposed methods are fd data structure recycling and epoll_event data structure recycling. Through experiments in a web server environment, we verify the effect of our two proposed methods and its combination with the three existing methods for performance improvement, respectively. We use three kinds of web servers: a simple web server, Lighttpd generally used in Linux, and Apache. In a simple web server environment, fd data structure recycling and epoll_event data structure recycling bring out performance improvement by about 7 % and 6%, respectively. If they are combined with the three existing methods, performance is improved by up to 40% in total. In a Lighttpd and an Apache web server environment, the combination of five methods brings out performance improvement by up to 36% and 20% in total, respectively.

• The KIPS Transactions:PartC
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• v.8C no.6
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• pp.793-805
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• 2001

In the recent priority system, high-priority packet will be served first and low-priority packet will be served when there isn\`t any high-priority packet in the system. By the way, even high-priority packet can be blocked by HOL (Head of Line) contention in the input queueing System. Therefore, the whole switching performance can be improved by serving low-priority packet even though high-priority packet is blocked. In this paper, we study the performance of preemptive priority in an input queueing switch for high speed switch system. The analysis of this switching system is taken into account of the influence of priority scheduling and the window scheme for head-of-line contention. We derive queue length distribution, delay and maximum throughput for the switching system based on these control schemes. Because of the service dependencies between inputs, an exact analysis of this switching system is intractable. Consequently, we provide an approximate analysis based on some independence assumption and the flow conservation rule. We use an equivalent queueing system to estimate the service capability seen by each input. In case of the preemptive priority policy without considering a window scheme, we extend the approximation technique used by Chen and Guerin [1] to obtain more accurate results. Moreover, we also propose newly a window scheme that is appropriate for the preemptive priority switching system in view of implementation and operation. It can improve the total system throughput and delay performance of low priority packets. We also analyze this window scheme using an equivalent queueing system and compare the performance results with that without the window scheme. Numerical results are compared with simulations.