• 한국양식학회지
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• 제9권4호
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• pp.377-384
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• 1996

실용적인 사료의 효과를 검토하기 위해 단백질함량이 $41\~45\%$인 4종류의 실험사료를 dry pellet 형태로 제조하였다. 단백질원으로 어분을 $58\%$ 첨가한 사료를 대조구(사료 1)로 하고, 대조구의 어분을 육분, 콘글루텐 밀, 대두박 및 혈분으로 각각 다른 비율로 대체하여 어분 함량을 $40\%$ 전후로 감소시킨 실험사료들(사료 $2\~4$)을 설계하여 시판되고 있는 해산어용 배합사료 A, B, C (단백질 함량 : $51\~53\%$) 및 양어가들이 선호하는 RMP 사료(넙치용 분말사료와 냉동 메가리를 1 : 1의 비율로 혼합한 moist pellet, 단백질 함량 : $58\%$)와 그 효과를 사육 실험을 통해 비교하였다. 평균체중 125 g의 조피볼락을 선별하여 실험사료마다 2반복으로 15주간 사육실험한 결과, 최종 평균체중, 증체율, 사료효율, 일일사료섭취율, 일일단백질섭취율, 단백질효율 및 간 중량지수가 RMP 구를 제외한 실험사료와 상품사료를 급여한 실험구들은 대조구와 통계적인 차이는 없었다(P>0.05). RMP 섭취구의 증체율이 대조구와는 유의한 차이가 없었지만, 실험사료(사료 $2\~4$)와 상품사료(사료 $A\~C$)보다 유의적으로 높은 값을 보였다(P<0.05). 또한, 단백질효율은 RMP 구가 가장 높았고, 상품사료 B가 가장 낮은 값을 보였다. 대조사료에서 1 kg의 어체를 증체시키는데 소요되는 사료비용을 100으로 환산하였을 때, 사료 3 급여구는 92, 사료 4 급여구는 79로 나타나, 대조구보다 $10\~20\%$ 정도의 사료비가 절감되었고, RMP 사료 급여구는 대조구보다 $27\%$의 사료비가 더 소요되는 것으로 나타났다. 실험 종료시 간과 근육의 수분, 단백질, 지질 및 회분 함량은 모든 실험구간에 유의차는 없었다(P>0.05).

• 한국수산과학회지
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• 제45권1호
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• pp.50-55
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• 2012

We compared the nutritional requirements of whole larvae of the black seabream Acanthopagrus schlegeli, sweet fish Plecoglossus altivelis, olive flounder Paralichthys olivaeus and rock fish Sebastes schlegeli. The larvae were 20, 30, 14 and 5 DAH (or spawning) of black seabream, sweet fish, olive flounder and rock fish, respectively. Specific ${\alpha}$-amylase activity (mU/mg protein) was highest (8,324.9 mU/mg protein) in rock fish larvae (P<0.05). Specific trypsin-like protease activity was highest (11,330.1 mU/mg protein) in black seabream larvae (P<0.05), which also exhibited the highest activity, 685.5 mU/mg dry weight (P<0.05). The specific activities per mg protein and mg dry weight of black seabream were the highest (187.4 mU/mg protein and 11.3 mU/mg dry weight, respectively) (P<0.05). A/P, P/L and A/L ratios of rock fish were 1.47, 90.3 and 133.1, respectively (P<0.05). We present here basic larval digestive enzymatic nutritional requirement data.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1367-1375
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• 2008

This study was conducted to investigate the effects of phytase in diets containing cottonseed and soybean meal (CS) on growth performance, feed utilization and digestibility of protein and phosphorus in juvenile olive flounder (initial body weight 2.5 g), Paralichthys olivaceus. Four experimental diets replacing 0%, 30%, 30% and 40% fish meal protein with CS in equal proportion were formulated to be isonitrogenous and isocaloric (designated as CS0, CS30, CS30+P, CS40+P, respectively). Phytase of 1,000 FTU/kg was supplemented in diets CS30+P and CS40+P. Three groups of fish (25 fish per group) were fed one of the experimental diets for 10 weeks. No significant differences were observed in growth performance of fish groups except for the CS40+P diet. Apparent digestibility coefficients of protein and phosphorus in fish fed phytase-containing diets were significantly higher than those of fish fed the CS0 diet. Serum cholesterol was significantly reduced in fish fed the CS-containing diets. Antioxidant activities in the diets and liver of fish were significantly increased with the increment of dietary CS. Gossypol was only detected and found in liver of the fish fed the CS-containing diets. The findings suggest that supplementation of microbial phytase could improve the apparent digestibility of protein and phosphorus in juvenile olive flounder fed the CS-containing diets.

• 한국수산과학회지
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• 제43권4호
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• pp.314-319
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• 2010

This study determined the proper inclusion levels of squid liver powder (SLP) - soy protein concentrate (SPC) premix as a fish meal (FM) substitute in the diet for juvenile olive flounder (Paralichthys olivaceus). All the experimental diets were formulated to be isonitrogenous (50% crude protein) and isocaloric (17.1 kJ energy $g^{-1}$ diet). Five diets were formulated with SLP-(SPC) replacing 0, 25, 50, 75, and 100% of the FM (SLP $(SPC)_0$, SLP $(SPC)_25$, SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP $(SPC)_100$, respectively). Triplicate groups of 12 fish averaging $5.56{\pm}0.10\;g$ ($mean{\pm}SD$) were fed one of the five experimental diets for 7 weeks. The weight gain (WG), specific growth rate (SGR), feed efficiency (FE), and protein efficiency ratio (PER) of fish fed SLP $(SPC)_0$ were significantly (P<0.05) higher than those of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$ and SLP $(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude protein content of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP$(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude lipid of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$ and SLP $(SPC)_75$, while those of fish fed SLP $(SPC)_0$, SLP $(SPC)_25$ and SLP $(SPC)_100$ were similar. Based on the fish growth performance results obtained from this study, the SLP (SPC) premix can substitute up to 25% of the FM in the diets of juvenile olive flounder.

• 한국어병학회지
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• 제18권3호
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• pp.215-225
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• 2005

본 연구는 강독성 균주인 E. tarda PoKF-000623가 생산하는 세포외생성물 (ECPs)을 넙치에 투여되었을 때 나타나는 생리학적 및 면역학적 영향을 조사하고자 하였다. E. tarda PoKF-000623 균주가 생성하는 ECPs의 넙치 (평균체중, 5.6g)에 대한 96hr-$LD_{50}$ 은 약 0.714${\mu}g$ g TEX>$fish^{-1}$ 이었으며, ECPs의 주사에 의한 폐사는 고농도 (40${\mu}g$ TEX>$fish^{-1}$) 뿐만 아니라 저농도 (4${\mu}g$ TEX>$fish^{-1}$)에서도 지속적으로 발생하였다. 평균 체중 59.1 g 크기의 넙치에 각각 0, 4 and 40${\mu}g$ ECPs TEX>$fish^{-1}$ 의 농도로 근육 주사한 후, 혈청 중 glucose 농도와 total protein 농도 및 신장 식세포의 활성을 조사하였다. 그 결과 고농도의 ECPs 주사 시험구의 넙치에서 혈중 glucose 농도와 total protein 농도가 대조구에 비하여 유의적으로 높게 나타났다. 그러나 세포성 면역 기구인 신장 식세포의 CL 반응과 세균 살해능은 대조구에 비하여 유의적으로 저하되었다. 따라서 넙치에 고농도의 E. tarda ECPs를 투여하면 생체 지표의 일부에 영향을 미치는 것으로 확인되었고 면역 활성, 특히 신장 식세포 활성의 억제가 edwardsielosis의 발병에 영향을 미치는 것으로 사료된다.

• 한국식품영양과학회지
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• 제1권1호
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• pp.51-62
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• 1972

In Korea, fermented fish has been playing an important role as a preserved and flavor rich food. It is said that the digestion of fish protein is due to both action of intrinsic (autolytic enzymes) and bacterial enzymes in fish. The mass production of fermented fish has been impeded since traditional method of fermentation requires a long duration for a complete digestion. A high concentration of salt and unsanitary condition are also considered disadvantages of the old method. To improve the quality of the product and to develop mechanized process of fermentation, fermentors which have such control device as temperature, pH and agitation control system have been urgently needed. In this study, a model design of a fermentor is studied. The calculation was based on the optimum conditions for enzymatic hydrolysis of fish protein which involve temperature, pH, viscosity and other factors.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.175-176
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• 2003

Dietary protein is the most expensive component in flounder feed because of the high protein requirement of this species. The non-protein energy sources may also influence the protein utilization of fish. The inclusion of adequate levels of non-protein energy sources in diets can minimize use of protein as an energy source. Protein sparing effect by fat and carbohydrate has been studied in other fish. (omitted)

• 한국양식학회지
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• 제10권2호
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• pp.143-151
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• 1997

본 연구는 조피볼락 사료에 있어서 어분대체품을 결정하고, 상품어분대체품과 유인물질의 첨가 효과를 통하여 조피볼락용 어분대체품개발을 위한 기초자료도 사용하고자 수행되었다. 실험사료는 조단백질 52%로, 가용성 에너지 16.8 KJ/g으로 설계되었고, 주단백질원으로 북양어분, 어분대체품 및 상품어분대체품을 사용하였다. 사료의 성분조성은 다음과 같이 요약된다 : 사료1, 100%FM (control) ; 사료2, 60%FM : 40% CFMA ; 사료3, 60%FM : 40% CFMA+ATT ; 사료 4, 80%FM : 20% FMA ; 사료5, 80% FM: 20%FMA+ATT : 사료 6, ; 사료6, 60%FM : 40%FMA+ATT; 사료7, 40%FM : 60% FMA+ATT. 어분대체품은 혈분(BM), 오징어간분(SLP), 육골분(MBM), 수지박(LM), 가금부산물(PBP), 우모분(FM), 필수아미노산(Met, Lys, Ile) 등을 적절히 배합하여 제작 사용하였다. 성장률, 사료효율, 일간성장률 및 단백질효율에 있어서 사료 1은 사료 4, 사료 5 및 사료 6에 비해 유의적인 차이가 없었지만(P>0.05), 나머지 실험구들은 대조구에 비해 유의적으로 낮게 나타났다(P<0.05). 특히, 상품어분대체품(CFMA)인 사료 2와 사료 3은 실험사료로 제작된 동일한 수준의 사료 6과 비교하여 성장률, 사료효율, 단백질효율에서 유의적으로 낮은 경향을 보였다. 0.5% 유인물질을 첨가한 사료 3과 5는 사료 2와 4에 비해 유의적인 차이는 보이지 않았으며(P>0.05), 간중량지수, hemoglobin 및 비만도에 있어서는 유의적인 차이가 나타났다(P<0.05). 따라서, 조피볼락에 있어서 어분대체품(FMA)으로 어분단백질을 40%정도 대체 가능함을 알 수 있었다.

• 한국수산과학회지
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• 제42권5호
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• pp.476-480
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• 2009

This study was conducted to evaluate the effect of extruded pellets (EP) containing different levels of protein (51%-55%) and lipid (9%-15%) for growth of flounder (Paralichthys olivaceus) comparing with raw fish-based moist pellet (MP). Two replicate groups of 40 fish per each tank (initial mean weight 106 g) were fed one of three experimental EP (EP1, EP2 and EP3) containing different protein and lipid levels, a commercial EP (EP4) and MP for 16 weeks. Survival was not significantly different among all groups. Final mean weight of fish fed MP was significantly lower than that of fish fed EP1, EP2 and EP4 (P<0.05), but not significantly different from fish fed EP3. Feed efficiency of MP-fed fish was significantly lower than fish fed all EP formulations (P<0.05), but no significant difference was observed among the EP groups. Daily feed intake of MP-fed fish was significantly higher than fish fed all EP formulations (P<0.05). Condition factor was not significantly different among all groups. Whole body moisture and crude lipid contents were significantly affected by diet (P<0.05). Growth and feed efficiency of flounder was not affected by EP protein and lipid levels. Dietary formulation used in EP1, EP2 and EP3 can be applied to the practical feeding of flounder.

• 한국양식학회지
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• 제18권1호
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• pp.60-65
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• 2005

Effects of the commercially available or formulated extruded pellets (EP) and raw fish-based moist pellet (MP) on growth and body composition of flounder Paralichthys olivaceus L. were evaluated on commercial scale for 10 months. Survivals of flounder fed the MP, EP 1 and EP3 were not significantly different from those of fish fed the EP2 or EP5, but significantly (P<0.05) higher than that of fish fed the EP4. Weight gain of fish fed the MP was not significantly different from that of fish fed the EP3 and EP5, but significantly (P<0.05) higher than that of fish fed the EP1, EP2 or EP4. Improvement in weight gain of flounder fed the high lipid diets (EP1, EP2, EP3) compared to low lipid diet (EP4) in the $55\%$ protein level, and weight gain of fish fed the high lipid diet (MP) compared to low lipid diet (EP5) in the $61\%$ protein level indicated protein-sparing effect of lipid. FER for flounder fed the EP3. which was not significantly different from that for fish fed the EP1 or EP5 was significantly (P<0.05) higher thar. that for fish fed the EP2, EP4 and MP. FER significantly (P<0.05) changed over time. CF of flounder fed the MP was significantly (P<0.05) higher than that of fish fed EP1 or EP4. Moisture content of dorsal muscle in flounder fed the EP2 was significantly (P<0.05) higher than that in fish fed the EP1 or EP4. Crude protein content of dorsal. muscle in flounder fed the EP1, EP4 and EP5 was significantly (P<0.05) higher than that in fish fed the EP2. How- ever, crude lipid content of dorsal muscle, and moisture, crude protein and crude lipid content of liver in flounder was not significantly different among the diets. In considering performance of flounder, it can be concluded that the use of EP3 and EP5 is more recommendable than the MP, which commonly used in flounder farm.