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Effect of extracellular products (ECPs) of Edwardsiella tarda on olive flounder, Paralichthys olivaceus  

Lee, Deok-Chan (Pathology Team, National Fisheries Research and Development Institute)
Kim, Yi-Cheong (Pathology Team, National Fisheries Research and Development Institute)
Kim, Jin-Woo (Pathology Team, National Fisheries Research and Development Institute)
Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.18, no.3, 2005 , pp. 215-225 More about this Journal
Abstract
The effect of extracellular products (ECPs) prepared from highly virulent Edwardsiella tarda PoKF-000623 on the physiological and the immunological function in the olive flounder, Paralichthys olivaceus was evaluated. The virulence of ECPs on the fish, 5.6 g of average body weight was detected $0.714{\mu}{g}$ g $fish^{-1}$ as $96hr-LD_{50}$ by intraperitonial injection. After intramuscularly injected with 0, 4 and $40{\mu}{g}$ ECPs TEX>$fish^{-1}$ to fish average sized 59.1 g of body weight, the fish were measured the glucose and total protein concentration in sera, and immune activity of kidney macrophage. The fish injected with $40{\mu}{g}$ protein $fish^{-1}$ showed a significant increase in the glucose and total protein concentration in sera. But, the fish were changed in cellular immune response as lowed chemiluminescence response and bactericidal activity of head kidney macrophage. These results were suggested that ECPs of E. tarda could be effect on the physiological and the immunological factors, especially in the function of kidney macrophage concerning to edwardsiellosis infection.
Keywords
Edwardsiella tarda; Extracellular products; ECPs; Paralichthys olivaceus; Glucose; Total protein; Chemiluminescence response; Bactericidal activity;
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