• Genomics & Informatics
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• 제20권1호
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• pp.2.1-2.11
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• 2022

The method of single-cell RNA sequencing has been rapidly developed, and numerous experiments have been conducted over the past decade. Their results allow us to recognize various subpopulations and rare cell states in tissues, tumors, and immune systems that are previously unidentified, and guide us to understand fundamental biological processes that determine cell identity based on single-cell gene expression profiles. However, it is still challenging to understand the principle of comprehensive gene regulation that determines the cell fate only with transcriptome, a consequential output of the gene expression program. To elucidate the mechanisms related to the origin and maintenance of comprehensive single-cell transcriptome, we require a corresponding single-cell epigenome, which is a differentiated information of each cell with an identical genome. This review deals with the current development of single-cell epigenomic library construction methods, including multi-omics tools with crucial factors and additional requirements in the future focusing on DNA methylation, chromatin accessibility, and histone post-translational modifications. The study of cellular differentiation and the disease occurrence at a single-cell level has taken the first step with single-cell transcriptome and is now taking the next step with single-cell epigenome.

• 생물정신의학
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• 제27권2호
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• pp.58-63
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• 2020

Depressive disorder is a very common disease, clinical manifestations vary, and the mechanism is not clear. Therefore, a pharmacotherapy is very important to achieve sufficient therapeutic effect, but the choice of drug is not easy due to the occurrence of side effects of treatment and confusion with clinical features. It is easy to overlook the side effects of weight gain with antidepressants compared with antipsychotics, but they are frequently observed in clinical settings. The first-generation antidepressants have higher weight gains than selective serotonin reuptake inhibitors. Serotonin norepinephrine reuptake inhibitors are observed to have less weight gain, and dopamine norepinephrine reuptake inhibitors have weight loss effect due to decreased appetite. Mirtazapine, an atypical antidepressant, has a strong histamine H1 blockade, and gains weight gain from short-term use. The effects of desvenlafaxine, vortioxetine, and agomelatin on weight, which have recently been increasing in use, have not been largely identified. For better compliance, studies on weight gain due to the use of antidepressants are needed.

• 한국균학회지
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• 제51권4호
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• pp.447-460
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• 2023

식물병원 세균 Erwinia amylovora 의해 발생하는 과수화상병은 사과, 배 등 장미과에 속하는 식물에 심한 피해를 주고 있다. 병의 빠른 확산을 막기 위해 국내에서는 병이 발생한 사과나무와 배나무에 대해 매몰하는 병관리를 실시해 왔다. 방제방법의 안전성을 입증하기 위해 매몰한 이병식물과 이병식물 주변의 토양을 조사하였다. 매몰토양 미생물군 조사과정에서 Bisifusarium allantoides, Botryotrichum Domesticum, Microascus verrucosus, Paraphoma pye, Phaeosphaeria culmorum, Ramophialophora globispora, Sordaria tomentoalba, Striaticonidium brachysporum 등이 국내 미기록종으로 확인되었다. 이에 이들 8종 진균에 대한 형태학적, 분자유전학적 특성을 보고한다.

• The Plant Pathology Journal
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• 제22권1호
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• pp.51-62
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• 2006

Three strains of Cucumber mosaic virus (CMV) have been found to cause a lethal disease, referred to as fern leaf syndromes and mild mosaic symptoms in tomato (Lycopersicon esculentum Mill.) crops grown in Kuwait. CMV strains were detected and identified based on host range, symptomatology, serology, electron microscopy, and ribonucleic acid (RNA) electrophoresis in polyacrylamide gels. A high degree of viral genomic heterogeneity was detected among CMV strains isolated in Kuwait, with no apparent correlation to symptomatology in tomato host plants. Two different virus satellites of 'CMV associated RNA 5', designated CARNA 5, were detected in two virus strains that caused both lethal disease and mild symptoms, designated CMV-D1 and CMV-S1 respectively. CARNA5 was not detected in the third CMV strain that caused fern leaf syndromes designated CMV-F. All the three isolated strains were serologically indistinguishable from each other and may belong to one serotype according to Ouchterlony gel diffusion tests. These strains transmitted via aphids (Myzus persicae Sulz) in a non-persistent manner. Physical properties of the virus strains were very similar where thermal inactivation test showed that virus withstood heating for 10 min at $70^{/circ}$, dilution end point was $10^{-4}$, and the longevity in vitro at room temperature was less than 5 days for all virus strains. CMV-D1 and CMV-F were the most devastating diseases spreading in both greenhouse and field-grown tomato where aborted flower buds failed on fruit setting due to the viral infection. This is the first report to isolate three different strains of CMV in Kuwait.

• The Plant Pathology Journal
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• 제30권4호
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• pp.416-424
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• 2014

Sweet potato is grown extensively from tropical to temperate regions and is an important food crop worldwide. In this study, we established detection methods for 17 major sweet potato viruses using single and multiplex RT-PCR assays. To investigate the current incidence of viral diseases, we collected 154 samples of various sweet potato cultivars showing virus-like symptoms from 40 fields in 10 Korean regions, and analyzed them by RT-PCR using specific primers for each of the 17 viruses. Of the 17 possible viruses, we detected eight in our samples. Sweet potato feathery mottle virus (SPFMV) and sweet potato virus C (SPVC) were most commonly detected, infecting approximately 87% and 85% of samples, respectively. Furthermore, Sweet potato symptomless virus 1 (SPSMV-1), Sweet potato virus G (SPVG), Sweet potato leaf curl virus (SPLCV), Sweet potato virus 2 ( SPV2), Sweet potato chlorotic fleck virus (SPCFV), and Sweet potato latent virus (SPLV) were detected in 67%, 58%, 47%, 41%, 31%, and 20% of samples, respectively. This study presents the first documented occurrence of four viruses (SPVC, SPV2, SPCFV, and SPSMV-1) in Korea. Based on the results of our survey, we developed multiplex RT-PCR assays for simple and simultaneous detection of the eight sweet potato viruses we recorded.

• 한국균학회지
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• 제29권1호
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• pp.72-74
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• 2001

2000년 9월 상순경 경상남도 의령군 가례면 밭 미나리 재배포장에서 미나리 흰비단병(Sclerotium rolfsii)이 대발생하였다. 이 병의 병징은 미나리 줄기 절단부가 갈색으로 변하고 물러져 썩으며 그 위에 흰색의 곰팡이가 솜털처럼 밀생하고, 병발부위에 작은 갈색의 둥근 균핵을 형성하며 지제부 부근의 토양 표면에도 형성되었다. 균사의 폭은 $4.1{\sim}10.3{\mu}m$이고, 균사 생육적온은 $30^{\circ}C$에서 90.0 mm로 가장 잘 자랐으며 $5^{\circ}C$와 $45^{\circ}C$에서는 자라지 않았다. PDA 배지상에는 $20^{\circ}C$에서 균핵을 많이 형성하였다. 균총의 색깔은 흰색이며 clamp connection이 있는 것이 특징이다. 균핵의 모양은 구형이며 크기는 $1.0{\sim}6.3{\times}10{\sim}5.2mm$ (평균 $2.4{\sim}2.2mm$)이었다. 이 병원균을 Sclerotium rolfsii로 동정하였고, 미나리 흰비단병으로 명명할 것을 제안한다.

• Parasites, Hosts and Diseases
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• 제44권4호
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• pp.285-294
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• 2006

Changing patterns of the reemerging Plasmodium vivax malaria in the Republic of Korea (South Korea) during the period 1993 to 2005 are briefly analyzed with emphasis on the control measures used and the effects of meteorological and entomological factors. Data were obtained from the Communicable Diseases Monthly Reports published by the Korea Center for Disease Control and Prevention, and webpages of World Health Organization and United Nations. Meteorological data of Kangwon-do (Province) were obtained from local weather stations. After its first reemergence in 1993, the prevalence of malaria increased exponentially, peaking in 2000, and then decreased. In total, 21,419 cases were reported between 1993 and 2005 in South Korea. In North Korea, a total of 916,225 cases were reported between 1999 and 2004. The occurrence of malaria in high risk areas of South Korea was significantly (P < 0.05) correlated with the mosquito population but not with temperature and rainfall, Control programs, including early case detection and treatment, mass chemoprophylaxis of soldiers, and international financial aids to North Korea for malaria control have been instituted. The situation of the reemerging vivax malaria in the Republic of Korea is remarkably improving during the recent years, at least in part, due to the control activities undertaken in South and North Korea.

• 식물병연구
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• 제18권3호
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• pp.255-258
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• 2012

2011년 9월, 충남 보령시와 부여군의 시설재배단지에서 재배중인 토마토의 잎에 Pseudocercospora fuligena에 의한 검은잎곰팡이병이 발생하였다. 이 병의 병징은 초기에는 잎에 연한 노란색의 불규칙한 점무늬가 발생하며, 점차 연한 갈색으로 변하고 잎 뒷면의 병반부위에 병원균의 분생포자를 다량으로 형성하면서 검은색의 그을음 병징을 나타내었다. 병든 토마토 식물체로부터 원인균을 분리하였고 현미경 검경을 통하여 형태적 특징을 분석한 결과 P. fuligena로 동정되었다. 온실조건에서 인공접종을 통하여 이 균의 병원성을 증명하였다. P. fuligena에 의한 토마토 검은잎곰팡이병의 발생은 우리나라에서 최초의 보고이다.

• Journal of Korean Neurosurgical Society
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• 제42권3호
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• pp.184-190
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• 2007

Objective : The authors retrospectively analyzed clinical and radiographic features of patients who developed symptomatic adjacent segment degeneration (ASD) that required re-operation. Methods : From 1995 to 2004, among 412 patients who underwent posterior lumbar fusion surgery, the authors experienced twenty-six patients who presented symptomatic ASD. Records of these patients were reviewed to collect clinical data at the first and second operations. Results : The patients were 9 males and 17 females whose mean age was $63.5{\pm}8.7$ years. Among 319 one segment and 102 multi-segment fusions, 16 and 10 patients presented ASD, respectively. Seventeen ASDs were noticed at the cephalad to fusion (65%), eight at the caudad (31%), and one at the cephalad and caudad, simultaneously (4%). All patients underwent decompression surgery. Nine patients underwent additional fusion surgeries to adjacent degenerated segments. In 17 patients who underwent only decompression surgery without fusion, the success rate was 82.4%. In fusion cases. the success rate was observed as 55.5%. There were no statistically significant factors to be related to development of ASD. However, in cases of multi-level fusion surgery, there was a tendency toward increasing ASD. Conclusion : Multi-segment fusion surgery could be associated with a development of ASD. In surgical treatment of symptomatic ASD, selective decompression without fusion may need to be considered as a primary procedure, which could reduce the potential risk of later occurrence of the other adjacent segment disease.

• 한국균학회지
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• 제33권2호
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• pp.89-91
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• 2005

2003년에서 2004년까지 4월 경남농업기술원 함양약초 시험포장 노지재배 중인 물레나물에서 이상증상이 심하게 발생하였다. 병징은 지제부위의 줄기 부분이 수침상으로 물러지고 썩으면서 병반부위에 회색의 곰팡이가 많이 생기고, 심한 경우 감염된 부위는 시들어 말라 죽는다. 분생 포자는 무색, 단포자이며 난형 또는 타원형으로 크기는 $4{\sim}16{\times}3{\sim}8\;{\mu}m$였고, 분생포자경 위에 분생포자가 아주 많이 형성되었다. 분생포자경은 갈색으로 격막이 있고, 폭은 $12{\sim}26\;{\mu}m$였다. 균사생육과 균핵형성 적온은 $20^{\circ}C$였다. 병원성 검정결과 코호가설을 충족시켰다. 병반에서 분리한 병원균의 균학적 특징을 조사한 결과 Botrytis cinerea Persoon: Fries로 동정되었다. Botrytis cinerea에 의한 물레나물 잿빛곰팡이병을 국내에서 처음 보고한다.