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http://dx.doi.org/10.5808/gi.21078

Experimental development of the epigenomic library construction method to elucidate the epigenetic diversity and causal relationship between epigenome and transcriptome at a single-cell level  

Park, Kyunghyuk (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Jeon, Min Chul (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Kim, Bokyung (Department of Obstetrics and Gynecology, Seoul National University Hospital)
Cha, Bukyoung (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Kim, Jong-Il (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Publication Information
Genomics & Informatics / v.20, no.1, 2022 , pp. 2.1-2.11 More about this Journal
Abstract
The method of single-cell RNA sequencing has been rapidly developed, and numerous experiments have been conducted over the past decade. Their results allow us to recognize various subpopulations and rare cell states in tissues, tumors, and immune systems that are previously unidentified, and guide us to understand fundamental biological processes that determine cell identity based on single-cell gene expression profiles. However, it is still challenging to understand the principle of comprehensive gene regulation that determines the cell fate only with transcriptome, a consequential output of the gene expression program. To elucidate the mechanisms related to the origin and maintenance of comprehensive single-cell transcriptome, we require a corresponding single-cell epigenome, which is a differentiated information of each cell with an identical genome. This review deals with the current development of single-cell epigenomic library construction methods, including multi-omics tools with crucial factors and additional requirements in the future focusing on DNA methylation, chromatin accessibility, and histone post-translational modifications. The study of cellular differentiation and the disease occurrence at a single-cell level has taken the first step with single-cell transcriptome and is now taking the next step with single-cell epigenome.
Keywords
cellular heterogeneity; chromatin accessibility; DNA methylation; histone post-translational modifications (PTMs); single-cell epigenome; single-cell multiome;
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