Browse > Article
http://dx.doi.org/10.5423/PPJ.2006.22.1.051

Occurrence of Three Strains of Cucumber mosaic virus Affecting Tomato in Kuwait  

Montasser Magdy Shaban (Department of Biological Sciences, Faculty of Science, University of Kuwait)
Dashti Narjes Haji (Department of Biological Sciences, Faculty of Science, University of Kuwait)
Ali Neda Yousef (Department of Biological Sciences, Faculty of Science, University of Kuwait)
Bhardwaj Radhika Guleri (Department of Biological Sciences, Faculty of Science, University of Kuwait)
Al-Hamar Bader (Department of Biological Sciences, Faculty of Science, University of Kuwait)
Publication Information
The Plant Pathology Journal / v.22, no.1, 2006 , pp. 51-62 More about this Journal
Abstract
Three strains of Cucumber mosaic virus (CMV) have been found to cause a lethal disease, referred to as fern leaf syndromes and mild mosaic symptoms in tomato (Lycopersicon esculentum Mill.) crops grown in Kuwait. CMV strains were detected and identified based on host range, symptomatology, serology, electron microscopy, and ribonucleic acid (RNA) electrophoresis in polyacrylamide gels. A high degree of viral genomic heterogeneity was detected among CMV strains isolated in Kuwait, with no apparent correlation to symptomatology in tomato host plants. Two different virus satellites of 'CMV associated RNA 5', designated CARNA 5, were detected in two virus strains that caused both lethal disease and mild symptoms, designated CMV-D1 and CMV-S1 respectively. CARNA5 was not detected in the third CMV strain that caused fern leaf syndromes designated CMV-F. All the three isolated strains were serologically indistinguishable from each other and may belong to one serotype according to Ouchterlony gel diffusion tests. These strains transmitted via aphids (Myzus persicae Sulz) in a non-persistent manner. Physical properties of the virus strains were very similar where thermal inactivation test showed that virus withstood heating for 10 min at $70^{/circ}$, dilution end point was $10^{-4}$, and the longevity in vitro at room temperature was less than 5 days for all virus strains. CMV-D1 and CMV-F were the most devastating diseases spreading in both greenhouse and field-grown tomato where aborted flower buds failed on fruit setting due to the viral infection. This is the first report to isolate three different strains of CMV in Kuwait.
Keywords
CMV; Kuwait; Tomato;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kaper, J. M. 1993. Viral satellites, molecular parasites for plant protection. In: Pest management: Biologically based technologies. Proc. Beltsville Symp. XVIII, ed. by R. D. Lumsden and J. L. Vaughn, pp. 134-143. American Chemical Society, Washington, D. C
2 Milne, K. S., Grogan, R. G. and Kimble, K. A. 1969. Identification of viruses infecting cucurbits in California. Phytopathology 59:819-828
3 Mink, G. I., Iizuka, N. and Kiriyama, K. 1975. Some cucumber mosaic virus antisera contain antibodies specific for both peanut stunt virus and chrysanthemum mild mottle virus. Phytopathology 65:65-68   DOI
4 Orellana, R.G and Quacquarelli, A. 1968. Sunflower mosaic caused by a strain of cucumber mosaic virus. Phytopathology 58: 1439-1440
5 Roossinck, M. 1991. Temperature-sensitive replication of cucumber mosaic virus in muskmelon (Cucumis melo cv. Iroquois), maps to RNA 1 of a slow strain. J. Gen. Virol. 72:1747-1750   DOI   ScienceOn
6 Srivastava, K., Raj, S. and Singh, B. 1992. Properties of cucumber mosaic virus strain naturally infecting Chrysanthemum in India. Plant Dis. 76:474-477   DOI
7 Clark, M. and Adams, A. 1977. Characteristic of the microplate method of enzyme- linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483   DOI   ScienceOn
8 Gibbs, A. and Harrison, B. 1970. Cucumber mosaic virus. CMI/AAB Description of plant viruses. I
9 Jorda, C., Alfaro, A., Aranda, M., Moriones, E. and Garcia-Arenal, F. 1992. Epidemic of cucumber mosaic virus plus satellite RNA in tomatoes in Eastern Spain. Plant Dis. 76:363-366   DOI
10 Al-Awadhi, H. A., Hanif, A., Suleman, P. and Montasser, M. S. 2002. Molecular and microscopical detection of phytoplasma associated with yellowing disease of date palms Phoneix dactylifera L. in Kuwait. Kuwait J. Sci. Engineering 29:87-109
11 Kaper, J., Gallitelli, D. and Tousignant, M. 1990. Identification of 334-ribonucleotide viral sattellite as principal aetiological agent in tomato necrosis epidemic. Res. Virol.141:81-95   DOI   ScienceOn
12 Wahyuni, W., Dietzgen, R, Hanada, K. and Francki, R. 1992. Serological and biological variation between and within sub- group I and II strains of cucumber mosaic virus. Plant Pathol. 41 :282-297   DOI
13 Hadidi, A. and Yang, X. 1990. Detection of pome fruit viroids by enzymatic cDNA amplification. J. Virol. Methods 30:261-270   DOI   ScienceOn
14 Waterworth, H., Tousignant, M. and Kaper, J. 1978. A lethal disease of tomato experimentally induced by RNA-5 associated with cucumber mosaic virus isolated from California from E1 Salvador: Phytopathology 68:561-566   DOI
15 Crescenzi, A., Barbarossa, L., Cillo, F, Franco, A., Volvas, N. and Gallitelli, D. 1993. Role of cucumber mosaic virus and its satellite RNA in the etiology of tomato fruit necrosis in Italy. Arch. Virol. 131:321-333   DOI
16 Grogan, R., Hall, D. and Kimble, K. 1959. Cucurbit mosaic viruses in California. Phytopathology 49:366-376
17 Marrou, J., Duteil M., Lot, H. and Clerjeau, H. 1973. La necerose de la tomate: Une grave virose des tomates cultivees en plein champ. Pepin. Hortic. Maraich. 137:37-41
18 Montasser, M. S., Tousignant, M. E. and Kaper, J. M. 1998. Viral satellite RNAs for the prevention of cucumber mosaic virus (CMV) disease in field grown pepper and melon plants. Plant Dis. 82: 1298-1303   DOI
19 Seth, M. L. and Raychaudhuri, S. P. 1973. Further studies on a new mosaic diseases of brinjal (Solanum melongena L.). Proc. Indian Natl. Acad Sci. B 39: 122-128
20 Doolittle, S. P. 1916. A new infectious mosaic disease of cucumber. Phytopathology 6:145-147
21 Daniels, J. and Campbell, R. 1992. Characterization of cucumber mosaic virus isolates from California, Plant Dis. 76:1245-1250   DOI
22 Abou El-Ghar, A. E. 1969. Studies on some cucurbits virus diseases. M. Sc. thesis, Ain Shams Univ. A. R. E
23 Fulton, J. P. 1950. Studies on strains of cucumber virus I from spinach. Phytopathology 40:729-736
24 Kaper, J., Tousignant, M. and Steen, M. 1988. Cucumber mosaic virus-associated RNA 5. XI. Comparison of 14 CARNA 5 variants relates ability to induce tomato necrosis to a conserved nucleotide sequence. Virology 163:284-292   DOI   ScienceOn
25 Montasser, M. S. 1999. Experimental Protocols in Virology and Immunology, 1st ed., Academic Publication Council, Kuwait University, Kuwait
26 Gallitelli, D., Volvos, C., Martelli, G P., Montasser, M. S., Tousignant, M. E. and Kaper, J. M. 1991. Satellite mediated protection of tomato against cucumber mosaic virus. 11. Field test under natural epidemic conditions in Southern Italy. Plant Dis. 75:93-95   DOI
27 Kaper, J. and Waterworth, H. 1977. Cucumber mosaic virus associated RNA 5: causal agent for tomato necrosis. Science 196:429-431   DOI   ScienceOn
28 Reddy, K. R. C. and Nariani, T. K. 1963. Studies on mosaic diseases of vegetable marrow (Cucurbita pepo L.) Indian Phytopathol. 16:260-267
29 Simons, J. N. 1957. Three strains of cucumber mosaic virus affecting bell pepper in the everglades area of South Florida. Phytopathology 47: 145-150
30 Montasser, M., Tousignant, M. and Kaper, J. 1991. Satellite mediated protection of tomato against cucumber mosaic virus: I. Greenhouse experiments and simulated epidemic conditions in the field. Plant Dis. 75:86-92   DOI
31 Kaper, J. and Tousignant, M. 1984. Viral satellite: Parasitic nucleic acids capable of modulating disease expression. Endeavour N.S. 8:194-200   DOI   ScienceOn
32 Kaper, J. M., Tousignant, M. E. and Lot, H. 1976. A low molecular weight replicating RNA associated with a divided genome plant virus: Defective or satellite RNA? Biochem. Biophys. Res. Comun. 72:1237-1243
33 White, J. and Kaper, J. 1989. A simple method for detection of viral satellite RNAs in small plant tissue samples. J. Virol. Methods 23:83-94   DOI   ScienceOn
34 Douine, L, Quiot, J. B., Marchoux G and Archange, P. 1979. Recensement des especes vegetales sensibles au virus de la mosaique du conocombre (CMV). Etudes bibliographique. Ann. Phytopathol. 11:439