• Animal Bioscience
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• 제34권12호
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• pp.1981-1986
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• 2021

Objective: This study aimed to investigate the effect of Salvia miltiorrhiza (S.m.) aerial parts as an alternative ingredient on growth performance, nutrient digestibility, and digestive enzymes in growing rabbits. Methods: Treatments included five tested diets: a control (basal diet), antibiotic (basal diet+enramycin at 5 mg/kg), and S.m. aerial parts powder added at 3.0%, 6.0%, and 9.0% of feed using 300 growing rabbits. Results: The diets with S.m. aerial parts addition at 9.0% decreased (p<0.05) feed/gain compared to the control, but there were no differences in feed intake and body weight gain. In contrast with the control, the addition of antibiotic increased (p<0.05) digestibility of dry matter, crude protein, energy, fiber, and ash. The herb addition did not cause differences in the digestibility of most nutrients compared to the antibiotic, but fiber digestibility of the herb at 6.0% and 9.0% was lower (p<0.05) than that of the antibiotic. Moreover, the antibiotic and the herb also similarly increased (p<0.05) the activities of duodenal α-amylase, maltase, lipase, and trypsin, compared to the control, and the herb at 6.0% and 9.0% showed a greater (p<0.05) activity of elastase than the dose 3.0%. Conclusion: The obtained data indicate that S.m. aerial parts can be a potential forage in rabbit's diet at 9.0% with a beneficial regulation on nutrition and digestion.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1275-1285
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• 2021

The objective of the present experiment was to investigate the effect of dietary palm kernel meal (PKM) and β-xylanase supplementation on productive performance, egg quality, fatty liver incidence, and excreta characteristics in laying hens. A total of 320 Hy-Line Brown laying hens (33 weeks of age) were allotted to 1 of 4 treatments with 8 replicates in a feeding trial. Each replicate consisted of 10 consecutive cages with 1 hen per cage. The corn-soybean meal-based control diet was prepared. Additional diet was prepared by including 10% of PKM in the control diet with a partial replacement of corn, soybean meal, and animal fat. In addition, 0.025% β-xylanase was supplemented at the expense of celite to those 2 diets to produce 4 treatment diets in a 2 × 2 factorial arrangement. All hens were provided the diet and water ad libitum for 8 weeks. Results indicated no significant interactions between inclusion of dietary PKM and β-xylanase for all measurements; therefore, the main effects were mainly discussed. Hens fed diets containing 10% PKM had greater (p < 0.05) feed intake and yolk color than those fed diets containing no PKM. However, dietary PKM did not influence fatty liver incidence and excreta characteristics. Dietary β-xylanase supplementation had no effects on all measurements, regardless of inclusion of PKM. In conclusion, PKM can be a potential feed ingredient for laying hens at the inclusion of 10% in the diet. It appears that dietary β-xylanase used in the current experiment has little effect on layer productivity, regardless of inclusion of 10% PKM in the diet.

• 한국수산과학회지
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• 제52권4호
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• pp.366-373
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• 2019

The objective of this study was to investigate the utilization of diacylglycerol (DAG) as a new dietary ingredient replacing fish oil in feed for juvenile olive flounder Paralichthys olivaceus. Fish oil based control diet (CON) was prepared and four other diets were formulated by replacing 50% of the fish oil in CON with one of five DAG: DAGL (1,3-lauryl glycerol) or DAGP (1,3-palmityl glycerol) in low or high concentrations (designated as DAGLL, DAGLH, DAGPL and DAGPH). Another diet was prepared replacing 100% of the fish oil in CON with a 1:1 mixture (DAGLP) of DAGL and DAGP. Olive flounder (13.4 g) were fed to apparent satiation, twice a day, for 12 weeks. Following the feeding trials, no significant differences were observed in growth performance, blood parameters and non-specific immune responses between CON and any of the DAG groups. Polyunsaturated fatty acid levels were not significantly affected by the inclusion of DAGs. Thus, DAGL or DAGP could be used to replace up to 50% of fish oil in fish feed without reducing growth performance, health or innate immunity. The replacement of up to 100% of dietary fish oil in olive flounder feed by DAGLP is also feasible.

• Asian-Australasian Journal of Animal Sciences
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• 제32권6호
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• pp.849-855
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• 2019

Objective: This study was conducted to compare growth performance, nutrient digestibility and meat quality of broilers fed a genetically modified organism (GMO) diet or a non-GMO diet. Methods: A total of 840 broilers with an initial body weight of 43.03 g per chick were randomly allocated into 1 of the following 2 dietary treatments lasted for 32 days (15 broilers per pen with 28 replicates per treatment): i) Trt 1, GMO maize-soybean meal based diet; ii) Trt 2, non-GMO maize soybean meal based diet. Both diets were maize-soybean meal diets. The GMO qualitative analysis, proximate analysis and amino acid analysis of the feed ingredient samples were carried out. Diets were formulated based on a nutrient matrix derived from analysis results. Growth performance was measured on day 0, 7, 17, and 32. And all other response criteria were measured on day 32. Results: The analysis results showed that the total Lys, Met, Thr of non-GMO grains were lower than that of GMO grains, the protein content of GMO soybean meal was higher than that of non-GMO soybean meal. Feed intake and feed conversion rate (FCR) were greater (p<0.05) in broilers provided with non-GMO diet than that of the GMO group from d 17 to 32. A decrease in FCR was observed in birds fed the GMO diet through the entire experiment (p<0.05). No significant impacts on blood profile, meat quality and nutrient digestibility were found in response to dietary treatments throughout the experimental period (p>0.05). Conclusion: These results indicated that non-GMO diet showed a negative effect on growth performance but nutrient digestibility, blood profile, carcass weight and meat quality were not affected by non-GMO diets.

• Asian-Australasian Journal of Animal Sciences
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• 제32권7호
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• pp.1007-1014
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• 2019

Objective: This study was conducted to evaluate the fermentation characteristics under low mesophilic temperature of spent instant coffee ground (SICG) and to estimate the effect of fermented SICG (FSICG) as alternative feed ingredient on milk productivity of dairy cows. Methods: In the fermentation trial, fermentation of SICG was performed to investigate changes in characteristics using the microbial mixture (Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis = 1:1:1) for 21 days at $20^{\circ}C$ under anaerobic conditions. Molasses was added at 5% of dry mass. In the animal trial, eighteen Holstein Friesian cows were used to evaluate the nutritive value of the FSICG which was fermented for 14 days under the same condition as the fermentation trial. Results: In the fermentation trial, the dry matter (DM) and organic matter content linearly decreased with fermentation time (p<0.001 and p = 0.008, respectively). The acid detergent insoluble nitrogen content linearly decreased with fermentation time (p = 0.037). The microorganism counts linearly increased for Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis across fermentation time (p<0.001). In the animal trial, the DM intake of the control and FSICG treatment were not significantly different, as were milk yield, 4% fat corrected milk, fat-protein corrected milk, and feed to milk conversion content. Fat, protein, lactose, non-fat solids, milk urea nitrogen, and somatic cell counts were also not significantly different in milk composition between treatments. Conclusion: FSICG should be considered a sufficient substitute for cottonseed as a feed component, and 5% DM of a dietary FSICG level was appropriate for dairy cow diets.

• Fisheries and Aquatic Sciences
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• 제26권2호
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• pp.87-96
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• 2023

This study was designed to determine the apparent digestibility coefficients of soybean meal, soy protein concentrate (SPC), soy protein isolate (SPI), rapeseed meal (RSM), pea protein concentrate (PPC), wheat gluten meal (WGM) and wheat flour (WF) for olive flounder, Paralichthys olivaceus. A reference diet (RF) was formulated to meet the nutrient requirements of olive flounder with 1% chromic oxide (Cr₂O₃) as an inert indicator. Test diets were prepared to contain 70% RF and 30% of the test ingredient. Olive flounder, averaging 150 ± 8.0 g, was cultured in 400-L fiberglass tanks at a density of 25 fish per tank. Fecal collection columns were attached to each tank. Fecal samples were obtained from triplicate groups of fish for 4 weeks. Dry matter digestibility of SPC (75%) and WGM (76%) were significantly higher than the other test ingredients. Protein digestibility of SPC (85%), PPC (88%) and WGM (89%) were significantly higher than the other test ingredients, and protein digestibility of RSM (77%) and WF (76%) was lower than the other ingredients tested. Lipid digestibility of SPC (72%) and SPI (69%) were significantly higher than the other test ingredients. Energy digestibility of SPC (85%) and WGM (82%) were significantly higher than that of others tested ingredients. The availability of amino acids in WGM was generally higher than in other plant-feed ingredients. Therefore, SPC and WGM were seems to be efficient as potential protein sources for olive flounder compared to other tested ingredients. Overall, findings of the current study may assist in more efficient and economical formulation of diets using plant feed ingredients for olive flounder.

• Fisheries and Aquatic Sciences
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• 제25권11호
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• pp.537-548
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• 2022

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, energy and essential amino acids in animal-based feed ingredients were determined for olive flounder (Paralichthys olivaceus). A reference diet (RF) was formulated to contain 1.0% chromic oxide (Cr₂O₃) as an inert indicator. Nine test diets were formulated to contain RF and one of the feed ingredients (pollock meal [PM], jack mackerel meal [JMM], anchovy meal [AM], cod meal [CM], sardine meal [SM], sand eel meal [SEM], tuna meal [TM], meat meal [MM] and squid liver meal [SLM]) at a 7:3 ratio in each diet designated as PM, JMM, AM, CM, SM, SEM, TM, MM and SLM, respectively. Olive flounder, averaging 150 ± 8.0 g, were stocked at a density of 25 fish per tank in 400-L fiberglass tanks attached with fecal collection columns. Feces were collected from triplicate groups of fish one time a day for four weeks. Dry matter and crude protein ADCs of CM and SEM were significantly higher than the other tested ingredients. Lipid ADCs of JMM, CM and SEM were significantly higher than the other test ingredients. Energy ADCs of CM and SEM were significantly higher than that of the other tested ingredients. The availability of amino acids in CM was generally higher than the other animal protein sources. PM exhibited the lowest amino acid availability among the treatments. Interestingly, MM exhibited significantly higher nutrient digestibility than several marine-based ingredients. However, CM and SEM are seeming to be highly digestible and effective to use in olive flounder diet compared to the other tested ingredients. Overall, the results of this study provide information about the bioavailability of nutrients and energy in animal feedstuffs to apply when formulating cost-effective practical feeds for olive flounder.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1143-1150
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• 2003

This study was conducted to investigate in vitro methane production of feed ingredients and relationship between the content of crude nutrients and methane production. Feed ingredients (total 26) were grouped as grains (5 ingredients), brans and hulls (8), oil seed meals (9) roughages (3), and animal by-product (1) from their nutrient composition and their methane production protential were measured by in vitro gas test. Among the groups, the in vitro methane productions for both 6 and 24 h incubation were highest in grains, followed by brans and hulls, oil meals and roughages, animal byproducts. Within the group of grains, methane production from wheat flour was the highest, followed by wheat, corn, tapioca, and then oat. Within the brans and hulls, soybean hull showed the highest methane production and cotton seed hull, the lowest. Methane production from oil meals was lower compared with grains and brans and hulls, and in decreasing order production from canola meal was followed by soybean meal, coconut meal, and corn germ meal (p<0.01). Three ingredients were selected and the interactions among feed ingredients were evaluated for methane production. Correlation coefficient between measured and estimated values of the combinations were 0.91. Methane production from each feed ingredient was decreased with increasing amount of crude fiber (CF), protein (CP) and ether extract (EE), whereas positive relationship was noted with the concentrations of N-free extract (NFE). The multiple regression equation (n=134) for methane production and nutrient concentrations was as follows. Methane production (ml/0.2 g DM)=(0.032${\times}$CP)-(0.057${\times}$EE)-(0.012${\times}$CF)+(0.124${\times}$NFE) (p<0.01; $R^2$=0.929). Positive relationship was noted for CP and NFE and negative relationship for CF and EE. It seems possible to predict methane production potential from nutritional composition of the ingredients for their effective application on formulating less methane emitting rations.

• Asian-Australasian Journal of Animal Sciences
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• 제28권6호
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• pp.847-854
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• 2015

The objective of this study was to determine the effects of graded inclusions of wheat bran (0%, 9.65%, 48.25% wheat bran) and two growth stages (from 32.5 to 47.2 kg and 59.4 to 78.7 kg, respectively) on the apparent ileal digestibility (AID), apparent total tract digestibility (ATTD) and hindgut fermentation of nutrients and energy in growing pigs. Six light pigs (initial body weight [BW] $32.5{\pm}2.1kg$) and six heavy pigs (initial BW $59.4{\pm}3.2kg$) were surgically prepared with a T-cannula in the distal ileum. A difference method was used to calculate the nutrient and energy digestibility of wheat bran by means of comparison with a basal diet consisting of corn-soybean meal (0% wheat bran). Two additional diets were formulated by replacing 9.65% and 48.25% wheat bran by the basal diet, respectively. Each group of pigs was allotted to a $6{\times}3$ Youden square design, and pigs were fed to three experimental diets during three 11-d periods. Hindgut fermentation values were calculated as the differences between ATTD and AID values. For the wheat bran diets, the AID and ATTD of dry matter (DM), ash, organic matter (OM), carbohydrates (CHO), gross energy (GE), and digestible energy (DE) decreased with increasing inclusion levels of wheat bran (p<0.05). While only AID of CHO and ATTD of DM, ash, OM, CHO, GE, and DE content differed (p<0.05) when considering the BW effect. For the wheat bran ingredient, there was a wider variation effect (p<0.01) on the nutrient and energy digestibility of wheat bran in 9.65% inclusion level due to the coefficient of variation (CV) of the nutrient and energy digestibility being higher at 9.65% compared to 48.25% inclusion level of wheat bran. Digestible energy content of wheat bran at 48.25% inclusion level (4.8 and 6.7 MJ/kg of DM, respectively) fermented by hindgut was significantly higher (p<0.05) than that in 9.65% wheat bran inclusion level (2.56 and 2.12 MJ/kg of DM, respectively), which was also affected (p<0.05) by two growth stages. This increase in hindgut fermentation caused the difference in ileal DE (p<0.05) to disappear at total tract level. All in all, increasing wheat bran levels in diets negatively influences the digestibility of some nutrients in pigs, while it positively affects the DE fermentation in the hindgut.

• 한국가금학회지
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• 제10권1호
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• pp.53-59
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• 1983

본 시험은 분채취시간 및 Corn Starch, Glucose Mixture (Starch (50) ＋ Glucose (50))의 급이가 성웅계의 내인성에너지 (FEm＋UEe) 손실에 미치는 영향을 검토하고자 실시한 결과 다음과 같은 성적을 얻었다. 1. Period II(24-28hr)의 분배설량, 에너지량과 N 손실량은 Period I(0-24hr)의 경우보다 높았으나 유의차 (P＝.05)는 없었다. 2. 체중(X)과 분배설량, 에너지 및 N 손실량 (Y) 간에는 유의차 상관관계는 발견되지 않았는데 (P＞.05) 이는 체중이 내인성에너지 (FEm＋UEe) 손실에 영향하지 않는다는 사실을 뒷받침하는 것이었다. 3 Glucose, Mixture 급이구는 절식구에 비하여 분배설량, 에너지 및 N 손실량이 현저하게 감소하였다. (P＜.05). 4. Corn Starch 급이구의 분배지량, N 손실량은 절식구에 비해 유의적 (P＜.05)으로 감소했으나 내인성에너지 손실량은 두 구간 유의차가 없었다. (P＞.05) 이는 Corn Starch의 Apparent Digestibility가 Glucose, Mixture구 보다 낮았음을 지적하는 것이다. 5. 급이구의 N 손실량은 절식구에 비하여 현저히 감소한 사실은 탄수화물 급이가 절식동안 조직의 이화작용에 의한 에너지 손실의 일부를 보완하는 것으로 생각된다.