• Korean Journal of Plant Taxonomy
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• v.38 no.3
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• pp.197-222
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• 2008

A recently published Korean Flora, "The genera of vascular plants of Korea (GFK)", includes the descriptions and keys for 217 families, 1,044 genera, and 3,209 species of Korean vascular plants. We reclassified these taxa according to the recent APG classification system, which resulted in 64 orders, 204 families, 1,044 genera and 3,209 species. Twenty-two families from the GFK were abandoned because of changes to the familial delimitations in the APG system. In contrast, the number of families in the Liliaceous group was increased. The Liliaceae in the GFK included 31 genera and 109 species. These taxa are now assigned to 10 families in four different orders including Liliales, Asparagales, Alismatales, and Dioscoreales because of the drastic changes to the monocot classification system in the past 20 years. In addition, the family name of the Aucubaceae was changed to Garryaceae. As a result, the number of families in the GFK has been reduced to 204. The results were summarized in four tables and two figures at the levels of unofficial higher taxonomic hierarchies, orders, families and genera. This new information can provide a guidelines for selecting the phylogenetic analysis unit for the Korean tree of life (KTOL) project. Futhermore, the updated classification system also provides an important summarization for the systematic community for placing the Korean flora in a modern phylogenetic context.

• Journal of Life Science
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• v.13 no.6
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• pp.764-774
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• 2003

The edible plants sold at the market in Busan were classified into 8 divisions, 11 classes, 46 orders, 67 families, 156 genera, 183 species and 45 varieties, and a total of 228 kinds of plants were included in them. Among the 228 kinds of edible plants, Angiospermae were the highest number of 202 kinds (88.60%), and next came Fungi of 9 kinds (3.95%), Phaeophyta of 5 kinds (2.19%), Rhodophyta of 4 kinds (1.75%), Pteridophyta of 3 kinds (1.32%) Gymnospermae of 2 kinds (0.88%), Chlorophyta of 2 kinds (0.88%), and Lichenes of 1 kind (0.44%) in order In the taxon of family, Cruciferae, Rosaceae, Leguminoceae, Cucurbitaceae, Umbelliferae, Compositae, Liliaceae, and Gramineae include many species. In the taxon of genus, Brassica(14 species), Prunus(9), Lactuca(9), Allium(7), Citrus(5), Cucumis(4), Cichorium(4) in order contain many species. Among tile edible plants, Chamjuknamu(Cedrela sinensis) is wrongly named Gajuknamu(Ailanthns altissima). And there are others which are called in a wrong way: Endive is wrongly called Chicory, Saeneutaribeoseot, Saesongi and Padeudeknamul(Japanese hornwort), Chamnamul. Gyul and Milgam, Geumgam and Gamgyul, Banana and Pacho, Paprika and Pimang(Pimento) are the names of the same plants. The number of the kinds of edible plants is different in each season. Fall has the most edible plants, and it is followed by spring, summer and winter. The edible parts of plants which are sold at the market are leaves, young sprouts, fruits, grains, roots, stems and petioles.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.584-592
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• 2004

A thermostable $\beta$-glycosidase gene, tfi $\beta$-gly, was cloned from the genomic library of Thermus filiformis Wai33 A1. ifi $\beta$-gly consists of 1,296 bp nucleotide sequence and encodes a polypeptide of 431 amino acids. It shares a strong amino acid sequence similarity with the $\beta$-glycosidases from other Thermus spp. belonging to the glycosyl hydrolase family 1. In the present study, the enzyme was overexpressed in Escherichia coli BL21 (DE3) using the pET21b(+) vector system. The recombinant enzyme was purified to homogeneity by heat treatment and a $Ni^{2+}$-affinity chromatography. Polyacrylamide gel electrophoresis (PAGE) showed that the recombinant Tfi $\beta$-glycosidase was a monomeric form with molecular mass of 49 kDa. The temperature and pH range for optimal activity of the purified enzyme were 80- $90^{\circ}C$ and 5.0-6.0, respectively. Ninety-three percent of the enzyme activity was remained at $70^{\circ}C$ after 12 h, and its half-life at $80^{\circ}C$ was 6 h, indicating that Tfi $\beta$-glycosidase is highly thermostable. Based on its K_m$, or $K_{cat}K_m$, ratio, Tfi $\beta$-glycosidase appeared to have higher affinity for $\beta$-D-glucoside than for $\beta$-D-galactoside, however, $K_{cat} for \beta$-D-galactoside was much higher than that for $\beta$-D-glucoside. The activity for lactose hydrolysis was proportionally increased at $70^{\circ}C$ and pH 7.0 without substrate inhibition until reaching 250 mM lactose concentration. The specific activity of Tfi TEX>$\beta$-glycosidase on 138 mM lactose at $70{^\circ}C$ and pH 7.0 was 134.9 U/mg. Consequently, this newly cloned enzyme appears to have a valuable advantage of conducting biotechnological processes at elevated temperature during milk pasteurization in the production of low-lactose milk.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.402-410
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• 2013

In this study, to identify ichthyofauna of the Seomjin River, which has no barrage at estuary, and to estimate effect of estuary barrage, fish sampling was conducted at 16 study sites in mainstream of the Seomjin River from 2010 to 2012. A total of 54 species classified into 17 families were collected. Cyprinidae was the dominant family and Zacco platypus (relative abundance, RA: 47.8%) was identified as the dominant species. In total, 17 Korean endemic species (31.5%) were collected, and it is higher than the average endemic rate of Korean peninsula (22.5~25.9%). Although the relative abundance of exotic species (1.1%) was low, it is higher than the past studies (2002: 0.0%, 2009: 0.4%). Dominance index of freshwater region (St.4~St.16) was relatively higher than estuary (St.1~St.3), because Z. platypus showed high relative abundance at freshwater region. But diversity and evenness index showed contradictory results, as estuary was higher than freshwater region. The ratios of sea fish, peripheral freshwater fish and economic fish, and diversity index are higher than the Nakdong River, the Yeongsan River and the Geum River. In conclusion, to conserve the value of the Seomjin River ichthyofauna, continuous monitoring is necessary.

• Korean Journal of Microbiology
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• v.42 no.2
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• pp.142-148
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• 2006

In order to develop chitosanase for the production of chitosan oligosaccharides, a chitosanase-producing bacterium was isolated from the traditional fermented soybean, Meju, and identified as Bacillus amyloliquefaciene MJ-1. The cloned chitosanase gene, 825 bp in size, encoded a single peptide of 274 amino acids with a estimated molecular mass of 30.9 kDa. The deduced amino acid sequence showed significant homology with microbial chitosanases. The recombinant chitosanase was expressed in Escherichia coli upon induction with isopropyl-D-thiogalactopyranoside, and purified using $Ni^{2+}-NTA$ agarose column chromatography. The maximal activity of the recombinant chitosanase is at pH 5.0 and $60^{\circ}C$. The recombinant chitosanase is stable between pH 5.0 and pH 7.0 at $37^{\circ}C$ for 30 min, and more than 75% of the activity still remain at $80^{\circ}C$ for 30 min incubation.

• Korean Journal of Social Welfare Studies
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• v.43 no.1
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• pp.365-388
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• 2012

This study examined the effect of bilingual bicultural home environment and mother's command of Korean language on their participation in child rearing as well as on children's developmental outcomes. We carried out Analysis of Covariance Structure using the 1st year(2011) data of Gyeonggi Education Welfare Panel, which consists of 459 matched pairs of immigrant women and their 5th~6th grade children living in Gyeonggi-do. The results indicated that when the immigrant mother has good command of Korean language and the children are exposed to their maternal culture and language, mothers tend to actively participate in child rearing and this in turn has positive effects on developmental outcomes of their children. These results demonstrate that Korean language programs targeting immigrant women by marriage are important not only for assisting their employment and adaptation but also for enhancing parenting behaviors in multicultural families. Moreover these findings suggest that we need to understand the bilingual bicultural home environment in these families as a significant resource that contribute to their children's developmental outcomes and not as a risk factor to their adaptation, as it has frequently been viewed.

• Journal of Life Science
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• v.31 no.4
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• pp.377-384
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• 2021

Researching the soil fungal community is important to understand the interaction between fungi and living plants. However, too few studies have examined the soil fungal community and their interactions with plants. Rhododendron mucronulatum, commonly known as Korean rosebay, is an important forest resource that has aesthetic, ecological, and potential pharmacological values. We used a pyrosequencing method to analyze the characteristics of fungal communities from R. mucronulatum soil samples from Biseul mountain county park, which is one of the famous places for large R. mucronulatum colonies in South Korea. We collected soil core samples in February and August at three sites in the Biseul Mountain County Park, taking into consideration the regional and seasonal conditions. We obtained 454,157 validated reads after pyrosequencing all six samples. The fungal communities from the first observation spot in August had the richest species diversity among the samples. Basidiomycota, Ascomycota, and Mortierellomycota were major phyla in the samples. Agaricales_f, Mortierellaceae, and Clavariaceae were major families in the samples. The genus Mortierella was the most dominant in all six samples. Overall, 19 genera could be associated with R. mucronulatum. Sample 1 had 109 genera in sample 1, sample 2 had 111 genera, and sample 3 had 112 genera that were uniquely identified. The samples collected in August had 28 identified genera, that existed only in summer samples, indicating a weather effect. This study can be used as basic research to understand the relationship between soil fungi and plants.

• Journal of Life Science
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• v.28 no.11
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• pp.1332-1338
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• 2018

Activated microglia, induced by various pathogens, protect neurons and maintain homeostasis of the central nervous system (CNS). However, severe activation causes neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease because of the secretion of various neurotoxic molecules, such as nitric oxide (NO), prostaglandin (PG), and pro-inflammatory cytokines. Because chronic microglial activation endangers neuronal survival, negative regulators of microglial activation have been identified as potential therapeutic candidates for treatment of many neurological diseases. One potential source of these regulators is Locusta migratoria, a grasshopper of the Acrididae, usually 4-6 cm in size, belonging to the family of large insects in Acrididae. This grasshopper is an edible insect resource that can be consumed by humans as protein source or used for animal feed. The aim of the present study was to examine the inhibitory effects of a L. migratoria ethanol extract (LME) on the production of inflammatory mediators in LPS-stimulated BV-2 microglia cells. The extract significantly inhibited the NO, iNOS, COX-2, and pro-inflammatory cytokine ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$) levels in BV-2 microglia cell. Because the inhibition of microglial activation may be an effective solution for treating brain disorders like Alzheimer's and Parkinson's diseases, these results suggest that LME may be a potential therapeutic agent for the treatment of brain disorders induced by neuroinflammation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.4
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• pp.366-375
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• 2018

Evapotranspiration (ET) information is needed for many applications in agricultural and environmental resource management because crop yields, plant growth and physiological characteristics are primarily water limited. This study was conducted to evaluate the diurnal change of ET using electronic weighing lysimeter and to determine whether the ET of Cnidium officinale Makino could be manipulated through meteorological factors such as solar radiation, windy conditions and air temperature etc., Pot has a diameter of 35 cm and an height of 38 cm. A disturbed soil of sandy loam (coarse, mixed, mesic family of Dystric Fluventic Eutroudepts) within lysimeter has a mass of approximately 40.0 kg. In 2017, 10-minute recordings of data were used for measuring actual ET, and also evaluating a relationship between ET and meteorological factors during from 2 Aug. to 6 Aug. The maximum daily ET of Cnidium officinale was $44.04{\pm}3.949g$ per hour in lysimetric measurements. Diurnal changes of ET was highly correlated with solar radiation ($r^2=0.7778$) and followed by wind speed ($r^2=0.6400$). But on the other hand, air temperature was not consistent with ET ($r^2=0.2260$). This results imply that ET of Cnidium officinaele seems to be mainly governed by radiation energy in clear days, and approximately 40% of solar radiation is likely to be converted into ET. Therefore, weighing lysimeter can be used to accurately estimate actual ET and is expected to attract a great deal of attention to reliable application of water management in agriculture.

• Journal of Korean Society of Rural Planning
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• v.24 no.4
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• pp.121-134
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• 2018

By using the raw data of the 2015 Agriculture, Forestry and Fishery Consensus that surveyed the farmers engaged in agriculture, forestry and fishery in 2015, this study investigated the effects of the characteristics of the farms in Gangwon-do on the utilization of the digital devices, which was classified into computers usage only, smartphones usage only, both computers and smartphones usage, and no digital device usage. This study used the Multinomial Logit Model for the above purpose. Moreover, the IIA(Independence of Irrelevant Alternatives) method of the Multinomial Logit Model was analyzed to be suitable. Upon the result of the analysis, when all the other variables were constant in the average, the probability of choosing both computers and smartphones increased by 0.02% as the family member living together with the farm owner increased by 1 person. In addition, the farms with agricultural machineries have 0.03% increased probability of using smartphones to sell their agricultural products, when compared with the farms without agricultural machinery. Moreover, for the farms with high sales profit of the agricultural products, the probability of using computers and the probability of using smartphones increased by 0.04% and 0.01%, respectively, and the probability of using both computers and smartphones increased by 0.02%. On the other hand, for the farms affiliated with the agricultural organization, the probability of using both computers and smartphones increased by 0.17%, and the probability of using only smartphones and the probability of using only computers increased by 0.16% and 0.15%, respectively. In case of selling to the National Agricultural Cooperative Federation(NACF), the probability of using smartphones increased by 0.11% if all other variables were to be constant in the average. In particular, the farms participating in the agriculture-related industries have 1.09% increased probability of using computers, have 0.92% increased probability of using smartphones, and 0.85% increased probability of using both computers and smartphones. Therefore, the implications suggested based on the results of the analysis are as follows. First, the farms' choices of the digital devices are made independently. Hence, the future agricultural industry business platform establishment should necessarily consider the types of farms' choices of the digital devices in the future. Second, since the usage of digital devices has a generally positive influence on the farm household income, digital education to improve farmers' ability to use the digital devices should be urgently provided in order to improve the farm household income in the farms in Gangwon-do. Third, because the digital devices used to sell agricultural products differ depending on the age of the farm owner, it would be desirable to target the younger generation of the farm owners rather than establishing the agricultural industry business platform for the farms in the aged farm villages. Fourth, it would be advisable for the future agricultural business platform to consider the characteristics of agricultural products sales source.