• Title/Summary/Keyword: f/2 media

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Toxicity Effects of Copper on the Physiological Responses of Anabaena flos-aquae (Cyanophyceae) (구리독성이 Anabaena flos-aquae의 생리적 변화에 미치는 영향)

  • Ryu, Ji-Won;Choi, Eun-Joo;Rhie, Ki-Tae
    • Environmental Analysis Health and Toxicology
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    • v.23 no.2
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    • pp.101-112
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    • 2008
  • Effects of various concentrations of copper on growth change of blue-greenalgae Anabaena flos-aquae were studied. There was significant differences among cultures treated with various concentrations of copper in growth of algae with parameters of cell numbers, specific growth rate (SGR) and chlorophyll contents. Algal growth and SGR were inhibited on by effect of various concentrations of copper more than without copper (ANOVA, F=34.69 p<0.001, F=114.89, p<0.001). The SGRs of various concentrations of copper in media were higher than without copper on 8 days after copper treated. The mean of chlorophyll contents was 1.978 ${\mu}g{\cdot}mL^{-1}$ and 1.648 ${\mu}g{\cdot}mL^{-1}$, respectively, while those of algae in culture without copper was 3.179 ${\mu}g{\cdot}mL^{-1}$ (ANOVA, F=153.74, p<0.001). The cellular morphology was different between media of which copper treated and without copper. The colony of algae in media which copper treated was shorter than without copper. Effects of various concentrations of copper on growth change of blue-green-algae Anabaena flos-aquae occured variety changes of parameters of cell numbers, specific growth rate (SGR), chlorophyll contents and cellular morphology on growth of algae.

Protoplast Culture by Electrofusion of Protoplasts from Solanum sisymbriifolium and Other Solanum Species

  • Kim Hag-Hyun;Jung Hye-Jin;Shin Un-Dong
    • Korean Journal of Plant Resources
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    • v.19 no.3
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    • pp.422-426
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    • 2006
  • This research was conducted to get the basic materials necessary to obtain the somatic hybrid plant between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium). Regarding the formation of colony from the protoplast in S. sisymbriifolium, S. integrifolium and the fused protoplast mixture; for the S. sisymbriifolium, a colony was observed in F medium(Kao medium containing $5.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ 2,4-D and $1.0mg{\cdot}L^{-1}$ BA); and for the S. integrifolium, in G medium (a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ kinetin) respectively. In mixed cultured protoplast after electriofusion treatment, the cell division and colony formation were observed in both media F and G. For the shoot and root formation rate, there was no difference between the parent of each breed and mixed protoplast regardless of the medium. In the fused protoplast mixture of S. sisymbriifolium and S. toxicarium, a colony formation was also observed in both media F and H(a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{\cdot}L^{-1}\;NAA,\;1.0mg{\cdot}L^{-1}$ kinetin); and there was no difference in the shoot and root formation rate between the parent and the mixed protoplast.

The Effect of Mass Media on Women's Clothing Image, Make-up Image and Hair Image (매스미디어가 여성의 의복과 메이크업 및 헤어이미지에 미치는 영향)

  • Choi, Su-Koung
    • Journal of the Korea Fashion and Costume Design Association
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    • v.13 no.2
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    • pp.35-46
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    • 2011
  • The purpose of this study was to investigate the effect of mass media on women's clothing image, make-up image, and hair image. The subjects of the study were 306 women who lived in Gyeongnam area. Data were collected during June in 2009. Statistical analysis used in this study were frequency, F-test, t-test, Duncan test, factor analysis, Cronbach's ${\alpha}$, correlation coefficient, and multiple regression. The results of this study were as follows. The mass media according to the demographic characteristic of women showed significant difference. Three factors of clothing image were titled as elegance, youth, and visibility. Three factors of make-up image were titled as modern, romanticism, and mediocrity. Four factors of hair image were titled as attraction, individuality, gentleness, and cuteness. The mass media resulted in correlation with the clothing image, make-up image, and hair image. The mass media had a influence on the clothing image, make-up image, and hair image. The study results are highly expected to be used as useful sources in one's image formation and a marketing plan.

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Identification of QTLs controlling somatic embryogenesis using RI population of cultivar ${\times}$ weedy soybean

  • Choi, Pilson;Mano, Yoshiro;Ishikawa, Atsuko;Odashima, Masashi;Umezawa, Taishi;Fujimura, Tatsuhito;Takahata, Yoshihito;Komatsuda, Takao
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.23-27
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    • 2010
  • Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

Heart Response Effect by 1/f Fluctuation Sounds for Emotional Labor on Employee (1/f 수준 별 음악 자극이 감정 노동 종사자의 심장 반응에 미치는 효과)

  • Jeon, Byung-Mu;Whang, Min-Cheol
    • Science of Emotion and Sensibility
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    • v.18 no.3
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    • pp.63-70
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    • 2015
  • This study identified heart response of participants while listening to sounds which have 1/f fluctuations with exponent ${\alpha}$ gradient. The participants were engaged in emotional stress work. Prior studies related to 1/f fluctuation sound have reported that sound source can alleviate psychological and physiological state of users. Subjects of this study were exposed to sound with three levels of ${\alpha}$ gradient. Heart response of subjects were measured with Photoplethysmography(PPG) sensor simultaneously. The dependent variables of this study were beat per minute(BPM), very low frequency percent of pulse rate variability (VLF percent), the standard deviation of all normal RR intervals (SDNN), and high frequency power(HF power). Subject showed arousal response when exposed to sound with exponent ${\alpha}$ gradient of 3 whereas the sound with exponent ${\alpha}$ gradient of 1 and 2 resulted in relax effect. The characteristic of 1/f fluctuation sounds can be applied to alleviate stress for employers under emotional labor.

Detecting Fake Job Recruitment with a Machine Learning Approach (머신 러닝 접근 방식을 통한 가짜 채용 탐지)

  • Taghiyev Ilkin;Jae Heung Lee
    • Smart Media Journal
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    • v.12 no.2
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    • pp.36-41
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    • 2023
  • With the advent of applicant tracking systems, online recruitment has become more popular, and recruitment fraud has become a serious problem. This research aims to develop a reliable model to detect recruitment fraud in online recruitment environments to reduce cost losses and enhance privacy. The main contribution of this paper is to provide an automated methodology that leverages insights gained from exploratory analysis of data to distinguish which job postings are fraudulent and which are legitimate. Using EMSCAD, a recruitment fraud dataset provided by Kaggle, we trained and evaluated various single-classifier and ensemble-classifier-based machine learning models, and found that the ensemble classifier, the random forest classifier, performed best with an accuracy of 98.67% and an F1 score of 0.81.

Deep Learning for Weeds' Growth Point Detection based on U-Net

  • Arsa, Dewa Made Sri;Lee, Jonghoon;Won, Okjae;Kim, Hyongsuk
    • Smart Media Journal
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    • v.11 no.7
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    • pp.94-103
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    • 2022
  • Weeds bring disadvantages to crops since they can damage them, and a clean treatment with less pollution and contamination should be developed. Artificial intelligence gives new hope to agriculture to achieve smart farming. This study delivers an automated weeds growth point detection using deep learning. This study proposes a combination of semantic graphics for generating data annotation and U-Net with pre-trained deep learning as a backbone for locating the growth point of the weeds on the given field scene. The dataset was collected from an actual field. We measured the intersection over union, f1-score, precision, and recall to evaluate our method. Moreover, Mobilenet V2 was chosen as the backbone and compared with Resnet 34. The results showed that the proposed method was accurate enough to detect the growth point and handle the brightness variation. The best performance was achieved by Mobilenet V2 as a backbone with IoU 96.81%, precision 97.77%, recall 98.97%, and f1-score 97.30%.

Effect of pH, Saturated Oxygen, and Back-flushing Media in Hybrid Water Treatment of Tubular Ceramic MF and Photocatalyst-loaded PES Beads (관형 세라믹 정밀여과와 광촉매 첨가 PES 구의 혼성수처리에서 pH 및 포화산소, 역세척 매체의 영향)

  • Hong, Sung Taek;Park, Jin Yong
    • Membrane Journal
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    • v.24 no.2
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    • pp.123-135
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    • 2014
  • The effects of pH, saturated oxygen, and back-flushing media were investigated in hybrid process of tubular ceramic microfiltration and $TiO_2$ photocatalyst-loaded PES (polyethersulfone) beads for advanced drinking water treatment, and compared results of water, nitrogen, or oxygen back-flushing in the viewpoints of membrane fouling resistance ($R_f$), permeate flux (J) and total treated water ($V_T$). $R_f$ decreased, and J and $V_T$ increased as decreasing pH. Turbidity treatment efficiencies were similar at water or nitrogen back-flushing independent of pH, but DOM (dissolved organic matter) treatment efficiency did not have a trend at water back-flushing. $R_f$ at NBF (no back-flushing) with SO (saturated oxygen) was the lower than that at NBF without SO. Also, the DOM treatment efficiency at NBF with SO was the lower than that at NBF without SO. It happened because OH radicals produced by reaction of SO and photocatalyst could dilute with water inside the module. The DOM treatment efficiency of gas back-flushing showed the larger than that of water back-flushing at back-flushig period 10 min. It proved that the adsorption or photo-oxidation of PES beads could be activated by the more effective bead-cleaning of gas back-flushing than water back-flushing.

Protoplast Isolation and Differentiation of Marine Green Alga Monostroma nitidum (해산 녹조류 참홑파래, Monostroma nitidum의 원형질체 분리와 분화)

  • CHO Yong Chul;GONG Yong Gun;YOON Jang-Taek;SUN Sang-Mi;CHUNG Gyu-Hwa
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.1
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    • pp.117-120
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    • 1999
  • High yields of protoplasts were obtained following enzymatic digestion of the vagetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing $4\%$ Cellulase R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power produced $4.41\times10^6$ protoplasts per 300 mg of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between $13\~33\mu$m in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.

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Mycological Characteristics of Fusarium solani f. sp. pisi Isolated from Pea, Ginseng and Soybean in Korea

  • Jung, Yong-Sung;Kim, Young-Tae;Yoo, Sung-Joon;Kim, Hong-Gi
    • The Plant Pathology Journal
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    • v.15 no.1
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    • pp.44-47
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    • 1999
  • Fungal isolates belong to Fusarium solani (perfect stage : Nectria haematococca) were isolated form pea, ginseng roots and soybean during 1995 and 1996 in Korea. Thirty-five isolates were identified as F. solani f. sp. pisi based on the morphological characteristics and their pathogenicity. Microconidia formed on the long conidiophore were ovoid or oblong sizing 5~14 x 2.5~5.0 $\mu\textrm{m}$. Macroconidia were formed on carnation leaf agar media with 4.8~5.3 x 32.0~40.7 $\mu\textrm{m}$ in size. They belonged to $\beta$-type since their 3-septate macroconidia were smaller than $5\mu\textrm{m}$ in width. Chlamydospores were smooth- or rough- walled and formed in terminal or lateral branches of hyphae, intercalary, or in chains. Most isolates were highly virulent to pea seedlings, producing dark brown or rot lesions. This is the first report of f. solani f. sp. pisi being pathogenic to pea in Korea.

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