Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of QTLs controlling somatic embryogenesis using RI population of cultivar ${\times}$ weedy soybean

  • Received : 2009.04.23
  • Accepted : 2009.10.19
  • Published : 2010.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Quantitative trait loci (QTLs) controlling ability of somatic embryogenesis were identified in soybean. A frame map with 204-point markers was developed using an RI population consisting of 117 $F_{11}$ lines derived from a cross between cultivar 'Keburi' and a weedy soybean 'Masshokutou Kou 502'. The parents differed greatly in their abilities of somatic embryogenesis using immature cotyledons as explants. The ability of somatic embryogenesis was evaluated in five different experiments: the $F_{11}$ (evaluated in 1998) and $F_{15}$ (2002) generations cultured on basal media supplemented with $40\;mg\;l^{-1}$ 2,4-D (2,4-D1998 and 2,4-D2002), $F_{14}$ (2001) generation on medium with $40\;mg\;l^{-1}$ 2,4-D and high sucrose concentration [2,4-D2001 ($30\;g\;l^{-1}$ sucrose)], and the $F_{11}$ (1998) and $F_{12}$ (1999) generations on medium with $10\;mg\;l^{-1}$ NAA (NAA1998 and NAA1999). The RILs showed wide and continuous variations in each of the five experiments. In the composite interval mapping analysis, 2 QTLs were found in group 8 (D1b + W, LOD = 5.42, $r^2$ = 37.5) in the experiment of 2,4-D1998 and in group 6 (C2, LOD = 6.03, $r^2$ = 26.0) in the experiment of 2,4-D2001 (high concentration sucrose). In both QTLs, alleles of 'Masshokutou Kou 502' with high ability of somatic embryogenesis contributed to the QTLs. For the other three experiments, no QTL was detected in the criteria of LOD >3.0, suggesting the presence of minor genes.

Keywords

References

  1. Armstrong CL, Romero-Severson J, Hodges TK (1992) Improved tissue-culture response of an elite maize inbred through backcross breeding, and identification of chromosomal regions important for regeneration by RFLP analysis. Theor Appl Genet 84:755-762
  2. Basten CJ, Weir BS, Zeng Z-B (2000) QTL cartographer, version 1.14. Department of Statistics, North Carolina State University, Raleigh
  3. Ben Amer IM, Korzun V, Worland AJ, Borner A (1997) Genetic mapping of QTLs controlling tissue-culture response on chromosome 2B of wheat (Triticum aesativum L.) in relation to major genes and RFLP markers. Theor Appl Genet 94:1047-1052 https://doi.org/10.1007/s001220050513
  4. Berrios EF, Sarrafi A, Fabre F, Alibert G (2000) Genotypic variation and chromosomal location of QTLs for somatic embryogenesis revealed by epidermal layers culture of recombinant inbred lines in the sunflower (Helianthus annus L.). Theor Appl Genet 101:1307-1312
  5. Choi PS, Mano Y, Ishikawa A, Odashima M, Umezawa T, Fujimura T, Takahata Y, Komatsuda T (2003) Construction of a highdensity AFLP and SSR map using recombinant inbred lines of cultivated ${\times}$ weedy soybean. Breed Sci 53:335-344 https://doi.org/10.1270/jsbbs.53.335
  6. Endo M, Komatsuda T, Takahata Y, Kaizuma N (1998) Parent-offspring relationship in somatic embryogenesis from immature soybean embryo. Breed Sci 48(Suppl 2):269
  7. Fukuda Y (1933) Cytogenetic studies of wild and cultivated Manchurian soybeans (Glycine L.). Jpn J Bot 6:489-506
  8. Ito M, Komatsuda T, Takahata Y, Kaizuma N (1999) Genotype ${\times}$ sucrose interactions for 2, 4-D-induced somatic embryogenesis in soybean (Glycine max L.). Plant Biotechnol 16:419-421 https://doi.org/10.5511/plantbiotechnology.16.419
  9. Komatsuda T, Ko S (1990) Screening of soybean (Glycine max L. Merrill) genotypes for somatic embryo production from immature embryos. Jpn J Breed 40:249-251 https://doi.org/10.1270/jsbbs1951.40.249
  10. Komatsuda T, Ohyama K (1988) Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max. Theor Appl Genet 75:695-700
  11. Komatsuda T, Kaneko K, Oka S (1991) Genotype ${\times}$ sucrose interactions for somatic embryogenesis in soybean. Crop Sci 31:333-337 https://doi.org/10.2135/cropsci1991.0011183X003100020023x
  12. Komatsuda T, Annaka T, Oka S (1993) Genetic mapping of quantitative trait locus (QTL) that enhances the shoot differentiation rate in Hordeum vulgare L. Theor Appl Genet 86:713-720
  13. Komatsuda T, Nakamura I, Takaiwa F, Oka S (1998) Development of STS markers closely linked to the vrs1 locus in barley, Hordeum vulgare. Genome 41:680-685
  14. Kwon YS, Kim KM, Eun MY, Sohn JK (2001) Quantitative trail loci mapping associated with plant regeneration ability from seed derived callus in rice (Oryza sativa L.). Mol Cell 11:64-67
  15. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174-181 https://doi.org/10.1016/0888-7543(87)90010-3
  16. Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0, 3rd. Whitehead Institute Technical Report
  17. Mano Y, Komatsuda T (2002) Identification of QTL controlling tissue-culture traits in barley (Hordeum vulgare L.). Theor Appl Genet 105:708-715 https://doi.org/10.1007/s00122-002-0992-3
  18. Mano Y, Takahashi H, Sato K, Takeda K (1996) mapping genes for callus growth and shoot regeneration in barley (Hordeum vulgare L.). Breed Sci 46:137-142
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Nishimura A, Ashikari M, Lin SY, Takashi T, Angeles E (2005) Isolation of a rice regeneration quantitative trait loci gene and its application to transformation system. Proc Natl Acad Sci USA 102:11940-11942 https://doi.org/10.1073/pnas.0504220102
  21. Ritala A, Aspegren K, Kurten U, Salmenkallio-Marttila M, Mannonen L, Hannus R, Kauppinen V, Teeri TH, Enari TM (1994) Fertile transgenic barley by particle bombardment of immature embryos. Plant Mol Biol 24:317-325 https://doi.org/10.1007/BF00020170
  22. Taguchi-Shiobara F, Lin SY, Tanno K, Komatsuda T, Yano M, Sasaki T, Oka S (1997) Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L. Theor Appl Genet 95:828-833 https://doi.org/10.1007/s001220050632
  23. Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369-1376 https://doi.org/10.1046/j.1365-313X.1997.11061369.x

Cited by

  1. Translational Genomics in Agriculture: Some Examples in Grain Legumes vol.34, pp.1, 2010, https://doi.org/10.1080/07352689.2014.897909