• International journal of advanced smart convergence
• /
• 제10권3호
• /
• pp.214-224
• /
• 2021

We was confirmed that the antler extract increases the expression of marker genes expressed in the process of bone formation, and that the effect on the increase in the expression of the gene is further increased by fermentation of the antler extract. In addition, the mouse model in which menopausal was induced by ovary extraction significantly reduced the movement distance and exercise time of mice compared to the control group. But the decrease was somewhat alleviated by the administration of the antler extract, and completely restored when the fermented antler extract was administered. In the menopause-induced mice, the body weight ratio of heart, liver, and spleen weights increased compared to the control group, but the antler extract and the antler ferment extract restored the body weight ratio of various organ weights to the level of the control group in the menopause-induced mice. Consequently, this has led to mitigating changes in the metabolism affected by menopause.

• 대한한의학회지
• /
• 제28권4호
• /
• pp.136-147
• /
• 2007

Objective : Recently there are a lot of attempts to treat obesity through energy expenditure. Especially UCP-1 and PPAR-${\delta}$ is known to play a key role for energy dissipation through the increasing thermogenesis. Gambi-hwan extract is a traditional medicine made of herbs containing the polyunsaturate fatty acids related to the energy expenditure. It is expected to reduce the weight by means of the expression of UCP-1 and PPAR-${\delta}$. Meterial and Method : We divided 21 rats into 3 groups and assigned 8 rats respectively. The normal group was administered normal diet, the control group was administered high-fat diet, and the G50 group was administered high-fat diet with Gambi-hwan extracts50 mg/kg. And then the weights of body, food intake, the changes of lipids in blood stream, and the expressions of UCP-1 and PPAR-${\delta}$ on adipose tissues were measured respectively. Result : The reduction of body weight and the increasing tendency of expression of UCP-1 and PPAR-${\delta}$ mRNA were shown in G50 group. In the G50 group the Triglyceride level is decreased and the HDL-cholesterol level and the expression of PPAR-${\delta}$ and UCP-1 protein on Visceral adipose tissue were significantly increased. Conclusion : This result indicates that Gambi-hwan Extract upregulate the expression of UCP-1 and PPAR-${\delta}$ in adipose tissue, which may contribute to reducing the weight of adipose tissue.

• 동의생리병리학회지
• /
• 제19권6호
• /
• pp.1585-1593
• /
• 2005

The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-tang on the stress were studied with cDNA microarray analyses, RT-PCR on hypothalamus using an immobilization-stress mice as an animal model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hrs once a day, and this challenge was repeated for seven· consecutive days. In the change of body weight it showed that the Boshimgeonbi-tang is effected recovery on weight loss caused by the immobilization-stress. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with $CyDye^{TM}$ fluorescence dyes and then hybridized to CDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix4000 series scanner and GenePix $Pro^{TM}$ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis-, stress protein, transcriptional factor, and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosysthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 5.5 fold. The 11 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Ogg1 (DNA repair), Aatk (apoptosis), Dffa (apoptosis), Fkbp5 (protein folding) were restored to the normal one by the treatment of Boshimgeonbi-tang.

• 한국의상디자인학회지
• /
• 제14권3호
• /
• pp.37-49
• /
• 2012

The purpose of this study is to provide basic data of dress design by researching three-dimensional decorative techniques representing flowers, a popular motif in dress design. This study analyzed 44 dresses that were presented in Valentino collection. The result of this study is as follows: First, the detailing expression method can show various patterns and colors through embroidery technique, sub-materials, and bonding techniques in embroidery, beads flower, and ribbon flowers. Second, the fabric flower technique expressed flower image in three-dimension in more various images than do either the ribbon or beads flower technique. Fabric flowers are mostly made from the same material as dresses. The expression technique of fabric flower is classified into the following three classifications: 1) A technique underlying a flower image with one, two and three large flowers, 2) a technique decorating a large area with a number of an identical motif, and 3) a technique highlighting a part of the body or design line by attracting eyes to one point, creating a corsage decorated where a designer wants to emphasize. Third, a silhouette technique realizes a flower image with a silhouette of a dress like sculpted structure and it is thus used less than a detailing expression technique. A flower image is expressed by making use of body lines or emphasizing the face. Finally, flower images on a dress were realized as a full blossomed flower, through visually streamlined shapes and curved lines.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2012년도 춘계 종합학술대회 논문집
• /
• pp.239-240
• /
• 2012

본 연구는 동작놀이를 통한 정신지체 아동들의 신체움직임 변화에 미치는 효과를 보기 위함이다. 특수학교 정신지체 아동 6명을 대상으로 동작놀이 프로그램을 한 달간 주1회 1시간씩 5회기를 실시하였다. 연구결과 장애의 정도에 따라 학습능력, 학습태도에서 차이는 있었지만 신체영역 중 놀이, 행동, 운동성의 능력이 발달되었으며 사회영역 중 자기감정에 따른 표현이나 자신의 긍정적인 모습과 표현활동 능력이 향상되었음을 알 수 있었다.

• Journal of Microbiology and Biotechnology
• /
• 제13권3호
• /
• pp.400-405
• /
• 2003

A gene (hpkA), encoding a histidine protein kinase homolog, has been identified in the upstream region of the espAB operon in Myxococcus xanthus. It encodes a 333 amino acid (35,952 Da) protein with a histidine protein kinase domain in the region from amino acid 90 to 317. Null mutations in the hpkA gene caused formation of loose irregular fruiting bodies, while wild-type strains developed tight hemispherical fruiting bodies under developmental conditions. Sporulation of the hpkA mutant was delayed by at least 12 h compared to that of the wild-type. It appeared that the hpkA mutation increased the expression of the espAB operon by more than 2-fold compared with the wild-type under developmental conditions. Expression of the hpkA gene was low under vegetative conditions, but was highly induced under developmental conditions.

• 복식
• /
• 제25권
• /
• pp.215-231
• /
• 1995

Semiotics is an external expression for show-ing internal meaning, and the signs express the internal meaning. The architects and the designers are very similar each other in using semiotics for expressing their desires. Histori-cally, the design similarity exists between the architecture and the fashion. In this study, the fashion designs are interpreted by using semiotics of architectural design, especially emphasizing external expression methods for representing internal desires. To express the human body's character-istics, the volume, weight support, structural simplicity, controllability and beauty of fashion were used for the fashion designs, and those characters could be found at the architec-tural patterns. Balenciaga's fashion emphasized the volume, the weigh support, the structural simplicity and the controllability, while Roberto Capucci rejected the approach of using women's body curves, but used the volume and simplicity of natures in addition to Balenciaga's heavy weight design patterns. The control and the modern dedication were used to make the simple patterns by Courreges, and then brought the Modernism to the fashion. Courreges used the cutting characters to func-tional design for expressing the structural sim-plicity.

• BMB Reports
• /
• 제45권5호
• /
• pp.293-298
• /
• 2012

The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, $Accp38b$, was isolated from $Apis$ $cerana$ $cerana$ and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that $Accp38b$ was induced by multiple stressors. Notably, the expression of $Accp38b$ was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of $A.$ $cerana$ $cerana$. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.

• Clinical and Experimental Reproductive Medicine
• /
• 제33권1호
• /
• pp.35-35
• /
• 2006

목 적: 본 연구의 목적은 난소 내 인히빈-${\alpha}$에 단백질 발현과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$ 유전자의 발현에 감마선이 미치는 효과를 연구하는 것이다. 연구방법: 미성숙 생쥐에 감마선을 치사량의 25% 효과로 전신 조사하였다. 감마선 조사 후, 0, 3, 6, 12, 24시간이 지나서 난소를 적출하였다. 적출한 난소를 이용하여, 인히빈 ${\alpha}$에 대한 면역조직화학 염색과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$에 대한 RT-PCR을 수행하였다. 결 과: 인히빈 ${\alpha}$ 면역양성반응성은 12시간 방사선 조사 후까지 유지가 되었고, 이후에 감소하였다. 인히빈 ${\alpha}$ mRNA의 발현은 방사선 처리 후에 유의하게 증가하였다. 그러나, 인히빈 ${\beta}A$와 ${\beta}B$의 mRNA의 발현은 유의한 변화가 나타나지 않았다. 결 론: 인히빈은 감마선 조사로 유도된 생쥐난포 폐쇄에 조절적 요소로 작용하는 것으로 사료된다.

• Animal cells and systems
• /
• 제15권1호
• /
• pp.29-36
• /
• 2011

Insect lysozymes are basic, cationic proteins synthesized in fat body and hemocytes in response to bacterial infections and depolymerize the bacterial cell wall. The c-type lysozyme of the insect Spodoptera litura (SLLyz) is a single polypeptide chain of 121 residues with four disulfide bridges and 17 rare codons and is approximately 15 kDa. The full-length SLLyz cDNA is 1039 bp long with a poly(A) tail, and contains an open reading frame of 426 bp long (including the termination codon), flanked by a 54 bp long 5' UTR and a 559 bp long 3' UTR. As a host for the production of high-level recombinant proteins, E. coli is used most commonly because of its low cost and short generation time. However, the soluble expression of heterologous proteins in E. coli is not trivial, especially for disulfide-bonded proteins. In order to prevent inclusion body formation, GST was selected as a fusion partner to enhance the solubility of recombinant protein, and fused to the amplified products encoding mature SLLyz. The expression vector pGEX-4T-1/rSLLyz was then transformed into E. coli BL21(DE3)pLysS for soluble expression of rSLLyz, and the soluble fusion protein was purified successfully. Inhibition zone assay demonstrated that rSLLyz showed antibacterial activity against B. megaterium. These results demonstrate that the GST fusion expression system in E. coli described in this study is efficient and inexpensive in producing a disulfide-bonded rSLLyz in soluble, active form, and suggest that the insect lysozyme is an interesting system for future structural and functional studies.