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Expression of Inhibin in the Whole-body γ-irradiated Mouse Ovary  

Kim, Sang Soo (The Research Institute for Natural Sciences, Hanyang University)
Lee, Chang Joo (The Research Institute for Natural Sciences, Hanyang University)
Yoon, Hyun-Tae (Laboratory of Toxicogemonics, Department of Life Science, Hanyang University)
Yoon, Yong-Dal (Laboratory of Toxicogemonics, Department of Life Science, Hanyang University)
Publication Information
Clinical and Experimental Reproductive Medicine / v.33, no.1, 2006 , pp. 35-35 More about this Journal
Abstract
Objective: The purposes of the present study were to investigate the effect of ${\gamma}$-radiation on the expression of inhibin-${\alpha}$ proteins and genes for inhibin ${\alpha}$, ${\beta}A$, and ${\beta}B$ in the ovary. Methods: Immature mice were whole-body ${\gamma}$-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation, the ovaries were collected and used for immunohistochemistry for inhibin-${\alpha}$, and RT_PCR for inhibin-${\alpha}$, ${\beta}A$, and ${\beta}B$. Results: The expression of the immunoreactive inhibins-${\alpha}$ was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-${\alpha}$ mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of ${\beta}A$ and ${\beta}B$ mRNAs. Conclusion: It might be thought that inhibin acts as one of the regulatory factors in the ${\gamma}$-radiation-induced follicular atresia in mice
Keywords
Mouse ovary; Follicle; Inhibin; Radiation;
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