Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Expression of Inhibin in the Whole-body γ-irradiated Mouse Ovary

감마선이 조사된 미성숙 생쥐 난소 내 인히빈의 발현 변화

  • Kim, Sang Soo (The Research Institute for Natural Sciences, Hanyang University) ;
  • Lee, Chang Joo (The Research Institute for Natural Sciences, Hanyang University) ;
  • Yoon, Hyun-Tae (Laboratory of Toxicogemonics, Department of Life Science, Hanyang University) ;
  • Yoon, Yong-Dal (Laboratory of Toxicogemonics, Department of Life Science, Hanyang University)
  • 김상수 (한양대학교 자연과학연구소) ;
  • 이창주 (한양대학교 자연과학연구소) ;
  • 윤현태 (한양대학교 자연과학대학 생명과학과) ;
  • 윤용달 (한양대학교 자연과학대학 생명과학과)
  • Published : 2006.03.31
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Abstract

Objective: The purposes of the present study were to investigate the effect of ${\gamma}$-radiation on the expression of inhibin-${\alpha}$ proteins and genes for inhibin ${\alpha}$, ${\beta}A$, and ${\beta}B$ in the ovary. Methods: Immature mice were whole-body ${\gamma}$-irradiated with 25% of a lethal dose. At time 0, 3, 6, 12, and 24 hours after the irradiation, the ovaries were collected and used for immunohistochemistry for inhibin-${\alpha}$, and RT_PCR for inhibin-${\alpha}$, ${\beta}A$, and ${\beta}B$. Results: The expression of the immunoreactive inhibins-${\alpha}$ was maintained at 12 hours post-irradiation and reduced thereafter. The expression of inhibin-${\alpha}$ mRNA was significantly increased with the time after the irradiation. However there were no significant changes in the expression of ${\beta}A$ and ${\beta}B$ mRNAs. Conclusion: It might be thought that inhibin acts as one of the regulatory factors in the ${\gamma}$-radiation-induced follicular atresia in mice

목 적: 본 연구의 목적은 난소 내 인히빈-${\alpha}$에 단백질 발현과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$ 유전자의 발현에 감마선이 미치는 효과를 연구하는 것이다. 연구방법: 미성숙 생쥐에 감마선을 치사량의 25% 효과로 전신 조사하였다. 감마선 조사 후, 0, 3, 6, 12, 24시간이 지나서 난소를 적출하였다. 적출한 난소를 이용하여, 인히빈 ${\alpha}$에 대한 면역조직화학 염색과 인히빈 ${\alpha}$, ${\beta}A$, 그리고 ${\beta}B$에 대한 RT-PCR을 수행하였다. 결 과: 인히빈 ${\alpha}$ 면역양성반응성은 12시간 방사선 조사 후까지 유지가 되었고, 이후에 감소하였다. 인히빈 ${\alpha}$ mRNA의 발현은 방사선 처리 후에 유의하게 증가하였다. 그러나, 인히빈 ${\beta}A$와 ${\beta}B$의 mRNA의 발현은 유의한 변화가 나타나지 않았다. 결 론: 인히빈은 감마선 조사로 유도된 생쥐난포 폐쇄에 조절적 요소로 작용하는 것으로 사료된다.

Keywords

Acknowledgement

Supported by : 한국과학재단

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