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HpkA, a Histidine Protein Kinase Homolog, is Required for Fruiting Body Development in Myxococcus xanthus  

Park, Sooyeon (Section of Life Science, Hoseo University)
Kim, Jihoon (Section of Life Science, Hoseo University)
Lee, Bongsoo (Section of Life Science, Hoseo University)
Zusman, David R (Section of Life Science, Hoseo University)
Cho, Kyungyun (Section of Life Science, Hoseo University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.3, 2003 , pp. 400-405 More about this Journal
Abstract
A gene (hpkA), encoding a histidine protein kinase homolog, has been identified in the upstream region of the espAB operon in Myxococcus xanthus. It encodes a 333 amino acid (35,952 Da) protein with a histidine protein kinase domain in the region from amino acid 90 to 317. Null mutations in the hpkA gene caused formation of loose irregular fruiting bodies, while wild-type strains developed tight hemispherical fruiting bodies under developmental conditions. Sporulation of the hpkA mutant was delayed by at least 12 h compared to that of the wild-type. It appeared that the hpkA mutation increased the expression of the espAB operon by more than 2-fold compared with the wild-type under developmental conditions. Expression of the hpkA gene was low under vegetative conditions, but was highly induced under developmental conditions.
Keywords
Myxobacteria; Myxococcus xanthus; hpkA; espAB; fruiting body; sporulation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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