• Bulletin of the Korean Chemical Society
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• v.12 no.1
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• pp.47-51
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• 1991

Spin-lattice relaxation of methyl protons in 2,6-dichlorotoluene and N-methyl phthalimide, each dissolved in CDCl$_3$, has been studied at 34$^{\circ}$C and the contribution from spin-rotation interaction to the relaxation process has been separated from that due to dipole-dipole interactions among methyl protons. The results show that the spin-rotational contributions to the initial rate of relaxation in 2,6-dichlorotoluene and N-methyl phthalimide amount to 18 and 31%, respectively, of the total relaxation rate at 34$^{\circ}$C. The method of separating the spin-rotational contribution from that of dipolar interactions adopted in this paper is based on the well known fact that in an A$_3$ spin system such as methyl protons in liquid phase dipolar relaxation mechanism gives non-exponential decay of the z-component of total magnetization of protons while the random field fluctuation such as spin-rotational mechanism causes exponential decay.

• Applied Biological Chemistry
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• v.43 no.4
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• pp.231-235
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• 2000

The changes of calmodulin levels, calmodulin-binding proteins, and $Ca^{2+}$/calmodulin-dependent glutamate decarboxylase during the growth of tobacco suspension cells were investigated. Tobacco cells exhibited a typical growth curve, including an exponential growth phase between 3 and 5 days after inoculation, and an apparent stationary phase occurring after 5 day. Although slight changes were observed from sample to sample, calmodulin protein levels remained similar during the phases of culture growth. Several $Ca^{2+}-dependent$ calmodulin-binding proteins including 56, 46, 36, and 32-kDa proteins were detected in tobacco cell extracts. The 56-kDa protein was identified as glutamate decarboxylase by Western-blot analysis using an anti-GAD monoclonal antibody. The levels of GAD protein and the specific activity of GAD enzyme were highest during the middle exponential phase of the culture growth cycle. These data suggest that $Ca^{2+}$/calmodulin-dependent glutamate decarboxylase is modulated during the growth of tobacco suspension cells.

• Journal of KIISE:Databases
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• v.29 no.3
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• pp.217-229
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• 2002

Regular path expressions are primary elements for formulating queries over the semistructured data that does not assume the conventional schemas. In addition, the query pruning is an important optimization technique to avoid useless traversals in evaluating regular path expressions. However, the existing query pruning often fails to fully optimize multiple regular path expressions, and the previous methods that post-process the result of the existing query pruning must check exponential combinations of sub-results. In this paper, we present a new query pruning technique that consists of the preprocessing phase and the pruning phase. Our two-phase query pruning is affective in optimizing multiple regular path expressions, and is more scalable than the previous methods in that it never check the exponential combinations of sub-results.

• Korean Journal of Veterinary Service
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• v.30 no.3
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• pp.291-304
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• 2007

Mutants of an obligate aerobic bacterium, Vitreoscilla, that have deficiency in heat-labile catalase-peroxidase hydroperoxidase I (HPI) were created by EMS treatment. The catalase-peroxidase HPI-deficient mutant showed substantially lower peroxidase activity in exponential and mid-stationary phase compared with the wild type strain. In late stationary phase, the mutant exhibited no peroxidase activity. Peroxidase deficiency in the mutant was revealed by polyacrylamide gels stained for peroxidase activity. Characteristically, catalase levels in the mutant increased about 14- and 8-fold during growth in the exponential and stationary phases, respectively, compared to those in the wild type, suggesting a compensatory effect for protection from $H_2O_2$ toxicity. The mutant showed differences in physiology from the wild type: retardation in growth rate and decrease in oxygen consumption. Both the wild type and the catalase-peroxidase HPI-deficient mutant of Vitreoscilla had lower growth rates in media containing increasing $H_2O_2$ concentrations. However, the mutant exhibited an additionally decreased growth rate after 6 to 8 h of growth compared to the wild type. The wild type was resistent up to 20 mM $H_2O_2$, whereas the mutant was very sensitive to high concentrations of exogenous $H_2O_2$. Although elevated catalase levels would provide protection of the bacteria from the deleterious effect of $H_2O_2$, it did not appear to be complete. Cell-free extracts of the mutant showed decreased NADH oxidation rates and higher accumulation of $H_2O_2$ during this oxidation. These results may account for the impaired growth and earlier onset of death phase by the catalase-peroxidase HPI-deficient mutant of Vitreoscilla.

• Journal of the Institute of Convergence Signal Processing
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• v.9 no.1
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• pp.1-9
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• 2008

One of the problems for implementing the spot detection phase in the 2-DE gel image analysis program is the eliminating noises in the image. Remained noises after the preprocessing phase cause the over-segmented regions by the segmentation phase. To identify and exclude the over-segmented background regions, if we use the fixed thresholding method that is choosing an intensity value for the threshold, the spots that is invisible by the eyes but mean a very small amount proteins which have important role in the biological samples could be eliminated. This paper propose an adaptive thresholding method that come from an idea that is got on statistical analysing for the prominences of the peaks. The adaptive thresholding method works as following. Firstly we calculate an average prominence value curve and fit it to exponential function curve, as a result we get parameters for the exponential function. And then we calculate a threshold value by using the parameters and probability distribution of errors. Lastly we apply the threshold value to the region for determining the region is a noise or not. According to the probability distribution of errors, the reliability is 99.85% and we show the correctness of the proposed method by representing experiment results.

• Molecules and Cells
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• v.22 no.3
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• pp.336-342
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• 2006

Real time reverse transcription (RT)-PCR was used to quantify the expression of the botulinum neurotoxin type A (BoNT/A) gene (cntA) by normalization with the expression of 16S rRNA. The method were confirmed by monitoring the mRNA levels of cntA during growth in five type A strains. In all but one of the strains the expression of cntA mRNA was maximal in the late exponential phase, and approximately 35-fold greater than in the early exponential phase. The concentration of the extracellular BoNT/A complex detected by ELISA was highest in stationary phase. Sodium nitrite and sorbic acid completely inhibited growth at 20 ppm and $4mg\;ml^{-1}$, respectively. CntA expression became lower in proportion to the concentration of sorbic acid, and this reduction was confirmed by mouse bioassay. Our results show that real time RT-PCR can be used to quantify levels of C. botulinum type A neurotoxin transcripts and to assess the effects of food additives on botulinal risk.

• Journal of Environmental Science International
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• v.15 no.12
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• pp.1177-1183
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• 2006

This study was carried out to evaluate effect of sludge characteristics (pH, SVI, anaerobic storage, wet density and growth phase) on the sedimentation of bulking sludge of paper manufacturing plant. The results showed that mix hulking sludge with sewage sludge (10-60%) caused the decrease of SVI, the settled sludge concentration was increased. Increase of anaerobic storage time (0 to 8 day) was increased SVI, sedimentation performance was deteriorated. The sedimentation was improved by addition of loess (0.2-5.0 g/L), because wet density of sludge was increased and SVI was decreased. At an exponential phase, the ratio of sedimented/growthed sludge concentration was decreased, the ratio was increased after stationary phase.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.201-202
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• 2006

To analyze the dynamic characteristics of the bundle drawing process, we employed a Random Phase Spectrum method to generate stochastic test signals that had a given autocorrelation function. And the spectra of the dynamics of the process outputs were obtained, based on the dynamic model of the bundle drawing process. Results showed that the RPS method was very effective to generate stochastic signals that had an exponential function form. The drawing process had the traits that there existed a special frequency range, incurring the process resonance.

• Transactions of the Korean Society of Mechanical Engineers
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• v.16 no.5
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• pp.933-942
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• 1992

The effects of the folwrate on the dropsize from the two-phase coaxial nozzle are investigated by using the direct photographic method and the empirical equation is obtained. For the photography, the light source which the life time is the order of 100ns is fabricated and the lens is the zoom lens which has the MICRO function with a teleconvertor. The distillated water and the compressed air of the surrounding temperature are injected and atomized. For the purpose of the exact adjustment and measurement of the flowrates, the two rotameters are used. As a result of this study, the sauter mean diameter of droplets has a tendency for a logarithmic function with air flowrate and for a exponential function with water flowrate.

• Journal of Power Electronics
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• v.19 no.5
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• pp.1162-1170
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• 2019

Finite-control-set model predictive control (FCS-MPC) is a promising control scheme for two-level voltage-source inverters (TL-VSIs). However, two main issues arise in the classical FCS-MPC method: an exponentially-increasing computational time and a low steady-state performance. To solve these two issues, a novel FCS-MPC method has been proposed for n-phase TL-VSIs in this paper. The basic idea of the proposed method is to carry out the FCS-MPC scheme of TL-VSIs for one leg by one leg, like a "pipeline". Based on this idea, the calculations are reduced from exponential time to linear time and its current waveforms are improved by applying more switching states per sampling period. The cases of three-phase and five-phase TL-VSIs were tested to verify the effectiveness of proposed method.