• Journal of the Korean Wood Science and Technology
• /
• 제47권2호
• /
• pp.178-188
• /
• 2019

Licorice, which has an extensive history of use as an herbal medicine, has been suggested to have oral health benefits. However, to date, no systematic study has been conducted on the preparation method of licorice extracts for oral health. In this study, licorice extracts prepared using water and ethanol were investigated for its ability to inhibit the biofilm formation of Streptococcus mutans. The licorice extract prepared with around 60% ethanol effectively inhibited the biofilm formation of S. mutans. Licorice extracted with 50% ethanol almost completely inhibited the biofilm formation at 1.5 g/L of licorice extract. This inhibitory activity was confirmed in a microplate assay and a flow cell system. Glycyrrhetic acid was extracted from licorice effectively with 60% ethanol concentration. The strong inhibitory activity of glycyrrhetic acid and the synergistic inhibition with glycyrrhizin on biofilm formation were suggested as major reasons for a concentration-specific extraction. These results suggest that licorice extract prepared using around 60% ethanol effectively inhibits the biofilm formation of S. mutans.

• Journal of Ginseng Research
• /
• 제3권1호
• /
• pp.40-53
• /
• 1979

In order to investigate the optimal conditions which affects to extraction of ginseng extract and saponin in ginseng extract, experiment was carried out varing with ethanol percentage, extraction time, temperature, sol$.$vent and Plant Parts. The results art as follows: 1. The amounts of ginseng saponin was increased according to increanation of ethanol Percentage while the amounts of ginseng extract was decreased. 2. The amounts of ginseng extract was increased as the prolongation of extraction time, on the ether hand, ginseng saponin contents increased lentil 40hr. and decreased after that. 3. By the raise of extract temperature, both of the amounts of ginseng saponin and ginseng extract was increased two times and four times. respectively. 4. The total amounts ginseng extract was obtained 22.86u when the water used as the extraction solvent, 11.28% on ethanol and 11.04U on methanol, in the order. and the saponin contents gained when the extraction solvents of water, methanol and ethanol 7.47%, 12.36% and 12.77%, respectively. 5. It showed 9.23% of ginseng extract in epidermis and 8.4% of ginseng saponin in tail Part of raw ginseng and in the case of dried ginseng, ginseng extract and saponin showed the most amounts in epidermis of 18.28% and 19.35%, respectively. 6. The ratio of panaxadiol and panaxatriol contents of ginseng saponin was almost same when it was extracted varing with ethanol percentage and extraction time (duration), and the more alcohol percentage and the longer extraction time increased, the more fractional content of ginseng saponin was extracted.

• 한국식품위생안전성학회지
• /
• 제10권1호
• /
• pp.23-28
• /
• 1995

The effect of the ethanol extract from salviae miltiorrhizae radix (Salvia miltiorrhiza) on the microbial growht and the stability of the extracted antimicrobial material were investigated. The ethanol extract had strong growth inhibition activity (MIC, 3.13~50.0 $\mu\textrm{g}$/ml) against Gram-positive bacteria such as B. subtilis, L. monocytogenes and S. aureus. Among Gram-positive bacteria tested, B. subtilis was the most susceptible to the extracted substance. While the antimicrobial activity of the ethanol extract was weak (MIC, 400~800 $\mu\textrm{g}$/ml) to E. coli and yeasts (C. albicans, Sacch. diastaticus). The ethanol extract had bactericidal action at higher concentration than MIC against B. subtilis, while the extract had only bacteriostatic action against S. aureus. The extracted antimicrobial substance was stable in the pH range of 4.0 to 10.0, heat treatment at 121$^{\circ}C$ for 15 min, and freezing and thawing

• Journal of Ginseng Research
• /
• 제4권1호
• /
• pp.72-87
• /
• 1980

Red ginseng tails were extracted with ethanol solutions over a range of concentrations and temperature conditions. Investigations were carried out to study the effects of treatments on yields, soluble solids, saponin and precipitate occured in red ginseng extract beverage during storage. It was found that: (1) Higher concentration of ethanol at low temperature resulted in less yield of crude extract (2) The amount of precipitate in the non-purified extract beverage were less with decrease in ethanol concentration used (3) The treatment for purification of extracts and storage of purified extract at 37$^{\circ}C$ for 6 months had no effect on HPLC chromatogram pattern of saponins (4) The amount of purified extract decreased by purification treatment and more decrease was found as the temperature and concentration of ethanol increased. For Preparation of red ginseng extract beverage, the treatment of extracts with ethanol at low temperature was found to be more effective to minimize precipitation in tile beverage.

• 생명과학회지
• /
• 제11권4호
• /
• pp.321-327
• /
• 2001

The purpose of this study was to investigate the nitrite scavenging activity of the ethanol and water extracts obtained from Hizikia fusiforme and Sargassum fulvelum using sodium nitrite under different pH conditions. The nitrite scavenging activity was appeared in all the extracts examined and it showed the highest value at pH 1.2 The ethanol extract of Hizikia fusiforme provided higher activity than the water extract of that. Ohterwise, the nitrite scavenging activity of Sargassum fulvelum ethanol extract was similar to that Sargassum fulvelum water extract. There was significant difference among concentration of extracts Hizikia fusiforme and Sargassum fulvelum. Also, nitrite scavenging activity of the extracts Hizikia fusiforme and Sargassum fulvelum decreased with higher pH condition. In addition, the activity was increaed with higher concentration of extracts Hizikia fusiforme and Sargassum fulvelum in the course of reaction time 30 minute to 3 hour.

• Applied Biological Chemistry
• /
• 제48권3호
• /
• pp.273-279
• /
• 2005

To develop cosmetics using Jindalae flowers (Rhododendron mucronulatum), the surface tensions of extracts were measured and the properties and stability of cream with extracts were investigated. The surface tension of 0.1% ethanol extract was 30.42 mN/m and that of distilled water was 72.2 mN/m. The surface tension of cream with 0.1% ethanol extract was similar to that of sample cream and the measured pH were weakly alkalic. The surface tension of 1% ethanol extract was the lowest value of 24.98 mN/m, the measured pH of cream with 1% ethanol extract was weakly acidic and the particle size of cream was stable. According to an oscillatory test, linear viscoelastic region was extended by adding of 1% water extract and 1% ethanol extract to cream, indicating that the cream had greater enhanced resistance for preserving inner structure as compared to outside stress.　Besides, as a result of the diminished loss angle of ethanol extract cream, the elasticity of cream was increased more than that of sample cream and cream with 0.1% ethanol extract. In contrast, in the case of the increased loss angle of water extract cream, the viscosity of cream was increased. In conclusion, Rhododendron mucronulatum can be deliberated as a cosmetic material because 0.1% water and ethanol extracts showed efficacious physiological activities and cream with 1% extracts could extend linear viscoelastic region.

• KSBB Journal
• /
• 제30권4호
• /
• pp.155-160
• /
• 2015

The present study was to investigate the antioxidant activity in ethanol and water extracts of root and stem of Sargassum coreanum. Antioxidant activities were evaluated by total polyphenol contents, DPPH radical scavenging activity, chelating effect, reducing power, and rancimat method. Total polyphenol contents of ethanol and water extracts were 32.79 mg/g and 15.55 mg/g, respectively. Ethanol extract showed higher DPPH radical scavenging activity than water extract and similar activity to BHT. Reducing power of extracts was increased in a concentration-dependent manner and ethanol extract had more reducing power than water extract. Ethanol and water extracts have little chelating effect at all concentrations. Antioxidant index (AI) of ethanol extract measured by Rancimat was higher than that of water extract, but their AI was lower than that of BHT. These results indicate that ethanol extract of S. coreanum root and stem has more potent antioxidant activity than water extract through DPPH radical scavenging and reducing power, and could potentially be used as a good source of natural antioxidants.

• Food Science and Biotechnology
• /
• 제15권4호
• /
• pp.599-602
• /
• 2006

The effects of ethanol extract from radish seeds on gastrointestinal function in rats were investigated. Rats were divided into 2 groups of 8 rats each, the basal group and the radish seed ethanol extract group. The radish seed ethanol extract group had a significantly decreased gastrointestinal transit time, but a significantly increased fecal weight compared with the basal group. The radish seed ethanol extract group also had significantly higher duodenal villus height and greater colonic mucosal thickness than the basal group. The radish seed ethanol extract group had a greater proliferation of 5-bromo-2-deoxy-uridine (BrdU) immunoreactive cells in the gastric mucosa as well as in the mucosa and submucosa of the small and large intestine than did the basal group. Thus, radish seed ethanol extract may be useful in preventing constipation based on the observation of an increase in fecal weight, a decrease in gastrointestinal transit time, and positive changes in the intestinal mucosa.

• Environmental Analysis Health and Toxicology
• /
• 제3권3_4호
• /
• pp.29-37
• /
• 1988

Experiments were performed on mice to investigate the effect of panax ginseng extracts on the immunotoxicity of ethanol. Immune response were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette froming cell (RFC) and macrophage activity in mice, sensitized and challenged with sheep red blood cells. The weight of liver, spleen and thymus were measured. Following results obtained in this experiment. The exposure of ethanol decreased humoral and cellular immune response, the body weight and macrophage activity. Ginseng extracts such as ethanol extract, petroleum ether extract and n-butanol fraction were significantly increased the body weight. The administration of ginseng ethanol extract and ginseng petroleum ether extract were restored or increased humoral and cellular immune response. Macrophage activity was decreased by ethanol, but restored by the ginseng extracts.

• 한국식품위생안전성학회지
• /
• 제36권1호
• /
• pp.86-92
• /
• 2021

식용곤충인 갈색거저리 유충을 기능성 소재로 활용하기 위하여 갈색거저리 유충 추출물의 산화방지 성분 및 활성을 확인하였다. 동결건조한 갈색거저리 유충 분말의 일반 성분은 수분함량 0.80 ± 0.06%, 조회분 3.29 ± 0.03%, 조단백질 54.07 ± 0.24%, 조지방 36.78 ± 0.66%이었다. 갈색거저리 유충 분말의 ethanol 농도별(95%, 70%, 50%, 물) 추출물의 총 phenolic compounds 함량은 50% ethanol 추출물에서 459.23 ± 1.05 mg/100 g으로 가장 높았으며, 총 flavonoid compounds 함량은 물 추출물에서 19.86 ± 0.69 mg/100 g으로 가장 높았다. 갈색거저리 유충 분말 추출물의 DPPH radical 소거 활성은 70% ethanol 추출물에서 82.60 ± 0.00%로 가장 높았으며, 물, 50% ethanol, 95% ethanol 추출물 순으로 높았다. ABTS radical 소거 활성은 50% ethanol 및 물 추출물에서 각각 97.57 ± 0.16%와 95.33 ± 0.41%로 높게 나타났으며, 유의적 차이는 없었다. 환원력의 경우 물 추출물에서 유의적으로 가장 높았으며, 50% ethanol, 70% ethanol, 95% ethanol 추출물 순이었다. 본 연구결과 갈색거저리 유충의 산화방지 성분의 함량이 많고 산화방지 활성을 우수함을 확인하였으며, 기능성 소재로서 활용할 가치가 있는 것으로 나타났다.