• Korean Journal of Optics and Photonics
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• v.29 no.3
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• pp.99-103
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• 2018

Optical coherence elastography (OCE) is based on optical coherence tomography (OCT), which is a noninvasive, high-resolution, cross-sectional imaging technique. In this paper, we have developed dynamic optical coherence elastography to measure elasticity, a mechanical property of tissue, by phase difference. A piezoelectric actuator was used for sinusoidal mechanical loading of samples. Before applying this method to biomaterial, we assessed the feasibility of OCE with samples of sponge, eraser, and sharp lead. Cross-sectional and phase-difference images of the sample were obtained under sinusoidal loading. The strain rate was calculated from the phase-difference information. To obtain the envelope of the phase-difference oscillations along the horizontal direction, Hilbert transformation was performed at each depth. The elevation of the envelope was represented by color mapping, and we could measure the relative elasticity within the sample by comparing the elevations. Finally, there was an advantage when we calculated the shear rate using self-interference in the sample arm, instead of the interference between sample and reference arms.

• Transactions of Materials Processing
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• v.25 no.2
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• pp.91-95
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• 2016

This paper is concerned with modeling of a ductile fracture criterion for sheet metal considering anisotropy to predict the sudden fracture of advanced high strength steel (AHSS) sheets during complicated forming processes. The Lou−Huh ductile fracture criterion is modified using the Hill’s 48 anisotropic plastic potential instead of the von Mises isotropic plastic potential to take account of the influence of anisotropy on the equivalent plastic strain at the onset of fracture. To determine the coefficients of the model proposed, a two dimensional digital image correlation (2D-DIC) method is utilized to measure the strain histories on the surface of three different types of specimens during deformation. For the derivation of an anisotropic ductile fracture model, principal stresses (𝜎₁,𝜎₂, 𝜎₃) are expressed in terms of the stress triaxiality, the Lode parameter, and the equivalent stress (𝜂_𝐻, 𝐿,) based on the Hill’s 48 anisotropic plastic potential. The proposed anisotropic ductile fracture criterion was quantitatively evaluated according to various directions of the maximum principal stress. Fracture forming limit diagrams were also constructed to evaluate the forming limit in sheet metal forming of AHSS sheets over a wide range of loading conditions.

• Archives of Pharmacal Research
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• v.18 no.3
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• pp.173-178
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• 1995

Ciprofloxacin resistance mechanisms were studied by investigating the inhibitory effect of ciprofloxacin on the gyrase-mediated DNA supercoiling and the intracellular accumulation of ciprofloxacin in clinical isolates of Pseudomonas aeruginosa. A higher amount of ciprofloxacin was required to inhibit the gyrases purified from the ciprofloxacin-resistant strains than that from the sensitive strain. Reconstitution of heterologous gyrase subunits from different strains revealed alterations in the A and/or the B subunits of gyrase in these strains. In addition, the resistant strains accumulated approximately a half amount of ciprofloxacin inside the cells, compared to the sensitive strain. However, when the active efflux was blocked by carbonyl cyanide m-chlorophenyl hydrazone treatment, intracellular concentration of ciprofloxacin was elevated about 4-7 fold in these strains, while the sensitive strain was not significantly affected by this treatment, indicating that the ciprofloxacin-resistant strains developed a drug efflux system. Interestingly, these resistant strains expressed an envelope protein of approximately 51 kD. These studies suggest that alterations in the gyrase as well as the active drug-efflux system conferred dual ciprofloxacin resistance mechanisms to these clinical isolates of P. aeruginosa.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.410-416
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• 2022

Erwinia amylovora, the causal agent of fire-blight disease in apple and pear trees, was first isolated in South Korea in 2015. Although numerous studies, including omics analyses, have been conducted on other strains of E. amylovora, studies on South Korean isolates remain limited. In this study, we conducted a comparative proteomic analysis of the strain TS3128, cultured in three media representing different growth conditions. Proteins related to virulence, type III secretion system, and amylovoran production, were more abundant under minimal conditions than in rich conditions. Additionally, various proteins associated with energy production, carbohydrate metabolism, cell wall/membrane/envelope biogenesis, and ion uptake were identified under minimal conditions. The strain TS3128 expresses these proteins to survive in harsh environments. These findings contribute to understanding the cellular mechanisms driving its adaptations to different environmental conditions and provide proteome profiles as reference for future studies on the virulence and adaptation mechanisms of South Korean strains.

• The Journal of Korean Physical Therapy
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• v.9 no.1
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• pp.59-70
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• 1997

The ability of neurotropic alpha herpesviruses to replicate within synaptically linked neurons has made these pathogens valuable tools for transneuronal analysis. Recent studies suggest that unique gene products expressed by genetically engineered strains of virus may permit the use of multiple strains in complex tracing paradigms. In the present study we have examined the invasiveness of two genetically engineered strains of the swine pathogen known as pseudorabies virus(PRV). The two strains were isogenic with the attenuated Bartha strain of PRV; in one strain a lacZ reporter gene was inserted into the gC locus (PRV-BaBlu; $4.75\times10^8pfu/ml$) contrained a PRV envelope glycoprotein gene that was absent in PRV-BaBlu. Simultaneous or temporally separated sequential injection of $4\mu\ell$ of each strain into the ventral wall of the stomach produced a predictale course of retrograde synaptic infection. The results were as follows: 1. PRV-BaBlu and PRV-D infected the dorsal motor nucleus of vagus nerve(DMV) and paraventricular nucleus(PVN). 2. Invasion and replication of PRV-D occured at a faster rate than the parental strain or PRV-BaBlu. 3. PRV-D was much more virulent than PRV-BaBlu or the parental strain. 4. Co-injection of PRV-D and PRV-BaBlu produced an infection that was more virulent than that produced by the parental strain (PRV-Bartha), 5. Neurons in DMV were permissive to co-infection with PRV-D and PRV-BaBlu when they were injected simultaneously into the same site. 6. Replication of PRV-BaBlu was compromised by prior infection of the same circuit with PRV-D. 7. Prior infection of neurons with PRV-D maked them resistant to infection with PRV-BaBlu.

• Structural Engineering and Mechanics
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• v.18 no.5
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• pp.627-644
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• 2004

In this study, the endochronic theory was used to investigate the collapse of thick-walled tubes subjected to external pressure and axial tension. The experimental and theoretical findings of Madhavan et al. (1993) for thick-walled tubes of 304 stainless steel subjected to external pressure and axial tension were compared with the endochronic simulation. Collapse envelopes for various diameter-to-thickness tubes under two different pressure-tension loadings were involved. It has been shown that the experimental results were aptly described by the endochronic approach demonstrated from comparison with the theoretical prediction employed by Madhavan et al. (1993). Furthermore, by using the rate-sensitivity function of the intrinsic time measure proposed by Pan and Chern (1997) in the endochronic theory, our theoretical analysis was extended to investigate the viscoplastic collapse of thick-walled tubes subjected to external pressure and axial tension. It was found that the pressure-tension collapse envelopes are strongly influenced by the strain-rate during axial tension. Due to the hardening of the metal tube of 304 stainless steel under a faster strain-rate during uniaxial tension, the size of the tension-collapse envelope increases.

• Journal of the Korea institute for structural maintenance and inspection
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• v.6 no.1
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• pp.223-231
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• 2002

The pilot tests for the development of biaxial failure envelope of high strength concrete of reactor containments were performed. To apply biaxial loads to concrete, the plate specimens were used. The technical difficulties encountered on the development of a suitable biaxial test setup were discussed. To decide the optimum thickness of plate specimen, the nonlinear finite element analyses using ABAQUS were performed for a 1/8 model of cylindrical specimen(${\Phi}150{\times}300$) and four 1/4 models of plate Specimens ($200{\times}200{\times}T$(=30, 50, 60, 70)mm) under uniaxial compression. Analytical values and test data of relative strength ratio between those specimens with different geometric shapes were also compared. The various test data were obtained under uniaxial compression, uniaxial tension, and biaxial compression and then the stress-strain responses were plotted. The test data indicated that the strength of concrete under biaxial compression, $f_1/f_2=-1/-1$, is 15 percent larger than that under uniaxial compression and the poisson's ratio of concrete is 0.16. Teflon pads employed to eliminate friction between test specimen and loading platens showed an excellent effect under biaxial compression.

• Structural Engineering and Mechanics
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• v.14 no.4
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• pp.435-454
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• 2002

The paper proposes analytical forms able to represent with very good approximation the constitutive law experimentally deducible by means of uniaxial cyclic compressive tests on material having softening post-peak behaviour in compression and negligible tensile strength. The envelope, unloading and reloading curves characterizing the proposed model adequately approach structural responses corresponding to different levels of nonlinearity and ductility, requiring a not very high number of parameters to be calibrated experimentally. The reliability of the model is shown by comparing the results that it is able to provide with the ones analytically deduced from two reference models (one for concrete, another for masonry) available in the literature, and with experimental results obtained by the authors in the framework of a research in progress.

• Structural Engineering and Mechanics
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• v.62 no.1
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• pp.43-54
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• 2017

In this study, the failure behavior of composite material in the biaxial and off-axis loading is studied based on a computational micromechanical model. The model is developed so that the combination of mechanical and thermal loading conditions can be considered in the analysis. The modified generalized plane strain assumption of the theory of elasticity is used for formulation of the micromechanical modeling of the problem. A truly meshless method is employed to solve the governing equation and predict the distribution of micro-stresses in the selected RVE of composite. The fiber matrix interface is assumed to be perfect until the interface failure occurs. The biaxial and off-axis loading of the SiC/Ti and Kevlar/Epoxy composite is studied. The failure envelopes of SiC/Ti and Kevlar/Epoxy composite in off-axis loading, biaxial transverse-transverse and axial-transverse loading are predicted based on the micromechanical approach. Various failure criteria are considered for fiber, matrix and fiber-matrix interface. Comparison of results with the available results in the litreture shows excellent agreement with experimental studies.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.04a
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• pp.565-570
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• 2000

The objective of this paper is to analyse the high-strength concrete columns subjected to reversed cyclic and axial loads by using nonlinear analysis model and compare the experimental results with analysis. The analytical parameters are the compressive strength of concrete, spacing of lateral reinforcement and lateral reinforcement ratio. In this study, the proposed analytical model takes ito account the influence of confined concrete, tension stiffening and strain hardening of steel. The high-strength concrete columns are used to model fiber section element. The analysis results are shown comparatively good prediction on envelope curve, accumulative dissipated energy, deformability and so on.