The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Comparing Protein Expression in Erwinia amylovora Strain TS3128 Cultured under Three Sets of Environmental Conditions

  • Lee, Jongchan (Department of Plant Science and Technology, Chung-Ang University) ;
  • Choi, Junhyeok (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Jeongwook (Department of Plant Science and Technology, Chung-Ang University) ;
  • Cho, Yongmin (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kang, In-Jeong (Division of Crop Cultivation and Environment Research, National Institute of Crop Science) ;
  • Han, Sang-Wook (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2022.05.20
  • Accepted : 2022.06.21
  • Published : 2022.08.01
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Abstract

Erwinia amylovora, the causal agent of fire-blight disease in apple and pear trees, was first isolated in South Korea in 2015. Although numerous studies, including omics analyses, have been conducted on other strains of E. amylovora, studies on South Korean isolates remain limited. In this study, we conducted a comparative proteomic analysis of the strain TS3128, cultured in three media representing different growth conditions. Proteins related to virulence, type III secretion system, and amylovoran production, were more abundant under minimal conditions than in rich conditions. Additionally, various proteins associated with energy production, carbohydrate metabolism, cell wall/membrane/envelope biogenesis, and ion uptake were identified under minimal conditions. The strain TS3128 expresses these proteins to survive in harsh environments. These findings contribute to understanding the cellular mechanisms driving its adaptations to different environmental conditions and provide proteome profiles as reference for future studies on the virulence and adaptation mechanisms of South Korean strains.

Keywords

Acknowledgement

This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Grant No. PJ014934), Rural Development Administration, Republic of Korea. This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2022 (awarded to Junhyeok Choi).

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