• 가족자원경영과 정책
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• 제21권2호
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• pp.73-93
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• 2017

The purpose of this study was to investigate the influences of work-family conciliation strategies on work-family conflict and enrichment in dual-earner couples who live in Daegu with children. The subjects of this study were 176 dual-earner couples, who have under 16 youngest children and work over 15 hours each week. The research tool was questionnaires. For data analysis, factor analysis, Cronbach ${\alpha}$, paired t-test and multiple regression were performed. The main results of this study were as following. First, there were significant differences in management and planning skill strategy, work-family conflict and enrichment according to gender. Second, positive attitudes strategy toward multiple responsibilities, wives use, had an influence on work${\rightarrow}$family conflict in wives. Also, partner coping, management and planning skill, and positive attitudes strategy, wives use, and management and planning skill strategy, husbands use, had an influence on family${\rightarrow}$work conflict in wives. And management and planning skill and professional adjustment strategy, husbands use, had an influence on family${\rightarrow}$work conflict in husbands. work-family conciliation strategies had no influence on work${\rightarrow}$family conflict. Third, positive attitudes strategy toward multiple responsibilities, wives use, had an influence on work${\rightarrow}$family enrichment in wives. Also, partner coping strategy, wives use, and management and planning skill strategy, husbands use, had an influence on family${\rightarrow}$work enrichment in wives. And positive attitudes strategy toward multiple responsibilities, husbands use, had an influence on work${\rightarrow}$family enrichment in husbands. Also management and planning skill strategy, wives use, and partner coping, professional adjustment and management and planning skill strategy had an influence on family${\rightarrow}$work enrichment in husbands.

• Mass Spectrometry Letters
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• 제6권2호
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• pp.31-37
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• 2015

Phosphorylation upon protein is well known to a key regulator that implicates in modulating many cellular processes like growth, migration, and differentiation. Up to date, grafting of multidimensional separation techniques onto advanced mass spectrometry (MS) has emerged as a promising tool for figuring out the biological functions of phosphorylation in a cell. However, advanced MS-based phosphoproteomics is still challenging, due to its intrinsic issues, i.e., low stoichiometry, less susceptibility in positive ion mode, and low abundance in biological sample. To overcome these bottlenecks, diverse techniques (e.g., SCX, HILIC, ERLIC, IMAC, TiO₂, etc.) are continuously developed for on-/off-line enrichment of phosphorylated protein (or peptide) from biological samples, thereby helping qualitative/quantitative determination of phosphorylated protein and its phosphorylated sites. In this review, we introduce to the overall views of enrichment tools that are universally used to selectively isolate targeted phosphorylated protein (or peptide) from ordinary ones before MS-based phospoproteomic analysis.

• Mass Spectrometry Letters
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• 제15권1호
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• pp.1-25
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• 2024

Protein glycosylation, a highly significant and ubiquitous post-translational modification (PTM) in eukaryotic cells, has attracted considerable research interest due to its pivotal role in a wide array of essential biological processes. Conducting a comprehensive analysis of glycoproteins is imperative for understanding glycoprotein bio-functions and identifying glycosylated biomarkers. However, the complexity and heterogeneity of glycan structures, coupled with the low abundance and poor ionization efficiencies of glycopeptides have all contributed to making the analysis and subsequent identification of glycans and glycopeptides much more challenging than any other biopolymers. Nevertheless, the significant advancements in enrichment techniques, chromatographic separation, and mass spectrometric methodologies represent promising avenues for mitigating these challenges. Numerous substrates and multifunctional materials are being designed for glycopeptide enrichment, proving valuable in glycomics and glycoproteomics. Mass spectrometry (MS) is pivotal for probing protein glycosylation, offering sensitivity and structural insight into glycopeptides and glycans. Additionally, enhanced MS-based glycopeptide characterization employs various separation techniques like liquid chromatography, capillary electrophoresis, and ion mobility. In this review, we highlight recent advances in enrichment methods and MS-based separation techniques for analyzing different types of protein glycosylation. This review also discusses various approaches employed for glycan release that facilitate the investigation of the glycosylation sites of the identified glycoproteins. Furthermore, numerous bioinformatics tools aiding in accurately characterizing glycan and glycopeptides are covered.

• 한국콘텐츠학회논문지
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• 제15권11호
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• pp.202-214
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• 2015

본 연구는 다문화가정 부부의 특별한 개별적 요구에 기반을 둔 부부 의사소통 및 관계증진 프로그램을 개발하고 적용 효과를 살피는 데 그 목적을 두었다. 본 프로그램은 기존 프로그램 및 선행연구 검토와 참여자의 요구조사, 전문가 면담의 과정을 통해 개발되었고, 4쌍의 다문화가정 부부에게 11주간(주 1회, 회당 120분) 부부 대화기법, 부부 상담을 활용한 부부의사소통 중재 프로그램으로 적용되었다. 프로그램이 실행되는 동안 수집된 녹음파일, 활동지, 연구자의 현장노트 등의 질적자료를 통해 살펴본 프로그램의 효과는 다음과 같다. (1) 다문화가정 부부의 친밀성이 향상되었고, (2) 부부간의 대화가 증가되었으며, (3) 특히 부부간의 의사소통 방식은 "역기능적 의사소통"에서 "협력적인 의사소통"으로 변화되었다. 결론적으로 이러한 변화가 부부관계 증진에 긍정적인 영향을 미치는 것으로 나타났다. 본 연구에서 개발한 부부 의사소통 프로그램은 다문화가정 부부대화의 물꼬를 열었다는 점과 부부갈등을 해결하고 관리하기 위해 의사소통 전략과 전문적인 상담지원을 하였다는 점에서 의의를 가진다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.199-200
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• 2003

After more than 5 years of turbot culture in China, low percent survival and high occurrences of abnormally pigmented juveniles are still major problems for fish farmer. Much research has been directed toward determining the optimal feeding strategies and nutritional requirements for marine flatfish larvae, and considerable advances have been made. The most common live feeds include :Artemia, rotifer and copepods. (omitted)

• 영재교육연구
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• 제13권4호
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• pp.45-63
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• 2003

본 연구의 목적은 과학영재학교의 교육과정 운영실태와 이와 관련된 학생들의 인식을 분석하는데 있다. 이를 위하여 과학영재학교 교육과정 문서 및 학사 운영과정의 통계 자료를 통해 실태를 밝히고, 과학영재학교 학생을 대상으로 교육과정 편제 및 운영, 교수·학습 방법, 평가 방법에 관하여 설문을 실시하여 그 결과를 분석하였다. 과학영재학교 교육과정 편제 및 운영에 대한 학생들의 인식을 조사한 결과, 심화선택과목의 학점 비중을 더 높여야한다는 의견과 보통교과의 학점을 줄이고 전공교과의 학점을 늘려야 한다는 의견이 상대적으로 높게 나타났다. 또한 과학영재학교 교사들이 수업시간에 주로 사용하는 수업방법으로 강의나 설명, 조별수업, 토론이 대다수를 차지하였고 학생들이 선호하는 수업방법으로는 강의나 설명, 토론 외에도 실험, 개인연구, 문제해결학습, 현장견학 등 다양하게 나타났다. 그리고 학생들이 선호하는 평가방법은 지필평가와 보고서평가, 실험 실습 평가의 순으로 나타났다. 본 연구의 결과 현재 개발된 교육과정 편성과 운영은 압축형 속진 교육과정의 특성이 강하여, 내용의 폭을 넓히고 접근방법을 달리하는 심화 중심 운영지침을 실현하기 어려운 것으로 나타났다. 따라서 교육과정 편제의 개선이나 영재학생 을 위한 심화중심 교육에 적합한 교육내용과 교수방법에 대한 실질적인 연구가 이루어져야 할 것이다.

• BMB Reports
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• 제47권3호
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• pp.141-148
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• 2014

Bone is an active tissue, in which bone formation by osteoblast is followed by bone resorption by osteoclasts, in a repeating cycle. Proteomics approaches may allow the detection of changes in cell signal transduction, and the regulatory mechanism of cell differentiation. LC-MS/MS-based quantitative methods can be used with labeling strategies, such as SILAC, iTRAQ, TMT and enzymatic labeling. When used in combination with specific protein enrichment strategies, quantitative proteomics methods can identify various signaling molecules and modulators, and their interacting proteins in bone metabolism, to elucidate biological functions for the newly identified proteins in the cellular context. In this article, we will briefly review recent major advances in the application of proteomics for bone biology, especially from the aspect of cellular signaling.

• Mycobiology
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• 제49권4호
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• pp.421-433
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• 2021

Morchella is a genus of fungi with the ability to concentrate Cd both in the fruit-body and mycelium. However, the molecular mechanisms conferring resistance to Cd stress in Morchella are unknown. Here, RNA-based transcriptomic sequencing was used to identify the genes and pathways involved in Cd tolerance in Morchella spongiola. 7444 differentially expressed genes (DEGs) were identified by cultivating M. spongiola in media containing 0.15, 0.90, or 1.50 mg/L Cd²⁺. The DEGs were divided into six sub-clusters based on their global expression profiles. GO enrichment analysis indicated that numerous DEGs were associated with catalytic activity, cell cycle control, and the ribosome. KEGG enrichment analysis showed that the main pathways under Cd stress were MAPK signaling, oxidative phosphorylation, pyruvate metabolism, and propanoate metabolism. In addition, several DEGs encoding ion transporters, enzymatic/non-enzymatic antioxidants, and transcription factors were identified. Based on these results, a preliminary gene regulatory network was firstly proposed to illustrate the molecular mechanisms of Cd detoxification in M. spongiola. These results provide valuable insights into the Cd tolerance mechanism of M. spongiola and constitute a robust foundation for further studies on detoxification mechanisms in macrofungi that could potentially lead to the development of new and improved fungal bioremediation strategies.

• Biomolecules & Therapeutics
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• 제23권6호
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• pp.493-509
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• 2015

Antibody-drug conjugates utilize the antibody as a delivery vehicle for highly potent cytotoxic molecules with specificity for tumor-associated antigens for cancer therapy. Critical parameters that govern successful antibody-drug conjugate development for clinical use include the selection of the tumor target antigen, the antibody against the target, the cytotoxic molecule, the linker bridging the cytotoxic molecule and the antibody, and the conjugation chemistry used for the attachment of the cytotoxic molecule to the antibody. Advancements in these core antibody-drug conjugate technology are reflected by recent approval of Adectris$^{(R)}$(anti-CD30-drug conjugate) and Kadcyla$^{(R)}$(anti-HER2 drug conjugate). The potential approval of an anti-CD22 conjugate and promising new clinical data for anti-CD19 and anti-CD33 conjugates are additional advancements. Enrichment of antibody-drug conjugates with newly developed potent cytotoxic molecules and linkers are also in the pipeline for various tumor targets. However, the complexity of antibody-drug conjugate components, conjugation methods, and off-target toxicities still pose challenges for the strategic design of antibody-drug conjugates to achieve their fullest therapeutic potential. This review will discuss the emergence of clinical antibody-drug conjugates, current trends in optimization strategies, and recent study results for antibody-drug conjugates that have incorporated the latest optimization strategies. Future challenges and perspectives toward making antibody-drug conjugates more amendable for broader disease indications are also discussed.

• 한국초등과학교육학회지:초등과학교육
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• 제23권4호
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• pp.279-286
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• 2004

The purpose of this study is to develop and apply effective teaching strategies and program for the gifted-in-science. The teaching material for the gifted students was focussed on microscope. This study consists of three steps according to the enrichment triad model of Renzulli. Firstly, the gifted students investigate and express themselves for theological backgrounds, for examples, kinds, structure and use in various ways of microscope. It is to provide basic data for the effective ideas and interest which help students have the appropriate microscopic conceptions. In the second step, it contains understanding of a term and function of microscope, making of simple microscope, principle of a statue focusing and size estimation of an outline of subjects by scientific investigation activity. Lastly, it focussed on enlargement of post-learning through investigation of relationship with inter-science and experiments using microscope. Therefore, it was concluded that this study may help teachers reconsider scientific program development for the gifted or enriched students and to be successful in the teaching-learning processes.