• Title/Summary/Keyword: electro fusion

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Flight trajectory generation through post-processing of launch vehicle tracking data (발사체 추적자료 후처리를 통한 비행궤적 생성)

  • Yun, Sek-Young;Lyou, Joon
    • Journal of Korea Society of Industrial Information Systems
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    • v.19 no.6
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    • pp.53-61
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    • 2014
  • For monitoring the flight trajectory and the status of a launch vehicle, the mission control system in NARO space center process data acquired from the ground tracking system, which consists of two tracking radars, four telemetry stations, and one electro-optical tracking system. Each tracking unit exhibits its own tracking error mainly due to multi-path, clutter and radio refraction, and by utilizing only one among transmitted informations, it is not possible to determine the actual vehicle trajectory. This paper presents a way of generating flight trajectory via post-processing the data received from the ground tracking system. The post-processing algorithm is divided into two parts: compensation for atmosphere radio refraction and multi-sensor fusion, for which a decentralized Kalman filter was adopted and implemented based on constant acceleration model. Applications of the present scheme to real data resulted in the flight trajectory where the tracking errors were minimized than done by any one sensor.

Analysis of Apoptosis on the Somatic Cell Nuclear Transfer embryos in porcine (돼지 체세포 복제 수정란의 자가 사멸 분석)

  • Ryu, Ji-Eun;Yoon, Jong-Taek
    • Journal of Embryo Transfer
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    • v.33 no.3
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    • pp.119-127
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    • 2018
  • The purpose of this study is to examined the electrofusion and activation conditions for the production of porcine somatic cell nuclear transfer (SCNT) embryos. In this study, immature oocytes were cultured in TCM-199 with and without hormones for 22 hours. Skin fibroblasts cells of porcine were transferred into the perivitelline space of enucleated in vitro matured oocytes. Cell fusion was performed with two different pulses that each one pulse (DC) of 1.1 kV/cm or 1.5 kV/cm for $30{\mu}sec$. After fusion subsequent activation were divided into three groups; non-treatment (control) and treatment with 2 mM 6-DMAP or $7.5{\mu}g/ml$ cytochalasin B for 4 hours. Transferred embryos were cultured in PZM-3 (Porcine Zygote Medium-3) in $5%\;CO_2$ and 95% air at $39^{\circ}C$ for 7 day. Apoptosis-related genes (Caspase-3, BCL-2, mTOR, and MMP-2) were analyzed by immunofluorescence staining. There was no significant difference between two different electrofusion stimuli in the cleavage rate; $64.9{\pm}4.8%$ in 1.1 kV/cm and $62.7{\pm}4.0%$ in 1.5 kV/cm. However, blastocyst formation rate (%) was significantly different among three different activation groups (no treatment, 2 mM 6-DMAP or $7.5{\mu}g/ml$ cytochalasin B) combined with electrofusion of 1.1 kV/cm. The blastocyst formation rate was $12.6{\pm}2.5$, $20.0{\pm}5.0$, and $34.9{\pm}4.3%$ in control, 2 mM 6-DMAP, and $7.5{\mu}g/ml$ cytochalasin B, respectively. Immunofluorescence data showed that expression levels of caspase-3 in SCNT embryos undeveloped to blastocyst stage were higher than those in the blastocyst stage embryos. Expression levels of Bcl-2 in blastocyst stage embryos were higher than those in the arrested SCNT embryos. These results showed that the combination of an electric pulse (1.1 kV/cm for $30{\mu}sec$) and $7.5{\mu}g/ml$ cytochalasin B treatment was effective for production of the porcine SCNT embryos.

Recent Trends of MEMS Packaging and Bonding Technology (MEMS 패키징 및 접합 기술의 최근 기술 동향)

  • Choa, Sung-Hoon;Ko, Byoung Ho;Lee, Haeng-Soo
    • Journal of the Microelectronics and Packaging Society
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    • v.24 no.4
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    • pp.9-17
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    • 2017
  • In these days, MEMS (micro-electro-mechanical system) devices become the crucial sensor components in mobile devices, automobiles and several electronic consumer products. For MEMS devices, the packaging determines the performance, reliability, long-term stability and the total cost of the MEMS devices. Therefore, the packaging technology becomes a key issue for successful commercialization of MEMS devices. As the IoT and wearable devices are emerged as a future technology, the importance of the MEMS sensor keeps increasing. However, MEMS devices should meet several requirements such as ultra-miniaturization, low-power, low-cost as well as high performances and reliability. To meet those requirements, several innovative technologies are under development such as integration of MEMS and IC chip, TSV(through-silicon-via) technology and CMOS compatible MEMS fabrication. It is clear that MEMS packaging will be key technology in future MEMS. In this paper, we reviewed the recent development trends of the MEMS packaging. In particular, we discussed and reviewed the recent technology trends of the MEMS bonding technology, such as low temperature bonding, eutectic bonding and thermo-compression bonding.

MEMS Technology for Biophotonic Applications (바이오포토닉스응용을 위한 MEMS 미세광학소자의 개발)

  • Jeong, Gi-Hun
    • Proceedings of the Optical Society of Korea Conference
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    • 2009.02a
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    • pp.387-388
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    • 2009
  • Biophotonics is an emerging area in a fusion of biology and photonics, especially in advanced bioimaging, optical biosensors, photomodulation, and biochip optical read-out, and optical manipulation. This emerging area also creates many opportunities for interdisciplinary study of biology and photonics. Micro-Electro-Mechanical-System(MEMS) is an attractive technology in miniaturizing sensors and actuactors. For last decade, it has contributed to the development for active and passive small and integrated optical components in optical communication. Recently, this technology is also merging into biology for high sensitive biosensing and high resolution and fast bioimaging in small form factor. In this talk, some key advantages of small optical components and recent biophotonic MEMS achievement will be discussed for miniaturized advanced biophotonic systems.

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Study for Field Inspection of Phase-Array Ultrasonic for Electro-fusion Joints of Polyethylene Gas Pipes (폴리에틸렌 가스배관 전기융착부 위상배열초음파검사 현장사례 연구)

  • Kil Seong-Hee;Kwon Jeong-Rock;Park Kyo-Shik
    • Journal of the Korean Institute of Gas
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    • v.10 no.2 s.31
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    • pp.61-67
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    • 2006
  • We developed the ultrasonic phased array technique for obtaining ultrasonic images of electrofusion joints of polyethylene piping. And we inspected 4 cases at fields with this technique. First case is for the 300 mm diameter polyethylene electrofusion joint by using 3.5 MHz phased array sensor, second is for the 350 mm diameter saddle electrofusion joint, third is for the 400 mm diameter electrofusion joints and the last one is for the 400 mm diameter piping joints which will be used at 300 kPa suppling pressure.

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Evaluation of the Performance of Water Electrolysis Cells and Stacks for High-Altitude Long Endurance Unmanned Aerial Vehicle (고고도 무인기용 수전해 셀 및 스택의 제작 및 성능 평가)

  • JUNG, HYE YOUNG;LEE, JUNYOUNG;YOON, DAEJIN;HAN, CHANGHYUN;SONG, MINAH;LIM, SUHYUN;MOON, SANGBONG
    • Transactions of the Korean hydrogen and new energy society
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    • v.27 no.4
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    • pp.341-348
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    • 2016
  • The experiments related on structure and water electrolysis performance of HALE UAV stack were conducted in this study. Anode catalyst $IrRuO_2$ was prepared by Adam's fusion methods as 2~3 nm nano sized particles, and the cathode catalyst was used as commercial product of Premetek. The MEA (membrane electrode assembly) was manufactured by decal methods, anode and anode catalytic layers were prepared by electro-spray. HALE stack was composed of 5 multi-cells as $0.2Nm^3/hr$ hydrogen production rate with hydrogen pressure as 10 bar. The water electrolysis performance was investigated at atmospheric pressure and temperature of $55^{\circ}C$. Best performance of HALE UAV stack was recorded as cell voltage efficiency as 86%.

Electrolyte Addition for Enhanced Wastewater Treatment by Electrolysis using Cu Electrode

  • Kim, Woo-Yeol;Yun, Chan-Young;Son, Dong-Jin;Chang, Duk;Kim, Dae-Gun;Hong, Ki-Ho
    • Journal of Electrochemical Science and Technology
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    • v.8 no.1
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    • pp.35-42
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    • 2017
  • In this study, the effect of electrolyte addition on the removal of organics and nutrients in electrochemical wastewater using a copper electrode, and the characteristics of the by-product of electrolysis were investigated. The removal of organics increased significantly as shorter reaction times upon the addition of chloride ion, and most of the electrolysis reaction was completed within 20 min. The reaction rate gradually increased in proportion to the $Cl^-$/COD ratio, whereas the highest removed mass of organic matter per mass of added electrolyte was observed at a $Cl^-$/COD ratio of 1. After the addition of electrolyte, significant removal of ammoniacal nitrogen was observed as a result of the enhanced generation of oxidizers such as hypochlorite. Excellent phosphorus removal was also achieved in a very short reaction time (within 2 min) by electro-coagulation. As the electrolysis progressed, the amount of by-product increased gradually, whereas a decrease of sludge volume index was observed after the addition of electrolyte. This indicated that the settling performance of the by-products was better, and their removal would be easily achieved.

GNSS/Multiple IMUs Based Navigation Strategy Using the Mahalanobis Distance in Partially GNSS-denied Environments (GNSS 부분 음영 지역에서 마할라노비스 거리를 이용한 GNSS/다중 IMU 센서 기반 측위 알고리즘)

  • Kim, Jiyeon;Song, Moogeun;Kim, Jaehoon;Lee, Dongik
    • IEMEK Journal of Embedded Systems and Applications
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    • v.17 no.4
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    • pp.239-247
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    • 2022
  • The existing studies on the localization in the GNSS (Global Navigation Satellite System) denied environment usually exploit low-cost MEMS IMU (Micro Electro Mechanical Systems Inertial Measurement Unit) sensors to replace the GNSS signals. However, the navigation system still requires GNSS signals for the normal environment. This paper presents an integrated GNSS/INS (Inertial Navigation System) navigation system which combines GNSS and multiple IMU sensors using extended Kalman filter in partially GNSS-denied environments. The position and velocity of the INS and GNSS are used as the inputs to the integrated navigation system. The Mahalanobis distance is used for novelty detection to detect the outlier of GNSS measurements. When the abnormality is detected in GNSS signals, GNSS data is excluded from the fusion process. The performance of the proposed method is evaluated using MATLAB/Simulink. The simulation results show that the proposed algorithm can achieve a higher degree of positioning accuracy in the partially GNSS-denied environment.

In Vitro Development of Bovine Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts (태아 섬유아세포로 재구성된 핵치환 소 수정란의 체외발달)

  • Koo, D.B.;Choi, Y.H.;Park, J.S.;Kim, H.N.;Kang, Y.K.;Lee, C.S.;Han, Y.M.;Park, H.D.;Lee, K.K.
    • Korean Journal of Animal Reproduction
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    • v.24 no.4
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    • pp.407-417
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    • 2000
  • The present study was to examine effects of various electrical stimulus treatments used for electro-fusion on the preimplantation development of bovine nuclear transfer (NT) embryos with fetal fibroblast cells. Fetal fibroblast cells were isolated from one fetus at day 45 of gestation in Holstein cow, and passaged 3 to 4 times before being transferred into enucleated oocytes. Single fibroblast cells were individually placed into the perivitelline space of enucleated oocytes by using a micromanipulator. At first, the fusion and developmental rates of reconstructed oocytes were compared between different electric stimulation conditions. When fusion of the reconstructed oocyte was induced by different electric pulse periods (15, 30 and 45 $\mu$sec) at a DC pulse of 1.8 kV/cm, 15 (45.5%, 120/264) or 30 $\mu$ sec group (43.9%, 106/241) showed a higher fusion rate than 45 $\mu$sec group (23.2%, 58/250, P<0.05). However, no difference was detected in the development rate of the fused oocytes to blastocysts between groups. Next experiment was to examine the effects of different electrical field strengths (1.5, 1.8 and 2.1 kV/cm) for 15 $\mu$sec at electrofusion on in vitro development of the NT embryos. As results, there was no difference in the fusion and developmental rates of the NT embryos between electrical strength (P>0.05). Finally, developmental competence of bovine NT embryos with somatic cells was compared with IVF-derived embryos. Of enucleated oocytes fused with fibroblast cells, 27.4% (75/274) developed to the blastocyst stage, which is similar to that (24.5%, 58/237) of IVF-derived embryos. However, mean nuclei number of NT blastocysts was smaller than that of IVF-derived blastocysts. Thus, we have established an optimal condition (1.8 kV/cm, 15 $\mu$sec) for electric fusion of bovine NT oocytes with somatic cells. The present study indicates that bovine reconstructed embryos with somatic cells normally develop to blastocyst stage in vitro, although having smaller nuclei numbers of blastocysts as compared to IVF-derived embryos.

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Effect of Groove Conditions on the Mechanical Properties of Welds Produced by the Combined Welding Process of Flux Cored Arc and Electro Gas in EH36 TMCP Steel Plate for Hull Structures (선체구조용 EH36 TMCP 후판의 FCAW 및 EGW 조합 용접부 기계적 성질에 미치는 개선조건의 영향)

  • Kim, Kihyuk;Kim, Kiwon;Sim, Hoseop;Bae, Kangho;Hong, Hyunuk;Park, Byunggyu
    • Journal of Welding and Joining
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    • v.33 no.5
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    • pp.35-40
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    • 2015
  • Characterization of microstructures and mechanical properties of 83mm thickness EH36-TM welds produced by the combined flux cored arc (FCA) and electro gas (EG) welding processes has been studied with the two different groove conditions, single-V (SV) and double-V (DV) bevels. The welding consumables used for FCA and EG welding processes were ASME/AWS A5.29 (E81T1-K2) and A5.26 (EG72T), respectively. Experimental results showed that all the mechanical properties of welds such as tensile property, CVN toughness and Vickers hardness met IACS requirements. The tensile strength of EG welded plates were reduced by approximately 4% (DV: 3.8%, SV: 4.2%) compared to the base metal. The hardness value of SV-beveled weld metal was slightly lower than that of DV-beveled one. There were no significant differences as per welding groove conditions except for the weld metal. In addition, at the fusion line, the toughness of SV condition was 20J lower and the weld metal was 40J lower than DV condition, respectively. On the basis of microstructural analysis, grain boundary ferrite (GBF) structures for SV condition were 2 times higher volume fraction than for DV condition and their packet sizes were coarsened to almost double. It was thus suggested that the GBF volume fractions and packet sizes in the weld metal of EH36-TM steel plates are the most important factors affecting the mechanical properties of the combined FCA and EG welded joint. Nevertheless, all the results of welds with both DV and SV conditions were found to be excellent.