• Asian-Australasian Journal of Animal Sciences
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• v.17 no.12
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• pp.1641-1646
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• 2004

The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploidembryonic stem (ES) cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR, cloned into $pCR^{(R)}$ 2.1 $TOPO^{(R)}$ vector and constructed in pCMV-Tag4C vector. Mammalian expression plasmid containing human resistin was transfected into D3-GL ES cells by Lipofectamine 2,000, and then after 10-12 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 $\mu$sec (fusion rate: 2,114/2,256, 93.5%) and cultured up to the blastocyst stage (development rate: 1,862/2,114, 94.6%). The selected 15-20 ES cells were injected into tetraploid blastocysts, and then transferred into the uteri of E 2.5 d pseudopregnant recipient mice. To investigate the gestation progress, two E 19.5 mused fetuses were recovered by Cesarean section of which one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, our findings demonstrate that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mice for the rapid analysis of gene function in vivo.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.47 no.1
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• pp.67-75
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• 2010

This paper presents localization algorithm for mobile robot in outdoor environment. Outdoor environment includes the uncertainty on the ground. Magnetic sensor or IMU(Inertial Measurement Unit) has been used to estimate robot's heading angle. Two sensor is unavailable because mobile robot is electric car affected by magnetic field. Heading angle estimation algorithm for mobile robot is implemented using gyro sensor module consisting of 1-axis gyro sensors. Localization algorithm applied Extended Kalman filter that utilized GPS and encoder, gyro sensor module. Experiment results show that proposed localization algorithm improve considerably localization performance of mobile robots.

• Journal of the Korean Society for Railway
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• v.20 no.3
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• pp.356-364
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• 2017

The process of inspecting electric railway vehicles is complicated and these vehicles accompany a risk of safety accidents. This developed system will be a great help in simplifying the shunting process and be very useful in terms of ensuring safety and providing user convenience. In this paper, the results of performance evaluation tests are studied on a movable catenary system for railway vehicles that secured mechanical durability, convenience, and operator safety by applying a specific rigid bar catenary of an existing mobile train line. We presented an analysis of the basic characteristics for site installation including sorting. In conclusion, this developed system was obtained in good results through durability test, durable mechanical load test and safety test in require specifications.

• Journal of Digital Convergence
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• v.16 no.11
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• pp.185-199
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• 2018

The purpose of this study is to derive the core technologies of VR using patent analysis and to explore the direction of social and public interest in VR using news analysis. In Study 1, we derived keywords using the frequency of words in patent texts, and we compared by company, year, and technical classification. Netminer, a network analysis program, was used to analyze the IPC codes of patents. In Study 2, we analyzed news articles using T-LAB program. TF-IDF was used as a keyword selection method and chi-square and association index algorithms were used to extract the words most relevant to VR. Through this study, we confirmed that VR is a fusion technology including optics, head mounted display (HMD), data analysis, electric and electronic technology, and found that optical technology is the central technology among the technologies currently being developed. In addition, through news articles, we found that the society and the public are interested in the formation and growth of VR suppliers and markets, and VR should be developed on the basis of user experience.

• Transactions of the Korean hydrogen and new energy society
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• v.33 no.3
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• pp.247-254
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• 2022

Slush hydrogen containing liquid and solid hydrogen is expected to achieve zero boil-off by suppressing boil-off gas because heat of fusion for solid absorbe the heat ingress from atmosphere. In this paper, quantitative analysis on storage cost considering specific energy consumption between 1,000 m³ class liquid hydrogen storage system with re-liquefaction and slush hydrogen storage system during equivalent zero boil off period. Even though approximately 50% of total storage capacity should be converted into solid phase during the initial cargo bunkering, total energy consumption to convert into slush hydrogen is relatively 25% less than re-liquefaction energy for boil off hydrogen during zero boil off period. That's because energy consumption of slush phase change take up only 1.8% of liquefaction energy. moreover, annual revenue requirement including CAPEX, OPEX and electric cost for slush hydrogen storage could be more reduced approximately 32.5% than those of liquid hydrogen storage and specific energy storage cost ($/kg-H₂) could also be lowered by about 41.7% compared with liquid hydrogen storage.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.12
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• pp.3976-3990
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• 2022

Currently, cross-attention is widely used in Siamese trackers to replace traditional correlation operations for feature fusion between template and search region. The former can establish a similar relationship between the target and the search region better than the latter for robust visual object tracking. But existing trackers using cross-attention only focus on rich semantic information of high-level features, while ignoring the appearance information contained in low-level features, which makes trackers vulnerable to interference from similar objects. In this paper, we propose a Multi-level Cross-attention Siamese network(MCSiam) to aggregate the semantic information and appearance information at the same time. Specifically, a multi-level cross-attention module is designed to fuse the multi-layer features extracted from the backbone, which integrate different levels of the template and search region features, so that the rich appearance information and semantic information can be used to carry out the tracking task simultaneously. In addition, before cross-attention, a target-aware module is introduced to enhance the target feature and alleviate interference, which makes the multi-level cross-attention module more efficient to fuse the information of the target and the search region. We test the MCSiam on four tracking benchmarks and the result show that the proposed tracker achieves comparable performance to the state-of-the-art trackers.

• Applied Chemistry for Engineering
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• v.33 no.4
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• pp.343-351
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• 2022

Lithium-ion batteries (LIBs) are considered promising energy storage devices with good performance such as high energy density, slow self-discharge rate, high rate charge capacity, and long battery life. However, the application of these LIBs in the high-energy density electric vehicle and large device industries poses a major safety problem. In order to solve this problem, developing a material having high thermal stability and intrinsic safety is the ultimate solution for improving the stability and electrochemical performance of LIBs. This review introduced a surface modification technology of a separator to overcome the stability problem of a commercial separator, and summarized and summarized the research trends using the modified separator for a lithium-ion battery. Based on this, the future prospects for the separator development by surface modification were discussed.

• Journal of Electrochemical Science and Technology
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• v.14 no.2
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• pp.162-173
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• 2023

Aqueous zinc-ion batteries are considered as promising alternatives to lithium-ion batteries for energy storage owing to their safety and cost efficiency. However, their lifespan is limited by the irreversibility of Zn anodes because of Zn dendrite growth and side reactions such as the hydrogen evolution reaction and corrosion during cycling. Herein, we present a strategy to restrict direct contact between the Zn anode and aqueous electrolyte by fabricating a protective layer on the surface of Zn foil via phosphidation method. The Zn₃(PO₄)₂ protective layer effectively suppresses Zn dendrite growth and side reactions in aqueous electrolytes. The electrochemical properties of the Zn₃(PO₄)₂@Zn anode, such as the overpotential, linear polarization resistance, and hydrogen generation reaction, indicate that the protective layer can suppress interfacial corrosion and improve the electrochemical stability compared to that of bare Zn by preventing direct contact between the electrolyte and the active sites of Zn. Remarkably, MnO₂ Zn₃(PO₄)₂@Zn exhibited enhanced reversibility owing to the formation a stable porous layer, which effectively inhibited vertical dendrite growth by inducing the uniform plating of Zn²⁺ ions underneath the formed layer.

• The Korean Journal of Nuclear Medicine Technology
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• v.27 no.1
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• pp.47-54
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• 2023

Purpose PET-CT imaging require an appropriate quality assurance system to achieve high efficiency and reliability. Quality control is essential for improving the quality of care and patient safety. Currently, there are performance evaluation methods of UN2-1994 and UN2-2001 proposed by NEMA and IEC for PET-CT image evaluation. In this study, we compare phantom images with the same experiments before and after PET-CT 3D normalization and well counter correction and evaluate the usefulness of quality control. Materials and methods Discovery 690 (General Electric Healthcare, USA) PET-CT equiptment was used to perform 3D normalization and well counter correction as recommended by GE Healthcare. Based on the recovery coefficients for the six spheres of the NEMA IEC Body Phantom recommended by the EARL. 20kBq/㎖ of ¹⁸F was injected into the sphere of the phantom and 2kBq/㎖ of ¹⁸F was injected into the body of phantom. PET-CT scan was performed with a radioacitivity ratio of 10:1. Images were reconstructed by appliying TOF+PSF+TOF, OSEM+PSF, OSEM and Gaussian filter 4.0, 4.5, 5.0, 5.5, 6.0, 6,5 mm with matrix size 128×128, slice thickness 3.75 mm, iteration 2, subset 16 conditions. The PET image was attenuation corrected using the CT images and analyzed using software program AW 4.7 (General Electric Healthcare, USA). The ROI was set to fit 6 spheres in the CT image, RC (Recovery Coefficient) was measured after fusion of PET and CT. Statistical analysis was performed wilcoxon signed rank test using R. Results Overall, after the quality control items were performed, the recovery coefficient of the phantom image increased and measured. Recovery coefficient according to the image reconstruction increased in the order TOF+PSF, TOF, OSEM+PSF, before and after quality control, RCmax increased by OSEM 0.13, OSEM+PSF 0.16, TOF 0.16, TOF+PSF 0.15 and RCmean increased by OSEM 0.09, OSEM+PSF 0.09, TOF 0.106, TOF+PSF 0.10. Both groups showed a statistically significant difference in Wilcoxon signed rank test results (P value<0.001). Conclusion PET-CT system require quality assurance to achieve high efficiency and reliability. Standardized intervals and procedures should be followed for quality control. We hope that this study will be a good opportunity to think about the importance of quality control in PET-CT

• Journal of rehabilitation welfare engineering & assistive technology
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• v.10 no.4
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• pp.321-328
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• 2016

In this paper, we propose a walk status method which can be fused with conventional walk intention method to improve convenience of an electric assistive walking system for elder people with restricted walking capabilities. The system uses a handlebar as a trigger and regards grabbing a handlebar as expressing will to walk. And the system uses a user's linear velocity and angular velocity as linear velocity and angular velocity of a system, checked by laser range finder. To achieve this, we propose a method to find a virtual central point of a human body by estimating a central point between two legs. The experiments are carried out by comparing user's linear velocity and angular velocity, and system's linear velocity and angular velocity. The results show that the error of linear velocity and angular velocity between a user and a system are 1% and 2.77%, which means the linear velocity and angular velocity of a user can be applied to a system. And it is confirmed that the proposed fusion method can prevent a user from being dragged by an assistive walking system or a malfunction caused by lack of experience