• Title/Summary/Keyword: efficient translation

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Construction of Yeast Vectors Potentially Useful for Expression of Eukaryotic Genes as ${\beta}$-galactosidase Fusion Proteins

  • Chung, Kyung-Sook;Choi, Won-Ja;Lee, Hee-Won;Kim, Kyu-Won;Yoo, Hyang-Sook
    • BMB Reports
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    • v.29 no.4
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    • pp.359-364
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    • 1996
  • By both in vitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene (PGK) promoter DNA and insertion of the leu2-d gene, we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine (G) to adenine (A) change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6~7 times elevated expression of the adjacent eukaryotic gene, and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene. When expression of the AIDS virus HIV1-gagP17 gene in a lacZ fusion form was examined with this new vector, a 15 times higher level of expression than that from the original PGK promoter was observed. Northern and Southern analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA. Thus, the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast.

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An Efficient Address Mapping Table Management Scheme for NAND Flash Memory File System Exploiting Page Address Cache (페이지 주소 캐시를 활용한 NAND 플래시 메모리 파일시스템에서의 효율적 주소 변환 테이블 관리 정책)

  • Kim, Cheong-Ghil
    • Journal of Digital Contents Society
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    • v.11 no.1
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    • pp.91-97
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    • 2010
  • Flash memory has been used by many digital devices for data storage, exploiting the advantages of non-volatility, low power, stability, and so on, with the help of high integrity, large capacity, and low price. As the fast growing popularity of flash memory, the density of it increases so significantly that its entire address mapping table becomes too big to be stored in SRAM. This paper proposes the associated page address cache with an efficient table management scheme for hybrid flash translation layer mapping. For this purpose, all tables are integrated into a map block containing entire physical page tables. Simulation results show that the proposed scheme can save the extra memory areas and decrease the searching time with less 2.5% of miss ratio on PC workload and can decrease the write overhead by performing write operation 33% out of total writes requested.

Cliche Analysis for English-Korean Interpretation and Translation Training : Mainly on Shakespeare's Works Texts (영·한 통번역 교육을 위한 클리셰(cliche) 분석 : 셰익스피어 극 텍스트를 중심으로)

  • You, Seon-Young
    • The Journal of the Korea Contents Association
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    • v.15 no.11
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    • pp.626-634
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    • 2015
  • The purpose of this study was to analyze the cliche for English-Korean interpretation and translation training with special reference to the cliche based on Shakespeare's works texts. The term of 'idioms' are generally used as figurative expressions instead of the term of 'cliche'. Thus, cliches must be reinterpreted in the lexicon that are used in useful expressions. Cliches are often idioms. Idioms are figurative phrases with an implied meaning; the phrase is not to be taken literally. This causes difficulty when translating to another language because the meaning may not be understood by people within that culture. Cliches are figurative or literal expressions and are overused expressions. Consequently, the cliches are distinguished from the idioms by the transparent meanings. This study was examined based on the cliches shown in Shakespeare's works texts. After all, anyone who wants to become an efficient English learners, interpretor and translator should be familiar with cliches. They had better use the cliche in English learning site. I hope this study will be helpful even a bit to his attempt.

Automatic Compiler Generator for Visual Languages using Semantic Actions based on Classes (클래스 기반의 의미수행코드 명세를 이용한 시각언어 컴파일러 자동 생성)

  • 김경아
    • Journal of Korea Multimedia Society
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    • v.6 no.6
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    • pp.1088-1099
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    • 2003
  • The syntax-directed translation using semantic actions is frequently used in construction of compiler for text programming languages. it is very useful for the language designers to develop compiler back-end using a syntax structure of a source programming language. Due to the lack of the integrated representation method for a parse tree node and modeling method of syntax structures, it is very hard to construct compiler using syntax-directed translation in visual languages. In this Paper, we propose a visual language compiler generation method for constructing a visual languages compiler automatically, using syntax-directed translation. Our method uses the Picture Layout Grammar as a underlying grammar formalism. This grammar allows our approach to generate parser efficiently u sing And-Or-Waiting Graph and encapsulating syntax definition as one unit. Unlike other systems, we suggest separating the specification and the generation of semantic actions. Because of this, it provides a very efficient method for modification.

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The Efficient Scenario of Solving NAT Traversal in the IMS (IMS에서 효율적인 NAT Traversal 해결 시나리오)

  • Han, Seok-Jun;Lee, Jae-Oh;Kang, Seung-Chan
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.4
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    • pp.1935-1941
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    • 2013
  • We can use NAT(Network Address Translation) technology to solve the lack of IP address. The problem of NAT traversal is happened when the filtering characteristics of NAT remove the packet that has no binding in the address translation table of NAT. There were many kinds of way to solve these problems by using additional device. Lately, network market is changed into integrating wired and wireless network by the IMS(IP Multimedia Subsystem). The IMS integrates to control network of wired and wireless network, has emerged to control convergence network effectively. Lately, the additional devices like IBCF(Interconnection Border Control Function) and IBGF(Interconnection Border Gateway Function) are used to solve the NAT traversal problem in the IMS. In this paper, we propose the solution of NAT traversal using P-CSCF without any additional equipment in the IMS.

Block Associativity Limit Scheme for Efficient Flash Translation Layer (효율적인 플래시 변환 계층을 위한 블록 연관성 제한 기법)

  • Ok, Dong-Seok;Lee, Tae-Hoon;Chung, Ki-Dong
    • Journal of KIISE:Computing Practices and Letters
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    • v.16 no.6
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    • pp.673-677
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    • 2010
  • Recently, NAND flash memory has been widely used in embedded systems, personal computers, and server systems because of its attractive features, such as non-volatility, fast access speed, shock resistance, and low power consumption. Due to its hardware characteristics, specifically its 'erase-before-write' feature, Flash Translation Layer is required for using flash memory like hard disk drive. Many FTL schemes have been proposed, but conventional FTL schemes have problems such as block thrashing and block associativity problem. The KAST scheme tried to solve these problems by limiting the number of associations between data block and log block to K. But it has also block thrashing problem in random access I/O pattern. In this paper, we proposed a new FTL scheme, UDA-LBAST. Like KAST, the proposed scheme also limits the log block association, but does not limit data block association. So we could minimize the cost of merge operations, and reduce merge costs by using a new block reclaim scheme, log block garbage collection.

An Analysis of Graphing Domain in the Sixth and the Seventh Curriculum Textbooks (6차와 7차 교과서 분석을 통한 그래프 지도 방안)

  • 송정화;권오남
    • School Mathematics
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    • v.4 no.2
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    • pp.161-192
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    • 2002
  • This paper investigated the teaching and teaming of contents-related graphing in Korean secondary textbooks and suggested the improved methods of graph instruction through this analysis. reification-the case of function, In Harel, G., Dubinsky(Eds.), The Concept of Function : Aspects of Epistemology and Pedagogy Textbooks are analyzed from the viewpoint of the proportion of graphing contents, their sequencing, the proportion of each domain in graphing activities (interpretation vs. construction, quantitative vs. qualitative aspect, local vs. global aspect) and tasks (prediction, translation, scaling), and the difference in the graphing contents between the sixth and the seventh curriculum. This analysis demonstrates that graphing contents are increasing in textbooks, therefore the high school textbooks appear in almost every content area. The graphing activities concentrate on the construction, the quantitative aspects, and the local aspects, and are gradually focusing on the interpretation and global aspects of high school textbooks. Furthermore, most of graphing tasks favor translation. In contrast, the current seventh curriculum includes a balance of interpretation and construction activities and has more global aspects than the sixth curriculum based textbooks; however, the qualitative approach still rarely appears. For the graphing tasks, translation is still prevalent, but the importances of prediction tasks based on graph have increased in comparison with the sixth curriculum textbooks. Further, the seventh curriculum based textbooks are designed to stimulate more dynamic graphing instruction by introducing new tools such as graphing calculators and computer software. We suggest that the qualitative and global aspects should be emphasized in early graph instruction, a variety of graph activities in realistic contexts should be performed, and educational technology such as graphing calculator and computer can be efficient to implement these ideas.

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OnDot: Braille Training System for the Blind (시각장애인을 위한 점자 교육 시스템)

  • Kim, Hak-Jin;Moon, Jun-Hyeok;Song, Min-Uk;Lee, Se-Min;Kong, Ki-sok
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.6
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    • pp.41-50
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    • 2020
  • This paper deals with the Braille Education System which complements the shortcomings of the existing Braille Learning Products. An application dedicated to the blind is configured to perform full functions through touch gestures and voice guidance for user convenience. Braille kit is produced for educational purposes through Arduino and 3D printing. The system supports the following functions. First, the learning of the most basic braille, such as initial consonants, final consonant, vowels, abbreviations, etc. Second, the ability to check learned braille by solving step quizzes. Third, translation of braille. Through the experiment, the recognition rate of touch gestures and the accuracy of braille expression were confirmed, and in case of translation, the translation was done as intended. The system allows blind people to learn braille efficiently.

The case study for Implementation and verification of Dynamic NAT and PAT (동적 NAT과 PAT의 구현과 검증 사례연구)

  • Kim, No-Whan
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.10
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    • pp.1131-1138
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    • 2015
  • As the size of the internet market grows rapidly, the number of IPv4 addresses available is being exhausted, while transition to IPv6 is being delayed. As the best alternative solution, Network Address Translation(NAT) scheme is being used. It connects the public internet network with the private internet network in order to reduce the waste of IPv4 addresses space. The purpose of this paper is to study the effective example of network based on common virtual network using Packet Tracer with topology designed rather than usual theoretical approach in Dynamic NAT and PAT, which allows more efficient use of address space.

Effects of different target sites on antisense RNA-mediated regulation of gene expression

  • Park, Hongmarn;Yoon, Yeongseong;Suk, Shinae;Lee, Ji Young;Lee, Younghoon
    • BMB Reports
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    • v.47 no.11
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    • pp.619-624
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    • 2014
  • Antisense RNA is a type of noncoding RNA (ncRNA) that binds to complementary mRNA sequences and induces gene repression by inhibiting translation or degrading mRNA. Recently, several small ncRNAs (sRNAs) have been identified in Escherichia coli that act as antisense RNA mainly via base pairing with mRNA. The base pairing predominantly leads to gene repression, and in some cases, gene activation. In the current study, we examined how the location of target sites affects sRNA-mediated gene regulation. An efficient antisense RNA expression system was developed, and the effects of antisense RNAs on various target sites in a model mRNA were examined. The target sites of antisense RNAs suppressing gene expression were identified, not only in the translation initiation region (TIR) of mRNA, but also at the junction between the coding region and 3' untranslated region. Surprisingly, an antisense RNA recognizing the upstream region of TIR enhanced gene expression through increasing mRNA stability.