• 제목/요약/키워드: ear type

검색결과 244건 처리시간 0.033초

Metabolic Flux Distribution for $\gamma$-Linolenic Acid Synthetic Pathways in Spirulina platensis

  • Meechai Asawin;Pongakarakun Siriluk;Deshnium Patcharaporn;Cheevadhanarak Supapon;Bhumiratana Sakarindr
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제9권6호
    • /
    • pp.506-513
    • /
    • 2004
  • Spirulina produces $\gamma$-linolenic acid (GLA), an important pharmaceutical substance, in a relatively low level compared with fungi and plants, prompting more research to improve its GLA yield. In this study, metabolic flux analysis was applied to determine the cellular metabolic flux distributions in the GLA synthetic pathways of two Spiru/ina strains, wild type BP and a high­GLA producing mutant Z19/2. Simplified pathways involving the GLA synthesis of S. platensis formulated comprise of photosynthesis, gluconeogenesis, the pentose phosphate pathway, the anaplerotic pathway, the tricarboxylic cycle, the GLA synthesis pathway, and the biomass syn­thesis pathway. A stoichiometric model reflecting these pathways contains 17 intermediates and 22 reactions. Three fluxes - the bicarbonate (C-source) uptake rate, the specific growth rate, and the GLA synthesis rate - were measured and the remaining fluxes were calculated using lin­ear optimization. The calculation showed that the flux through the reaction converting acetyl­CoA into malonyl-CoA in the mutant strain was nearly three times higher than that in the wild­type strain. This finding implies that this reaction is rate controlling. This suggestion was sup­ported by experiments, in which the stimulating factors for this reaction $(NADPH\;and\;MgCl_{2})$ were added into the culture medium, resulting in an increased GLA-synthesis rate in the wild type strain.

제2형 당뇨환자의 개인별 맞춤영양교육의 효과 평가 (Individual Diabetes Nutrition Education Can Help Management for Type II Diabetes)

  • 우예지;이현숙;김화영
    • Journal of Nutrition and Health
    • /
    • 제39권7호
    • /
    • pp.641-648
    • /
    • 2006
  • This study was designed to evaluate the effect of the individual nutrition education for type 2 diabetes who participate the diabetes buffet. The subjects were 66 patients and divided into education (n=34) and control groups (n=32). The mean age of education and control groups were 59.8 and 56.6 years old, respectively. There were no differences in age and body mass index (BMI) between two groups. Initial glycosylated hemoglobin (HbA1c), post prandial plasma glucose (PP2), total plasma cholesterol, and blood pressure were not different between two groups. But fasting blood sugar (FBS) was higher in education group than in control group. On completion of the study, the education group showed significant decreases in body weight, BMI, FBS and PP2, however, the control group showed no changes in body weight, BMI and PP2, and showed a significant increase in FBS. Initial calorie and protein intakes of the education group did not meet the prescribed amount, however, mineral and vitamin intakes were higher than estimated average requirement (EAR). By the end of study, calorie and protein intakes were significantly increased to meet the prescription. In order to evaluate the effect of diet education, awareness of calorie requirement was used as an index of understanding diet prescription. The degree of awareness of calorie requirement was dependent on age: younger patients showed higher awareness than older subjects. The subject who showed better understanding of diet prescription showed lower levels of HbA1c, FBS, and PP2 at the end of the experiment period. The results of this study clearly show that individual diabetes diet education is effective to make the patient understand their diet prescription, and is effective to control body weight and blood sugar level. Awareness of calorie requirement could be used as an index of understanding of prescribed diet. Since age is an important variable to determine the awareness of calorie requirement, different strategies of nutrition education should be developed for different age groups, especially patients over 70 years old.

방풍통성산(防風通聖散)의 문헌(文獻) 및 형상의학적(形象醫學的) 고찰(考察) (Documentational Study and Observation from the View of Hyungsang Medicine on Bangpungtongseong-san)

  • 석민희;김준홍;이용태
    • 동의생리병리학회지
    • /
    • 제22권1호
    • /
    • pp.51-59
    • /
    • 2008
  • The following conclusions were obtained from the studies on Bangpuntongseongsan both from the documents and Hyungsang medicine. Bangpungtongseong-san was created by Yu Wan-so to relieve both interior and exterior of disease from the pathogenic fire, and it cures wind syndrome and dry syndrome. Bangpungtongseong-san is of light herbal combination and it works in the upper part of the body and is mainly applied to skin disease. Perspiration without harming the exterior and purgation without hurting the interior shows that it is not a severe prescription belonging to meditation therapy. It is mostly used for curing the disease of internal heat caused by over drinking and consuming heavy food, and it has special relationship with Yangmyung meridian. It is mentioned in the chapters of spirit, head, face, eye, ear, nose, throat, skin, hair, prescription, wind, dryness, fire, internal damage, epidemic infectious disease, carbuncle and cellulitis, ulceration, and pediatrics of ${\ulcorner}$Donguibogam${\lrcorner}$. It is usually applied to those who belong to Yangmyung type of the six meridian types or wind type, who has excessive heat, people with red complexion, reddened nose, pimples over the face and nose, coarse heel, loss of hair due to wind-heat, and to those who tend to have dandruff. Through examination over the cases treated with Hyungsan medicine, Bangpungtongseong-san was found efficacious in bloodshot eyes, brandy nose, loss of hair, various skin problems, tetanus, acute alcoholism, paralysis of hand and foot, deafness, and tinnitus.

적산온도를 활용한 중북부지역 찰옥수수 적정파종기 설정 (Determination of Adaptable Sowing Dates of Waxy Corn Using Growing Degree Days in the Central Northern Area of Korea)

  • 심강보;이종기;구본일;신명나;윤성탁
    • 한국작물학회지
    • /
    • 제64권3호
    • /
    • pp.269-277
    • /
    • 2019
  • 파종시기를 달리하여 옥수수 생육 및 수량성을 비교하고 출사기, 출사후 등숙기까지 적산온도를 활용하여 중북부지역에서 적합한 옥수수 파종시기를 구명하고자 2017~2018년 2년에 걸쳐 수행하였으며, 그 결과를 요약하면 다음과 같다. 1. 파종기가 빠를수록 토양온도가 낮아 옥수수 유묘 출현기간이 길었으며, 파종시 기별 평균 유묘 출현소요일수는 조생형인 미백2호가 9.1일, 중생형인 일미찰이 9.5일, 만생형인 찰옥4호가 10.1일 소요되어 조생형일수록 유묘출현소요일수가 짧았다. 2. 지온과 유묘 출현일수는 부의 상관관계를 나타내었는데, 지온이 낮은 4월에는 유묘 출현일수가 16~22일, 지온이 높은 5월에는 9~15일 정도로 감소하였다. 3. 파종기에 따른 경장, 이삭길이, 이삭폭은 수량과 정의 상관관계를 나타내었으며 조생형인 미백2호는 5월 15일, 중생형인 일미찰은 5월 25일, 만생형인 찰옥4호는 6월 15일 파종에서 각각 수량이 가장 높아 중북부지역의 평년기온 조건에서 생태형별 적정 파종기로 판단할 수가 있었다. 4. 동일 파종조건에서 출사 후 26일 까지 품종 간 유효적산 온도 차이는 적었으나 만생형 품종일수록 성숙기까지 소요적산온도가 적었는데 출사 후 26일까지 유효적산온도는 조생형인 미백2호가 $975^{\circ}C$, 만생형인 찰옥4호가 $604^{\circ}C$이었다. 5. 중북부지역에서 찰옥수수를 재배할 경우 생태형에 따른 품종의 파종지표로 유효적산온도를 활용할 경우 조생형인 미백2호는 출사 후 26일까지 적산온도, 중생형인 일미찰은 전 생육기간 적산온도, 만생형인 찰옥4호는 출사기까지 적산온도가 적합하였다. 6. 중북부지역(강원도, 경기북부)에서 생태형에 따른 찰옥수수를 재배 할 경우 파종기부터 등숙기까지 평균 적산온도는 약 $2,400^{\circ}C$, 평균 출사일수는 65일 이상 조건에서 10 a 당 1,200 kg 수준의 수량성 확보가 가능하였다.

대학 신입생의 스트레스 민감 여부에 따른 영양상태 (The Nutritional Status by Stress on Freshmen of University)

  • 이영희;이승교;원향례
    • 한국지역사회생활과학회지
    • /
    • 제17권4호
    • /
    • pp.81-95
    • /
    • 2006
  • This study was made to find out how stress affect on nutrition status of the college freshmen who were experiencing physical growth and development as well as drastic emotional change. 400 male and female freshmen in 4 year colleges were surveyed respectively through the health check-up procedure for college entrance in February, In order to find out the stress in each group frustration, deprivation, lack of self efficacy, type A behavior and anxiety response were surveyed through 10 questions with total 40 points by assigning 4 points for each question. Diet Status was expressed by DDS (Dietary Diversity Score by 5 food groups) and DVS(Dietary Variety Score). 24-hrs recall method was used to find out the quantity of daily nutrient of EAR(estimated adquacy ratio) by KDRIs(Korean Dietary Recommended Intakes). Nutrition level was analyzed by Can-Pro for professionals (Korea Nutrition Association). And for the quality intake, percentage was calculated and MAR(Mean Adequacy Ratio) were produced. Highest point was obtained in the stress of anxiety with the total 40 score of 30.20, and the scores were 29.79, 28.67, and 28.39 for deprivation, type A behavior and frustration respectively. There was no difference of blood components in accordance with stress type. Stress type was divided into less sensitive group and highly sensitive one and the relationship with the blood nutrient status was observed. The difference of blood component and blood pressure in sensitive and highly sensitive groups was observed in deprivation and anxiety. The index of blood pressure(p<0.05), hemoglobin(p<0.01), HDL-cholesterol(p<0.05), and Fe(p<0.05) was high in the deprivation of sensitive group. Blood pressure and hemoglobin was high in type A of sensitive group(p<0.05). And the contents of blood triglyceride was high in the anxiety of sensitive group(p<0.001) The result of nutrition intake analysis according to stress type showed that there was low intake for energy, riboflavin, and niacin. When the degree of deprivation was high there was a lack of riboflavin intake and there was no significant difference of nutrition intake in lack of self efficacy, type A behavior and anxiety response. Thus, it is necessary for colleges to educate the students to maintain mental stability through various programs and activities after catching a kind and extent of the stress college students we meeting with like the confusion of value system, open heterosexual relationship, and the employment difficulties linked with political uncertainty and economic recession.

  • PDF

수정된 열교환법에 의한 sapphire 단결정의 성장 : I. 사각단면 단결정의 제조 (Sapphire single crystal growth by the modified heat exchanger method : I. Preparation with the square cross-section)

  • 이민상;김성균;김동익;진영철
    • 한국결정성장학회지
    • /
    • 제8권1호
    • /
    • pp.1-9
    • /
    • 1998
  • 본 연구에서는 냉각매체로서 물을 이용하는 수정된 열교환법을 이용하여 45$\times$45$\times$20(mm) 크기의 사각단면 형상의 sapphire 단결정의 제조가능 조건에 대하여 조사하였다. 온도에 따른 성장로 내의 압력 변화로부터 사파이어의 용융 및 응고 과정을 추적할 수 있었으며, 이로부터 sapphire 단결정은 1970~$1960^{\circ}C$에서 응고가 완료됨을 알 수 있었다. 도가니 성형시 이루어지는 '귀'의 형태는 도가니 벽면과 접촉되지 않는 '나선형태'이어야 한다. 열유출부는 융액 내의 온도구배를 지배하며 융액내의 열유속과 씨앗 결정의 흔적은 Mo 봉의 체적 변화로서 조절할 수 있었다. 기공 형성을 억제하기 위해서는 $0.2^{\circ}C$/min 이하의 발열체의 냉각속도가 요구되었다.

  • PDF

크리트 가진법을 이용한 타이어특성에 따른 로드노이즈 예측 연구 (Road Noise Prediction Based on Frequency Response Function of Tire Utilizing Cleat Excitation Method)

  • 박종호;황성욱;이상권
    • 한국소음진동공학회논문집
    • /
    • 제22권8호
    • /
    • pp.720-728
    • /
    • 2012
  • It is important for identification of noise and vibration problem of tire to consider influence of interaction between road and tire. A quantification of road noise is a challenging issue in vehicle NVH due to extremely complicated transfer paths of road noise as well as the difficulty in an experimental identification of input force from tire-road interaction. A noise caused by tire is divided into road noise(structure-borne noise) and pattern noise(air-borne noise). Pattern noise is caused by pattern shape of tire, which has larger than 500 Hz, but road noise is generated by the interactions between a tire and a vehicle body. In this paper, we define the quantitative analysis for road noise caused by interactions between tire and road parameters. For the identification of road noise, the chassis dynamometer that is equipped $10mm{\times}10mm $ square cleat in the semi-anechoic chamber is used, and the tire spindle forces are measured by load cell. The vibro-acoustic transfer function between ear position and wheel center was measured by the vibro-acoustic reciprocity method. In this study three tires with different type of mechanical are used for the experiment work.

TMA로 유도된 접촉성 과민 반응 마우스 질환모델에서 해양성 광천수 도포시의 치료효과 (Effect of Brine Mineral Water on TMA-Induced Contact Hypersensitivity Reaction in the Mouse Model)

  • 김재진;김완재;심재만;최선강;권순성;김정득;이광호
    • 한국식품영양학회지
    • /
    • 제23권4호
    • /
    • pp.440-445
    • /
    • 2010
  • Effect of brine mineral water(BMW) on contact hypersensitivity reaction(CHR) was estimated using trimellitic anhydride (TMA) induced CHR in the mouse. BMW exhibited potent inhibitory activity on TMA induced CHR. BMW treatment suppressed the ear swelling, and attenuated hyper-activated lymph nodes stimulated by TMA challenge, thereby reduced their weight. The immunological index was analyzed after BMW administration in CHR. The level of serum IgE was significantly down regulated after BMW treatment. Furthermore, BMW showed inhibitory property of cytokine production. BMW treatment suppressed not only Th2 type cytokine, IL-4 but also pro-inflammatory cytokines such as TNF-${\alpha}$ and IL-6. From the histological analysis, the inflammatory context appeared by atopic dermatitis lesion after challenging with TMA are diminished by BMW treatment. Our results suggest that BMW may attenuate the development of CHR, and can be available for functional ingredient.

Genomic Tandem Quadruplication is Associated with Ketoconazole Resistance in Malassezia pachydermatis

  • Kim, Minchul;Cho, Yong-Joon;Park, Minji;Choi, Yoojeong;Hwang, Sun Young;Jung, Won Hee
    • Journal of Microbiology and Biotechnology
    • /
    • 제28권11호
    • /
    • pp.1937-1945
    • /
    • 2018
  • Malassezia pachydermatis is a commensal yeast found on the skin of dogs. However, M. pachydermatis is also considered an opportunistic pathogen and is associated with various canine skin diseases including otitis externa and atopic dermatitis, which usually require treatment using an azole antifungal drug, such as ketoconazole. In this study, we isolated a ketoconazole-resistant strain of M. pachydermatis, designated "KCTC 27587," from the external ear canal of a dog with otitis externa and analyzed its resistance mechanism. To understand the mechanism underlying ketoconazole resistance of the clinical isolate M. pachydermatis KCTC 27587, the whole genome of the yeast was sequenced using the PacBio platform and was compared with M. pachydermatis type strain CBS 1879. We found that a ~84-kb region in chromosome 4 of M. pachydermatis KCTC 27587 was tandemly quadruplicated. The quadruplicated region contains 52 protein coding genes, including the homologs of ERG4 and ERG11, whose overexpression is known to be associated with azole resistance. Our data suggest that the quadruplication of the ~84-kb region may be the cause of the ketoconazole resistance in M. pachydermatis KCTC 27587.

Enhanced Homologous Recombination in Fusarium verticillioides by Disruption of FvKU70, a Gene Required for a Non-homologous End Joining Mechanism

  • Choi, Yoon-E.;Shim, Won-Bo
    • The Plant Pathology Journal
    • /
    • 제24권1호
    • /
    • pp.1-7
    • /
    • 2008
  • Fusarium verticillioides (teleomorph Gibberella moniliformis) is associated with maize worldwide causing ear rot and stalk rot, and produces fumonisins, a group of mycotoxins detrimental to humans and animals. While research tools are available, our understanding of the molecular mechanisms associated with fungal virulence and fumonisin biosynthesis in F. verticillioides is still limited. One of the restraints that hampers F. verticillioides gene characterization is the fact that homologous recombination (HR) frequency is very low (<2%). Screening for a true gene knock-out mutant is a laborious process due to a high number of ectopic integrations. In this study, we generated a F. verticillioides mutant (SF41) deleted for FvKU70, a gene directly responsible for non-homologous end-joining mechanism, with the aim of improving HR frequency. Here, we demonstrate that FvKU70 deletion does not impact key Fverticillioides phenotypes, e.g., development, secondary metabolism, and virulence, while dramatically improving HR frequency. Significantly, we also confirmed that a high percentage (>85%) of the HR mutant strains harbor a desired mutation with no additional copy of the mutant allele inserted in the genome. We conclude that SF41 is suitable for use as a type strain when performing high-throughput gene function studies in F. verticillioides.