• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제30권1호
• /
• pp.17-24
• /
• 2004

The purpose of this study was to evaluate the stability and efficacy of biologic membrane made of freeze-dried cartilage as a barrier to facilitate guided bone regeneration in experimental non-healing bone defects in the rat mandible. Nine adult Sprague-Dawley rats (400-500g) were used in experiment. 5.0mm in diameter were created on the mandibular angle area by means of slow-speed trephine drill. In microscopic examination, dynamic immature bone forming at 2 weeks and its calcification at 4 weeks were observed. The membrane made of lyophilized cartilage taken from human costal cartilage seems to be very effective for guided bone regeneration as a biologic membrane and the scaffold for attachment of cells or local drug delivery system of growth factor, which may meet the ideal requirement of a barrier membrane and graft materials.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2006년도 춘계학술대회논문집
• /
• pp.922-929
• /
• 2006

In this study, flow characteristics of the FPN (Fountain Pen Nano-Lithography) using active membrane pumping are investigated. This FPN has integrated chamber, micro channel, and high capacity reservoir for continuous ink feed. The most important aspect in this probe provided control of fluid injection using active membrane pumping in chamber. The flow rates in channel by capillary force are theoretically analyzed, including the control of mass flow rates by deflection of membrane. The above results are compared with numerical simulations that calculated by commercial code, FLUENT. The velocity of fluid in micro channel shows linear behaviors. And the mass flows are proportional to the second order function of pumping pressure that is imposed to membrane.

• Bulletin of the Korean Chemical Society
• /
• 제20권5호
• /
• pp.556-558
• /
• 1999

Lead-selective PVC membrane electrodes based on newly synthesized bis(crown ether)s containing 18-crown-6 moiety was prepared using standard PVC membrane composition. In order to monitor lead in environmental samples by lead sensor, especially good selectivity over alkali and alkaline earth metals has to be obtained. Thus, responses of the PVC membrane prepared with new bis(crown ether)s and Fluka ionophore V (crown ether) base to various cations include lead, alkali and alkaline earth metal ions were investigated for their use as a lead sensor. The polymeric liquid membrane based on trans-1 8-crown-6 ether exhibits the best overall potentiometric performances as a lead-selective electrode in terms of a wide linear dynamic range (between 10-6 and 10-2 M, Pb2+), excellent detection limit (less than 10-6 M) and good durability within limited error. The preferences of lead over other cations, such as Ag+, Hg2+, Na+, Ca2+ and even K+ in the aforementioned electrode are much better than the Fluka ionophore V system.

• 한국전기화학회:학술대회논문집
• /
• 한국전기화학회 2005년도 춘계총회 및 학술발표회
• /
• pp.63-63
• /
• 2005

• Membrane and Water Treatment
• /
• 제11권2호
• /
• pp.131-139
• /
• 2020

The current study focused on the preparation of low-cost PVC-based adsorbing membrane. Metakaolin, as available adsorbent, was embedded into the PVC matrix via solution blending method. The as-prepared PVC/metakaolin mixed matrix membranes were characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, atomic force microscopy (AFM), pure water permeability and contact angle measurements. The results confirmed the improvement of PWP and hydrophilicity due to the presence of metakaolin in the PVC matrix. Additionally the structure of PVC membrane was changed due to the incorporation of metakaolin in the polymer matrix. The static adsorption capacity of all samples was determined through dye removal. The effect of metakaolin dosage (0-7%) and pH (4, 8, 12) on dye adsorption capacity was investigated. The results depicted that the highest adsorption capacity was achieved at pH of 4 for all samples. Additionally, adsorption data were fitted on Langmuir, Freundlich, and Temkin models to determine the appropriate governing isotherm model. Finally, the dynamic adsorption capacity of the optimum PVC/metakaolin membrane was studied using dead-end filtration cell. The dye removal efficiency was determined for pure PVC and PVC/metakaolin membrane. The results demonstrated that PVC/metakaolin mixed matrix membrane had a high adsorption capacity for dye removal from aqueous solution.

• Journal of Microbiology and Biotechnology
• /
• 제11권1호
• /
• pp.40-49
• /
• 2001

The present work presents a novel approach for the dynamic quantification of respiration rates on a small scale by using lysine-producing Corynebacterium glutamicum ATCC 21253. Cells sampeld from batch cultures at different times were incubated ina 12-ml scale bioreactor equipped with a membrane mass spectrometer. Under dynamic conditions, gas exchange across the gas-liquid phase, specific respiration rates, and RQ values were precisely measured. For this purpose, suitable mass balances were formulated. The transport coefficients for $O_2$ and $CO_2$, crucial for calculating the respiration activity, were determined as $k_La_{O2}=9.18h^{-1}$ and $k_La_{CO2}=5.10h^{-1}$ at 400 rpm. The application of the proposed method to batch cultures of C. glutamicum ATCC 21253 revealed the maximum specific respiration rates of $q_{O2}=8.4\;mmol\;g^{-1}h^{-1}\;and\;q_{CO2}=8.7\;mmol\;g^{-1}h^{-1}$ in the middle of the exponential growth phase after 5 h of cultivation. When the cells changed from growth to lysine production due to the depletion of the essential amino acids theonine, methionine, and leucine, $q_{O2}\;and\;q_{CO2}$ decreased significantly and RQ increased. The respiration data exhibited an excellent agreement with previous cultivations of the strain [13]. This confirms the potential of the developed approach to realistically reflect the metabolic activities of cells at their point of sampling. The short-term influence of added threonine, methionine, and leucine was highest during the shift from growth to lysine production, where $q_{O2}\;and\;q_{CO2}$ increased 50% within one minute after the pulse addition of these compounds. Non-growing, yet lysine-producing cells taken from the end of the batch cultivation revealed no metabolic stimulation with the addition of threonine, methionine, and leucine.

• 한국식품영양과학회지
• /
• 제13권4호
• /
• pp.459-468
• /
• 1984

The currently accepted model of membrane structure proposes a dynamic, asymmetric lipid matrix of phospholipids and cholesterol with globular proteins embedded across the membrane to various degrees. Most phospholipids are in the bilayer arrangement and also closely associated with integral membrane proteins or loosely associated with peripheral proteins. Biological functions of membrane, such as membrane-bound enzyme functions and transport systems, are influenced by the membrane physical properties, which are determined by fatty acid composition of phospholipids, polar head group composition and membrane cholesterol content. Polar and non-polar region of the phospholipid molecule can interact, with changes in the conformation of a membrane-associated protein altering either its catalytic activity or the protein's interaction with other membrane proteins. Mammalian dietary studies attempted to change the lipid composition of a few cell membranes have shown comparisons, using essential fatty acid-deficient diets. In recent years, Clandinin and a few other workers have pioneered the study proving the influence of dietary fat fed in a nutritionally complete diet on composition of phospholipid classes of cell membrane. Modulation caused by diet fat was rapid and reversible in phospholipid fatty acyl composition of membranes of cardiac mitochondria, liver cell, brain synaptosome and lymphocytes. These changes were at the same time, accompanied by variety of membrane associated functions controlled by membrane-bound enzymes, tranporter and receptor proteins. The findings suggest the basic concept of the necessity of dietary fatty acid balance if consistency of optimal membrane structural lipid composition is to be maintained, as well as the overall inadequacy of describing the nutritional-biochemical quality of a dietary fat solely by its content of linoleic acid. Furthermore, they give light on the possible application to clinical and preventive medicine.

• KSBB Journal
• /
• 제20권4호
• /
• pp.305-310
• /
• 2005

동결건조 양막과 자체 제작한 양막 지지체를 이용한 동적 배양법을 통하여 토끼 각막 상피층의 재구성 효과를 검토하였다. 각막 상피세포는 토끼 각막 윤부로부터 분리 배양 후 동결보존하여 사용하였고, 세포증식은 10계대까지 가능하였다. 동적배양을 한 경우가 정치배양보다 기저층이 잘 형성되었고, 세포 증식과 분화를 촉진하여 치밀한 상피층을 형성하였다. 이렇게 재구성된 각막 상피층은 심한 각막 손상을 입은 환자의 조직 이식에 좋은 생체외 모델이 될 것으로 기대된다.

• Membrane and Water Treatment
• /
• 제14권3호
• /
• pp.115-120
• /
• 2023

The design theory for nanoporous filtration membranes needs to be established. The present study shows that the performance and technical advancement of nanoporous filtration membranes are determined by the fundamental parameter I (in the unit Watt^1/2) which is formulated as a function of the shear strength of the liquid-pore wall interface, the radius of the filtration pore, the membrane thickness, and the bulk dynamic viscosity of the flowing liquid. This parameter determines the critical power loss on a single filtration pore for initiating the wall slippage, which is important for the flux of the membrane. It also relates the membrane permeability to the power cost by the filtration pore. It is shown that for biological cellular membranes its values are on the scale 1.0E-8Watt^1/2, for mono-layer graphene membranes its values are on the scale 1.0E-9Watt^1/2, and for nanoporous membranes made of silica, silicon nitride or silicon carbonized its values are on the scale 1.0E-5Watt^1/2. The scale of the value of this parameter directly measures the level of the performance of a nanoporous filtration membrane. The carbon nanotube membrane has the similar performance with biological cellular membranes, as it also has the value of I on the scale 1.0E-8Watt^1/2.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 1997년도 추계학술대회논문집; 한국과학기술회관; 6 Nov. 1997
• /
• pp.396-401
• /
• 1997

Dynamic stability of cylindrical shells subjected to follower forces is analyzed in this paper. Motion of shells is formulated in curvilinear coordinates that is consistent with assumptions made in the Timoshenko beam and the Mindlin plate. Using the finite element method, the induced equations are reduced to an equation with finite degrees of freedom. The 9-node Lagrangian element is used, and reduced integration is used to avoid shear and membrane locking. The effects of thickness ratio on the dynamic stability of cylindrical shells are studied.