KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Reconstruction of Rabbit Corneal Epithelium using Lyophilized Amniotic Membrane and Dynamic Culture Method

동결건조 양막과 동적배양법을 이용한 토끼 각막 상피층의 재구성

  • Ahn, Jae-Il (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Jang, In-Keun (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Ophthalmology, Chung-Ang University Hospital) ;
  • Shin, Youn-Ho (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Seo, Young-Kwon (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Ophthalmology, Chung-Ang University Hospital) ;
  • Yoon, Hee-Hun (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Yoon, Mun-Young (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Kim, Jae-Chang (Department of Ophthalmology, Chung-Ang University Hospital) ;
  • Song, Kye-Yong (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Lee, Hee-Gu (Molecular and Cell Biology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yang, Eun-Kyung (R&D Center, Bioland Ltd.) ;
  • Kim, Ki-Ho (R&D Center, Bioland Ltd.) ;
  • Park, Jung-Keug (Department of Chemical and Biochemical Engineering, Dongguk University)
  • 안재일 (동국대학교 생명화학공학과) ;
  • 장인근 (동국대학교 생명화학공학과, 중앙대학교 부속 용산병원 안과) ;
  • 신연호 (동국대학교 생명화학공학과) ;
  • 서영권 (동국대학교 생명화학공학과) ;
  • 윤희훈 (동국대학교 생명화학공학과) ;
  • 윤문영 (동국대학교 생명화학공학과) ;
  • 김재찬 (중앙대학교 부속 용산병원 안과) ;
  • 송계용 (중앙대학교 의과대학 병리학교실) ;
  • 이희구 (한국생명공학연구원 세포생물학연구실) ;
  • 양은경 (바이오랜드 생명공학연구소) ;
  • 김기호 (바이오랜드 생명공학연구소) ;
  • 박정극 (동국대학교 생명화학공학과)
  • Published : 2005.08.01
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Abstract

Reconstruction of rabbit corneal epithelium was performed through dynamic culture method using self-manufactured amniotic membrane supporter and lyophilized amniotic membrane. Rabbit corneal epithelial cells were cultured and cryopreserved after isolation from limbus, and the cells could be proliferated by passage number 10. The basal layer was well formed, and the epithelium layer was constructed tightly by the increase of cell proliferation and differentiation by dynamic culture method than static culture. Thus, the reconstruction of the corneal epithelium using lyophilized amniotic membrane is considered to be a good in vitro model for transplantation of corneal epithelium to patients with a severely damaged cornea.

동결건조 양막과 자체 제작한 양막 지지체를 이용한 동적 배양법을 통하여 토끼 각막 상피층의 재구성 효과를 검토하였다. 각막 상피세포는 토끼 각막 윤부로부터 분리 배양 후 동결보존하여 사용하였고, 세포증식은 10계대까지 가능하였다. 동적배양을 한 경우가 정치배양보다 기저층이 잘 형성되었고, 세포 증식과 분화를 촉진하여 치밀한 상피층을 형성하였다. 이렇게 재구성된 각막 상피층은 심한 각막 손상을 입은 환자의 조직 이식에 좋은 생체외 모델이 될 것으로 기대된다.

Keywords

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