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Reconstruction of Rabbit Corneal Epithelium using Lyophilized Amniotic Membrane and Dynamic Culture Method  

Ahn, Jae-Il (Department of Chemical and Biochemical Engineering, Dongguk University)
Jang, In-Keun (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Ophthalmology, Chung-Ang University Hospital)
Shin, Youn-Ho (Department of Chemical and Biochemical Engineering, Dongguk University)
Seo, Young-Kwon (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Ophthalmology, Chung-Ang University Hospital)
Yoon, Hee-Hun (Department of Chemical and Biochemical Engineering, Dongguk University)
Yoon, Mun-Young (Department of Chemical and Biochemical Engineering, Dongguk University)
Kim, Jae-Chang (Department of Ophthalmology, Chung-Ang University Hospital)
Song, Kye-Yong (Department of Pathology, College of Medicine, Chung-Ang University)
Lee, Hee-Gu (Molecular and Cell Biology Research Division, Korea Research Institute of Bioscience and Biotechnology)
Yang, Eun-Kyung (R&D Center, Bioland Ltd.)
Kim, Ki-Ho (R&D Center, Bioland Ltd.)
Park, Jung-Keug (Department of Chemical and Biochemical Engineering, Dongguk University)
Publication Information
KSBB Journal / v.20, no.4, 2005 , pp. 305-310 More about this Journal
Abstract
Reconstruction of rabbit corneal epithelium was performed through dynamic culture method using self-manufactured amniotic membrane supporter and lyophilized amniotic membrane. Rabbit corneal epithelial cells were cultured and cryopreserved after isolation from limbus, and the cells could be proliferated by passage number 10. The basal layer was well formed, and the epithelium layer was constructed tightly by the increase of cell proliferation and differentiation by dynamic culture method than static culture. Thus, the reconstruction of the corneal epithelium using lyophilized amniotic membrane is considered to be a good in vitro model for transplantation of corneal epithelium to patients with a severely damaged cornea.
Keywords
Lyophilized amniotic membrane; reconstruction of corneal epithelium; static culture; dynamic culture;
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