• Reproductive and Developmental Biology
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• v.28 no.1
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• pp.7-12
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• 2004

This study was performed to establish the effective culture condition for the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were cultured and passaged two times before use. A single cell was seeded in 96-well plates, cultured in medium supplemented with different concentrations of FBS, catalase or $\beta$-mercaptoethanol ($\beta$ME), and classified by cell size and morphology. Cells were passaged two times into 4-well dish before freezing. The establishment efficiencies were not different among different concentrations of FBS (0.3 to 5.1%). However, population doubling time (PDT) was significantly decreased by increasing the FBS concentration (P＜0.05). The establishment efficiency of $\beta$ME-added group (10.4%) was significantly higher than those of catalase-added and control groups (3.5%, and 3.5%, respectively, p＜0.05), and PDT was significantly decreased (23.6 vs 28.1, and 25.5 h, respectively, p＜0.05). However, catalase did not show a positive effect on the establishment efficiency. Cell size and morphology did not affect the establishment efficiency and PDT of clonal lines. The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 30% FBS and $\beta$ME.

• The Korean Journal of Zoology
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• v.34 no.2
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• pp.148-158
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• 1991

The purpose of this research was to establish the culture condition for dissociated submandibu -lar gland (SG) cells. After trypsin digestion of SG from 3-4 weeks old mice, dissociated cells were cultured in 1OO/o fetal bovine serum-Dulbecco's modified Eagle's medium (FBS-DME) or 0.5-2% low protein serum replacement-DME (LPSR-DME) on plastic surface to form monolayer. The effects of FBS, LPSR and hormones on the growth and function of cultured SG cells were examined. SG cells dissociated by enzyme were successfully cultured and were characterized as epithelial-like cells by light and electron microscope. The maximal DNA synthesis of cultured SG cells was achieved by DME containing 5-10% FBS. The same results were obtained when the effects of LPSR on cell proliferation were examined up to a LPSR concentration of 2%. SG cells cultured in 20/o LPSR-DME expressed a population doubling time of 42.5 hrs and a saturation density of 1.2 $\times$10 5cell/cm$^2$. Dihydrotestosterone (DHT) in medium did not influence on the DNA synthesis of the cultured SG cells, but stimulated protein synthesis of the SG cells. Thyroxine (T4) stimulated protein synthesis of the SCI cells markedly in a dose-dependent fashion. EGF secretion by the cultured SG cells increased significandy by DHT and or T4 trearment. This finding indicated that secretion of EGF by the SG cells was under the control of the hormones such as androgen and thyroid hormones. It seems to be that the culture condition described here can be used as a useful tool for further research on the SCI cells.

• Proceedings of the IEEK Conference
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• 2003.07c
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• pp.2835-2838
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• 2003

We study the characteristics of oscillating in non-autonomous condition and the conducted noise generation in a RL-photocoupler circuit. This circuit may be shown a period-doubling and a chaos dynamics under any specific conditions of input circuit. But, the relationship between input signals and output signals is different according to the amplitude of driving input voltage. Then, the oscillation noise was analyzed with respect to both the frequency and the amplitude of an external ac signal and do values. The results show that the noise-induced oscillations for falling and rising cycles induced by kick-back effect in an inductor, nonlinear capacitance, nonlinear resistance and charge storage time in a diode and an LED. We also compared the simulation with the experimental results.

• Bulletin of the Korean Mathematical Society
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• v.36 no.1
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• pp.25-31
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• 1999

Let $\nu$ be a positive Borel measure on a space of homogeneous type (X, d, $\mu$), satisfying the doubling property. A condition on a weight $\omega$ for whixh a maximal operator $M\nu f$(x) defined by M$mu$f(x)=supr>0{{{{ { 1} over {ν(B(x,r)) } INT _{ B(x,r)} │f(y)│d mu (y)}}}}, is of weak type (p,p) with respect to (ν, $omega$), is that there exists a constant C such that C $omega$(y) for a.e. y$\in$B(x, r) if p=1, and {{{{( { 1} over { upsilon (B(x,r) } INT _{ B(x,r)}omega(y) ^ (-1/p-1) d mu (y))^(p-1)}}}} C, if 1$infty$.

• Proceedings of the KIPE Conference
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• 1999.07a
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• pp.650-654
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• 1999

In this paper, author describe the simulation results concerning the period doubling bifurcation route to chaos of DC/DC boost converter under current mode control to show that it is common phenomena on switching regulator when parameters are improperly chosen or continuously varied beyond the ensured region by system designer. Bifurcation diagrams of periodic orbits of inductor current and capacitor voltage of DC/DC boost converter are plotted with sampled data at moment of each clock pulse causing switching on. DC/DC boost converter studied on this paper is modelled by its state space equations as per switching condition under continuous conduction mode. Current reference signal and capacitance are chosen as the bifurcation parameters and those are varied in step for iterative calculation to find bifurcation points of periodic orbits of state variables.

• Journal of Biomedical Engineering Research
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• v.17 no.3
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• pp.355-364
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• 1996

Construction of the stable monolayer of endothelial cells onto physicochemically modified polymeric surFace is one of the appropriate method to develop the small caliber vascular graft with the long-term patency. In this study, we constructed the monolayer of endothelial cells on the fibronectin rind the extracellular matrix-coated polyurethane surface derived from human fibroblast cells. To elucidate the adhesion strength of endothelial cells on the extracellular matrix-coated polyurethane, a laminar flow chamber apparatus was developed to exposure the shear stress on the apical membrane of ondothelial cells. Endothelial cells show the strongest adhesion after two days of seeding onto the fibronectin-coated polyurethane surface, whereas endothelial cells on the extracellular matrix derived from the human flbroblast cells show the minimal doubling time of cellular growth.

• Journal of Family Resource Management and Policy Review
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• v.10 no.4
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• pp.125-143
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• 2006

The purpose of this study is to develop and test the effectiveness of a financial education program that make the elderly recognize financial problems and find available strategy to improve their financial situation. Based on the literature review and economic structure of the elderly, a financial education program was developed with three parts: 'My elderly lift'; 'Doubling my income'; and 'Safe investment strategy.' 42 elderly people in Senior academy participate in three-session program and 39 people completed pre and post-test and satisfaction survey. In order to examine the effectiveness of the program paired t-test was used. According to evaluation results, the program was found to have a positive effect on enhancing knowledge in elderly lift and financial management, but not on changing attitude. Most of participants show high level of satisfaction with the program.

• Nuclear Engineering and Technology
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• v.32 no.3
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• pp.274-282
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• 2000

The simulation of water balance was conducted for suggested four alternative multi-layer cover design of near-surface radioactive waste disposal facility under domestic climate condition. The analysis was also conducted for the most favorable one out of four alternative cover design under conservative scenarios. Until 100 years after closure of disposal vault, the infiltration flux for the most favorable cover design was negligible even under doubling of the ambient precipitation condition. When the degradation of asphalt and geomembrane after 100 years of closure was considered, the infiltration flux significantly increased almost to the design criteria of cover system in I' Aube disposal facility. And it was found that the hydraulic conductivity of bentonite/sand as a bottom barrier should be no greater than 1$\times$10$^{-7}$ cm/sec recommended by U.S. EPA.

• Fashion & Textile Research Journal
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• v.5 no.4
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• pp.408-412
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• 2003

The micro structure of POY was modified and a wool-like touch yarn of composite fibers with different shrinkage was made. With this yarn 12 different fabrics with wool like touch were prepared. The characteristic physical property changes of the fabrics examined are as follows: 1. In all cases, the initial high shrinkage stages were observed in hot water treatment and the 3D images of complex multilayer of typical doubling fibers with different shrinkage were also observed in hot air treatment of 170C. 2. The tensile strength changes of satin and plain fabrics with the change of twist count showed similar behavior. However, WT's were slightly higher and RT's was lower in twill and satin fabrics than those in plain fabric. 3. Since a slight decrease of B's of twill fabric found with increasing twist count under given experimental condition, it could be influenced on the anti-drape stiffness was decreased and flexibility was increased. 4. A significant decrease of G values was observed in the twist count 800-1000 T.P.M However, in the twist count higher than 1000 T.P.M G values observed were kept nearly constant. 5. MIU of plain and twill fabrics showed a drastic decrease at the twist count higher than 1000 T.P.M.

• Fashion & Textile Research Journal
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• v.9 no.3
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• pp.347-350
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• 2007

To analyse basic properties for making packing batt according to doubling condition, packing batt yarn, of $300^D$, $900^D$, $3600^D$ made from DTY yarn $150^D$/48 were produced from KTDI. The results are as follows: The birefringence of the sample yarn increased with increasing the annealing temperature and denier. The initial modulus of the sample yarn decreased with increasing the annealing temperature and denier. The higher than annealing temperature of $160^{\circ}C$, initial modulus of the sample are equilibrated. The strain recovery ratio of samples decreased with increasing the annealing temperature and denier. The lower than annealing temperature of $140^{\circ}C$, strain recovery ratio of the sample are decreased Where the $900^D$, $3600^D$ yarns are at $100^{\circ}C$ the specific bending rigidity value obtained is 0.65kgf/d but the twisted yarn (3,600) obtained 0.006 ($gfcm^2/tex^2$). However, where the heat temperature is $160^{\circ}C$, specific bending rigidity value obtained 0.003($gfcm^2/tex^2$).