• 제목/요약/키워드: double sequence

검색결과 306건 처리시간 0.023초

Genome Analysis of Phage SMSAP5 as Candidate of Biocontrol for Staphylococcus aureus

  • Lee, Young-Duck;Park, Jong-Hyun
    • 한국축산식품학회지
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    • 제35권1호
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    • pp.86-90
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    • 2015
  • In this study, we reported the morphogenetic analysis and genome sequence by genomic analysis of the newly isolated staphylococcal phage SMSAP5 from soil of slaughterhouses for cattle. Based on transmission electron microscopy evident morphology, phage SMSAP5 belonged to the Siphoviridae family. Phage SMSAP5 had a double-stranded DNA genome with a length of 45,552 bp and 33 % G+C content. Bioinformatics analysis of the phage genome revealed 43 open reading frames. A blastn search revealed that its nucleotide sequence shared a high degree of similarity with that of the Staphylococcus phage tp310-2. In conclusion, this study is the first report to show the morphological features and the complete genome sequence of the phage SMSAP5 from soil of slaughterhouses for cattle.

Genomic analysis of WCP30 Phage of Weissella cibaria for Dairy Fermented Foods

  • Lee, Young-Duck;Park, Jong-Hyun
    • 한국축산식품학회지
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    • 제37권6호
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    • pp.884-888
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    • 2017
  • In this study, we report the morphogenetic analysis and genome sequence of a new WCP30 phage of Weissella cibaria, isolated from a fermented food. Based on its morphology, as observed by transmission electron microscopy, WCP30 phage belongs to the family Siphoviridae. Genomic analysis of WCP30 phage showed that it had a 33,697-bp double-stranded DNA genome with 41.2% G+C content. Bioinformatics analysis of the genome revealed 35 open reading frames. A BLASTN search showed that WCP30 phage had low sequence similarity compared to other phages infecting lactic acid bacteria. This is the first report of the morphological features and complete genome sequence of WCP30 phage, which may be useful for controlling the fermentation of dairy foods.

SSFPI 기법을 이용한 MR 뇌기능 영상 -고 속의 자화율 효과의 직접적인 측정 (SSFP Interferometry (SSFPI) Technique Applied to functional MRI - A Fast and Direct Measurement of Magnetic Susceptibility Effect)

  • 정준영
    • 대한의용생체공학회:의공학회지
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    • 제17권4호
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    • pp.525-534
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    • 1996
  • We have developed a fast steady state free precession interferometry (SSFPI) technique which is useful for the fMRl (functional Magnetic Resonance Imaging). As is known, SSFP sequence with a suitable adjustment of Vadient (readeut) allows us to measure precession angle 6 which in tw relates to the field inhomogeneity. Combining the two pulses (known as FID and Echo) in FADE (Fast Acquisition Double Echo) sequence, for example, one can obtain the interference term which is directly related to the precession angle It has been known that a fast high resolution magnetic field mapping is possible by use of the modified FADE sequence or SSFPI, and we have attempted to use the SSFPI technique for the susceptibility-induced fMRl. When the method is applied to the susceptibility effect based functional magnetic resonance imaging (fMRl), it was found that the direct susceptibility effect measurement was possible without perturbations such as the backgrounds and inflow effect. In this paper, simulation results and experimental results obtained with 2.0 Tesla MRI system are presented.

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NMR Study on Thermal Stability of the Double Helical Structures of d(CGAATTCG)2, d(CGTATACG)2 and their berenil complexes

  • Kim, Eun-Hee;Hong, Seok-Joo;Huh, Sung-Ho
    • 한국자기공명학회논문지
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    • 제8권2호
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    • pp.96-107
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    • 2004
  • We prepared two oligonucleotides containing same base pairing, but different base sequence in the middle region, d(CGAATTCG) and d(CGTATACG). NMR and UV absorbance data represented that such variation in base sequence could cause a significant difference in melting temperature and dynamics between d(CGAATTCG)$_2$ and d(CGTATACG)$_2$ duplexes, which are regarded to be associated with the stacked structure and the width of the minor groove of them. The latter showed poor stability compared to the former, because of poor stacking of bases. And berenil could bind to the minor groove of d(CGAATTCG)$_2$ which is relatively narrow, more strongly than d(CGTATACG)$_2$ and this gave rise to large improvement in thermal stability of the d(CGAATTCG)$_2$ duplex, compared to d(CGTATACG)$_2$.

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Numerical study of bonded composite patch repair in damaged laminate composites

  • Azzeddine, Nacira;Benkheira, Ameur;Fekih, Sidi Mohamed;Belhouari, Mohamed
    • Advances in aircraft and spacecraft science
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    • 제7권2호
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    • pp.151-168
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    • 2020
  • The present study deals with the repair of composite structures by bonding composite patches. The composite structure is a carbon/epoxy laminate with stacking sequence [45/-45/0/90]S. The damaged zone is simulated by a central crack and repaired by bonding symmetrical composite patches. The repair is carried out using composite patches laminated from the same elemental folds as those of the cracked specimen. Three-dimensional finite element method is used to determine the energy release rate along the front of repaired crack. The effects of the repair technique used single or double patch, the stacking sequence of the cracked composite patch and the adhesive properties were highlighted on the variations of the fracture energy in mode I and mixed mode I + II loading.

탄소섬유/에폭시 복합재료의 층간파괴인성에 미치는 균열진전각도의 영향 (Effect of Crack Propagation Directions on the Interlaminar Fracture Toughness of Carbon/Epoxy Composite Materials)

  • 황진호;황운봉
    • 대한기계학회논문집A
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    • 제23권6호
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    • pp.1026-1038
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    • 1999
  • Interlaminar fracture toughness of carbon/epoxy composite materials has been studied under tensile and flexural loading by the use of width tapered double cantilever beam(WTDCB) and end notched flexure(ENF) specimens. This study has significantly examined the effect of various interfacial ply orientation, ${\alpha}(0^{\circ},\;45^{\circ}\;and\;90^{\circ})$ and crack propagation direction, ${\theta}(0^{\circ},\;15^{\circ},\;30^{\circ}\;and\;45^{\circ})$ in terms of critical strain energy release rate through experiments. Twelve differently layered laminates were investigated. The data reduction for evaluating the fracture energy is based on compliance method and beam theory. Beam theory is used to analyze the effect of crack propagation direction. The geometry and lay-up sequence of specimens are considered various conditions such as skewness parameter, beam volume, and so on. The results show that the fiber bridging occurred due to the non-midplane crack propagation and causes the difference of fracture energy evaluated by both methods. For safer and more reliable composite structures, we obtain the optimal stacking sequence from initial fracture energy in each mode.

Characteristic of Induction Motor Drives Fed by Three Leg and Five Leg Inverters

  • Talib, Md. Hairul Nizam;Ibrahim, Zulkifilie;Rahim, Nasrudin Abd.;Hasim, Ahmad Shukri Abu
    • Journal of Power Electronics
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    • 제13권5호
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    • pp.806-813
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    • 2013
  • This paper aims to compare the performance of three phase induction motor drives using Five Leg Inverter (FLI) and Three Leg Inverter (TLI) configurations. An Indirect Field Oriented Control (IFOC) method using a TLI is well established and incorporated for high performance speed drives in various industries. The FLI dual motor drive system on the other hand shows good workability in the independent control of two induction motor drives simultaneously. In this experiment, the IFOC method is utilized for both drive systems, and Space Vector Pulse Width Modulation (SVPWM) is used to generate pulses for both inverters. For the FLI, the Double Zero Sequence (DZS) Injection technique is used to generate the modulation signal. The complete experiment setup is done by using a DSpace 1103 controller board. The individual motor performances are analyzed using similar schemes, equipment setups and controller parameter values. The results show similar speed performance response capability between the single motor operation using a TLI system and the two motor operation using a FLI system based on the variable speed range either in forward or reverse operation. They also show similar load rejection abilities. However, the single motor with a TLI has a better power quality aspect such as ripple current and total harmonics distortion (THD).

자기단 전원 임피던스 추정 기법을 사용한 병행 2회선 송전선로 고장점 표정 알고리즘 (A Fault Location Algorithm Using Adaptively Estimated Local Source Impedance for a Double-Circuit Transmission Line System)

  • 박건호;강상희;김석일;신종한
    • 전기학회논문지
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    • 제61권3호
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    • pp.373-379
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    • 2012
  • This paper presents a fault location algorithm based on the adaptively estimated value of the local sequence source impedance for faults on a parallel transmission line. This algorithm uses only the local voltage and current signals of a faulted circuit. The remote current signals and the zero-sequence current of the healthy adjacent circuit are calculated by using the current distribution factors together with the local terminal currents of the faulted circuit. The current distribution factors consist of local equivalent source impedance and the others such as fault distance, line impedance and remote equivalent source impedance. It means that the values of the current distribution factors can change according to the operation condition of a power system. Consequently, the accuracy of the fault location algorithm is affected by the two values of equivalent source impedances, one is local source impedance and the other is remote source impedance. Nevertheless, only the local equivalent impedance can be estimated in this paper. A series of test results using EMTP simulation data show the effectiveness of the proposed algorithm. The proposed algorithm is valid for a double-circuit transmission line system where the equivalent source impedance changes continuously.

Change of transmission characteristics of FSSs in hybrid composites due to residual stresses

  • Hwang, In-Han;Chun, Heoung-Jae;Hong, Ik-Pyo;Park, Yong-Bae;Kim, Yoon-Jae
    • Steel and Composite Structures
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    • 제19권6호
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    • pp.1501-1510
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    • 2015
  • The frequency selective surface (FSS) embedded hybrid composite materials have been developed to provide excellent mechanical and specific electromagnetic properties. Radar absorbing structures (RASs) are an example material that provides both radar absorbing properties and structural characteristics. The absorbing efficiency of an RAS can be improved using selected materials having special absorptive properties and structural characteristics and can be in the form of multi-layers or have a certain stacking sequence. However, residual stresses occur in FSS embedded composite structures after co-curing due to a mismatch between the coefficients of thermal expansion of the FSS and the composite material. In this study, to develop an RAS, the thermal residual stresses of FSS embedded composite structures were analyzed using finite element analysis, considering the effect of stacking sequence of composite laminates with square loop (SL) and double square loop (DSL) FSS patterns. The FSS radar absorbing efficiency was measured in the K-band frequency range of 21.6 GHz. Residual stress leads to a change in the deformation of the FSS pattern. Using these results, the effect of transmission characteristics with respect to the deformation on FSS pattern was analyzed using an FSS Simulator.

ITO/AZO 투명전극을 이용한 Si 기반의 광전소자 (Si Based Photoelectric Device with ITO/AZO Double Layer)

  • 장희준;윤한준;이경남;김준동
    • 한국전기전자재료학회논문지
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    • 제31권2호
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    • pp.85-89
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    • 2018
  • In this study, functional transparent conducting layers were investigated for Si-based photoelectric applications. Double transparent conductive oxide (TCO) films were deposited on a Si substrate in the sequence of indium tin oxide (ITO) followed by aluminum-doped zinc oxide (AZO). First, we observed that the conductivity and transparency of AZO dominate the overall performance of the double TCO layers. Secondly, the double layered TCO film (consisting of AZO/ITO) deposited by sputtering was compared to a AZO-only film in terms of their optical and electrical properties. We prepared three different AZO films: ITO:3min/AZO:10min, ITO:5min/AZO:7min, and ITO:7min/AZO:4min. The results show that the optical properties (transmittance, absorbance, and reflection) can be controlled by the film composition. This may provide a significant pathway for the manipulation of the optical and electrical properties of photoelectric devices.