• Bulletin of the Society of Naval Architects of Korea
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• v.26 no.2
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• pp.25-31
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• 1989

Two-dimensional large-amplitude motions in regular harmonic wave are treated in time domain, by satisfying the exact body boundary condition and the linear free surface condition. For the present numerical calculation, the method of free-surface spectral representation with simple source distribution on the instantaneous body surface has been extended to include the effect of the incident wave. Calculations of the wave exciting force are performed for a submerged circular cylinder fixed or oscillating with large amplitude. Especially, nonlinear effects on the time-mean forces are studied in detail. It is shown that relative motion between the body and the fluid particle gives a significant effect on the lift and drift forces. Also, large-amplitude motion of a submerged circular cylinder and that of a floating Lewis-form cylinder are directly simulated in time domain. In the calculation results, some nonlinear effects are shown.

• Toxicological Research
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• v.28 no.1
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• pp.25-31
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• 2012

The purpose of this study was to determine the acute pulmonary toxicity of metallic silver nanoparticles (MSNPs, 20.30 nm in diameter). Acute pulmonary toxicity and body distribution of inhaled MSNPs in mice were evaluated using a nose-only exposure chamber (NOEC) system. Bronchoalveolar lavage (BAL) fluid analysis, Western blotting, histopathological changes, and silver burdens in various organs were determined in mice. Mice were exposed to MSNPs for 6 hrs. The mean concentration, total surface area, volume and mass concentrations in the NOEC were maintained at $1.93{\times}10^7$ particles/$cm^3$, $1.09{\times}10^{10}\;nm^2/cm^3$, $2.72{\times}10^{11}\;nm^3/cm^3$, and 2854.62 ${\mu}g/m^3$, respectively. Inhalation of MSPNs caused mild pulmonary toxicity with distribution of silver in various organs but the silver burdens decreased rapidly at 24-hrs post-exposure in the lung. Furthermore, inhaled MSNPs induced activation of mitogen-activated protein kinase (MAPK) signaling in the lung. In summary, single inhaled MSNPs caused mild pulmonary toxicity, which was associated with activated MAPK signaling. Taken together, our results suggest that the inhalation toxicity of MSNPs should be carefully considered at the molecular level.

• The Journal of the Korean Society for Microbiology
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• v.18 no.1
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• pp.53-58
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• 1983

Enterotoxigenic E. coli is one of causative agents of the infantile diarrhea and traveler's diarrhea. A modified infant mouse assay(IMA) was developed for the detection of heat stable enterotoxin (ST) of E. coli isolated from diarrheal and control infants and assay system was established with using enterotoxin producing reference strains. The supernatant of the 24 hour-shaking culture of E. coli in Casamino Acid Yeast Extract Salt Broth(CYES-2) was ingested orally into the 2-4 day old ICR mice. After the mice were kept at $25^{\circ}C$ for 4 hours, they were sacrificed and the gut weight body weight ratio(GW/BW) was taken as the index of fluid accumulation induced by heat stable enterotoxin of E. coli. The results obtained were as follows; 1. The GW/BW responses of IMA tested with enterotoxin reference strains of E. coli(E. coli O148H28:$ST^+LT^+$, E. coli $O78H^-:ST^+LT^+$, E. coli O15H11:$ST^-LT^+$, E. coli O1H7:$ST^-LT^-$) appeared ta be ST dose-dependent, and not LT-dependent. From the dose-response curve, $25{\mu}l$ of culture supernatant was determined as test amount of the IMA. 2. Frequency distribution of IMA result from 643 strain of E. coli showed normal distribution at low GW/BW ratio and dispersed pattern at high GW/BW ratio. The GW/BW ratios of $0.056{\pm}0.004(mean{\pm}SD)$ of normal distribution which distributed from 0.044 to 0.068(P<0.01) was considered as ST negative. Thus the GW/BW ratio above 0.069 could be regarded as ST positive.

• Environmental Analysis Health and Toxicology
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• v.25 no.3
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• pp.215-222
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• 2010

The market size of engineered nanoparticles is rapidly increasing due to the fast application of nanotechnologies into different industries and consumer products. The development of new technology and materials has improved human's quality of life, but it also entails the possibility of exposure to new materials. In this study, we compared the distribution in the body by the inflow of silver nanoparticles having another diameter and shape at 1 h or 24 h after injection via the tail vein. And, we compared the cell composition and cytokine concentration in BAL fluid, and histopathological changes. As results, discharge of silver nanoparticles having small diameter and sphere shape was more rapid than that of big diameter or plate shape. It is estimated that the toxicity in liver and lung was proportional to accumulation level. The persistence of inflammation was also longer in mice treated with plate shape. Consequently, we suggest that the first choice of silver nanoparticles having small diameter and sphere shape in applying is desirable.

• Toxicological Research
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• v.30 no.1
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• pp.55-63
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• 2014

Objectives: The use of indium compounds, especially those of small size, for the production of semiconductors, liquid-crystal panels, etc., has increased recently. However, the role of particle size or the chemical composition of indium compounds in their toxicity and distribution in the body has not been sufficiently investigated. Therefore, the aim of this study was to examine the effects of particle size and the chemical composition of indium compounds on their toxicity and distribution. Methods: Male Sprague-Dawley rats were exposed to two different-sized indium oxides (average particle sizes under 4,000 nm [IO_4000] and 100 nm [IO_100]) and one nano-sized indium-tin oxide (ITO; average particle size less than 50 nm) by inhalation for 6 hr daily, 5 days per week, for 4 weeks at approximately $1mg/m^3$ of indium by mass concentration. Results: We observed differences in lung weights and histopathological findings, differential cell counts, and cell damage indicators in the bronchoalveolar lavage fluid between the normal control group and IO- or ITO-exposed groups. However, only ITO affected respiratory functions in exposed rats. Overall, the toxicity of ITO was much higher than that of IOs; the toxicity of IO_4000 was higher than that of IO_100. A 4-week recovery period was not sufficient to alleviate the toxic effects of IO and ITO exposure. Inhaled indium was mainly deposited in the lungs. ITO in the lungs was removed more slowly than IOs; IO_4000 was removed faster than IO_100. IOs were not distributed to other organs (i.e., the brain, liver, and spleen), whereas ITO was. Concentrations of indium in the blood and organ tissues were higher at 4 weeks after exposure. Conclusions: The effect of particle size on the toxicity of indium compounds was not clear, whereas chemical composition clearly affected toxicity; ITO showed much higher toxicity than that of IO.

• Tribology and Lubricants
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• v.32 no.2
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• pp.50-55
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• 2016

Recently, a double-side machining process has been adopted in fabricating a sapphire glass to enhance the manufacturability. Double-side lap grinding (DLG) is one of the emerging processes that can reduce process steps in the fabrication of sapphire glasses. The DLG process uses two-body abrasion with fixed abrasives including pallet. This process is designed to have a low pressure and high rotational speed in order to obtain the required material removal rate. Thus, the temperature is distributed on the DLG platen during the process. This distribution affects the shape of the substrate after the DLG process. The coolant that is supplied into the cooling channel carved in the base platen can help to control the temperature distribution of the DLG platen. This paper presents the results of computational fluid dynamics with regard to the heat transfer in a DLG platen, which can be used for fabricating a sapphire glass. The simulation conditions were 200 rpm of rotational speed, 50℃ of frictional temperature on the pallet, and 20℃ of coolant temperature. The five cases of the coolant flow rate (20~36 l/min) were simulated with a tetrahedral mesh and prism mesh. The simulation results show that the capacity of the generated cooling system can be used for newly developed DLG machines. Moreover, the simulation results may provide a process parameter influencing the uniformity of the sapphire glass in the DLG process.

• Wind and Structures
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• v.23 no.5
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• pp.465-483
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• 2016

This study aims to enhance the understanding of the surface pressure distribution around rectangular bodies, by considering aspects such as the suction pressure at the leading edge on the top and side faces when the body aspect ratio and wind direction are changed. We carried out wind tunnel measurements and numerical simulations of flow around a series of rectangular bodies (a cube and two rectangular bodies) that were placed in a deep turbulent boundary layer. Based on a modern numerical platform, the Navier-Stokes equations with the typical two-equation model (i.e., the standard $k-{\varepsilon}$ model) were solved, and the results were compared with the wind tunnel measurement data. Regarding the turbulence model, the results of the $k-{\varepsilon}$ model are in overall agreement with the experimental results, including the existing data. However, because of the blockage effects in the computational domain, the pressure recovery region is underpredicted compared to the experimental data. In addition, the $k-{\varepsilon}$ model sometimes will fail to capture the exact flow features. The primary emphasis in this study is on the flow characteristics around rectangular bodies with various aspect ratios and approaching wind directions. The aspect ratio and wind direction influence the type of wake that is generated and ultimately the structural loading and pressure, and in particular, the structural excitation. The results show that the surface pressure variation is highly dependent upon the approaching wind direction, especially on the top and side faces of the cube. In addition, the transverse width has a substantial effect on the variations in surface pressure around the bodies, while the longitudinal length has less influence compared to the transverse width.

• Bulletin of the Society of Naval Architects of Korea
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• v.22 no.3
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• pp.9-18
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• 1985

The numerical calculation for solving boundary-value problem related to potential flows with a free surface is carried out by application of the localized finite element method. Only forced motion of 2-D body in infinitely deep fluid is considered, although this schemes is equally applicable to any first order time-harmonic problems of similar nature. The infinite domain of the fluid is separated into the inner flow field and the outer flow field with common inter-surface boundary. The finite element method is applied to obtain the solution in the inner flow field and the Green functions are utilized to represent the solution in the outer flow field. At the inter-surface boundary, the continuity of the value of potential and the normal derivative of the potential(i.e. matching condition) is conserved. The present method has better computational efficiency than the previous LFEM and the integral equation method of Frank. This enhanced computational efficiency is presumably due to the fact that the present method gives a symmetric coefficient matrix and requires less computational time in calculating the influence coefficient matrix of Green function than the integral equation method. And the irregular frequency desen't exist because the uniqueness of the solution is assured by the such that the exact free surface condition is satisfied on the boundary of the localized finite element region(i.e. inner region). As an example of the above method, the hydrodynamic forces for the circular cylinder and the rectangular cylinders are calculated. In the computed results, the small number of singularity distribution segments($3{\sim}6$) give good result relative to Ursell's and Vugts'.

• Journal of Animal Science and Technology
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• v.63 no.6
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• pp.1433-1442
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• 2021

In this study, Saccharomyces cerevisiae culture fluid (SCCF) has been added to a diet of lactating dairy cows to attempt to improve the ruminal fermentation and potentially increase the dry matter intake (DMI) and milk yield. This study was conducted to investigate the effects of SCCF on the milk yield and blood biochemistry in lactating cows during the summer. Twenty-four Holstein dairy cows were randomly assigned to one of four treatments: (1) total mixed ration (TMR-1) (Control); (2) TMR-1 supplemented with SCCF (T1); (3) TMR-2 (containing alfalfa hay) (T2); and (4) TMR-2 supplemented with SCCF (T3). SCCF (5 ml/head, 2.0×10⁷ CFU/mL) was mixed with TMRs daily before feeding to dairy cows. The mean daily temperature-humidity index (THI) during this trial was 76.92 ± 0.51 on average and ranged from 73.04 to 81.19. For particle size distribution, TMR-2 had a lower >19 mm fraction and a higher 8-9 mm fraction than TMR-1 (p < 0.05). The type of TMR did not influence the DMI, body weight (BW), milk yield and composition, or blood metabolites. The milk yield and composition were not affected by the SCCF supplementation, but somatic cell counts were reduced by feeding SCCF (p < 0.05). Feeding SCCF significantly increased the DMI but did not affect the milk yield of dairy cows. The NEFA concentration was slightly decreased compared to that in the control and T2 groups without SCCF. Feeding a yeast culture of S. cerevisiae may improve the feed intake, milk quality and energy balance of dairy cows under heat stress.

• Wind and Structures
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• v.37 no.4
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• pp.255-274
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• 2023

The aim of this paper is to enhance the accuracy of predicting time-averaged external surface pressures on low-rise buildings by utilizing Computational Fluid Dynamics (CFD) simulations. To achieve this, benchmark studies of the Silsoe cube and the Texas Tech University (TTU) experimental building are employed for comparison with simulation results. The paper is structured into three main sections. In the initial part, an appropriate domain size is selected based on the precision of mean pressure coefficients on the windward face of the cube, utilizing Reynolds Averaged Navier-Stokes (RANS) turbulence models. Subsequently, recommendations regarding the optimal computational domain size for an isolated building are provided based on revised findings. Moving on to the second part, the Silsoe cube model is examined within a horizontally homogeneous computational domain using more accurate turbulence models, such as Large Eddy Simulation (LES) and hybrid RANS-LES models. For computational efficiency, transient simulation settings are employed, building upon previous studies by the authors at the Windstorm Impact, Science, and Engineering (WISE) Lab, Louisiana State University (LSU). An optimal meshing strategy is determined for LES based on a grid convergence study. Three hybrid RANS-LES cases are investigated to achieve desired enhancements in the distribution of mean pressure coefficients on the Silsoe cube. In the final part, a 1:10 scale model of the TTU building is studied, incorporating the insights gained from the second part. The generated flow characteristics, including vertical profiles of mean velocity, turbulence intensity, and velocity spectra (small and large eddies), exhibit good agreement with full-scale (TTU) measurements. The results indicate promising roof pressures achieved through the careful consideration of meshing strategy, time step, domain size, inflow turbulence, near-wall treatment, and turbulence models. Moreover, this paper demonstrates an improvement in mean roof pressures compared to other state-of-the-art studies, thus highlighting the significance of CFD simulations in building aerodynamics.