• 한국균학회소식:학술대회논문집
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• 한국균학회 2006년도 추계학술대회 및 정기총회
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• pp.10-12
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• 2006

• 한국식품영양학회지
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• 제22권4호
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• pp.492-496
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• 2009

The composition of the bacterial populations in Gorosoe(Acer mono Max.) sap was characterized during storage with different heat treatments($63^{\circ}C$ for 30 min and $73^{\circ}C$ for 15 sec). The saps were aseptically collected at 0, 15 and 30 days of storage and analyzed by dilution plating and 16S rDNA PCR-DGGE analysis. There were significant differences in the total number of colony forming units(CFUs) of bacteria between heated and nonheated saps. Bacteria of nonheated sap were present at a level of $3.4{\times}10^7CFU/m{\ell}^{-1}$, whereas living bacteria were not detected in the heated sap. According to the 16S rDNA sequence and DGGE analysis, Pseudomonas sp. was the most abundant bacterial strain in the samlpes, and the bacterial community structures become more simplified with time and were composed of the Chryseobacterium sp. with time. These results allowed us to characterize the dominant bacteria involved in Gorosoe sap and to better understand their dynamics throughout storage.

• The Plant Pathology Journal
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• 제18권5호
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• pp.231-236
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• 2002

Bemisia tabaci Genn. is an important pest worldwide because of its ability to cause damage by direct feeding and its role as a vector of some viruses including geminiviruses. The first report of Tobacco leaf curl virus (TLCV), a Geminiviruses, in Indonesia was in 1932 when the virus was found infecting tobacco plants in Central Java. The characteristic symptoms of TLCV included upward curling of the leaf edge, vein thickening, and sometimes the occurrence of enation on the underside of the leaves. Basic studies were carried out to elucidate the characteristics of TLCV transmission by its vector, B. tabaci. A single whitefly was able to transmit the virus and the efficiency of transmission was increased when the number of adult whiteflies was increased up to 20 per plant. Inoculation access period of 1 h could cause transmission up to 20% and the optimum inoculation access period was 12 h. Acquisition access period of 30 minutes resulted in 70% transmission while 1(10% transmission occurred with a 24-h acqui-sition access period. The virus was proven to be persistently but not transovarially transmitted. Discrete fragments of 1.6 kb were observed when polymerase chain reaction method was applied to detect the virus in viruliferous nymphs and individual adults of B. tabaci, while no bands were obtained from non-viruliferous nymphs and adults.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1644-1653
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• 2014

Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.

• 한국응용곤충학회지
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• 제56권1호
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• pp.41-49
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• 2017

2007년에 잣나무림에서 하늘소과, 바구미과, 나무좀아과에 속하는 천공성 딱정벌레 군집을 말레이즈 트랩을 활용하여 조사하였다. 하늘소류 15종 184 개체, 바구미류 17종 185개체, 6종 1,246개체의 총 1,615개체 천공성 딱정벌레가 채집되었고 우점종은 왕녹나무좀이었다. 개체수에 따라 순위를 매겼을 때, 잣나무림에서 천공성 해충과 나무좀류 군집은 나무좀아과의 한 종에 의해 우점되어 생물다양성이 낮은 것으로 나타났다. 잣나무림에서 간벌은 나무좀과 암브로시아좀류의 풍부도에 영향을 미쳐 특정 임분에서의 개체군들은 간벌 1년 후 밀도가 증가했으나 그 후 감소하였다.

• 한국응용곤충학회지
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• 제49권2호
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• pp.145-149
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• 2010

참나무시들음병 매개충인 광릉긴나무좀 (Platypus koryoensis (Murayama)) (Coleoptera: Platypodidae) 모니터링에 활용 가능한 트랩 종류, 색, 설치위치가 포획수에 미치는 영향이 조사되었다. 조사는 경기도 파주시 광탄면에서 수행되었으며 끈끈이 평판 트랩과 다중깔때기 트랩 두 종류 트랩이 이용되었다. 조사된 두 트랩의 종류는 광릉긴나무좀 포획수에 영향을 미치지 않았다. 끈끈이 평판 트랩의 경우 노란색, 검정색, 흰색, 투명색으로 시험한 결과 트랩색이 광릉긴나무좀 포획효율에 영향을 미치지 않았다. 끈끈이 평판 트랩과 다중깔때기 트랩 모두 수간내 경사 위쪽과 아래쪽에 설치된 경우, 산의 경사 위쪽에 설치한 트랩에 유의하게 많은 수의 광릉긴나무좀이 포획되었다. 이는 두 가지 트랩이 모두 광릉긴나무좀 모니터링 및 방제에 효율적으로 활용될 수 있음을 보여주는 것이다.

• International Journal of Internet, Broadcasting and Communication
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• 제15권2호
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• pp.133-143
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• 2023

In this study, we propose a big data set of strawberry pests collected directly for diagnosis model learning and an automatic pest diagnosis model architecture based on deep learning. First, a big data set related to strawberry pests, which did not exist anywhere before, was directly collected from the web. A total of more than 12,000 image data was directly collected and classified, and this data was used to train a deep learning model. Second, the deep-learning-based automatic pest diagnosis module is a module that classifies what kind of pest or disease corresponds to when a user inputs a desired picture. In particular, we propose a model architecture that can optimally classify pests based on a convolutional neural network among deep learning models. Through this, farmers can easily identify diseases and pests without professional knowledge, and can respond quickly accordingly.

• The Plant Pathology Journal
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• 제29권3호
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• pp.344-349
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• 2013

Intraspecific variation in morphometry of pine wood nematodes Bursaphelenchus xylophilus and B. mucronatus in relation to geographical locations in South Korea was investigated using morphometric characters (body length, a, b and c ratio, stylet length, and spicule length for a male nematode and V (%) value for a female nematode). B. xylophilus was isolated from Pinus thunbergii in Jinju (1998), Ulsan (2000), Yangsan (2000), Mokpo (2001) and Jeju (2004), and from P. densiflora in Gumi (2001). B. mucronatus was isolated from P. thunbergii in Jinju (1991) and from P. densiflora in Milyang (2001). The body length of male and female B. xylophilus had the highest coefficient of variability and showed significant differences among geographical locations. The V (%) value for female B. xylophilus showed the lowest coefficient of variability, changing little with geographical area and host plant. All morphometric characters in B. mucronatus except for stylet length and female body length showed no significant differences between locations or hosts, suggesting they may not be affected by geographical area or host plant.

• 한국산림과학회지
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• 제94권5호통권162호
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• pp.334-337
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• 2005

Pine wood nematode isolates from Haman, Gyoungsangnam-do were cryopreserved with three different cryoprotectants (glycerol, ethylene glycol and dimethylsulfoxide), Nematode Growth medium (NGM) and M 9 buffer solution, respectively, and then survival rate, reproduction ability, and pathogenicity of stored nematodes were compared. Survival rates of juvenile was excellent in 15% glycerol solution as $72{\pm}5.3%$. The survival ability of nematodes at juvenile stage was more efficient than that of nematodes at adult stage. Pre-incubation treatment at $4^{\circ}C$ before storing in liquid nitrogen didn't affect the survival ability of pine wood nematode. When the reproduction ability of nematodes was confirmed on Botrytis cinerea agar plates at $28^{\circ}C$ incubator, there was no difference between cryopreserved and non-cryopreserved. Pathogenic ability of cryopreserved nematodes was also evaluated and confirmed by artificially inoculating them on 2-year old red pine seedlings.

• The Plant Pathology Journal
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• 제32권4호
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• pp.340-346
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• 2016

To understand how Bursaphelenchus xylophilus kills pine trees, the differences between the effects of B. xylophilus and B. mucronatus on pine trees are usually compared. In this study, the migration and attacking ability of a non-pathogenic B. mucronatus in Pinus thunbergii were investigated. The distribution of B. mucronatus and the number of dead epithelial cells resulting from inoculation were compared with those of the pathogenic B. xylophilus. Although B. mucronatus is non-pathogenic in pines, its distribution pattern in P. thunbergii was the same as that of B. xylophilus. We therefore concluded that the non-pathogenicity of B. mucronatus could not be attributed to its migration ability. The sparse and sporadic attacking pattern of B. mucronatus was also the same as that of B. xylophilus. However, the number and area of the dead epithelial cells in pine cuttings inoculated with B. mucronatus were smaller than in those cuttings inoculated with B. xylophilus, meaning that the attacking ability of B. mucronatus is weaker than that of B. xylophilus. Therefore, we concluded that the weaker attacking ability of B. mucronatus might be the factor responsible for the non-pathogenicity.