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Analysis of the Bacterial Community during the Storage of Gorosoe(Acer mono Max.) Sap  

Oh, Jung-Hwan (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute)
Seo, Sang-Tae (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute)
Oh, Hye-Young (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute)
Hong, Jin-Sung (Institute of Nature Science, Seoul Women's University)
Kang, Ha-Young (Division of Forest Insect Pests & Diseases, Korea Forest Research Institute)
Publication Information
The Korean Journal of Food And Nutrition / v.22, no.4, 2009 , pp. 492-496 More about this Journal
Abstract
The composition of the bacterial populations in Gorosoe(Acer mono Max.) sap was characterized during storage with different heat treatments($63^{\circ}C$ for 30 min and $73^{\circ}C$ for 15 sec). The saps were aseptically collected at 0, 15 and 30 days of storage and analyzed by dilution plating and 16S rDNA PCR-DGGE analysis. There were significant differences in the total number of colony forming units(CFUs) of bacteria between heated and nonheated saps. Bacteria of nonheated sap were present at a level of $3.4{\times}10^7CFU/m{\ell}^{-1}$, whereas living bacteria were not detected in the heated sap. According to the 16S rDNA sequence and DGGE analysis, Pseudomonas sp. was the most abundant bacterial strain in the samlpes, and the bacterial community structures become more simplified with time and were composed of the Chryseobacterium sp. with time. These results allowed us to characterize the dominant bacteria involved in Gorosoe sap and to better understand their dynamics throughout storage.
Keywords
Gorosoe sap; dilution plating; 16S rDNA; DGGE;
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