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http://dx.doi.org/10.4014/jmb.1405.05017

Improvement of Wuyiencin Biosynthesis in Streptomyces wuyiensis CK-15 by Identification of a Key Regulator, WysR  

Liu, Yanyan (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences)
Ryu, Hojin (Microbial Crop Health Lab, Division of Agricultural Microbiology, National Academy of Agricultural Science, RDA)
Ge, Beibei (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences)
Pan, Guohui (State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences)
Sun, Lei (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences)
Park, Kyungseok (Microbial Crop Health Lab, Division of Agricultural Microbiology, National Academy of Agricultural Science, RDA)
Zhang, Kecheng (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.12, 2014 , pp. 1644-1653 More about this Journal
Abstract
Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.
Keywords
Wuyiencin; WysR; positive regulator; Streptomyces wuyiensis;
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