The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Transmission of an Indonesian Isolate of Tobacco leaf curl virus (Geminivirus) by Bemisia tabaci Genn. (Hemiptera: Aleyrodidae)

  • Noor, Aidawati (Department of Plant Pests and Diseases, Lambung Mangkurat University) ;
  • Sri, Hendrastuti Hidayat (Department of Plant Pests and Diseases, Lambung Mangkurat University) ;
  • Rusmilah, Suseno (Department of Plant Pests and Diseases, Lambung Mangkurat University) ;
  • Soemartono, Sosromarsono (Dept. of Plant Pests and Diseases, Bogor Agricultural University, Jalan Kamper Darmaga Compus)
  • Published : 2002.01.01
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Abstract

Bemisia tabaci Genn. is an important pest worldwide because of its ability to cause damage by direct feeding and its role as a vector of some viruses including geminiviruses. The first report of Tobacco leaf curl virus (TLCV), a Geminiviruses, in Indonesia was in 1932 when the virus was found infecting tobacco plants in Central Java. The characteristic symptoms of TLCV included upward curling of the leaf edge, vein thickening, and sometimes the occurrence of enation on the underside of the leaves. Basic studies were carried out to elucidate the characteristics of TLCV transmission by its vector, B. tabaci. A single whitefly was able to transmit the virus and the efficiency of transmission was increased when the number of adult whiteflies was increased up to 20 per plant. Inoculation access period of 1 h could cause transmission up to 20% and the optimum inoculation access period was 12 h. Acquisition access period of 30 minutes resulted in 70% transmission while 1(10% transmission occurred with a 24-h acqui-sition access period. The virus was proven to be persistently but not transovarially transmitted. Discrete fragments of 1.6 kb were observed when polymerase chain reaction method was applied to detect the virus in viruliferous nymphs and individual adults of B. tabaci, while no bands were obtained from non-viruliferous nymphs and adults.

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References

  1. Bendahmane, M., Schalk, H. J. and Gronenbom, B. 1995. Identification and characterization of wheat dwarf vims from Franceusing a rapid method for geminivirus DNA preparation. Phytopathology 85:1449-1455 https://doi.org/10.1094/Phyto-85-1449
  2. Bock, K. R. 1982. Geminivims diseases. Ptant Dis. 66:266-270 https://doi.org/10.1094/PD-66-266
  3. Bos, L. 1983. Introduction to Plant Virology, p. 159, LongmanPress, New York
  4. Brown, J. K. andNelson, R. 1988. Transmission, host range, and virus-vector relationships of Chinol del tomato virus, a white-fly-transmitted geminivims from Sinaloa, Mexico. Ptant Dis.72:866-869 https://doi.org/10.1094/PD-72-0866
  5. Butter, N. S. and Rataul, H. S. 1977. The virus-vector relationshipof the tomato leaf curl vims (TLCV) and its vector, Bemisiatabaci Gennadius (Homoptera; Aleyrodidae). Phytoparasitica 5:173-186 https://doi.org/10.1007/BF02980351
  6. Chiemsombat, P., K-ositratana, W., Attathom, S., Sutabutra, T. andSae-aung, N. 1990. DNA probe and nucleic acid hybridizationfor plant virus detection. Kasetsart J. (Nat. Sci. Suppl.) 24:12-16
  7. Cohen, S. and Nitzany, F. E. 1966. Transmission and host range ofthe tomato yellow leaf curl vims. Phytopathotogy 56:1127-1131
  8. Costa, A. S. 1969. WhiteIlies as virus vectors, pp. 95-119. In:Viruses, Vectors, and Vegetation, ed. Marasmorosch, K.,p659., John Wiley & Sons (Intel-science), New York
  9. Czosnek, H., Ber, R., Navol, N., Zamir, D., Antignus, Y. andCohen, S. 1988. Detection of tomato leaf curl'vims in lysatesof plants and insects by hybridization with a viral DNA probe.Plant Dis. 72:949-951 https://doi.org/10.1094/PD-72-0949
  10. Dellaporta, S. L., Wood, J. and Hicks, J. B. 1983. A plant DNAminipreparadon: Version II. PIantMoI. Biot. Rep. 1(4): 19-21
  11. Gilbertson, R. L., Hidayat, S. H. and Martinez, R. T. 1991. DiHer-entiation of bean-infecting gemmivimses by nucleic acidhybridization probes and aspects of bean golden mosaic inBrazil. PIant Dis. 75:336-342 https://doi.org/10.1094/PD-75-0336
  12. Goodwin, R. H., Xue, B. G., Kuske, C. R. and Sears, M. K. 1994. Amplification of plasmid DNA to detect plant pathogenic mycoplasmalike organisms. Ann. AppI. BioI. 132:27-36
  13. Greathead, A. H. 1986. Host plants, pp. 17-25. In: M. J. W. Cock(ed.), Bemisia tabaci A literature Survey on the Cotton White-fly with an annotated Bibliography. CAB International Insti-tute of Biological Control. Ascot, Berks, U.K
  14. Hidayat, S. H., Gilbertson, R. L., Hanson, S. R, Morales, F. J.,Ahlquist, R, Russel, D. R. and Maxwell, D. P. 1993. Completenucleodde sequences of the infectious cloned DNAs of beandwarf mosaic geminivirus. PhytopathoSogy 83:181-187 https://doi.org/10.1094/Phyto-83-181
  15. Idris, A. M. and Brown, J. K. 1998. Sinaloa tomato leaf curl gem-mivirus: Biological and molecular evidence for a new sub-group III virus. Phytopathology 88:648-657 https://doi.org/10.1094/PHYTO.1998.88.7.648
  16. Martin, J. H. 1987. An identification guide to common whiteflypest species of the wdfld (Homoptera: Aleyrodidae). Trop.PestManag. 33:298-322 https://doi.org/10.1080/09670878709371174
  17. Mehta, P. J., Wayman, J. A., Nakhla, M. K. and Maxwell, D. P.1994. Transmission of tomato yellow leaf curl gemimvims byBemisia tabaci (Homoptera: Aleyrodidae). J. Econ. Entomol.87:1291-1297 https://doi.org/10.1093/jee/87.5.1291
  18. Pacheco, I. T., Tiznodo, J. A. G., Brown, J. K., Flora, A. B. andBustamante, R. F. R. 1996. Detection and distribution of geminiviruses in Mexico and the Southem United State. Phytopa-thotogy 86:1186-1192 https://doi.org/10.1094/Phyto-86-1186
  19. Polston, J. E., Dodds, J. A. and Perring, T. M. 1989. Nucleic acidprobes for detection and strain discrimination of cucurbit gemhindi-uses. Phytopathology 79:1123-1127 https://doi.org/10.1094/Phyto-79-1123
  20. Rojas, M. R., Gilbertson, R. L., Russell, D. R. and Maxwell, D. P.1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Ptant Dis.77:340-347 https://doi.org/10.1094/PD-77-0340
  21. Roye, M. E., Mclaughlin, W. A., Nakhla, M. K. and Maxwell, D.P. 1997. Genetic diversity among geminiviruses associatedwith the weed species Sida spp., Macroptitium lathyroides, and Wissadula amplissima from Jamaica. PtantDis. 81:1251-1258
  22. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecularcloning. A laboratory manual. 2nd edition. Cold Spring Har-bour Laboratory Press, New York
  23. Srivastava, K. M., Singh, B. R, Dwadash Shreni, V. C. and Srivas-tava, B. N. 1977. Zinnia yellow net disease-transmission, hostrange, and agent-vector relationship. Ptant Dis. Reptr. 61:550-554
  24. Swenson, K. G. 1967. Plant vims transmission by insect, pp. 267-307. In: K. Maramorosch & H. Koprowski (eds.). Methods in Virology. Academic Press, New York
  25. Trisusilowati, E. B., Suseno, R., Sosromarsono, S., Barizi, Soe-dannadi and Nur, M. A. 1990. Transmission, serologicalaspects and morphology of the tobacco krupuk virus. Indon. J.Agric. 2:75-79
  26. Varma, P. M. 1963. Transmission of plant vimses by whiteflies.Nat. Inst. Sci. India Butt. 24:11-33
  27. Wyatt, S. D. and Brown, J. K. 1996. Detection of subgroup III geminivimses isolates in leaf extracts by degenerate primerand polymerase chain reaction. Phytopathotogy 86:1288-1293 https://doi.org/10.1094/Phyto-86-1288

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