Browse > Article
http://dx.doi.org/10.5423/PPJ.2002.18.5.231

Transmission of an Indonesian Isolate of Tobacco leaf curl virus (Geminivirus) by Bemisia tabaci Genn. (Hemiptera: Aleyrodidae)  

Noor, Aidawati (Department of Plant Pests and Diseases, Lambung Mangkurat University)
Sri, Hendrastuti Hidayat (Department of Plant Pests and Diseases, Lambung Mangkurat University)
Rusmilah, Suseno (Department of Plant Pests and Diseases, Lambung Mangkurat University)
Soemartono, Sosromarsono (Dept. of Plant Pests and Diseases, Bogor Agricultural University, Jalan Kamper Darmaga Compus)
Publication Information
The Plant Pathology Journal / v.18, no.5, 2002 , pp. 231-236 More about this Journal
Abstract
Bemisia tabaci Genn. is an important pest worldwide because of its ability to cause damage by direct feeding and its role as a vector of some viruses including geminiviruses. The first report of Tobacco leaf curl virus (TLCV), a Geminiviruses, in Indonesia was in 1932 when the virus was found infecting tobacco plants in Central Java. The characteristic symptoms of TLCV included upward curling of the leaf edge, vein thickening, and sometimes the occurrence of enation on the underside of the leaves. Basic studies were carried out to elucidate the characteristics of TLCV transmission by its vector, B. tabaci. A single whitefly was able to transmit the virus and the efficiency of transmission was increased when the number of adult whiteflies was increased up to 20 per plant. Inoculation access period of 1 h could cause transmission up to 20% and the optimum inoculation access period was 12 h. Acquisition access period of 30 minutes resulted in 70% transmission while 1(10% transmission occurred with a 24-h acqui-sition access period. The virus was proven to be persistently but not transovarially transmitted. Discrete fragments of 1.6 kb were observed when polymerase chain reaction method was applied to detect the virus in viruliferous nymphs and individual adults of B. tabaci, while no bands were obtained from non-viruliferous nymphs and adults.
Keywords
Bemisia tabaci; geminivirus; Tobacco leaf curl virus; transmission; vector;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bendahmane, M., Schalk, H. J. and Gronenbom, B. 1995. Identification and characterization of wheat dwarf vims from Franceusing a rapid method for geminivirus DNA preparation. Phytopathology 85:1449-1455   DOI
2 Czosnek, H., Ber, R., Navol, N., Zamir, D., Antignus, Y. andCohen, S. 1988. Detection of tomato leaf curl'vims in lysatesof plants and insects by hybridization with a viral DNA probe.Plant Dis. 72:949-951   DOI
3 Gilbertson, R. L., Hidayat, S. H. and Martinez, R. T. 1991. DiHer-entiation of bean-infecting gemmivimses by nucleic acidhybridization probes and aspects of bean golden mosaic inBrazil. PIant Dis. 75:336-342   DOI
4 Polston, J. E., Dodds, J. A. and Perring, T. M. 1989. Nucleic acidprobes for detection and strain discrimination of cucurbit gemhindi-uses. Phytopathology 79:1123-1127   DOI
5 Roye, M. E., Mclaughlin, W. A., Nakhla, M. K. and Maxwell, D.P. 1997. Genetic diversity among geminiviruses associatedwith the weed species Sida spp., Macroptitium lathyroides, and Wissadula amplissima from Jamaica. PtantDis. 81:1251-1258
6 Wyatt, S. D. and Brown, J. K. 1996. Detection of subgroup III geminivimses isolates in leaf extracts by degenerate primerand polymerase chain reaction. Phytopathotogy 86:1288-1293   DOI   ScienceOn
7 Greathead, A. H. 1986. Host plants, pp. 17-25. In: M. J. W. Cock(ed.), Bemisia tabaci A literature Survey on the Cotton White-fly with an annotated Bibliography. CAB International Insti-tute of Biological Control. Ascot, Berks, U.K
8 Idris, A. M. and Brown, J. K. 1998. Sinaloa tomato leaf curl gem-mivirus: Biological and molecular evidence for a new sub-group III virus. Phytopathology 88:648-657   DOI   ScienceOn
9 Swenson, K. G. 1967. Plant vims transmission by insect, pp. 267-307. In: K. Maramorosch & H. Koprowski (eds.). Methods in Virology. Academic Press, New York
10 Goodwin, R. H., Xue, B. G., Kuske, C. R. and Sears, M. K. 1994. Amplification of plasmid DNA to detect plant pathogenic mycoplasmalike organisms. Ann. AppI. BioI. 132:27-36
11 Hidayat, S. H., Gilbertson, R. L., Hanson, S. R, Morales, F. J.,Ahlquist, R, Russel, D. R. and Maxwell, D. P. 1993. Completenucleodde sequences of the infectious cloned DNAs of beandwarf mosaic geminivirus. PhytopathoSogy 83:181-187   DOI
12 Varma, P. M. 1963. Transmission of plant vimses by whiteflies.Nat. Inst. Sci. India Butt. 24:11-33
13 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecularcloning. A laboratory manual. 2nd edition. Cold Spring Har-bour Laboratory Press, New York
14 Bos, L. 1983. Introduction to Plant Virology, p. 159, LongmanPress, New York
15 Brown, J. K. andNelson, R. 1988. Transmission, host range, and virus-vector relationships of Chinol del tomato virus, a white-fly-transmitted geminivims from Sinaloa, Mexico. Ptant Dis.72:866-869   DOI
16 Cohen, S. and Nitzany, F. E. 1966. Transmission and host range ofthe tomato yellow leaf curl vims. Phytopathotogy 56:1127-1131
17 Martin, J. H. 1987. An identification guide to common whiteflypest species of the wdfld (Homoptera: Aleyrodidae). Trop.PestManag. 33:298-322   DOI   ScienceOn
18 Rojas, M. R., Gilbertson, R. L., Russell, D. R. and Maxwell, D. P.1993. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Ptant Dis.77:340-347   DOI   ScienceOn
19 Srivastava, K. M., Singh, B. R, Dwadash Shreni, V. C. and Srivas-tava, B. N. 1977. Zinnia yellow net disease-transmission, hostrange, and agent-vector relationship. Ptant Dis. Reptr. 61:550-554
20 Dellaporta, S. L., Wood, J. and Hicks, J. B. 1983. A plant DNAminipreparadon: Version II. PIantMoI. Biot. Rep. 1(4): 19-21
21 Costa, A. S. 1969. WhiteIlies as virus vectors, pp. 95-119. In:Viruses, Vectors, and Vegetation, ed. Marasmorosch, K.,p659., John Wiley & Sons (Intel-science), New York
22 Bock, K. R. 1982. Geminivims diseases. Ptant Dis. 66:266-270   DOI
23 Mehta, P. J., Wayman, J. A., Nakhla, M. K. and Maxwell, D. P.1994. Transmission of tomato yellow leaf curl gemimvims byBemisia tabaci (Homoptera: Aleyrodidae). J. Econ. Entomol.87:1291-1297   DOI
24 Pacheco, I. T., Tiznodo, J. A. G., Brown, J. K., Flora, A. B. andBustamante, R. F. R. 1996. Detection and distribution of geminiviruses in Mexico and the Southem United State. Phytopa-thotogy 86:1186-1192   DOI   ScienceOn
25 Butter, N. S. and Rataul, H. S. 1977. The virus-vector relationshipof the tomato leaf curl vims (TLCV) and its vector, Bemisiatabaci Gennadius (Homoptera; Aleyrodidae). Phytoparasitica 5:173-186   DOI
26 Chiemsombat, P., K-ositratana, W., Attathom, S., Sutabutra, T. andSae-aung, N. 1990. DNA probe and nucleic acid hybridizationfor plant virus detection. Kasetsart J. (Nat. Sci. Suppl.) 24:12-16
27 Trisusilowati, E. B., Suseno, R., Sosromarsono, S., Barizi, Soe-dannadi and Nur, M. A. 1990. Transmission, serologicalaspects and morphology of the tobacco krupuk virus. Indon. J.Agric. 2:75-79