• 한국임상수의학회지
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• 제35권2호
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• pp.39-45
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• 2018

We evaluated the effects of green tea extract (GTE) supplementation at different dilution steps on boar sperm freezing and in vitro fertilization. Sperm intracellular hydrogen peroxide ($H_2O_2$), motility, viability, acrosome integrity and morphology were determined. In addition, sperm IVF parameters (penetration and monospermy) and glutathione (GSH) levels of presumptive zygotes (PZs) were evaluated. Semen was diluted in lactose egg yolk (LEY) and cooled at $5^{\circ}C$ for 3 h (first dilution step) and then diluted in LEY with 9% glycerol and maintained at $5^{\circ}C$ for 30 min (second dilution step). Four experimental groups were compared: first and second dilution steps without GTE (control), first dilution step with GTE (Step 1), second dilution step with GTE (Step 2) and first and second dilution step with GTE (Step 1+2). The spermatozoa were frozen in nitrogen vapor. Higher sperm motility, viability and acrosome integrity after thawing were observed in Step 1, Step 2 and Step 1+2 groups compared with the control (P < 0.05). Lower $H_2O_2$ level was observed in Step 1+2 compared with control and Step 1 (P < 0.05). For IVF, matured oocytes were co-cultured with spermatozoa frozen according to the experimental groups. GSH levels of PZs were significantly higher in Step 2 and Step 1+2 than in control and Step 1 (P < 0.05) without a significant difference in IVF parameters. In conclusion, supplementation with GTE in both first and second dilution steps during the freezing process resulted in better boar sperm cryopreservation and might be beneficial for further embryo development.

• Preventive Nutrition and Food Science
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• 제13권4호
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• pp.334-339
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• 2008

This study was designed to determine the optimum conditions of heating temperature, heating time and dilution rates for producing heated garlic juice by using central composite design of response surface methodology. Garlic 3Department of Biosystems Engineering, Chungbuk National University, Cheongju 361-763, Korea was heated using a high temperature and pressure treatment apparatus. Total soluble solid contents ranged from 4.4 at $130^{\circ}C$ 3 hr and 6 fold dilution to 5.89 at $115^{\circ}C$ for 2 hr and 8 fold dilution. The highest total acidity was 0.55% at $120^{\circ}C$ for 3 hr and 2 fold dilution. The pH ranged from 4.01 at $130^{\circ}C$, 3 hr and 6 fold dilution to 5.85 at $120^{\circ}C$, 1 hr and 6 fold dilution. From the results of statistical analysis on the sensory evaluation the predicted optimum processing conditions for best color, taste, flavor and overall acceptance were $119.41^{\circ}C$, 3.11 hr, 5.85 fold dilution, $118.23^{\circ}C$, 3.57 hr, 3.15 fold dilution, $120.54^{\circ}C$, 3.47 hr, 5.01 fold dilution and $119.54^{\circ}C$, 3.18 hr, 5.66 fold dilution, respectively. The application of response surface methodology for preparing heated garlic juice processing showed a good correlation with high significance.

• 한국임상약학회지
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• 제7권2호
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• pp.86-90
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• 1997

This study was to find a more accurate method fur measuring small vancomycin dosages which are commonly used in neonates by comparing single and double dilution method. For single dilution method, 500 mg of vancomycin powder was accurately measured and reconstituted with 5 ml of distilled water to make a concentration of 100 mg/ml. Volumes of 0.05, 0.1, 0.15, 0.2, 0.3, 0.4, and 0.5 ml, which equal the target dosages of 5, 10, 15, 20, 30, 40, and 50 mg, were measured using syringes made by Shina and each sample was further diluted with 2 ml of $5\%$ dextrose. The solution of 100 mg/ml concentration was further diluted with $5\%$ dextrose to make a concentration of 20 mg/ml. Volumes of 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, and 2.5 ml, which correspond to 5, 10, 15, 20, 30, 40, and 50 mg, were sampled by the same Shina's syringe as in single dilution method and then each sample was further diluted to make a total volume of 10 ml. Each sample was analyzed by HPLC. The measured dosages of each sample in both single and double dilution methods were lower than the target dosages; however, e values in double dilution method were higher than those in single dilution method for seven target dosages. Percent target dosages in single dilution method were 65 to $90\%$, while in double dilution method 91 to $94\%$. Statistically significant difference between two groups was shown in 5, 10, 15, 20, and 40 mg dosages (p<0.05). In conclusion, when preparing small vancomycin dosages lower an 20 mg $(volume{\leq}0.2\;ml)$, using Shina's syringes, the double dilution method has a closer value to the target dosage than single dilution method.

• Tribology and Lubricants
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• 제26권4호
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• pp.240-245
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• 2010

This paper describes the influence on engine main bearing behavior of the oil film when the fuel is diluted on a diesel engine equipped with DPF system. Oil film pressure and the thickness is calculated in accordance to the fuel dilution. The calculation is based on the numerical analysis of the engine main bearing. As a result, the engine oil viscosity decreased as the fuel dilution increased. This led the increment of the maximum oil thickness pressure. Verification of the minimum oil film thickness settlement by the engine gas pressure and the fuel dilution was confirmed. Destruction possibility of the engine main bearing was foreseen when the engine speed was 2000 rpm with the fuel dilution 15% and the 5W40 engine oil.

• Asian-Australasian Journal of Animal Sciences
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• 제3권2호
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• pp.143-148
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• 1990

An experiment was conducted to compare the traditional weigh-suckle-weigh method and the $D_2O$ dilution technique to estimate milk consumption of suckling piglets. Milk consumption of 50 individual piglets was estimated on four consecutive days by the $D_2O$ dilution method and for approximately 8 hours on both the second and fourth day by the traditional WSW method. The average milk intake of piglets estimated by the $D_2O$ dilution method was 45.0 g/hr and there were no significant differences between the four measurement period. The traditional weigh-suckle-weigh method provided a significantly lower estimate of milk consumption (36.8 g/hr). However correction for weight losses associated with milk suckling and weighing would increase the weigh-suckle-weigh estimate to a level similar to that determined by the $D_2O$ dilution method.

• 한국입자에어로졸학회지
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• 제13권4호
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• pp.165-172
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• 2017

For precise particle measurements in combustion environments, various dilution sampling methods were compared. Dilution equipments using dilution tunnels and hot/cold dilution with porous tube dilutors were most frequently used so far. The combination of porous tube dilutor and ejector diluter has relatively small footprint, and it results in lower particle losses compared to other methods. To determine the portion of condensable particulate matter, proper temperature control and flow control is required.

• 한국해양환경ㆍ에너지학회지
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• 제3권1호
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• pp.35-44
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• 2000

1999년 2월에 관측한 수온 및 염분도 분포로부터 마산ㆍ창원 하수종말처리장의 해양 수중방류수에 대한 동계의 근역희석률을 산정하였다. 소조기의 약한 주변유속과 수온약층에 의해 하수플륨이 포착되어 희석률이 30～40정도로 저조했던 하계의 경우와는 달리 동계 소조기의 경우에는 주변해수의 수직적 등밀도혼합이 전 층에서 형성되어 하수플륨이 수표면까지 상승하며 희석률도 90～130정도로 훨씬 큰 것으로 나타났다. CORMIX2 모형의 모의결과에서도 소조기의 약한 유속(Ua=6.0cm/s)의 경우 동계 희석률이 하계 희석률보다 약 3배 정도 증가하였으며 대조기의 강한 유속(Ua=15.5cm/s)의 경우에는 두 계절의 희석률 차이가 30%정도로 미미하였다. 이는 수중방류 하수의 근역희석 및 혼합과정이 주변해수의 밀도성층 효과보다는 유동성분에 더 큰 영향을 받기 때문인 것으로 사료된다.

• Animal Bioscience
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• 제37권2호
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• pp.203-209
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• 2024

Objective: This study aimed to assess the impact of the dilution ratio of Tris diluent, storage at 0℃, and long-distance transportation on the spermatozoa of Simmental cattle. It also validated the feasibility of the regional distribution of fresh semen. Methods: In experiment 1, semen was diluted at four dilution ratios (1:6, 1:9, 1:12, and 1:15) to determine the optimal dilution ratio of Tris diluent. In experiment 2, we assessed sperm viability, progressive motility (objectively assessed by computer-assisted sperm analyzer), and acrosome intactness in Tris dilutions kept at constant 0℃ for 1, 3, 6, 9, and 12 days. We compared them to Tianshan livestock dilutions (Commercial diluent). In experiment 3, semen was diluted using Tris diluent, and sperm quality was measured before and after long-distance transport. Artificial insemination of 177 Simmental heifers compared to 156 using Tianshan Livestock dilution. Results: The outcomes demonstrated that 1:9 was the ideal Tris diluent dilution ratio. The sperm viability, Progressive Motility, and acrosome integrity of both Tris and Tianshan dilutions preserved at 0℃ gradually decreased over time. sperm viability was above 50% for both dilutions on d 9, with a flat rate of decline. The decrease in acrosome integrity rate was faster for Tianshan livestock dilutions than for Tris dilutions when stored at 0℃ for 1 to 6 days. There was no significant difference (p>0.05) in sperm viability between semen preserved in Tris diluent after long-distance transportation and semen preserved in resting condition. The conception rates for Tris dilution and Tianshan livestock dilution were 49.15% and 46.15% respectively, with no significant difference (p>0.05). Conclusion: This shows that Tris diluent is a good long-term protectant. It has been observed that fresh semen can be successfully preserved for long-distance transport when stored under 0℃ conditions. Additionally, it is feasible to distribute semen regionally.

• Water Engineering Research
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• 제3권2호
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• pp.93-111
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• 2002

In this paper, mixing characteristics and dilution of the merging buoyant discharges from array of multiple jets has been extensively studied in the hydraulic model experiments. New equations for dilution, which include the merging effects correctly, were derived. Experiments were constructed in a 20-m long, 4.9-m wide and 0.6-m deep flume, and the model diffuser was manufactured to indicate the typical characteristics of the existing ocean wastewater outfall in South Korea. Buoyant discharge from the diffuser was reproduced using heated water. Water temperature was measured using CC-Type thermocouple sensors, which were connected to a 40-channel data logger. Experimental results show that merging between ports in a particular riser is dependent upon the discharge densimetric Froude number, whereas merging between two ports which are facing each other at 90$\circ$ at the adjacent risers is dependent upon the discharge densimetric Froude number and distance from the port and port spacing. Centerline dilution increase with distance from the port outlet until two plumes has merged. However, after merging occurs, increase of the centerline dilution almost stops. Further distance from the position where merging occurs, centerline dilution increases again.

• 대한기계학회논문집B
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• 제28권1호
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• pp.1-8
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• 2004

An study has been performed with axisymmetric coflow diffusion flames to investigate the influence of air-side fuel side dilution and initial preheated temperature on the soot formation in methan/air flames. Soot quantities are determined by using PLII(Planar Laser Induced Incandescence), such a $C_2$H$_2$ major species(CH$_4$, $O_2$, $N_2$) and temperature are simulated by chemkin code. The numerical analysis was performed with transport properties and detailed reaction mechanisms m axisymmetric coflow diffusion flames. The study of how flame temperature and $N_2$ dilution of air and fuel side influence the soot concentrations is focused. Soot concentrations results on PLII show that preheated temperature contributes to an increase in the soot volume fraction, and soot formation Is more productive to air side dilution than to fuel side dilution. $C_2$H$_2$ concentrations have a similar tendency to soot concentrations.