• Journal of the Korean Society of Tobacco Science
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• v.11 no.1
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• pp.65-69
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• 1989

This study was conducted to obtain the breeding Information for varietal difference of leaf breakdown of flue-cured tobacco at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute In 1988. The experiment was designed in randomized block with 3 replications. And data were analyzed as spilt plot design composed with varieties for main and growth stages for sub-plots. Among the 8 varieties (NC 95, SL 72, Sleight G-28, TC 518, NC82, NC 2326, NC 567 and TC 499), first half derived from NC 95, and 4 latter varieties were used for check Plants and not derived from NC 95. Plant seedlings were transplanted in 15 April. The number of breakdown leaf were investigated twice at peak growth stage, 5 June and at early harvest stage, 30 June. NC 95 and varieties derived from NC 95 had showed significantly different on the number of breakdown leaf compared to the latter 4 ones after heavy rainfall, but there were not significantly different at the two growth stages. It was suggested that NC95 and varieties derived from NC 95 had substance to induce leaf breakdown by conditional genes after water absorption to plant tissue. Among the varieties derived from NC 95 cultivar SC 72, Speight G-28 and TC 518 had appeared significantly different on the number of breakdown leaf, respectively. These results could appreciated analogically that character of leaf breakdown were governed by heteromeric genes In NC 95.

• Korean journal of applied entomology
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• v.18 no.2 s.39
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• pp.73-76
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• 1979

Two different isolates of Xanthomonas oryzae, KB 7785 of pathotype I and JN 7721 of pathotype III, that had been the most virulent isolates in the previous inoculation test, were reisolated from cultivar 'Milyang 23' and serially transferred to 10 times. They were inoculated to the 3 cultivars; 'Milyang 23' in Kinmaze group, 'Yushin' in Kogyoku group and 'Tongil' in Rantai-emas group cultivars. It was observed that the virulence of the isolate JN 7721 was more attenuated by the serial transfer on the Wakimoto's agar than the isolate KB 7785. The attenuation of virulence of the isolate JN 7721 was more significant at the cultivar 'Milyang23' than at the other cultivars. This suggests that the host-pathogen interactions and differences of the pathogenicity-maintenance ability among the pathogenic strains may be involved.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.256-260
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• 2004

A major challenge facing those involved in the testing of new plant varieties for distinctness, uniformity and stability (DUS) is the need to compare new varieties against all those of common knowlege (reference varieties). One possible approach would be to group new (candidate) varieties and reference varieties using descriptions stored in databases prior to further of official test. testing. This study was carried out to manage a reference variety collection by databasing of hordein profiling. For this purpose, hordein subunits of the 48 National list barley (Hordeum vulgare L) cultivars were analysed. Total 22 of clear scorable hordein subunits were identified from D-subunit to B-subunit region and fifteen different hordein polypeptide patterns were obtained. Based on hordein subunit band pattern, UPGMA cluster analysis was conducted. Forty-eight cultivars were separated into three groups and genetic distance of cluster ranging from 0.55 to 1.00. Hordein subunits have a potential of selecting similar varieties compared with candidate varieties by controlling reference variety collection and playing an important complemental role in cultivar distinctness.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.224-230
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• 1996

Randomly amplified polymorphic DNA(RAPD) analysis was applied to detect the molecular polymorphisms in Gentiana scabra var. buergeri. A high level of molecular variability was found among wild plants and cultivars. In genetic analysis, eight of twenty primers were selected and 54 amplification products ranged 2. 2 to 0.2 kb were compared. Twenty - nine amplified products showed polymorphic, while five were monomorphic. Twenty of line specific bands were found. In genetic similarity and cluster analysis using PCR products, three wild plants collected from Naejangsan, Daedunsan and Keojedo and one cultivar Seochunjaerae were grouped into one cluster, while cultivar Jinbujaerae and Japanese line separated into another clusters, respectively. The identification of DNA polymorphisms by the RAPD technique will facilitate the selection of the lines from different origin.

• Journal of Mushroom
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• v.16 no.2
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• pp.79-85
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• 2018

This study was carried out to separately assess the production of post-culture, germinating, growing, and resting stages of Lentinula edodes grown in a smart cultivation facility based on ICT technology. The cultivation environment of the greenhouse facility was controlled to remain at different set points throughout a range of high ambient temperatures. Production of Sanjo-701-ho, which is a typical summer cultivar, and the new cultivar Sanjo-701-ho were tested in this ICT-based smart cultivation system. Sanjo-701-ho produced 353.7 g/bag of mushrooms from June to October, while Sanjo-701-ho produced 270.4 g/bag. These production amounts were stable over the high-temperature range of $20-35^{\circ}C$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.2
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• pp.157-166
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• 1997

This study was carried out with aim to find out a tall fescue cultivar which is palatable and resistant to hot climate, and a white clover cultivar which is less competitive with other grasses. Pastures with 3 treatments (TI: Fawn, tall fescue + Regal, white clover + orchardgrass + perennial ryegrass, T2: Roa, tall fescue + Regal, white clover + orchardgrass + perennial ryegrass, T3 : Roa, tall fescue + Tahora, white clover + orchardgrass + perennial ryegrass) were established by oversowing. Under continuous grazing, dry matter yield, soil characteristics, botanical composition and nutrient contents of forages were investigated during the grazing seasons 60m 1994 to 1996. No significant difference were found in establishment percents, physical and chemical characteristics of soil, and nutrient contents of forages between 3 treatments(P>0.05). Dry matter yield of TI, T2 and T3 were 24,188, 23,827 and 23,578kg/ha, respectively. Fawn and Roa, tall fescue cultivars occupied 6.1 and 4.8% of the total plants in 1994, 14.4 and 11.5 % in 1995, and 28.4% and 17.7% in 1996, respectively. These results indicate that Fawn, tall fescue dominantly occupied with time more than Roa, tall fescue. There were no differences in percentage of Regal and Tahora, white clover cultivars(P > 0.05), showing 4.2 and 2.4% in 1994, 16.1 and 17.3% in 1995, and 2.4 and 1.0% in 1996, respectively. The percentage of orchardgrass on mixed pasture decreased after summer season of high temperature, but percentage of Roa, tall fescue slowly increased with time firom 1994 to 1996.

• ALGAE
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• v.33 no.1
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• pp.101-108
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• 2018

Saccharina japonica was introduced to both Korea and China from Hokkaido, Japan, and it has become an economically important species in both nations. After a long period of cultivation, several varieties of S. japonica have been developed in Korea and China. In this study, we conducted aquacultural research on the persistence of thalli from two kelp cultivars, one from China (Huangguan No. 1) and one from Korea (Sugwawon No. 301), between December 2015 and November 2016 in Haenam, Korea. The maximum length was $247.8{\pm}13.0$ and $227.5{\pm}42.0cm$, respectively, which were significantly longer in Sugwawon No. 301 than in Huangguan No. 1. The maximum width was $29.9{\pm}5.4$ and $23.2{\pm}1.9cm$, respectively, which were significantly wider in Huangguan No. 1 than in Sugwawon No. 301. The mean biomass obtained from the culture ropes was for Sugwawon No. 301 was $3.5{\pm}0.3kg\;wet\;wt\;m^{-1}$ and for while Huangguan No. 1 was $3.1{\pm}1.0kg\;wet\;wt\;m^{-1}$ of culture rope. After August, the persistence of the thalli of Sugwawon No. 301 was two months longer than that of Huangguan No. 1. We found that the Sugwawon No. 301 performed as well as the Huangguan No. 1 in Korean waters possibly due to increased flexibility as a result of the different cell arrangements of the two cultivars. Overall, the use of the Sugwawon No. 301 cultivar rather than the Huangguan No. 1 cultivar of S. japonica appears the best alternative to help to ensure a stable year round algal feed supply for the Korean abalone industry.

• Research in Plant Disease
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• v.13 no.3
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• pp.137-144
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• 2007

Fifty nine strains of Agrobacterium vitis, the causal agent of crown-gall disease on grapevine, originating from different geographical regions and 16 grapevine cultivars including 35 Kyoho cultivar of Korea, were characterized by PCR polymorphic analysis using Universal Rice Primer(URP). Of 12 URP primers, primers URP1F, URP2R, URP2F, and URP4R, URP17R were available for detecting PCR polymorphic bands among the A. vitis strains. PCR polymorphic bands produced by primers URP2F and URP17R were profiled to 12 strain types. A. vitis strains originated from Kyoho cultivar of grapevine showed relatively simple genetic diversify of the four PCR types, while the A. vitis strains originated from other grapevine cultivars and type culture strains showed various genetic diversity with 8 types. Unweighted Pair-Group Method with Arithmetic mean(UPGMA) cluster analysis using the URP-PCR polymorphic bands showed 59.4. vitis strains are genetically clustered into large seven groups.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.119-119
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• 2017

Growth related to morphological and proteome response under waterlogging stress in sorghum has not yet been elucidated. Understanding how plants respond to waterlogging, the present study was conducted in seedlings leaf of the Nam-pung chal cultivar. Regarding 3-leaf stage of sorghum, stem length and plant height were slightly decreased in the treatments during ten days of waterlogging, and chlorophyll contents were also significantly different from 7 days of waterlogging treatment. The results observed from the present study were considered to be influenced by the waterlogging stress more in the $5^{th}$ leaf stage of the growth period of the sorghum, and as the waterlogging treatment progressed, the waterlogging stress gradually influenced the growth difference between the control and the treatment respectively. Using 2-DE method, a total of 74 differentially expressed protein spots were analyzed using LTQ-FT-ICR MS. Of these proteins, 45 proteins were up-regulated in the treatment group, and 32 proteins were down-regulated. Analysis of LTQ-FI-ICR MS showed that about 50% of the proteins involved in carbohydrate metabolic process, metabolic process, and cellular metabolic compound salvage were affected by stress. Malate dehydrogenase protein and Glyceraldehyde-3-phosphate dehydrogenase protein related to carbohydrate metabolic process increased the level of protein expression in both 3 and 5-leaf stage under waterlogging stress. The increased abundance of these proteins may play an active role in response to waterlogging stress. These results provide new insights into the morphological alteration and modulation of differentially expressed proteins in sorghum cultivar.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.120-120
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• 2017

Since the composition of proteins from the Korean cultivars of Proso millet is unknown; thereby, the present study was conducted to obtain a reference map of millet seed proteins and identify the functional characteristics of the identified proteins. Proteins extracted from the millet seeds of various cultivars, were investigated using proteomic techniques as 2D electrophoresis coupled with mass fingerprinting. The 1152 (differentially expressed) proteins were detected on 2-D gel. Among them, 26 reproducible protein spots were analyzed by MALDI-TOF-TOF mass spectrometry. Out of 26 proteins, 2 proteins were up-regulated towards all cultivars of millet, while 7 proteins were up-regulated and 13 proteins were down-regulated against only one cultivar. However, abundance in most identified protein species, associated with metabolism, transcription and transcription was significantly enhanced, while that of another protein species involved in polysaccharide metabolism, stress response and pathogenesis were severely reduced. Taken together, the results observed from the study suggest that the differential expression of proteins from the four cultivars of millet may be cultivar-specific. Taken together, a proteomic investigation of millet seeds from different cultivars, we sought to better understand the genetic variation of millet cultivars representing the future millet research, and the functional categorization of individual proteins on the basis of their molecular function.