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Hordein Fingerprinting for Cultivar Discrimination in National List of Barley  

소은희 (국립종자관리소 재배시험과)
고은별 (국립종자관리소 재배시험)
최수정 (국립종자관리소 재배시험과 밀양지)
이종호 (국립종자관리소 재배시험과 밀양지)
송인호 (국립종자관리소 재배시험과)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.49, no.3, 2004 , pp. 256-260 More about this Journal
Abstract
A major challenge facing those involved in the testing of new plant varieties for distinctness, uniformity and stability (DUS) is the need to compare new varieties against all those of common knowlege (reference varieties). One possible approach would be to group new (candidate) varieties and reference varieties using descriptions stored in databases prior to further of official test. testing. This study was carried out to manage a reference variety collection by databasing of hordein profiling. For this purpose, hordein subunits of the 48 National list barley (Hordeum vulgare L) cultivars were analysed. Total 22 of clear scorable hordein subunits were identified from D-subunit to B-subunit region and fifteen different hordein polypeptide patterns were obtained. Based on hordein subunit band pattern, UPGMA cluster analysis was conducted. Forty-eight cultivars were separated into three groups and genetic distance of cluster ranging from 0.55 to 1.00. Hordein subunits have a potential of selecting similar varieties compared with candidate varieties by controlling reference variety collection and playing an important complemental role in cultivar distinctness.
Keywords
Hordeum vulgare L; DUS test; Hordein subunit; SDS-PAGE;
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