• 농업과학연구
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• 제46권3호
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• pp.549-557
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• 2019

Staphylococcal enterotoxin is a very common cause of food poisoning. Conventional detection methods for the toxin including enzyme-linked immunosorbent assays (ELISAs), chemiluminescence (ECL), and polymerase chain reaction (PCR) assays require a lot of time, efforts, and expert technicians. Lateral flow strip kits have shown great potential for the rapid detection of foodborne pathogens. The lateral flow strip kit is widely used in clinical settings because it is easy to use, fast, and cost effective. A typical lateral flow strip kit uses colloidal gold to generate a visual signal. However, the lateral flow strip kit based on colloidal gold has limited sensitivity to fulfill food safety regulation requirements. This study was performed to develop a rapid test kit for pathogenic staphylococcal enterotoxin B (SEB) in food samples. The rapid detection kit was fabricated based on a nitrocellulose lateral-flow strip. Cellulose nano beads and SEB antibodies were used as the tag and receptor, respectively, to improve the detection performance. Manually spotted SEB antibody and anti-rabbit antibody on the surface of the nitrocellulose membrane were used as test and control spots, respectively. The feasibility of the rapid test kit to detect SEB in samples was evaluated. The sensitivity of the kit was 10 ng/mL SEB spiked in PBS. Additionally, the rapid test kit could detect 1 ng/mL of SEB in chicken meat extract.

• 한국수정란이식학회지
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• 제32권3호
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• pp.153-157
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• 2017

This study was conducted to investigate the optimal artificial insemination (AI) time with diagnostic kit at ovulation time. We already applied the patent about the protein in the cow heat mucose in external reproductive tract. And we would examine the accuracy for detection of cow heat by the kit produced with the protein. Evaluation of optimal heat detection was tried two time at 12 hrs and 24 hrs after the heat. And then, AI service also performed two times with no relation to the results of heat diagnosis by heat detection kit and pregnancy rates were checked with rectal palpation on $60^{th}$ day after AI. Heat diagnostic results by kit in natural heat after 12 hrs in Hanwoo cows were showed 31.3~75.0% on positive in first heat detection and 33.3~100.0% on positve in second heat detection. In the $1^{st}$ positive results were significant different (p<0.05), but $2^{nd}$ positive were not. The results of heat detection showed different result on regional influence and individual cow effects. The pregnancy rates of first trial of heat detection were showed 34.4~78.7% on positive and 21.3~68.8% on negative after the diagnosis by heat detection kit. And the pregnancy rates of next trial of heat detection were showed 33.3~85.7% on positive and 14.3~66.6% on negative after the heat diagnosis. Both positive results of first trial and next trial also were showed significant different (p<0.05), but negative results were not. In positive result, first trial of total pregnancy rates was higher than the next trial of pregnancy, but there showed opposite results on negative results. In conclusion, the optimal heat detection kit is suitable to ordinary Hanwoo cows and it suggested that we have to improve the kit's accuracy by detecting the materials like proteins related optimal AI time.

• 한국군사과학기술학회지
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• 제22권4호
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• pp.575-580
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• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• 한국환경과학회지
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• 제17권12호
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• pp.1325-1330
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• 2008

This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was $162{\mu}$mol/min/g protein. $I_{50}$ for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at $4^{\circ}C\;and\;25^{\circ}C$ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.

• Toxicological Research
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• 제20권2호
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• pp.101-108
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• 2004

유전자변형작물의 이점과 잠재적 위해에 관한 다른 사회적 인식은 각국에서 다른 반응을 유발시켜왔다 한국을 포함한 많은 국가는 새로운 규제를 제정하기 위해 부심하고 있다 한국은 최근에 3% 이상의 유전자변형작물 혼입을 포함하는 모든 식품에 표시제를 실시하였다. 유전자변형작물 혼입을 신속하고 간편하게 검출하는 방법의 하나는 PCR에 의한 도입 DNA의 증폭이다 이 목적을 위한 많은 PCR kit가 시판되고 있어, 본 연구는 이들 상업화된 유전자변형작물 검출 kit의 성능을 시험하였다. 그 결과 이들 15개 kit 중 6개는 안정성, 재현성 및 검출 한계의 측면에서 제작사 스스로 제시한 요구조건도 충족하지 못하여 이들 kit의 개발 및 생산 단계에서 품질관리에 문제점이 있음을 시사하였다 본 시험은 또한 duplex와 triplex검출 kit가 단일 PCR 반응에서 명백한 검출을 보장할지라도, monoplex 검출 kit의 검출 능력이 가장 높다는 것을 시사하였다. 또한, kit들은 콩과 옥수수 사이에서 다른 검출 한계를 보였다. 본 연구의 결과는 GM 작물의 재배, 국가간 이동, GM 작물을 사용한 식품 생산 과정의 모니터링 뿐만 아니라 GM작물과 관련한 정부의 법규를 준수하기 위한 GM작물의 혼입의 건전한 과학적 추적체계의 개발에 유용할 것이라 사료된다.

• 대한바이러스학회지
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• 제27권2호
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• pp.137-141
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• 1997

We evaluated Immunochromatographic assay kit to screen HBsAg in human serum. When the reference HBsAg was applyed to ICA, HA and EIA kits, the limit of detection for HBsAg were found out to be 4, 2 and 0.25 ng/ml respectively. But ICA kit required 5 minutes to read the result whereas HA and EIA kit more than one hour. The sensitivity was 97% (29 of 30 samples) and the specificity 100% (45 samples) compared with conventional EIA. The ICA kit needs no instrument or machine to perform the test contrary to the conventional methods. Therefore, this rapid and sensitive ICA kit can be used for HBsAg-screening, especially in the emergency room and in the scene of the accident.

• 한국식품위생안전성학회지
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• 제18권3호
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• pp.118-124
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• 2003

식품으로부터 다양한 병원 미생물을 신속 검출하기 위하여 다양한 검출 원리를 이요한 키트들이 개발 시판되고 있다. 검사키트는 신속, 정확하고 간단하게 사용할 수 있으므로 검사기관이나 실험실 뿐 아니라 식품회사에서 QC 또는 QA를 수행하기 위하여 사용이 증가되고 있는 추세이다. 이에 본 연구에서는 E. coli 0157:H7의 단클론항체를 이용하여 면역크로마토글래피법에 의해 개발된 E. coli 0157:H7 검출 키트(Donga Co, Korea, D-kit)에 대한 검출감도 및 특이성을 확인하고 식품 시료에 적용 가능성을 평가하였다. 면역크로마토그래피법에 의하여 개발 시판되고 있는 Reveal E. coli 0157:H7 kit (Neogen Co., USA. R-kit)와 VIP EHEC kit(Biocontro Inc., USA. V-kit)를 비교 키트로 사용하였다. E. coli 0157:H7 표준균주를 사용하여 실시한 검출감도 확인시험 결과 R-kit 및 D-kit는 104/m/의 농도에서 양성으로 확인되었고 $10^3$/ml에서도 약한 양성 반응을 보였으나, V-lit는 $10^5$/ml농도로 검출감도가 낮았다. 또한, 배양액을 가열하여 kit에 적용하는 것이 가열하지 않은 경우보다 검출감도를 높일 수 있었다. E. coli 0157:H7 분리 22주, verotoxin 생성 E. coli 7주 E. coli 분리주 40주 중 3주를 제외한 모든 균에서 음성의 결과를 보여 특이성을 확인하였다. 세 키트에 위양성 반응을 보인 것은 E. coli 0157:H19, E. coli 0148:H18 및 Salmonella gallinarium으로 이들 혈청형과 0157:H7 사이에는 유사한 혈청학적 특성이 존재하는 것으로 추정되었다. 이상의 실험결과로 D-kit는 E. coli 0157:H7을 검출하는데 감도 및 특이성 면에서 기존 키트인 R-kit 및 V-kit와 같이 이용한 것으로 확인되었다.

• 분석과학
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• 제29권1호
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• pp.43-48
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• 2016

법과학 분야에서 다양한 Y-STR 분석 키트가 개발되어 사용되고 있고, 새로운 키트의 법과학적 적용에 앞서 DNA 감정에 적절한 분석 키트들의 선정과 표준작업절차서의 작성을 위해 실험실 내의 내부적 유효성 검증 및 민감도 시험은 필수적인 과정이다. 본 논문에서는 새로운 상업용 키트인 Yfiler^® PLUS PCR Amplification Kit (Yfiler plus 키트, 2014년 출시)를 AmpF/STR^® Yfiler^TM PCR Amplification Kit (Yfiler 키트, 2004년 출시)와 비교함으로써 민감도에 대한 연구를 수행하였다. Yfiler plus 키트는 Yfiler 키트의 17 개 Y-STR 좌위를 포함하면서 새로운 10 개의 Y-STR 좌위가 추가되었다. 먼저, 표준 DNA 시료인 2800M, 007을 이용하여 두 키트 간의 민감도 차이를 분석하였고, 선별된 0.5 ng 미만의 법과학 현장시료 16 개로부터 검출률을 비교하였다. 그 결과, Yfiler 키트보다 Yfiler plus 키트가 높은 민감도와 검출률을 보였고, 더 많은 좌위에서 Y-STR 프로필을 얻을 수 있었다. 이러한 결과들로부터 낮은 농도의 법과학 현장시료에서 Yfiler plus 키트가 Y-STR 프로필을 검출하는데 더욱 효과적인 분석키트임이 확인되었다.

• 대한임상검사과학회지
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• 제42권2호
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• pp.86-91
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• 2010

We evaluated the performance of a novel screening test, PBP2a MRSA rapid kit (Dinona Inc., Iksan, Korea), for methicillin-resistant Staphylococcus aureus (MRSA) based on a immunochromatographic assay. The test is able to detect penicillin-binding protein 2a (PBP2a) using the nasal specimens from health care workers. The nasal specimens were obtained from 69 healthcare workers and were incubated in enrichment broth followed eight hours incubatin in BHI with cefoxitin $4{\mu}g/mL$. These broth were tested by PBP2a Rapid Kit. The enrichment broths were also directly tested for tube coagulase using the conventional identification method. 19 of 22 MRSA showed positive results by PBP2a rapid test and direct coagulase test (the sensitivity for detection of MRSA, 86.36%). While, 8 of 47 non-MRSA showed false positive results for the two tests. All of the 8 non-MRSA which showed false positive were co-colonizing isolates with MRCNS and MSSA. In addition, 46 of 49 methicillin-resistant staphylococci (MRS) showed positive results for PBP2a MRSA rapid kit (the sensitivity for detection of MRS, 93.8%), and all of 20 non-MRS showed negative results (specificity, 100%). The combination of PBP2a MRSA rapid kit and direct coagulase test showed the good sensitivity for detection of MRSA from anterior nares but frequently showed false positive results from the co-colonizing carrier with MRCNS and MSSA.

• 대한수의학회지
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• 제58권1호
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• pp.27-31
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• 2018

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between $1.97{\times}10^4/mL$ and $9.85{\times}10^3/mL$ for samples with a 2-fold serial dilution from $1.25{\times}10^6\;TCID_{50}$ ($TCID_{50}$, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.