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Performance Evaluation of PCR Kits for Detecting Genetically Modified Crop Ingredients  

윤시온 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
정순천 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
윤원기 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
박상규 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
문제선 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
이정현 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
김환묵 (한국생명공학연구원 바이오평가센터 LMO 평가연구실)
Publication Information
Toxicological Research / v.20, no.2, 2004 , pp. 101-108 More about this Journal
Abstract
The different social reflections about the benefits and the potential risks of genetically modified (GM) crops have evolved with .different reactions in different countries. Many countries including Korea are working toward setting down new guidelines. Korea requires companies to label all food that contains more than 3% GM ingredients. One of the rapid and convenient detection methods of GM ingredients is amplification of the introduced DNAs by polymerase chain reaction (PCR). Many PCR kits for this purpose are commercially available. The objective of this study was to evaluate performance of commercialized GM crop detection kits. The results showed that 6 out of 15 kits tested did not meet the requirements even purposed by the manufacturers themselves in terms of stability, reproducibility, and detection limits, suggesting a potential quality control problem in their design stage or production line. The evaluation also suggests that, although the duplex and triplex detection kits allowed unambiguous detection in a single PCR reaction, the monoplex detection kits were the most sensitive to the detection of GM ingredients. The detection limits also differ between soybean and corn. Results from this study will be useful in the development of sound qualitative tracking systems of GM ingredients for monitoring throughout the cultivation of GM crops, their trans-boundary movement, and food production using GM grains as well as for complying with government guidelines associated with GM crops.
Keywords
Corn; Genetically modified crop; PCR detection kit; Soybean.;
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