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Effects of Fasting and High-fat Diet Feeding on Uncoupling Protein 3 mRNA Levels of Skeletal Muscle in Rats (절식과 고지방식 섭취가 골격근 UCP3 mRNA 발현에 미치는 영향)

  • Lim, Kiwon;Hwang, Hye-Jung;Suh, Heajung;Tamura, Tomohiro
    • Korean Journal of Exercise Nutrition
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    • v.13 no.2
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    • pp.155-160
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    • 2009
  • PURPOSE. The purpose of this study was to investigate the effects of fasting and high-fat diet feeding on uncoupling protein 3 (UCP3) mRNA levels, uncoupling the respiratory chain and producing heat, of skeletal muscle in rat. METHODS. Fasting experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into non-fasting groups (CON) and fasting groups (FG) for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of CON were sacrificed at 0 and 3 day. High-fat diet experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into low-fat diet groups (LF) and high-fat diet group feeding for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of LF were sacrificed at 0 and 3 day. Analysis: Analysis of UCP3 mRNA expression was used by Real-time PCR. RESULTS. UCP3 mRNA levels of FG group were increased according to time course for 2 days- fasting but decreased at 3 day-fasting. UCP3 mRNA of HF were increased during HF diet feeding for 2 day, and peaked at 1 day-HF feeding, but decreased 2 day and 3 day-HF feeding CONCLUSION. Therefore, it may be rational that UCP3 is up-regulation when a large amount of fatty acids influx occurs in skeletal muscles as well as might have a role for fine adjustments of energy expenditure.

Prediction of Parturition Day by Determination of Plasma Progesterone Concentrations in Companion Bitches 1. To Estimate of Prediction of Parturition Day (반려견에서 혈중 Progesterone 농도 측정에 의한 분만일 예측 1. 분만예정일의 산정)

  • Lee, Ju Hwan;Son, Chang Ho
    • Journal of Veterinary Clinics
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    • v.37 no.6
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    • pp.301-304
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    • 2020
  • The gestation period and parturition days were accurately predicted by measuring progesterone concentration in the plasma from 40 pregnant companion bitches. The mean length of the estrous cycle based on plasma progesterone concentrations were 8.14 ± 1.39 (Mean ± SD) days for proestrus, 9.19 ± 2.01 days for estrus, and 55.38 ± 1.96 days for diestrus phase, respectively. The gestation length from each based on the days was 65.61 ± 2.47 days from the first day of estrus after the first vaginal discharge, 63.21 ± 0.99 days from the day when plasma progesterone concentrations increase above 4.0 ng/ml, and 54.51 ± 3.51 days from the first day of diestrus, respectively. Therefore, the parturition day was estimated 65 days from the first day of estrus after the first vaginal discharge, 63 days from the day when plasma progesterone concentrations increase above 4.0 ng/ml, and 54 days from the first day of diestrus, respectively.

The Application of Customized Evidence-based Counseling for Abused Preschool Children in Day Care Centers: Focusing on the Multidisciplinary Cooperative Linking System (보육현장 아동학대에 대한 근거기반 맞춤형 개입 모델 개발 및 적용: 다학제간 협력적 연계 시스템을 중심으로)

  • Lee, Kyung-Sook;Park, Jinah
    • Korean Journal of Childcare and Education
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    • v.16 no.1
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    • pp.1-20
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    • 2020
  • Objective: The purpose of this study was to explore the application of the multidisciplinary model, which is customized evidence-based counseling for abused preschool children done by a day care center teacher. Methods: The participants were 16 preschool children which were abused by a day care center teacher and their parents. The data were analyzed by using the Wilcoxon Signed-rank test. Results: The multidisciplinary cooperative service model based on customized evidence-based counseling for abused preschool children, their parents, and day care center teachers was developed. Based on this model, customized evidence-based Post-Traumatic Stress Disorder (PTSD) emergency intervention was provided to preschool children abused by a day care teacher in Incheon. The multidisciplinary cooperative emergency intervention service proved to be effective in reducing emotional and behavioral problems of abused preschool children and enhancing the mental health of parents. Conclusion/Implications: The results of this study indicated that the customized evidence-based counseling for abused preschool children done by a day care center teacher using the multidisciplinary cooperative linkage system would be effective, and that child abuse prevention by day care center teachers and intervention services should be provided systematically at the national level.

A Study on the Stage of Embryos Non-Surgically Recovered from Heifers and Cows in Natural Heat (자연배란된 처녀우와 경산으로부터 비외과적으로 회수한 수정란의 발육단계에 관한 연구)

  • 정구민;김종국;임경순
    • Journal of Embryo Transfer
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    • v.4 no.1
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    • pp.41-45
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    • 1989
  • Total thirty of flushing were attempted on day 4 to 15 of estrus cycle with S heifers and 9 cows by nonsurgical method. The flushed or recovered rate among flushings was 86.7% (26/30) or 88.5% (23/26), respectively. There was no difference in the recovered rate between heifers (85.7%,6/7) and cows (89.5%, 17119). The embryo was recovered on day 4 to 15 of estrus cycle from the donors in natural heat without any technical difficulties.The I2FG Foley catheter used for pubertal heifers had sometimes plug in it with uterine mucus during flushing of uterine horn. But the problem could be overcomed by pumping the catherter with fluthing solution or by changing the catheter. Three normal embryos were recovered from 3 pubertal (10-11 month old) heifers. The rate of normal and abnormal eggs was 60.9% (14123) and 39.1% (9/23), respectively. The abnormal eggs were on degenerating except one unfertilized egg and were mostly recovered from heifers or cows flushed consecutively during the estrus cycle. The developmental states of normal embryos were l6-cells on day 5, 32-cells on day 6, compacted-morula on day 7, early-to expanded-blastocyst on day 8-to 9, and hatching-to hatched-blastocyst on day 10 to 11 of estrus cycle. The stage of embryos on day 8 to 10 showed varities among donors. On day 8 to 9 of estrus cycle hatching-blas tocyst was recovered from some donors.

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Clinical Effects of Indirect Moxibustion Treatment with General Acupuncture on HIVD Patients in Comparison with General Acupuncture Only (요추추간판탈출증에 대한 일반침 치료와 간접구 병행치료의 임상적 효과비교)

  • Kim, Min-Seok;Park, Jae-Yeon;Choi, You-Jin;Yoon, Kyoung-Jin;Jeon, Jae-Cheon;Lee, Tae-Ho;Lee, Eun-Yong;Roh, Jeong-Du
    • Journal of Acupuncture Research
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    • v.28 no.1
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    • pp.65-75
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    • 2011
  • Objectives : The purpose of this study was to evaluate the clinical effect of indirect moxibustion treatment for HIVD patients. Methods : From March 2010 to December 2010, 32 HIVD patients who admitted to Semyung oriental medical hospital were divided into two groups. Group I was treated by indirect moxibustion and general acupuncture, group II by general acupuncture. We evaluated the treatment effect of each group with the visual analog scale(VAS) and Oswestry disability index(ODI) by dividing three period(from admission day to third day after admission, from third day to sixth day after admission and from sixth day to ninth day after admission). Results : 1. Group I was more effective than group II in VAS score reducing rate at sixth day after admission and ninth day after admission but there was no statistical significance between two groups at third day after admission 2. Group I was more effective than group II in VAS improvement rate from third day to sixth day after admission but there was no statistical significance between two groups from admission day to third day after admission and sixth day to ninth day after admission. 3. Group I was more effective than group II in ODI score reducing rate at sixth day after admission and ninth day after admission but there was no statistical significance between two groups at third day after admission. 4. In ODI improvement rate group I was higher than group II, but there was no statistical significance. Conclusions : Through this research, Indirect moxibustion treatment is considered to be effective reducing pain for HIVD(Herniated intervertebral disc) patients.

Needs of Day-care Staff for a Center-based Child Care Health Program II (보육시설 영유아의 건강관리를 위한 특수보육 요구조사 II - 보육교사의 요구를 중심으로 -)

  • Han, Kyung-Ja;Choi, Mi-Young;Kim, Ji-Soo
    • Child Health Nursing Research
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    • v.13 no.2
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    • pp.128-135
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    • 2007
  • Purpose: To investigate health problems in child day care centers, needs for Child Care Health Programs, and management of sick children by day-care staff. Methods: A cross-sectional descriptive study. Day-care staff (N=206) from 33 day care centers in Seoul, completed a structured questionnaire. Results: Almost half of the day-care staff (53.4%) experienced sick events in their child at least once a month. The most frequently reported difficulty in having a sick child was in not having a health professional to consult, and almost all day-care staff (99.5%) had asked parents not to bring a sick child to the day care center. The biggest reason of exclusion was due to infectious disease including measles, chicken pox, mumps, diarrhea·vomiting, and pink eye. The majority of day-care staff (97.6%) agreed that there is a need for Center-based Child Care Health Program (CCCHP), and expected general health & illness management, and special care for chronic disease from CCCHP. Conclusion: These results showed a high incidence of sickness in children in day care centers, and there were great demands for a Center-based Child Care Health Program for day-care staff. Also the results of this study can be utilized as a basis for the establishment of child care policy in Korea.

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Educational Belief and Their Job Satisfaction of Day-Care Teachers (보육교사의 교육신념과 직무만족)

  • 임옥희
    • Korean Journal of Human Ecology
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    • v.7 no.1
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    • pp.101-111
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    • 2004
  • The purpose of this study is to compare overall educational belief and teacher's job satisfaction of day-care teachers. The subject of this were 112 day-care teachers in the Jeonbuk areas. The data were collected using Questionaries and analyzed by the statistical methods of frequencies, percentages, a Chi-square, ANOVA with SPSS pc program: The research Questions were as follows; 1. As the result of the investigation that what is the educational belief of the day-care theacher according to the marital status, academic background, the type of license, and the type of day-care centers\ulcorner 2. Is there any difference in teacher's job satisfaction of day-care teachers according to teachers education belief\ulcorner The main results of this study were as follows: 1. There was not difference in the teacher's educational belief of day-care teachers according to the marital status, academic background, the type of license, and the type of day-care centers in the Jeonbuk province. 2. Day-care teachers who have maturationism education belief was more than behaviorism education belief and interactionism education belief. 3. In the difference between teacher's belief of day-care teachers and job satisfaction of day-care teachers, the teacher who has interactionism, maturationism educationa belief has higher job satisfaction than the teacher who has behaviorism educational belief.

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Relationships between Young Children's Day Care Experience and Their Attachment Relationships with Parents and Socioemotional Behavior Problems (영유아기의 탁아 경험과 유아의 부모에 대한 애착 및 사회정서적 문제행동과의 관계)

  • Kim, Sook Ryong
    • Korean Journal of Child Studies
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    • v.18 no.2
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    • pp.5-18
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    • 1997
  • The purpose of the current study was to determine whether preschoolers' attachment relationships with their parents and socioemotional behaviors are related to their day care experiences in infancy and at the present time. The participants consisted of 59 preschoolers and their mothers from 2-parent, white, middle-class dual earner families in Madison, Wisconsin. Preschoolers' attachment quality was measured by two representational instruments: the Separation Anxiety Test and "My Family and Friends". Mothers completed questionnaires about their family background and their children's day care history. The quality of current day care was obtained from directors' reports about regulatable features of the day care centers. Teachers completed the Preschool Behavior Questionnaire. Data was analyzed by hierarchical multiple regression analysis. The results showed that the quality of preschoolers' attachment relationships with parents were significantly related to the amount of day care during infancy, the quality of current care, and children's gender. In addition, the amount of infant day care appeared to have negative relations with children's socioemotional behavior problems as evaluated by teachers.

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Effect of Circadian Rhythms on the Toluene Metabolism in Rats (흰쥐에 있어서 Toluene 대사에 미치는 주.야 시차의 영향)

  • 류종일;윤종국;신중규
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.67-74
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    • 1999
  • To investigate the effect of the circadian variations on the toluene metabolism, 50% toluene in olive oil (0.2 m1/100 g body weight) was intraperitoneally administered to the rats every other day for 6 days both in the night; 24:00 and the day; 12:00. Each group of animals was sacrificed at 8 hr after last injection of toluene. Hepatic microsomal aniline hydroxylase activity was more increased in control rats of night phase than those of day phase. On the other hand, the activities of hepatic benzylalcohol dehydrogenase in control rats of night phase showed the similiar value with that in those of day phase and in case of toluene treatment, these enzyme activities in rats of night phase were rather more decreased than those of day phase. Furthermore, hepatic benzaldehyde dehydrogenase activities were more or less higher in the control rats of night phase than those of day phase and by toluene treatment, enzyme activities of rats of night phase were somewhat decreased than those of day phase. in vitro, benzylalcohol or benzaldehyde inhibited the activities of benzylalcohol or aldehyde dehydrngenase prepared from the rats liver supematant. There were no differences in urinary hippuric acid contents between the night phase and day phase both in the control and toluene treated group. The increasing rate of liver weight per body weight (%), serum xanthine oxidase activities were higher in rats of night phase than in those of day phase by toluene treatment. In conclusion, these results indicate that the producing rate of benzylalcohol and benzaldehyde from toluene may be higher in rats of night phase than those of day phase.

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A Study on the Performance and the Space Use of Family Events (中都市 居住者의 家庭行事에 대한 硏究-거행 여부, 장소와 공간을 중심으로-)

  • 장상옥;신경주
    • Journal of the Korean housing association
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    • v.13 no.6
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    • pp.11-20
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    • 2002
  • The purpose of this study is to investigate the performance level of family events, the events places where the events occur. The subjects of 169 households were selected in Jinju city and data were gathered through a questionnaire method. The data were analyzed by the SPSS package program. The major findings are as follows; 1) Most of the families perform the majority of events except lpchun (the first day of spring), the vernal Equinox Day, the Autumnal Equinox Day, the Vega festival Day, Chilsuk, and the Respect-for-the-Aged Day. 2) Birthday parties score to the highest of all family events. 3) In general, the Lunar New Year's Day, the Full Moon Harvest Day, the ancestor worship ceremony, the 15th of January (in lunar calendar), the feast of a hundred day-old baby among lifetime ceremonies, family members'birthdays, housewarming parties among the other general celebrations, and Parents Day among memorial days are held in their homes. 4) The socialized celebration of the following family events is increasing: the first birthday party, banquets for parents'60th birthday, Christmas, and wedding anniversaries.