Acknowledgement
이 논문은 2005년 정부(교육인적자원부)의 재원으로 한국학술진흥재단의 지원을 받아 수행된 연구임(KRF-2005-042-G00044)
Effects of Fasting and High-fat Diet Feeding on Uncoupling Protein 3 mRNA Levels of Skeletal Muscle in Rats
절식과 고지방식 섭취가 골격근 UCP3 mRNA 발현에 미치는 영향
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Lim, Kiwon
(Department of Physical Education, Konkuk University)
;
- Hwang, Hye-Jung (Department of Physical Education, Konkuk University) ;
- Suh, Heajung (Bio-Food and Drug Research Center, Konkuk University) ;
- Tamura, Tomohiro (Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology)
- Received : 2009.05.13
- Accepted : 2009.05.25
- Published : 2009.06.30
Abstract
PURPOSE. The purpose of this study was to investigate the effects of fasting and high-fat diet feeding on uncoupling protein 3 (UCP3) mRNA levels, uncoupling the respiratory chain and producing heat, of skeletal muscle in rat. METHODS. Fasting experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into non-fasting groups (CON) and fasting groups (FG) for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of CON were sacrificed at 0 and 3 day. High-fat diet experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into low-fat diet groups (LF) and high-fat diet group feeding for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of LF were sacrificed at 0 and 3 day. Analysis: Analysis of UCP3 mRNA expression was used by Real-time PCR. RESULTS. UCP3 mRNA levels of FG group were increased according to time course for 2 days- fasting but decreased at 3 day-fasting. UCP3 mRNA of HF were increased during HF diet feeding for 2 day, and peaked at 1 day-HF feeding, but decreased 2 day and 3 day-HF feeding CONCLUSION. Therefore, it may be rational that UCP3 is up-regulation when a large amount of fatty acids influx occurs in skeletal muscles as well as might have a role for fine adjustments of energy expenditure.