• 한국약용작물학회지
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• 제23권2호
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• pp.144-149
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• 2015

The peel of Citrus sunki exhibits multiple biological activities such as anti-oxidant, anti-inflammation and anti-obesity, but little is known about neurodegeneration-related activities. In this study, we investigated the protective effect of ethanolic extract from both immature and mature Citrus sunki peel on neuronal cell death. Treatment of the neuroblastoma cell line SH-SY5Y with $MPP^+$, an inducer of Parkinson disease model, increased cell death in a dose dependent manner. Increased levels of active caspase-3 and cleaved PARP were detected. Treatment with immature Citrus sunki peel extract significantly reduced $MPP^+$-induced neurotoxicity. Cytoprotection with immature Citrus sunki peel extract was associated with a decrease in caspase-3 activation and PARP cleavage. In contrast, mature Citrus sunki peel extract had no significant effects. These data suggest that immature Citrus sunki peel extract may exert anti-apoptotic effect through the inhibition of caspase-3 signaling pathway on $MPP^+$-induced neuronal cell death.

• 생명과학회지
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• 제13권4호
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• pp.492-497
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• 2003

액체배양을 이용한 잣버섯(Lentinus lepideus KACC 50120)의 균사체 생육에 관한 결과로는 최적온도는 $25^{\circ}C$이고, 최적 pH는 5.5이었다. 공시배지중에서 최대 균사 생육을 보인 최적배지는 YMG 배지로 조사되었다. YMG 배지를 기본배지로 하여 영양 요구성 실험을 한 결과 탄소원 선발 및 최적 농도에서는 최적 탄소원은 glucose이고 최적 농도는 4.0%(w/v)이며 질소원 선발 및 최적 농도에서는 malt extract와 yeast extract를 혼합하여 사용하였을 때 최대 균사 생육을 보였고, 상기의 두가지 복합질소원을 1:1의 무게비로 혼합하여 1.5%(w/v)농도로 첨가하였을 때 균사생육이 가장 양호하였다.

• 한방비만학회지
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• 제18권1호
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• pp.19-26
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• 2018

Objectives: Alcoholic fatty liver is a potentially pathologic condition which can progress to steatohepatitis, fibrosis, and cirrhosis. The objective of this study is to investigate the effects of Scutellaria Radix (SR) extract on the alcoholic fatty liver induced by long-term EtOH administration. Results: Male Sprague Dawley rats were used in this study. All animals were randomly divided into Normal group, treated with saline (n=10); EtOH group, treated with ethanol (n=10); EtOH+SR group, treated with ethanol+Scutellaria Radix extract (n=10). For oral administration of ethanol in EtOH and EtOH+SR group, the ethanol was dissolved in distilled water in concentrations of 25% (v/v). Throughout the experiment of 8 week, the rats were allowed free access to water and standard chow. Sample group were administrated by Scutellaria Radix extract daily for 8 weeks. Results: The levels of hepatic marker such as aspartate aminotransferase and alanine aminotransferase were altered. Histopathological changes such as ballooning, fatty and hydropic degeneration were reduced and the expression of tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) was significantly attenuated by Scutellaria Radix extract. Conclusions: These data suggest that Scutellaria Radix extract attenuated the alcoholic simple fatty liver by improving hepatic lipid metabolism via suppression of $TNF-{\alpha}$ protein. Scutellaria Radix could be effective in protecting the liver from alcoholic fatty liver.

• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1827-1834
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• 2020

Candida albicans is a major fungal pathogen in humans. In our previous study, we reported that an ethanol extract from Aucklandia lappa weakens C. albicans cell wall by inhibiting synthesis or assembly of both (1,3)-β-D-glucan polymers and chitin. In the current study, we found that the extract is involved in permeabilization of C. albicans cell membranes. While uptake of ethidium bromide (EtBr) was 3.0% in control cells, it increased to 7.4% for 30 min in the presence of the A. lappa ethanol extract at its minimal inhibitory concentration (MIC), 0.78 mg/ml, compared to uptake by heat-killed cells. Besides, leakage of DNA and proteins was observed in A. lappa-treated C. albicans cells. The increased uptake of EtBr and leakage of cellular materials suggest that A. lappa ethanol extract induced functional changes in C. albicans cell membranes. Incorporation of diphenylhexatriene (DPH) into membranes in the A. lappa-treated C. albicans cells at its MIC decreased to 84.8%, after 60 min of incubation, compared with that of the controls, indicate that there was a change in membrane dynamics. Moreover, the anticandidal effect of the A. lappa ethanol extract was enhanced at a growth temperature of 40℃ compared to that at 35℃. The above data suggest that the antifungal activity of the A. lappa ethanol extract against C. albicans is associated with synergistic action of membrane permeabilization due to changes in membrane dynamics and cell wall damage caused by reduced formation of (1,3)-β-D-glucan and chitin.

• 한국식품영양학회지
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• 제19권3호
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• pp.271-278
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• 2006

RPSO, SO, PNO의 3종 향미유에 대하여 로즈마리 유기 용매 추출물과 비교군으로 토코페롤을 각각 1,000ppm씩 동량으로 적용하여 60${\pm}$2$^{\circ}C$의 항온기에 저장하여 AV, POV 및 카르보닐 화합물 생성량의 변화 양상을 측정하였다. 이들 향미유의 AV, POV측정 과정에서는 고유의 색상으로 인하여 상대적으로 측정 오차가 크게 발생하였으며, 토코페롤은 AV, POV, 카르보닐 화합물 생성량 등 각종 측정 항목에서 항산화제로서의 역할보다는 오히려 산화 촉진제로 작용하는 것으로 밝혀졌다. 전체적으로 chloroform/MeOH 추출물 처리의 저장 안정성의 향상 효과가 가장 우수하였으며, 측정 항목 및 추출물별 저장 안점성의 향상 효과는 다음과 같았다. 즉, AV는 chloroform/ MeOH 추출물>ethyl alcohol 추출물>열수 추출물>ethyl acetate 추출물>acetone 추출물>무처리군>토코페롤 처리군이었으며, POV는 chloroform/MeOH 추출물>ethyl alcohol추출물${\geq}$ethyl acetate 추출물>acetone 추출물${\geq}$열수 추출물>무처리군>토코페롤 처리군이었고, 카르보닐 화합물 생성량은 chloroform/ MeOH 추출물>ethyl acetate추출물>ethyl alcohol 추출물>열수 추출물>acetone 추출물>무처리군>토코페롤 처리군의 순이었다.

• International Journal of Computer Science & Network Security
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• 제23권3호
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• pp.10-16
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• 2023

The ever-increasing amount of data generated by various industries and systems has led to the development of data mining techniques as a means to extract valuable insights and knowledge from such data. The electrical energy industry is no exception, with the large amounts of data generated by SCADA systems. This study focuses on the analysis of historical data recorded in the SCADA database of the Libyan Electricity Company. The database, spanned from January 1st, 2013, to December 31st, 2022, contains records of daily date and hour, energy production, temperature, humidity, wind speed, and energy consumption levels. The data was pre-processed and analyzed using the WEKA tool and the Apriori algorithm, a supervised machine learning technique. The aim of the study was to extract association rules that would assist decision-makers in making informed decisions with greater efficiency and reduced costs. The results obtained from the study were evaluated in terms of accuracy and production time, and the conclusion of the study shows that the results are promising and encouraging for future use in the Libyan Electricity Company. The study highlights the importance of data mining and the benefits of utilizing machine learning technology in decision-making processes.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 추계학술대회
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• pp.235-239
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• 2010

휴대폰 사용이 포화상태에 이르고, 최근 iPhone을 비롯한 Smart Phone의 사용이 급격히 증가하고 있다. 현재 휴대폰에서 포렌식 자료 추출하는 방법은 SYN, JTAG, Revolving 세가지 방법이 있다. 하지만 휴대폰과 Smart Phone의 기술과 사용방법의 차이로 인하여 포렌식 자료 추출 방법도 달라야 한다. 따라서 본 논문에서는 Smart Phone에서의 포렌식 자료 추출 방법을 연구하고자 한다. Smart Phone에서는 많이 사용되는 Google Android와 Windows Mobile Smart Phone의 분석을 위하여 스펙과 O.S. 분석, 데이터 분석을 한다. 또한 Google Android와 Windows Mobile Smart Phone의 포렌식 자료를 추출하여 증거자료를 생성한다. 본 연구를 통하여 실험된 기술 연구는 모바일 Smart Phone 포렌식 기술 발전에 기여할 것이다.

• 한국식품저장유통학회지
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• 제16권3호
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• pp.392-399
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• 2009

녹차는 생리활성의 촉진작용뿐아니라 항암, 항산화, 고혈압 억제효과, 체중조절 등 다방면의 약리효과를 보이는 것으로 보고되고 있다. 아울러, 녹차의 항균 및 항진균 작용에 관한 연구결과도 발표되고 있으나, 항균작용 기작에 접근한 기초 연구는 별로 많지 않다. 본 연구는 식품변패미생물에 대한 녹차추출물의 항균작용을 구명하는 기초자료를 획득하는데 그 목적이 있다. 녹차추출물의 항균력을 조사한 결과, $500{\mu}g/mL$ 이상의 농도에서 뚜렷한 항균활성을 보였고, 광역의 병원성 및 부패성 미생물들에 대하여 항균활성을 나타내었다. 또한, 녹차추출물의 항균물질은 넓은 범위의 온도($40{\sim}150^{\circ}C$) 및 pH($3{\sim}11$)에서 안정성을 나타냈다. 따라서 녹차추출물의 항균물질은 항균활성이 높고, 항균 대상 미생물의 분야가 광범위할 뿐아니라, 넓은 범위의 온도 및 pH에 안정하여 이상적인 천연 항균제로서의 개발가능성을 제시하였다. 아울러, 녹차추출물의 항균작용을 알기 위하여, 녹차추출물 처리가 미생물 세포막 기능에 미치는 영향을 조사한 결과, 녹차추출물의 주요 작용기작은 세포막 perturbation에 기인한 것으로 사료되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.1891-1898
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• 2016

Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India. Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines. To our knowledge, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. cylindrica using an oral squamous cell carcinoma cell line SCC-9 as an in vitro model system. A methanol extract from dried leaves of I. cylindrica (ICL) was prepared by standard procedures. Effects of the ICL extract on the morphology of SCC-9 cells was visualized by microscopy. Cytotoxicity was determined by MTT assay. Effects of the ICL extract on colony forming ability of SCC-9 cells was evaluated using clonogenic assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay. The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. This treatment also significantly reduced the clonogenic potential and inhibited cell proliferation by arresting the cell cycle in the G2/M phase. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis. This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. cylindrica in human oral cancer cell line. Our data indicates that ICL extract could be considered as one of the lead compounds for the formulation of anticancer therapeutic agents to treat/manage human oral cancers. The natural abundance of I. cylindrica and its wide geographic distribution could render it one of the primary resource materials for preparation of anticancer therapeutic agents.

• 생약학회지
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• 제44권3호
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• pp.269-274
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• 2013

In this study, we investigated the anticancer effect of rice bran extract in HL-60 human promyelocytic leukemia cells. The extract of rice bran inhibited the proliferation of HL-60 cells. When treated with the rice bran extract, we could observe the apoptotic characteristics such as apoptotic bodies and the increase of sub-G1 hypodiploid cell population, increase of Bax level, decrease of Bcl-2 expression, cleavage of procaspase-3, cleavage of procaspase-9 and cleavage of poly(ADP-ribose) polymerase(PARP) in HL-60 cells. Furthermore, the apoptosis induction of HL-60 cells treated with the rice bran extract was also accompanied by the inactivation of mitogen-activated protein kinases (MAPK) such as ERK1/2 MAPK and p38 MAPK. In addition, the rice bran extract induced the down-regulation of c-myc. These data suggested that the rice bran extract could induce the apoptosis via the inactivation of ERK1/2 MAPK and p38 MAPK, and the down-regulation of c-myc in HL-60 acute pomyelocytic leukemia cells. The results support that the rice bran extract might have potential for the treatment of acute promyelocytic leukemia.